Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,073,133 A→T 5.9% intergenic (‑73/‑289) abaF_2 ← / → CJ019_05688 Fosfomycin resistance protein AbaF/putative acyl‑CoA thioester hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,073,1330AT 5.9% 217.6 / 3.7 68intergenic (‑73/‑289)abaF_2/CJ019_05688Fosfomycin resistance protein AbaF/putative acyl‑CoA thioester hydrolase
Reads supporting (aligned to +/- strand):  ref base A (24/40);  new base T (2/2);  total (26/42)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

TGTAGTCGCTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATG  >  KT2440/6073000‑6073262
                                                                                                                                     |                                                                                                                                 
tGTAGTCGCTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATa                                                                                                                                   >  7:44554/1‑134 (MQ=255)
tGTAGTCGCTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATa                                                                                                                                   >  7:236926/1‑134 (MQ=255)
tGTAGTCGCTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATa                                                                                                                                   >  5:244180/1‑134 (MQ=255)
tGTAGTCGCTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCGGGGCACGGAAGTCATAGCTCGTTGTTCTCTTTGTATGGCATTCAGGCCCCGTGGCGCGCGGCATCTGGAATGGAGCCAGGCGTAAATa                                                                                                                                   >  2:352331/1‑134 (MQ=255)
tGTAGTCGCTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACAGCTGGGCACGGAATTCCTCGCTCGTTGTTCTGGTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATa                                                                                                                                   >  2:197075/1‑134 (MQ=255)
tGTAGTCGCTGCGGGTCAGCGGCCGCGAGCGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCACGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATa                                                                                                                                   >  7:215226/1‑134 (MQ=255)
  tAGTCGCTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATa                                                                                                                                   <  2:274911/132‑1 (MQ=255)
  tAGTCGCTGCGGGTCAGCGGCCGCCAGGGCTGCTAGATTCTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCGTCTGGATTGGAGACAGGAGAAGATAGc                                                                                                                                 >  7:25845/1‑134 (MQ=255)
        cTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGcc                                                                                                                         >  3:149507/1‑136 (MQ=255)
          gCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGc                                                                                                                          >  8:111453/1‑133 (MQ=255)
          gCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCg                                                                                                                        <  7:111453/135‑1 (MQ=255)
          gCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCGTTGTAGGGCCTGCAGGCCGCTTGGCGCGCGGCTTCGGGAGTGGAGCCAGGCGAAGCTAGCAAATTGcc                                                                                                                         >  3:311408/1‑134 (MQ=255)
          gCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGGTGTTGTCTTTGTAGGGCATGCCGGCCGCTTGGCGCGCGGCGTCTGGATTGGTGACCGGCGAAGATAGCAAATTGc                                                                                                                          >  1:272399/1‑133 (MQ=255)
          gCGGGTCAGCGGCCGCGAGGGCGGCGCGATTCTGCTGGGCACGGAAGTGAGAGCTCGTTGTTGTCGTTGTTGGGGCTGCAGGCCGCGTGGCGCGGGGGGTCGGGGTTGGGGCCCGGCGCATATAGGAAATTGCCg                                                                                                                        >  6:208381/1‑135 (MQ=255)
                     gCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGa                                                                                                             <  1:165114/135‑1 (MQ=255)
                           aGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCg                                                                                                        >  7:265329/1‑134 (MQ=255)
                            gggCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCGTCTGGATTGGAGCCAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGAt                                                                                                      >  5:169265/1‑135 (MQ=255)
                                     gATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCca                                                                                            >  3:135225/1‑136 (MQ=255)
                                     gATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCca                                                                                            <  4:272529/136‑1 (MQ=255)
                                     gATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCca                                                                                            <  4:230738/136‑1 (MQ=255)
                                               ggCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCca                                                                                            <  5:260448/126‑1 (MQ=255)
                                               ggCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCca                                                                                            >  6:260448/1‑126 (MQ=255)
                                                  aCGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATc                                                                                              <  7:169732/121‑1 (MQ=255)
                                                  aCGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATc                                                                                              >  8:169732/1‑121 (MQ=255)
                                                    ggAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGt                                                                               >  7:254935/1‑134 (MQ=255)
                                                    ggAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGt                                                                               >  2:149261/1‑134 (MQ=255)
                                                    ggAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGt                                                                               >  8:192899/1‑134 (MQ=255)
                                                    ggAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCGTCGGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGt                                                                               >  2:339945/1‑134 (MQ=255)
                                                    ggAAGTCATCGCTCGGTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGCTAGCAAATTGCCGAGCTACGCCGCAAGCGATACAAAATGCACACAGAAATTCGTg                                                                              >  4:18028/1‑135 (MQ=255)
                                                       aGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAAt                                                                           <  5:119248/135‑1 (MQ=255)
                                                       aGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAAt                                                                           >  6:113899/1‑135 (MQ=255)
                                                           aTCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATgcgc                                                                       <  7:345013/135‑1 (MQ=255)
                                                                    tgttCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGc                                                              <  8:215226/135‑1 (MQ=255)
                                                                    tgttCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGc                                                              <  8:254935/135‑1 (MQ=255)
                                                                          ctTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTTCCGAGCTAACCCCAAAGCGATACAAAATCCACACAGATATTCGTGAAATCGCGGTAGTCGCTGTCCGt                                                       >  4:112164/1‑136 (MQ=255)
                                                                            ttGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGATACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGcc                                                         <  2:329776/132‑1 (MQ=255)
                                                                            ttGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGcc                                                         <  8:47857/132‑1 (MQ=255)
                                                                             tGTAGGGCATGCAGGGCGATTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGGCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGt                                                       <  4:320560/133‑1 (MQ=255)
                                                                             tGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATTGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTa                                                     >  1:271485/1‑135 (MQ=255)
                                                                             tGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATTGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGGTa                                                     >  1:266493/1‑135 (MQ=255)
                                                                             tGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGt                                                       <  4:135225/133‑1 (MQ=255)
                                                                             tGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCAGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATTCGCGGTCGTCGCTGGCCGTTa                                                     >  6:343627/1‑135 (MQ=255)
                                                                              gTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATTGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGt                                                       <  2:266493/132‑1 (MQ=255)
                                                                              gTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATTGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGt                                                       <  2:271485/132‑1 (MQ=255)
                                                                              gTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGtt                                                      <  2:305958/133‑1 (MQ=255)
                                                                              gTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGt                                                       <  1:204773/132‑1 (MQ=255)
                                                                              gTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGt                                                       <  1:352331/132‑1 (MQ=255)
                                                                              gTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGt                                                       <  6:244180/132‑1 (MQ=255)
                                                                               tAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTAt                                                    <  6:341420/134‑1 (MQ=255)
                                                                               tAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTAt                                                    <  7:63738/134‑1 (MQ=255)
                                                                               tAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTAt                                                    <  5:113899/134‑1 (MQ=255)
                                                                               tAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTAt                                                    <  2:244579/134‑1 (MQ=255)
                                                                               tAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTAt                                                    <  6:131576/134‑1 (MQ=255)
                                                                               tAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTAt                                                    <  2:293251/134‑1 (MQ=255)
                                                                                aGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGc                                                  <  8:236926/135‑1 (MQ=255)
                                                                                     aTGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCtat                                             <  6:157002/135‑1 (MQ=255)
                                                                                        cAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTAt                                                    >  3:43331/1‑125 (MQ=255)
                                                                                        cAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTAt                                                    <  4:43331/125‑1 (MQ=255)
                                                                                        cAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCtatata                                          >  7:258916/1‑135 (MQ=255)
                                                                                           gCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTg                                       <  8:203551/135‑1 (MQ=255)
                                                                                               cTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGaa                                  <  4:185596/136‑1 (MQ=255)
                                                                                                ttGGCGCGCGGCTTCTGGATTGGAGTCAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGAGATACTAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGaa                                  <  2:75924/135‑1 (MQ=255)
                                                                                                ttGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGaa                                  <  1:329046/135‑1 (MQ=255)
                                                                                                ttGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGaa                                  <  5:145707/135‑1 (MQ=255)
                                                                                                ttGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGaa                                  >  2:219715/1‑135 (MQ=255)
                                                                                                ttGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGaa                                  <  7:192899/135‑1 (MQ=255)
                                                                                                          gCTTCTGGATTGGAGACAGGCGAAGATAGCTAATTGCCGATCTACGCCGAAAGCGATACAAAATCGAGACAGATATTCGTGAATGCTCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGAtt                        <  8:25845/135‑1 (MQ=255)
                                                                                                           cTTCTGGATTGGAGACAGGCGAAGATATCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGAGAATCGTAGGATTa                       <  1:27737/135‑1 (MQ=255)
                                                                                                                  aTTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCt                <  1:589/135‑1 (MQ=255)
                                                                                                                   ttGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTc               <  8:258916/135‑1 (MQ=255)
                                                                                                                      gagaCAGTCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCt             <  5:354537/134‑1 (MQ=255)
                                                                                                                       agaCAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTaa           >  7:33380/1‑135 (MQ=255)
                                                                                                                             gCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTgtg     <  8:33380/135‑3 (MQ=255)
                                                                                                                              cGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGa    >  5:178676/1‑135 (MQ=255)
                                                                                                                                  gATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGAtg  <  8:357379/133‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
TGTAGTCGCTGCGGGTCAGCGGCCGCGAGGGCTGCTCGATACTGCTGGGCACGGAAGTCATCGCTCGTTGTTCTCTTTGTAGGGCATGCAGGCCGCTTGGCGCGCGGCTTCTGGATTGGAGACAGGCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATG  >  KT2440/6073000‑6073262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: