Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,005,873 C→T 7.4% A297V (GCG→GTG)  rep_2 → ATP‑dependent DNA helicase Rep

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,005,8730CT 7.4% 169.4 / 2.1 54A297V (GCG→GTG) rep_2ATP‑dependent DNA helicase Rep
Reads supporting (aligned to +/- strand):  ref base C (22/28);  new base T (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCCGAGAACCTGATGCTGCTCAAGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCTTGCACCTGCGCACCAACCGCCCGT  >  KT2440/6005744‑6006007
                                                                                                                                 |                                                                                                                                      
gcCCCGAGAACCTGATGCTGCTCAAGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCg                                                                                                                                   <  8:164518/135‑1 (MQ=255)
    cGAGAACCTGATGCTGCTCAAGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAag                                                                                                                              <  4:17210/136‑1 (MQ=255)
         aCCTGATGCTGCTCAAGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAagcagc                                                                                                                          <  5:354220/135‑1 (MQ=255)
               tgctgcTCAAGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCtgtg                                                                                                                      <  1:218877/133‑1 (MQ=255)
               tgctgcTCAAGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGa                                                                                                                    >  8:218796/1‑135 (MQ=255)
                  tgctCAAGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCg                                                                                                                 <  2:118322/135‑1 (MQ=255)
                       aaGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAATAGCAGCTGTGGAGCGAAAtg                                                                                                            >  5:48475/1‑135 (MQ=255)
                       aaGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAAtg                                                                                                            >  5:179769/1‑135 (MQ=255)
                       aaGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAAACCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAAtgg                                                                                                           >  3:304239/1‑136 (MQ=255)
                                 aCCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAAtgggcgtgggcg                                                                                                  >  1:18243/1‑135 (MQ=255)
                                 aCCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAAtgggcgtgggcg                                                                                                  >  7:340173/1‑135 (MQ=255)
                                   ccctccctGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGcgacg                                                                                               <  3:17307/136‑1 (MQ=255)
                                    cctccctGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGcgacga                                                                                              <  3:112792/136‑1 (MQ=255)
                                    cctccctGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGcga                                                                                                 >  8:296259/1‑133 (MQ=255)
                                     ctccctGAAGATCGTCATGCTCTAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGcgacga                                                                                              <  5:93673/135‑1 (MQ=255)
                                             agctCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCAGGTGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGCCGAGATCcgct                                                                                      >  8:205129/4‑134 (MQ=255)
                                             aGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGACGCATCCGGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGTGGTCGAAAAGCAGCTGTGGAGCGACATGGGCGTGGGCGACTAGATCcgcg                                                                                      >  8:199830/1‑135 (MQ=255)
                                               aTCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTg                                                                                    <  2:21544/135‑1 (MQ=255)
                                                 cGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGAt                                                                                  <  7:287971/135‑1 (MQ=255)
                                                   tCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATcc                                                                                >  2:190374/1‑135 (MQ=255)
                                                     aTGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGc                                                                              <  1:193257/135‑1 (MQ=255)
                                                     aTGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGc                                                                              >  2:359345/1‑135 (MQ=255)
                                                             aCAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAAc                                                                     >  4:225672/1‑136 (MQ=255)
                                                              cAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGGTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAAc                                                                     <  7:65554/135‑1 (MQ=255)
                                                              cAGAACTACCGTGCCACCAGCCGCATCCTGCGCTGCGCCAACGGGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAAc                                                                     <  7:173132/135‑1 (MQ=255)
                                                                    tACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGaa                                                               <  5:357484/135‑1 (MQ=255)
                                                                      ccGGTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAg                                                              <  4:115250/134‑1 (MQ=255)
                                                                         ttCCACCAGCCGCAGCCTGCGCTGCGCCAACGTGTAGATCGCCAACAATCCGCATGCGATCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAg                                                         <  4:279444/136‑1 (MQ=255)
                                                                              ccaGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGcc                                                      >  5:311013/1‑134 (MQ=255)
                                                                              ccaGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGACAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGCGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGcc                                                      >  6:188466/1‑134 (MQ=255)
                                                                                  ccGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGGGGTGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAAcg                                                 <  5:229145/135‑1 (MQ=255)
                                                                                          tgcgctgcgcCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGcc                                          <  5:314923/134‑1 (MQ=255)
                                                                                            cgctgcgcCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCAt                                        <  3:69528/134‑1 (MQ=255)
                                                                                               tgcgcCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGc                                           <  5:254547/128‑1 (MQ=255)
                                                                                               tgcgcCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGc                                           >  6:254547/1‑128 (MQ=255)
                                                                                               tgcgcCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGaa                                    >  7:2137/1‑135 (MQ=255)
                                                                                                 cgcCAACGTGTTGATCGCAAACAATCCGCAGGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAAt                                  <  1:149844/135‑1 (MQ=255)
                                                                                                 cgcCAACGTGGTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAAt                                  <  5:270261/135‑1 (MQ=255)
                                                                                                  gcCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAAt                                  <  5:138614/134‑1 (MQ=255)
                                                                                                   ccAACGTGTTGATCGCCACCAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATcc                                >  8:218144/1‑135 (MQ=255)
                                                                                                    cAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGcgacg                                                                                               <  7:342478/71‑1 (MQ=255)
                                                                                                    cAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGcgacg                                                                                               >  8:342478/1‑71 (MQ=255)
                                                                                                    cAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATcc                                >  2:196798/1‑134 (MQ=255)
                                                                                                    cAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCt                               >  2:173239/1‑135 (MQ=255)
                                                                                                    cAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTc                              >  4:341376/1‑136 (MQ=255)
                                                                                                      aCGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCa                             <  2:19075/135‑1 (MQ=255)
                                                                                                        gtgtTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCAcc                           <  7:296259/135‑1 (MQ=255)
                                                                                                        gtgtTGACCGCCAACAATCCGCTAGCGTTCGAAAAGCAGCTTTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCAcc                           <  1:94326/135‑1 (MQ=255)
                                                                                                         tgtTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCt                          >  5:54007/1‑135 (MQ=255)
                                                                                                                cGCCAACAATCCGCATGCGTGCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCTTGCaaat                   <  6:139093/135‑4 (MQ=255)
                                                                                                                           cGCATGTGTGCGAAACGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCTTGCACCTGCGCACCAAcc        <  7:205129/135‑1 (MQ=255)
                                                                                                                            gaaTGTGTTCGAAACTCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCTTGCACCTGCGCACCAAcc        <  7:199830/132‑1 (MQ=255)
                                                                                                                                gCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCTTGCACCTGCGCACCAACCGccc    >  7:176875/1‑134 (MQ=255)
                                                                                                                                gCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCTTGCACCTGCGCACCAACCGccc    >  8:359821/1‑134 (MQ=255)
                                                                                                                                 cGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCTTGCACCTGCGCACCAACCGCCCGt  <  4:184773/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                      
GCCCCGAGAACCTGATGCTGCTCAAGGAGGACTACCCCTCCCTGAAGATCGTCATGCTCGAACAGAACTACCGTTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGTTGATCGCCAACAATCCGCATGCGTTCGAAAAGCAGCTGTGGAGCGAAATGGGCGTGGGCGACGAGATCCGCGTGATCCGCTGCAAGAACGAGGAAGCCGAGGCCGAACGCGTGGCCATGGAAATCCTCACCTTGCACCTGCGCACCAACCGCCCGT  >  KT2440/6005744‑6006007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑