Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,021,519 A→C 49.2% L58R (CTG→CGG)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,021,5190AC49.2% 1.6 / 13.6 36L58R (CTG→CGG) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base A (3/14);  new base C (13/2);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 2.44e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

GATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACAC  >  KT2440/6021386‑6021648
                                                                                                                                     |                                                                                                                                 
gATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCACCCCAACCTGCCAGGCGGCTGGCCCgg                                                                                                                                 >  3:149905/1‑136 (MQ=255)
         ccGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGcc                                                                                                                         <  5:323504/135‑1 (MQ=255)
            cagcagcagCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGcc                                                                                                                     <  4:295586/136‑1 (MQ=255)
                     ccATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCCGTAcccc                                                                                                             >  6:120363/1‑135 (MQ=255)
                       aTAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCTGGCGCCCGCCACTACGCCCa                                                                                                           >  2:254043/1‑135 (MQ=255)
                            gggTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACCGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCg                                                                                                     <  3:54541/136‑1 (MQ=255)
                                 aCTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCCGCTGGCGCCCGCCCGTAAGCCCCACCCCGGcct                                                                                                 >  8:263006/1‑133 (MQ=255)
                                  cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGCCAGGCTGGCGCCGGCCCGGACGCCCCACCCCCGccaa                                                                                                >  7:330673/1‑132 (MQ=255)
                                  cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCTGGCGCCCGCCAGTACGCCCCACCCCCGCCTTg                                                                                               >  4:78188/1‑136 (MQ=255)
                                  cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCGCCCGCCCGGACGCCCAccgcccgccat                                                                                                >  6:166454/1‑132 (MQ=255)
                                          cagcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCACCCCAACCTGCCAGGCGGCTGGCCCGGCAGGCGCCCGCCCGACCGCCCAACGCCCGCATTcccgcccg                                                                                        >  4:231975/1‑133 (MQ=255)
                                          cagcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCCGCATTGCCGCCAt                                                                                        >  2:19575/1‑135 (MQ=255)
                                             cgcgAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCACCGAAGGTACGCCAACCCAACCTGCCAGGCGGGGGGCCCGGCTGGCGCCCGCCCGGACGCCCCACCCCCGCCATgccgcccgcac                                                                                     >  5:283777/1‑130 (MQ=255)
                                                        cAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCACCCTGCCAGGCGGCTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGa                                                                          <  7:311309/135‑1 (MQ=255)
                                                             taCAGCGCCCCGCCCCCCAGGCACAGGCGAATCGCCGAAGGTACGCCAACCCACCCTGCCAGGGGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTg                                                                     <  7:41014/134‑1 (MQ=255)
                                                                   gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGGCCGGCGGGCGCCCGCCCGTACGCCCACCCCCCGCCGTGGCGCCCGCACGCCCAGGGCGGAGCGGCCGTAg                                                               >  1:324271/1‑135 (MQ=255)
                                                                   gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGCGCCCGCCCGTACGCCCAACCCCCGCATTGCCCCCAACACCCCCCAGGCGCAGCTGCCGTAg                                                               >  7:357931/1‑135 (MQ=255)
                                                                      ccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGcccc                                                           <  3:158673/136‑1 (MQ=255)
                                                                         ccccACCGCCACCGGCGAATCGCCGAAGGTACGCCAACCCAACCTGCCCGGCGGCGGGCCTGGCTGGCGCCGGCCTGAAGGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAgg                                                        <  3:78188/136‑1 (MQ=255)
                                                                                ggcacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCa                                                  <  7:263006/135‑1 (MQ=255)
                                                                                 gcacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGCGCCCGCCCCAACCCCCCACCCCGGCCTTTCCCCCCACACCCCGCAGGGGTAGCAGCCCTAGCCCCCCGCCTcccac                                                >  4:2930/1‑133 (MQ=255)
                                                                                     cggcGCCTCGCCCACGGCCCCCCACCCCACCCGCCCCGGCGGCGGCCCGGGCGGGCGCCGGCCCGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgcgc                                             <  1:302689/134‑1 (MQ=255)
                                                                                                     gTACGCCAACCCACCCTGCCCGGCGGCTGGCCAGGCTGGCGCGGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                            <  4:134131/136‑1 (MQ=255)
                                                                                                       aCGCCAACCCACCCTCCCAGGCGGCGGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATg                           <  8:74377/135‑1 (MQ=255)
                                                                                                            aacccaaccTGCCAGGCGGCTGGCCACGCTGGCGCCCGCCCGTACGCCCCACGCCGGCATTGCCCCCCTAACGCCGCTGGCGCAGCTGCCGGTGCCCCACGCCTCCCGGCGCAGGGCCCCCAACGGCCTGCTCAc                      >  8:65167/1‑135 (MQ=255)
                                                                                                                caaccTGCCAGGCGGCTGGCCCCGCTGGCGCCGGCCAGGTCCCCCAACCCCCGCCTTTCCGCCAACACGCCCATGGCGAAGCTGCCGTAGCCCCCCGCCGCCCCGCGCCCGGCCACCCGCCGCCAGCTCACGccc                  >  8:229215/1‑134 (MQ=255)
                                                                                                                 aaccTGCCAGGCGGCTGGCCCGGCTGGCGCCCGCCAGTACCCCCAACCCCGGCCATGCCGCCCAAACCCCGATTGCGAACCAGACCTAGCCCCCCGCCGCCACGCGCAGGGGCACCCCCGGCCTGGACACCCCAAg                >  4:284385/1‑136 (MQ=255)
                                                                                                                 aaccTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  4:309706/136‑1 (MQ=255)
                                                                                                                  ccgcgCCCGGCGGCGGGCCGGGCGGGCGCCGGCCAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  2:367726/131‑1 (MQ=255)
                                                                                                                  cccggCCTGGCGGCTGGTCAGGCGGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  1:316081/131‑1 (MQ=255)
                                                                                                                  cccTGCCCGCCGGCGGGCACGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  7:191150/134‑1 (MQ=255)
                                                                                                                  accTGCCCGGCGGCGGGCCAGGCTGGCGCCGGCATGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                <  2:140229/135‑1 (MQ=255)
                                                                                                                         aGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGc         <  8:357931/135‑1 (MQ=255)
                                                                                                                            gggggggCCCGGCGGGCGCGGCCCTGAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg      <  2:324271/130‑1 (MQ=255)
                                                                                                                            gggcGGGCCGGGCGGGCGCCGCCCCGTACCCCCAACGCCGGCATTGCCGCCAGCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg      <  2:157087/134‑1 (MQ=255)
                                                                                                                               cTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGacac  <  4:297271/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
GATCATCAGCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACAC  >  KT2440/6021386‑6021648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑