Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,975,297 A→C 19.5% S703R (AGC→CGC)  sasA_19 → Adaptive‑response sensory‑kinase SasA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,975,2970AC19.5% 93.3 / 2.3 44S703R (AGC→CGC) sasA_19Adaptive‑response sensory‑kinase SasA
Reads supporting (aligned to +/- strand):  ref base A (19/15);  new base C (8/2);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCTGGTTACCTGCACAC  >  KT2440/5975176‑5975430
                                                                                                                         |                                                                                                                                     
gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCGTGCACGTATCGCCCCGGCCCAGCCCCAGGCGTGGGCCGGGGGGACCCCCGCGGGGTTGGTCGAGCTAGCCCCCCAGCGTCAAcc                                                                                                                          >  6:291663/1‑134 (MQ=255)
gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGGCCAGCGCCCCGCGTGGCCAGGGCGCACGCCCCCGGGGTTGTTGGAGCGCGCCCGCCCCCaacaacc                                                                                                                          >  2:81180/1‑134 (MQ=255)
gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCAGTCGCCAGGCTTGGCCAGGGCGCACGCACGAGGTGTTGTTGGCGCTCGCCAGCCCGCGACACcg                                                                                                                          >  7:371872/1‑135 (MQ=255)
gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAAGCCCAGCGCCCCGCGTGGGCCCGGGGCACGCCCGACGTGGTGTTGGCGCTCCCCACGCAGCAAAAAcc                                                                                                                          >  6:355767/1‑134 (MQ=255)
 aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCCAGCGCCACGCGTGGGCCCGGCGCCCCCCCGCGGGGTTGTTGGAGCGCGCCCCCCCGCGACCAcgc                                                                                                                         >  5:163419/1‑135 (MQ=255)
 aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCAAGCGCCACGCGTGGCCCGGGGGCACGCCAGAGGGGTTGTTCGCGCGCGCCCCCCCGCTACAAccc                                                                                                                         >  2:280998/1‑133 (MQ=255)
        ccGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCAGGCGTGGGCCGGGCGCCCGCCCGCGGTGTGGGTGGCGCTCGCCCGCCCGCGACCACCCGcccccc                                                                                                                  >  7:303775/1‑131 (MQ=255)
           cTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCCGGCTTGGCCAGGGCGCTCGCCCCCGGGGTTGTTGGCGCGCGCCCCCCCGCCACCACGCGCCAGccccgc                                                                                                              >  4:70361/1‑134 (MQ=255)
                      aCGCCCGTGCACCACCGCGCCTCGCGGCATGCACGTATCGCCCTGGCCTTGCGCCAGGCTGGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCa                                                                                                    <  7:88736/135‑1 (MQ=255)
                       cGCCGGTGCACCCGCCCGCCTCGCTGAATGCACGTAGCGCGCGGGCCATGCGCGTGGGTTGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGc                                                                                                  <  3:352799/136‑1 (MQ=255)
                       cGCCCGGGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGc                                                                                                  <  3:282420/136‑1 (MQ=255)
                         cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGGCCAGCGCCCCGCGTGGGCCGGGCGCACGCCCGCGGGGTTGGTGGCGCTCGCCCCCCCGCGCCACCGCGCCCCCCCCGGCGGGGTTggggcg                                                                                                 >  6:135516/1‑130 (MQ=255)
                         cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCCGGCGTGGGCCGGGGGGACGCCCGAGGTGGTGTTGGCGCGCGCCAGCCCGCGACCACCCGCCCGCCCCCGCGGGTTTTGAGCg                                                                                                 >  6:107878/1‑135 (MQ=255)
                          ccGTGCCCCAGCGCGCCGCGCTGCAGGCACGTAGCGCCCGGCCCATGCGCCCGGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCgg                                                                                                <  8:228724/135‑1 (MQ=255)
                                   cgcgcgcCTCGATGCAGGCACGTATCGCCCGGGCGATGAGGTTGGGTGGGCGTGGGCTAACGCACGAGTTGTTGTTCGAGCTCTCGAGCCAGCTACAACTCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTgag                                                                                       <  8:253211/134‑3 (MQ=255)
                                     cgcgcCTCGCTGCAGGCCCGTAGCGCCCTGCCCAGGCGCCAGGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGaa                                                                                     <  6:258514/135‑1 (MQ=255)
                                     cgcgcCTCGCTGCAGGCACGACTCCCCCTGCCCAGGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGGTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGaa                                                                                     <  6:253785/135‑1 (MQ=255)
                                       cgcCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCCGGGGTTGCGCCGGCGCACGCCCCCGGGGTTTTTGGCGCGGGCCCGGCCGCGACCACGCGCCACCCCCGGCGGGTGTGGAGCGGGTGTCGGCGCAGc                                                                                   >  8:263282/1‑135 (MQ=255)
                                          cgcGCTGCATGCAGGTTTCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc                                                                               <  3:179978/134‑1 (MQ=255)
                                           tCGCTGCTGGCAGGTATCGCCCTGGCCATGCGCCTGGCTTGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc                                                                               <  8:180772/135‑1 (MQ=255)
                                                                           ccAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc                                               >  5:74530/1‑135 (MQ=255)
                                                                           ccAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc                                               >  6:166267/1‑135 (MQ=255)
                                                                           ccAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCa                                              >  3:367347/1‑136 (MQ=255)
                                                                                cTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcg                                          >  7:158394/1‑135 (MQ=255)
                                                                                cTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcg                                          >  6:35068/1‑135 (MQ=255)
                                                                                 ttGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcga                                         >  6:206235/1‑135 (MQ=255)
                                                                                    gCCCGGGCCAACGCACGAGGTGGTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcgacgc                                      <  5:259027/135‑1 (MQ=255)
                                                                                    cccggggCCAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcgacgc                                      <  2:173850/131‑1 (MQ=255)
                                                                                    ccccgggCGAACGCACGAGGTGTTGGTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCacgcgacgc                                      <  5:115226/131‑1 (MQ=255)
                                                                                       aGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCggg                                   >  6:234797/1‑135 (MQ=255)
                                                                                            gAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCtt                             >  3:266691/1‑136 (MQ=255)
                                                                                            gAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCt                              >  1:100073/1‑135 (MQ=255)
                                                                                            gAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCt                              >  7:284742/1‑135 (MQ=255)
                                                                                                gCACGTGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGa                          <  5:292043/135‑1 (MQ=255)
                                                                                                   ctaGGTGTTGTTCGAGCTCGCCAGCCAGCTACACCGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCa                       >  5:123747/3‑135 (MQ=255)
                                                                                                      ggTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCCCCTTCGAGCAGGcc                   >  3:234626/1‑136 (MQ=255)
                                                                                                        tgtggtTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGAGGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCg                  <  2:36289/135‑1 (MQ=255)
                                                                                                          gtgttCGAGCTCGCCCGCCAGCTACACCGCGCCAGCCCGTGCGGGTTTGCAGCGGATGACTGCGAAGGGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCt                <  6:263097/134‑1 (MQ=255)
                                                                                                             ttCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCTgg              >  1:50220/1‑134 (MQ=255)
                                                                                                              tCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCTGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACTCGGGCACCTTCGAGCAGGCCGCTGGt             <  6:355982/134‑1 (MQ=255)
                                                                                                               cGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCTGGTTa           >  2:316112/1‑135 (MQ=255)
                                                                                                                  gcgcgcccgccAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCTAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCTGGTTACCt        <  1:370876/132‑1 (MQ=255)
                                                                                                                  gCTCGCCCGCCAGATACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCAGGCGACGCGGGCACCTTCGAGCAGGCCGCTGGTTACCt        <  2:226830/135‑1 (MQ=255)
                                                                                                                     cGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCTGGTTACCTGca     >  2:37617/1‑135 (MQ=255)
                                                                                                                     cGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCTGGTTACCTGca     >  2:136643/1‑135 (MQ=255)
                                                                                                                        cagccagcTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCTGGTTACCTGCacac  <  5:234797/135‑1 (MQ=255)
                                                                                                                         |                                                                                                                                     
GATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCACGCGACGCGGGCACCTTCGAGCAGGCCGCTGGTTACCTGCACAC  >  KT2440/5975176‑5975430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑