Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,975,263 A→C 34.0% P691P (CCA→CCC)  sasA_19 → Adaptive‑response sensory‑kinase SasA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,975,2630AC34.0% 18.5 / 4.3 30P691P (CCA→CCC) sasA_19Adaptive‑response sensory‑kinase SasA
Reads supporting (aligned to +/- strand):  ref base A (10/6);  new base C (9/2);  total (19/11)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGG  >  KT2440/5975140‑5975397
                                                                                                                           |                                                                                                                                      
gggCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCCAGCGCCCCGCATGGCCCCGGCGacacca                                                                                                                             >  6:292043/1‑130 (MQ=255)
          ggCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCAATCGCCCGGCTTGGCCACGGCGAGCCCACCAGGggttg                                                                                                                   >  5:62461/1‑135 (MQ=255)
                       cTCATGCCGGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCGGCATGCACGTATCGCCCGGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCgcc                                                                                                       <  2:2866/134‑1 (MQ=255)
                                    gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCGTGCACGTATCGCCCCGGCCCAGCCCCAGGCGTGGGCCGGGGGGACCCCCGCGGGGTTGGTCGAGCTAGCCCCCCAGCGTCAAcc                                                                                         >  6:291663/1‑134 (MQ=255)
                                    gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGGCCAGCGCCCCGCGTGGCCAGGGCGCACGCCCCCGGGGTTGTTGGAGCGCGCCCGCCCCCaacaacc                                                                                         >  2:81180/1‑134 (MQ=255)
                                    gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCAGTCGCCAGGCTTGGCCAGGGCGCACGCACGAGGTGTTGTTGGCGCTCGCCAGCCCGCGACACcg                                                                                         >  7:371872/1‑135 (MQ=255)
                                    gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAAGCCCAGCGCCCCGCGTGGGCCCGGGGCACGCCCGACGTGGTGTTGGCGCTCCCCACGCAGCAAAAAcc                                                                                         >  6:355767/1‑134 (MQ=255)
                                     aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCCAGCGCCACGCGTGGGCCCGGCGCCCCCCCGCGGGGTTGTTGGAGCGCGCCCCCCCGCGACCAcgc                                                                                        >  5:163419/1‑135 (MQ=255)
                                     aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCAAGCGCCACGCGTGGCCCGGGGGCACGCCAGAGGGGTTGTTCGCGCGCGCCCCCCCGCTACAAccc                                                                                        >  2:280998/1‑133 (MQ=255)
                                            ccGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCAGGCGTGGGCCGGGCGCCCGCCCGCGGTGTGGGTGGCGCTCGCCCGCCCGCGACCACCCGcccccc                                                                                 >  7:303775/1‑131 (MQ=255)
                                               cTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCCGGCTTGGCCAGGGCGCTCGCCCCCGGGGTTGTTGGCGCGCGCCCCCCCGCCACCACGCGCCAGccccgc                                                                             >  4:70361/1‑134 (MQ=255)
                                                          aCGCCCGTGCACCACCGCGCCTCGCGGCATGCACGTATCGCCCTGGCCTTGCGCCAGGCTGGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCa                                                                   <  7:88736/135‑1 (MQ=255)
                                                           cGCCGGTGCACCCGCCCGCCTCGCTGAATGCACGTAGCGCGCGGGCCATGCGCGTGGGTTGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGc                                                                 <  3:352799/136‑1 (MQ=255)
                                                           cGCCCGGGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGc                                                                 <  3:282420/136‑1 (MQ=255)
                                                             cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGGCCAGCGCCCCGCGTGGGCCGGGCGCACGCCCGCGGGGTTGGTGGCGCTCGCCCCCCCGCGCCACCGCGCCCCCCCCGGCGGGGTTggggcg                                                                >  6:135516/1‑130 (MQ=255)
                                                             cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCCGGCGTGGGCCGGGGGGACGCCCGAGGTGGTGTTGGCGCGCGCCAGCCCGCGACCACCCGCCCGCCCCCGCGGGTTTTGAGCg                                                                >  6:107878/1‑135 (MQ=255)
                                                              ccGTGCCCCAGCGCGCCGCGCTGCAGGCACGTAGCGCCCGGCCCATGCGCCCGGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCgg                                                               <  8:228724/135‑1 (MQ=255)
                                                                       cgcgcgcCTCGATGCAGGCACGTATCGCCCGGGCGATGAGGTTGGGTGGGCGTGGGCTAACGCACGAGTTGTTGTTCGAGCTCTCGAGCCAGCTACAACTCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTgag                                                      <  8:253211/134‑3 (MQ=255)
                                                                         cgcgcCTCGCTGCAGGCCCGTAGCGCCCTGCCCAGGCGCCAGGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGaa                                                    <  6:258514/135‑1 (MQ=255)
                                                                         cgcgcCTCGCTGCAGGCACGACTCCCCCTGCCCAGGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGGTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGaa                                                    <  6:253785/135‑1 (MQ=255)
                                                                           cgcCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCCGGGGTTGCGCCGGCGCACGCCCCCGGGGTTTTTGGCGCGGGCCCGGCCGCGACCACGCGCCACCCCCGGCGGGTGTGGAGCGGGTGTCGGCGCAGc                                                  >  8:263282/1‑135 (MQ=255)
                                                                              cgcGCTGCATGCAGGTTTCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc                                              <  3:179978/134‑1 (MQ=255)
                                                                               tCGCTGCTGGCAGGTATCGCCCTGGCCATGCGCCTGGCTTGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc                                              <  8:180772/135‑1 (MQ=255)
                                                                                                               ccAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc              >  6:166267/1‑135 (MQ=255)
                                                                                                               ccAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc              >  5:74530/1‑135 (MQ=255)
                                                                                                               ccAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCa             >  3:367347/1‑136 (MQ=255)
                                                                                                                    cTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcg         >  6:35068/1‑135 (MQ=255)
                                                                                                                    cTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcg         >  7:158394/1‑135 (MQ=255)
                                                                                                                     ttGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcga        >  6:206235/1‑135 (MQ=255)
                                                                                                                        gCCCGGGCCAACGCACGAGGTGGTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcgacgc     <  5:259027/135‑1 (MQ=255)
                                                                                                                           aGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCggg  >  6:234797/1‑135 (MQ=255)
                                                                                                                           |                                                                                                                                      
GGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGG  >  KT2440/5975140‑5975397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑