| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,975,241 | T→G | 43.3% | L684R (CTG→CGG) | sasA_19 → | Adaptive‑response sensory‑kinase SasA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,975,241 | 0 | T | G | 43.3% | 12.3 / 2.4 | 28 | L684R (CTG→CGG) | sasA_19 | Adaptive‑response sensory‑kinase SasA |
| Reads supporting (aligned to +/- strand): ref base T (2/9); new base G (5/4); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTC > KT2440/5975109‑5975353 | aaGCCTTGATTGGCGCGGGCTTCCTGCAACCGGGCCAGAACGGCTGGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTgg < 8:347200/135‑1 (MQ=255) gCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTg > 4:316511/1‑109 (MQ=255) gCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGGGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTg < 3:316511/109‑1 (MQ=255) cAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATg > 4:37575/1‑114 (MQ=255) cAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATg < 3:37575/114‑1 (MQ=255) gggCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCCAGCGCCCCGCATGGCCCCGGCGacacca > 6:292043/1‑130 (MQ=255) ggCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCAATCGCCCGGCTTGGCCACGGCGAGCCCACCAGGggttg > 5:62461/1‑135 (MQ=255) cTCATGCCGGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCGGCATGCACGTATCGCCCGGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCgcc < 2:2866/134‑1 (MQ=255) gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCGTGCACGTATCGCCCCGGCCCAGCCCCAGGCGTGGGCCGGGGGGACCCCCGCGGGGTTGGTCGAGCTAGCCCCCCAGCGTCAAcc > 6:291663/1‑134 (MQ=255) gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGGCCAGCGCCCCGCGTGGCCAGGGCGCACGCCCCCGGGGTTGTTGGAGCGCGCCCGCCCCCaacaacc > 2:81180/1‑134 (MQ=255) gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCAGTCGCCAGGCTTGGCCAGGGCGCACGCACGAGGTGTTGTTGGCGCTCGCCAGCCCGCGACACcg > 7:371872/1‑135 (MQ=255) gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAAGCCCAGCGCCCCGCGTGGGCCCGGGGCACGCCCGACGTGGTGTTGGCGCTCCCCACGCAGCAAAAAcc > 6:355767/1‑134 (MQ=255) aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCCAGCGCCACGCGTGGGCCCGGCGCCCCCCCGCGGGGTTGTTGGAGCGCGCCCCCCCGCGACCAcgc > 5:163419/1‑135 (MQ=255) aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCAAGCGCCACGCGTGGCCCGGGGGCACGCCAGAGGGGTTGTTCGCGCGCGCCCCCCCGCTACAAccc > 2:280998/1‑133 (MQ=255) ccGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCAGGCGTGGGCCGGGCGCCCGCCCGCGGTGTGGGTGGCGCTCGCCCGCCCGCGACCACCCGcccccc > 7:303775/1‑131 (MQ=255) cTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCCGGCTTGGCCAGGGCGCTCGCCCCCGGGGTTGTTGGCGCGCGCCCCCCCGCCACCACGCGCCAGccccgc > 4:70361/1‑134 (MQ=255) aCGCCCGTGCACCACCGCGCCTCGCGGCATGCACGTATCGCCCTGGCCTTGCGCCAGGCTGGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCa < 7:88736/135‑1 (MQ=255) cGCCGGTGCACCCGCCCGCCTCGCTGAATGCACGTAGCGCGCGGGCCATGCGCGTGGGTTGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGc < 3:352799/136‑1 (MQ=255) cGCCCGGGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGc < 3:282420/136‑1 (MQ=255) cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGGCCAGCGCCCCGCGTGGGCCGGGCGCACGCCCGCGGGGTTGGTGGCGCTCGCCCCCCCGCGCCACCGCGCCCCCCCCGGCGGGGTTggggcg > 6:135516/1‑130 (MQ=255) cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCCGGCGTGGGCCGGGGGGACGCCCGAGGTGGTGTTGGCGCGCGCCAGCCCGCGACCACCCGCCCGCCCCCGCGGGTTTTGAGCg > 6:107878/1‑135 (MQ=255) ccGTGCCCCAGCGCGCCGCGCTGCAGGCACGTAGCGCCCGGCCCATGCGCCCGGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCgg < 8:228724/135‑1 (MQ=255) cgcgcgcCTCGATGCAGGCACGTATCGCCCGGGCGATGAGGTTGGGTGGGCGTGGGCTAACGCACGAGTTGTTGTTCGAGCTCTCGAGCCAGCTACAACTCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTgag < 8:253211/134‑3 (MQ=255) cgcgcCTCGCTGCAGGCCCGTAGCGCCCTGCCCAGGCGCCAGGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGaa < 6:258514/135‑1 (MQ=255) cgcgcCTCGCTGCAGGCACGACTCCCCCTGCCCAGGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGGTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGaa < 6:253785/135‑1 (MQ=255) cgcCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCCGGGGTTGCGCCGGCGCACGCCCCCGGGGTTTTTGGCGCGGGCCCGGCCGCGACCACGCGCCACCCCCGGCGGGTGTGGAGCGGGTGTCGGCGCAGc > 8:263282/1‑135 (MQ=255) cgcGCTGCATGCAGGTTTCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc < 3:179978/134‑1 (MQ=255) tCGCTGCTGGCAGGTATCGCCCTGGCCATGCGCCTGGCTTGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc < 8:180772/135‑1 (MQ=255) | AAGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTC > KT2440/5975109‑5975353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |