Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,021,514 T→G 33.9% S60R (AGC→CGC)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,021,5140TG33.9% 12.8 / 6.0 36S60R (AGC→CGC) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base T (11/12);  new base G (6/7);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

CATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACAC  >  KT2440/6021380‑6021648
                                                                                                                                      |                                                                                                                                      
cATGCCTATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                                        <  5:352050/135‑1 (MQ=255)
cATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                                        <  5:24786/135‑1 (MQ=255)
cATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                                        <  5:233419/135‑1 (MQ=255)
     cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                                        >  7:310985/1‑130 (MQ=255)
     cGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCt                                                                                                                                        <  8:310985/130‑1 (MQ=255)
      gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCACCCCAACCTGCCAGGCGGCTGGCCCgg                                                                                                                                 >  3:149905/1‑136 (MQ=255)
      gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTCGccccg                                                                                                                                 >  3:148211/1‑133 (MQ=255)
               ccGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGcc                                                                                                                         <  5:323504/135‑1 (MQ=255)
                  cagcagcagCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGcc                                                                                                                     <  4:295586/136‑1 (MQ=255)
                           ccATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCCGTAcccc                                                                                                             >  6:120363/1‑135 (MQ=255)
                             aTAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCTGGCGCCCGCCACTACGCCCa                                                                                                           >  2:254043/1‑135 (MQ=255)
                                  gggTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACCGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCg                                                                                                     <  3:54541/136‑1 (MQ=255)
                                       aCTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCTGGCGCCCGCCCGTAAGCCCCACCCCGGcct                                                                                                 >  8:263006/1‑133 (MQ=255)
                                        cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGCCAGGCTGGCGCCGGCCCGGACGCCCCACCCCCGccaa                                                                                                >  7:330673/1‑132 (MQ=255)
                                        cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCTGGCGCCCGCCAGTACGCCCCACCCCCGCCTTg                                                                                               >  4:78188/1‑136 (MQ=255)
                                        cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCGCCCGCCCGGACGCCCAccgcccgccat                                                                                                >  6:166454/1‑132 (MQ=255)
                                                cagcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCACCCCAACCTGCCAGGCGGCTGGCCCGGCAGGCGCCCGCCCGACCGCCCAACGCCCGCATTcccgcccg                                                                                        >  4:231975/1‑133 (MQ=255)
                                                cagcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCCGCATTGCCGCCAt                                                                                        >  2:19575/1‑135 (MQ=255)
                                                   cgcgAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCACCGAAGGTACGCCAACCCAACCTGCCAGGCGGGGGGCCCGGCTGGCGCCCGCCCGGACGCCCCACCCCCGCCATgccgcccgcac                                                                                     >  5:283777/1‑130 (MQ=255)
                                                              cAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCACCCTGCCAGGCGGCTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGa                                                                          <  7:311309/135‑1 (MQ=255)
                                                                   taCAGCGCCCCGCCCCCCAGGCACAGGCGAATCGCCGAAGGTACGCCAACCCACCCTGCCAGGGGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTg                                                                     <  7:41014/134‑1 (MQ=255)
                                                                         gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGGCCGGCGGGCGCCCGCCCGTACGCCCACCCCCCGCCGTGGCGCCCGCACGCCCAGGGCGGAGCGGCCGTAg                                                               >  1:324271/1‑135 (MQ=255)
                                                                         gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGCGCCCGCCCGTACGCCCAACCCCCGCATTGCCCCCAACACCCCCCAGGCGCAGCTGCCGTAg                                                               >  7:357931/1‑135 (MQ=255)
                                                                            ccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGcccc                                                           <  3:158673/136‑1 (MQ=255)
                                                                               ccccACCGCCACCGGCGAATCGCCGAAGGTACGCCAACCCAACCTGCCCGGCGGCGGGCCTGGCTGGCGCCGGCCTGAAGGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAgg                                                        <  3:78188/136‑1 (MQ=255)
                                                                                      ggcacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCa                                                  <  7:263006/135‑1 (MQ=255)
                                                                                       gcacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGCGCCCGCCCCAACCCCCCACCCCGGCCTTTCCCCCCACACCCCGCAGGGGTAGCAGCCCTAGCCCCCCGCCTcccac                                                >  4:2930/1‑133 (MQ=255)
                                                                                           cggcGCCTCGCCCACGGCCCCCCACCCCACCCGCCCCGGCGGCGGCCCGGGCGGGCGCCGGCCCGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgcgc                                             <  1:302689/134‑1 (MQ=255)
                                                                                                           gTACGCCAACCCACCCTGCCCGGCGGCTGGCCAGGCTGGCGCGGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                            <  4:134131/136‑1 (MQ=255)
                                                                                                             aCGCCAACCCACCCTCCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATg                           <  8:74377/135‑1 (MQ=255)
                                                                                                                  aacccaaccTGCCAGGCGGCTGGCCACGCTGGCGCCCGCCCGTACGCCCCACGCCGGCATTGCCCCCCTAACGCCGCTGGCGCAGCTGCCGGTGCCCCACGCCTCCCGGCGCAGGGCCCCCAACGGCCTGCTCAc                      >  8:65167/1‑135 (MQ=255)
                                                                                                                      caaccTGCCAGGCGGCTGGCCCCGCTGGCGCCGGCCAGGTCCCCCAACCCCCGCCTTTCCGCCAACACGCCCATGGCGAAGCTGCCGTAGCCCCCCGCCGCCCCGCGCCCGGCCACCCGCCGCCAGCTCACGccc                  >  8:229215/1‑134 (MQ=255)
                                                                                                                       aaccTGCCAGGCGGCTGGCCCGGCTGGCGCCCGCCAGTACCCCCAACCCCGGCCATGCCGCCCAAACCCCGATTGCGAACCAGACCTAGCCCCCCGCCGCCACGCGCAGGGGCACCCCCGGCCTGGACACCCCAAg                >  4:284385/1‑136 (MQ=255)
                                                                                                                       aaccTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  4:309706/136‑1 (MQ=255)
                                                                                                                        ccgcgCCCGGCGGCGGGCCGGGCGGGCGCCGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  2:367726/131‑1 (MQ=255)
                                                                                                                        cccggCCTGGCGGCTGGTCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  1:316081/131‑1 (MQ=255)
                                                                                                                        cccTGCCCGCCGGCGGGCACGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  7:191150/134‑1 (MQ=255)
                                                                                                                        accTGCCCGGCGGCGGGCCAGGCTGGCGCCGGCATGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  2:140229/135‑1 (MQ=255)
                                                                                                                               aGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGc         <  8:357931/135‑1 (MQ=255)
                                                                                                                                  gggcGGGCCGGGCGGGCGCCGCCCCGTACCCCCAACGCCGGCATTGCCGCCAGCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg      <  2:157087/134‑1 (MQ=255)
                                                                                                                                     cTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGacac  <  4:297271/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CATGCCGATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACAC  >  KT2440/6021380‑6021648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑