Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,048,049 T→A 6.5% G186G (GGT→GGA)  tolQ_3 → Tol‑Pal system protein TolQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,048,0490TA 6.5% 214.8 / 4.5 76G186G (GGT→GGA) tolQ_3Tol‑Pal system protein TolQ
Reads supporting (aligned to +/- strand):  ref base T (26/45);  new base A (2/3);  total (28/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

CCCAGCTGAAGAAGTCCACCACCCTGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGTTCGGTA  >  KT2440/6047916‑6048173
                                                                                                                                     |                                                                                                                            
cccAGCTGAAGAAGTCCACCACCCTGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTACCACTCGCGAAAAAGAAGGt                                                                                                                              >  2:170266/1‑134 (MQ=255)
        aagaagTGCACCAACCTGAAAGAAGCCAGCGAGGTCACCATCAAGGATGGCACCCTGGCCCCCACCCTGGTCAACGACGACCTCGAAGAGATGACTCTTTCGGCTAACGCGCGCGAAAATGTAGTTATCATGGAg                                                                                                                     >  6:211037/1‑135 (MQ=255)
               ccaccaCCCTGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCa                                                                                                              <  1:112470/135‑1 (MQ=255)
               ccaccaCCCTGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTATTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCa                                                                                                              <  6:18753/135‑1 (MQ=255)
                caccacCCTGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGTCCCACACCCTTGTCCACTACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGAGAAAAAGAAGGTATCAAGGAGCGCGTCAGc                                                                                                            <  4:220662/136‑1 (MQ=255)
                  ccaccCTGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCt                                                                                                           >  2:202448/1‑135 (MQ=255)
                  ccaccCTGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCt                                                                                                           >  6:211906/1‑135 (MQ=255)
                        tGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTc                                                                                                     <  2:193379/135‑1 (MQ=255)
                          aaaGAAGCCAGCGAGGTCACCAACAAGGACGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCt                                                                                                    <  3:294216/134‑1 (MQ=255)
                           aagaagCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCt                                                                                                    >  1:101025/1‑133 (MQ=255)
                           aagaagCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCt                                                                                                    >  1:234404/1‑133 (MQ=255)
                           aagaagCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCt                                                                                                    >  1:197850/1‑133 (MQ=255)
                           aagaagCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAACAGATGCGTCTTTCGGCTAACGCTCGCGAAAAACAAGGTATAAAGGAGCGCGTCAGCTACCGTCTg                                                                                                   >  4:201625/1‑134 (MQ=255)
                             gaagCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAgc                                                                                               <  3:95871/136‑1 (MQ=255)
                             gaagCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAg                                                                                                >  7:254302/1‑135 (MQ=255)
                              acgCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAgc                                                                                               <  2:130912/133‑1 (MQ=255)
                              acgCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGGCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAgc                                                                                               <  1:187043/133‑1 (MQ=255)
                              aagCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAgc                                                                                               <  5:158856/135‑1 (MQ=255)
                              aagCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAgc                                                                                               <  2:101025/135‑1 (MQ=255)
                                  cAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCt                                                                                           >  8:12094/1‑135 (MQ=255)
                                  cAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTg                                                                                          >  3:224593/1‑136 (MQ=255)
                                   aGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTg                                                                                          >  7:277016/1‑135 (MQ=255)
                                       aGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGc                                                                                      >  1:329045/1‑135 (MQ=255)
                                        ggTCACAAACAAGGACGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGATAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCAt                                                                                    <  4:266871/136‑1 (MQ=255)
                                         gTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCAt                                                                                    <  2:261783/135‑1 (MQ=255)
                                         gTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCAt                                                                                    <  8:192075/135‑1 (MQ=255)
                                         atCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCAt                                                                                    <  8:304250/134‑1 (MQ=255)
                                               accacggaaggCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCg                                                                             <  3:19801/131‑1 (MQ=255)
                                                accaggaaggCACCCTGGCCCACACCCTCGGCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCg                                                                             <  5:341224/132‑1 (MQ=255)
                                                       aggCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGAATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCAc                                                                      >  4:108872/1‑135 (MQ=255)
                                                       aggCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGAATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCAc                                                                      >  4:103514/1‑135 (MQ=255)
                                                           aCCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCa                                                                    >  8:358370/1‑133 (MQ=255)
                                                             ccggtcccACACCCGGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCGAACGCGCGCTAAAAAGAAGGTCTCCAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCAGGCCAGCGGCCGCACCATGag                                                                <  2:244850/130‑1 (MQ=255)
                                                             ccTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGag                                                                <  8:164234/135‑1 (MQ=255)
                                                               tGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGagca                                                              >  1:225227/1‑135 (MQ=255)
                                                                  cccACACCCTGGTCCACGACGCCCTCGAAGAGCTGCGTCTTTCGGCTAACGCTCGCGAAAAAGCAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCCCCATGAGCAGCg                                                           >  1:190430/1‑135 (MQ=255)
                                                                   ccACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCCCCATGAGCAGCgg                                                          >  5:118437/1‑135 (MQ=255)
                                                                     acacCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGGCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCAc                                                       <  4:224593/136‑1 (MQ=255)
                                                                       acCCTGCTCCACGACTCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCAcc                                                      <  1:122046/135‑1 (MQ=255)
                                                                              tCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGGATGCCAGCGGCCGCCCCATGAGCCCCGGCCCCGGCGTca                                               >  2:160364/1‑134 (MQ=255)
                                                                              tCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCt                                              >  3:59769/1‑136 (MQ=255)
                                                                               ccACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCa                                                                       >  6:346814/1‑110 (MQ=255)
                                                                               ccACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCa                                                                       <  5:346814/110‑1 (MQ=255)
                                                                               ccACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCt                                              >  6:179095/1‑135 (MQ=255)
                                                                                cACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAgc                                                                                               >  5:182131/1‑85 (MQ=255)
                                                                                cACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAgc                                                                                               <  6:182131/85‑1 (MQ=255)
                                                                                   gccgCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGCGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCa                                          <  6:9587/133‑1 (MQ=255)
                                                                                   gacgCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCa                                          <  2:329045/135‑1 (MQ=255)
                                                                                      gCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAAcc                                       >  8:23632/1‑135 (MQ=255)
                                                                                          tCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCg                                   >  2:124131/1‑135 (MQ=255)
                                                                                          tCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCAGCGTCCTCGCAACCATCg                                   <  6:31362/135‑1 (MQ=255)
                                                                                                            cGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGt                    <  8:19250/132‑1 (MQ=255)
                                                                                                            cGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGt                    <  7:358370/132‑1 (MQ=255)
                                                                                                             ggCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTtcgtc               <  3:34041/136‑1 (MQ=255)
                                                                                                             ggCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTtcgtc               <  3:259854/136‑1 (MQ=255)
                                                                                                             ggCTAACGCTCGCGAAAAAGAAGGAATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTtcgtc               <  3:103514/136‑1 (MQ=255)
                                                                                                             ggCTAACGCTCGCGAAAAAGAAGGAATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTtcgtc               <  3:108872/136‑1 (MQ=255)
                                                                                                              gCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTtcgtc               <  7:240540/135‑1 (MQ=255)
                                                                                                              gCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTtcgtc               <  7:192979/135‑1 (MQ=255)
                                                                                                                  aCGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCt          <  3:335491/136‑1 (MQ=255)
                                                                                                                  aCGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCt          <  4:338308/136‑1 (MQ=255)
                                                                                                                   cGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGc            <  1:202448/133‑1 (MQ=255)
                                                                                                                   cGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCt          <  7:12094/135‑1 (MQ=255)
                                                                                                                    gCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGt        <  4:283990/136‑1 (MQ=255)
                                                                                                                     cTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCa                                                                                >  3:323955/1‑63 (MQ=255)
                                                                                                                     cTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCa                                                                                <  4:323955/63‑1 (MQ=255)
                                                                                                                     cTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCg                                                     <  3:216694/90‑1 (MQ=255)
                                                                                                                     cTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCg                                                     >  4:216694/1‑90 (MQ=255)
                                                                                                                     cTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGt        <  6:234058/135‑1 (MQ=255)
                                                                                                                     cTCGCGAAAAAGAAGGAATCACGGAGCGCGTCAGCTGCCGTCTGGAGCGCCTGGTGAATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGt        <  6:45827/135‑1 (MQ=255)
                                                                                                                      tCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGtt       <  5:179095/135‑1 (MQ=255)
                                                                                                                       cgcgAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGTTCg     <  4:336724/136‑1 (MQ=255)
                                                                                                                        gcgAAAATGAAGGTATCATGGAGCGCGTCAGATTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGTTCg     <  5:23636/135‑1 (MQ=255)
                                                                                                                        gcgAAAAAGAAGGTATCATGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGTTCg     <  1:319900/135‑1 (MQ=255)
                                                                                                                         cgAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGTTCg     <  6:318757/134‑1 (MQ=255)
                                                                                                                         cgAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGTTCg     <  6:216140/134‑1 (MQ=255)
                                                                                                                           aaaaaGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGTTCGGTa  >  6:326896/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                            
CCCAGCTGAAGAAGTCCACCACCCTGAAAGAAGCCAGCGAGGTCACCAACAAGGAAGGCACCCTGGCCCACACCCTGGTCCACGACGCCCTCGAAGAGATGCGTCTTTCGGCTAACGCTCGCGAAAAAGAAGGTATCAAGGAGCGCGTCAGCTTCCGTCTGGAGCGCCTGGTGCATGCCAGCGGCCGCACCATGAGCAGCGGCACCGGCGTCCTCGCAACCATCGGCTCCACCGCGCCGTTCGTCGGCCTGTTCGGTA  >  KT2440/6047916‑6048173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑