Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,139,542 T→G 16.9% N2T (AAC→ACC)  CJ019_05747 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,139,5420TG16.9% 54.1 / 2.3 40N2T (AAC→ACC) CJ019_05747hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/21);  new base G (6/2);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 5.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGCTCCAACGACATGGGCAAGGTGCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTGGCGGGCGG  >  KT2440/6139410‑6139666
                                                                                                                                    |                                                                                                                            
aGGGCTCCAACGACATGGGCAAGGTGCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTGATCGAAGTGAGGGttga                                                                                                                           >  3:90909/1‑134 (MQ=255)
 gggCTCCAACGACATGGGCAAGGTGCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGGTCATCGAAGTGGGGGttg                                                                                                                            >  1:81804/1‑133 (MQ=255)
   gCTCCAACGACATGGGCAAGGTGCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAg                                                                                                                        <  4:179936/136‑1 (MQ=255)
     tCCAACGACATGGGCAAGGTGCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTGATAGCAGGGAGGGTTCATAGc                                                                                                                       >  6:229390/1‑135 (MQ=255)
     tCCAACGACATGGGCAAGGTGCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGGTGATCGAAGTGAGGGGTGATaag                                                                                                                       >  5:4556/1‑133 (MQ=255)
              aTGGGCAAGGTGCGCGGGGAGCAGGACGTCGACGGCGAGCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAAGTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTgg                                                                                                              <  6:67875/135‑1 (MQ=255)
                   cacggggCTGGGGGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACGGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAag                                                                                                         <  1:107061/129‑1 (MQ=255)
                    aaGGTGCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTGATCGAAGTGAGGGTTGATTTCGACAGCTGGGgaa                                                                                                          >  2:151768/1‑133 (MQ=255)
                         gCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAATGAGGTg                                                                                                   >  8:39168/1‑135 (MQ=255)
                         gCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGGTCATAGCGACTCCGGGGAAAGGAGGTg                                                                                                   >  7:315636/1‑135 (MQ=255)
                              gTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGTAGTGAGGGGTCCTTGCGCCGCCCGGGAAAGGAGGGGGGTTa                                                                                              >  2:213466/1‑135 (MQ=255)
                                     ggAAGTAGCCGTCGATCCCACAGATTACCACCAGGCCGATTGCGTAGCGTGACCGCCAGAAACGGGGCGGAGAGCCGTGTTCATCGAAGTGCGGGTTCATAGCGACTCCTGGGAAAGTAGGTGGGTTACTTCGAtg                                                                                      <  4:141285/136‑1 (MQ=255)
                                     ggAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGGTCATAGAAGTGAGGGGTGATAGCGTCGCCTGGGCAAGGAGGTGGGTTACTACGAt                                                                                       >  6:297160/1‑135 (MQ=255)
                                      gAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGGTCATAGCGACTCCTGGGAAAGGAGGGGGGGTTCTTCGAtgc                                                                                     >  4:354911/1‑136 (MQ=255)
                                       aaGTAGACGGCGAACCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGAt                                                                                       <  6:37569/133‑1 (MQ=255)
                                         cagcccGGCGACCCCACCGATGACCACCCGCCCGTGTGCGTCGCGTGACCGCCAGAACCTGGGCGGAGAGCCGTTTTCATCGAAGTGCGGGTTCAACGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGAtgctg                                                                                   <  2:371540/130‑1 (MQ=255)
                                          tAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCGGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGAtgctgc                                                                                  <  5:121788/135‑1 (MQ=255)
                                              cGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATg                                                                             <  4:138392/136‑1 (MQ=255)
                                                gCGATCCCATCGATTACCACCAGGCCTATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGc                                                                           <  3:84731/136‑1 (MQ=255)
                                                 cGACCCCACCGATTACCACCAGGCCGATGGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGc                                                                           <  1:355540/135‑1 (MQ=255)
                                                   aTCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCCTGGAAGTGAGGGGTGATAGGGACGCCTGGGCAAGGAGGTGGGGTTCTTAGATGCTGCAGATGGCAt                                                                         >  6:96065/1‑135 (MQ=255)
                                                   aTCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGTAGTGAGGGTTCATATCGGCTCCTGGGAAAGGAGGTGGGGTACTTAGATGGTGGAGATgggct                                                                         >  1:256797/1‑132 (MQ=255)
                                                    tCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATAGAAGTGAGGGTTGATATCGCCACCGGGGAAAGGAGGTGGGTTTCTTAGAATCTGCAGATGGCCTg                                                                        >  1:208674/1‑135 (MQ=255)
                                                                        ccGATTGCGTAGCGTCAACGCCAGAACCTGGGCGGAGAGCCGTGTTCATCGAAGTGCGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGcat                                                    <  7:338277/135‑1 (MQ=255)
                                                                        ccGATGGCGAAGCGTGACCCCCCAAAACTGGGCGGAGAGCCGTGTTCATCGAAGGGCGGGTTCAACGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGcat                                                    <  6:55332/135‑1 (MQ=255)
                                                                          gATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACt                                                                                                                   >  7:308126/1‑70 (MQ=255)
                                                                          gATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACt                                                                                                                   <  8:308126/70‑1 (MQ=255)
                                                                           aTTGCGTAGCGTTAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCatcatc                                                <  4:156090/136‑1 (MQ=255)
                                                                              gCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTGATCGAAGTGTGGGTTAATAGCGACTCCTGGGTAAGGAGGTGGGTTTCATAGATTCTTCAGAATGCAAGCGGTCCAACATCATCAGCATCATAGa                                              >  5:185443/1‑135 (MQ=255)
                                                                                  cgCGTGAACGCCAGAAAGTGGGCGGTGAGCGGTGTTCATCGAAGTGAGGGGTCATCGCGACTCCTGGGAAAGGAGGGGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTcc                                          <  6:4556/134‑1 (MQ=255)
                                                                                           gCCAGAAACGGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCAAAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTcc                                 <  5:160070/135‑1 (MQ=255)
                                                                                                aaaCTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGTAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATgcgg                           >  4:169812/1‑135 (MQ=255)
                                                                                                 aaCTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTGATAGCGACTCCGGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCt                          >  4:161913/1‑136 (MQ=255)
                                                                                                             agCCGGGTGCATCTAAGTGAGGGTTCATAGCGCCTCCTGGGAACGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCt              <  4:71297/136‑1 (MQ=255)
                                                                                                                        tCGAAGGGCGGGTGCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTggcgggcg   <  3:354911/136‑1 (MQ=255)
                                                                                                                         ctccgTGAGGGGCCATCGCGACTCCTGGGAACGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTggcgggcg   <  1:151768/131‑1 (MQ=255)
                                                                                                                         cGAAGTGAGGGTTCATCGCGACTCCTGGGAACGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTggcgggcg   <  2:81804/135‑1 (MQ=255)
                                                                                                                          gAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTGgcgggcgg  <  4:90909/135‑1 (MQ=255)
                                                                                                                          gAAGTGAGGGTTCATAGCGACTCCTGGGAAACGAGGTGGGTTACTTACATGCTGCAGATGGCATTCGGTCCAACATCATATGCCTCAACGACTCCAACATTTACATGCGCGTCTCCAAAAACTGGTGgcgggcgg  >  2:196534/1‑135 (MQ=255)
                                                                                                                           gctggAGGGTACAATGCGACTCCGGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGGCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTggcgggcg   <  5:297160/129‑1 (MQ=255)
                                                                                                                                   gTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTggc        >  4:316291/1‑120 (MQ=255)
                                                                                                                                   gTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTggc        <  3:316291/120‑1 (MQ=255)
                                                                                                                                    |                                                                                                                            
AGGGCTCCAACGACATGGGCAAGGTGCTCGGTGAGCAGGAAGTAGACGGCGATCCCACCGATTACCACCAGGCCGATTGCGTAGCGTGAACGCCAGAAACTGGGCGGAGAGCCGTGTTCATCGAAGTGAGGGTTCATAGCGACTCCTGGGAAAGGAGGTGGGTTACTTCGATGCTGCAGATGGCATGCGGTCCATCATCATCTGCATCATCGACTCCATCATGTCCATGCGCTTCTCCATCATCTGGTGGCGGGCGG  >  KT2440/6139410‑6139666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑