Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 444,535 A→C 23.5% L130V (TTG→GTG)  hdfR_1 ← HTH‑type transcriptional regulator HdfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440444,5350AC23.5% 46.5 / 9.8 47L130V (TTG→GTG) hdfR_1HTH‑type transcriptional regulator HdfR
Reads supporting (aligned to +/- strand):  ref base A (22/13);  new base C (2/10);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 7.84e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

TGGGTATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGCGACTGGCCCTT  >  KT2440/444405‑444667
                                                                                                                                  |                                                                                                                                    
tgggtATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCg                                                                                                                                 <  3:222840/136‑1 (MQ=255)
 gggtATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAACCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCg                                                                                                                                 <  1:9855/135‑1 (MQ=255)
 gggtATCGGGTATGACCAGTTGCAGCGATTCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCg                                                                                                                                 <  2:191455/135‑1 (MQ=255)
       cGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACt                                                                                                                           >  1:106565/1‑135 (MQ=255)
       cGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACt                                                                                                                           >  5:21410/1‑135 (MQ=255)
            aTGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGGACTCCAACCCCGACAAcgcgga                                                                                                                       >  1:186260/1‑132 (MQ=255)
            aTGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTaa                                                                                                                      >  7:112469/1‑135 (MQ=255)
               aCCAGTTGCAGCTCATCTTCGTAAAACAGCAGGCCATGCAGCGCGGGGTGGCGCGGCGGAAAATAGGTGATGCCGTTGTCCTGGTTGCGGTTGAGCCGGCGCGGTTCGATCTCCAACCCCGACAAGTCGTAAAt                                                                                                                    <  8:112469/134‑1 (MQ=255)
                 cAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTAGAGCAGGCGCCGTTCGATCTCAAACCCCGACAACTCGTAAATCt                                                                                                                  >  6:225835/1‑134 (MQ=255)
                 cAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGAGGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTg                                                                                                                 >  8:144153/1‑135 (MQ=255)
                  aGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCGGGCTGGCGCGGCGGAAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCTACAACTCGTAAATCTGc                                                                                                                <  5:128992/135‑1 (MQ=255)
                   gTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCAc                                                                                                              >  4:144619/1‑136 (MQ=255)
                    tggCAGCTCATCTTCGTACACCAGCCGCCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGGTGAGGCCGTTGGCCTGGTTGCCGTTGAGCTGGCGCCGTTCGATCTCCAACCCCGACAACTCTTAAATCTGCACg                                                                                                             <  4:190247/134‑1 (MQ=255)
                      gctgCTCATGTTGGTACAACAGCCGGCCATGCAGCCGGGGGGGGCGCGGCGTCAAATCGGTTTGGCGGATGGCCTGGGTGCGGTGGAGCAGGGGCGGTTCGATCTCCAACCCCGACAACTGTTAAATCTGCACg                                                                                                             <  8:261890/131‑1 (MQ=255)
                       cAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGa                                                                                                            >  5:324797/1‑134 (MQ=255)
                       cAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGa                                                                                                            >  5:130895/1‑134 (MQ=255)
                       cAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCACACCCCGACAACTCGTAAATCTGAACGAc                                                                                                           >  6:246414/1‑135 (MQ=255)
                       cAGCTCATCTTCGTACAACAGCAGGCAATTCATCCCTGGCTGTCGCGGAGGAAAATAGCTGATGACGATGTCAAGGTTGCAGTTGAGAAGGCGCCGTTCGATCTCAAACCACGACAACTCCTAAATCTGCACGAc                                                                                                           >  3:345062/1‑135 (MQ=255)
                                 tctcacaacaGCCGCCCAGCCAGCCGGGGCTGGCGGGTCGACAAAACGCTGCTGCCGTTGCCCAGGTTGCGGTTGACCCGGCGCCGTTCTATCACCACCCCCCAAAAAGCTTAAATCTCCACGACCAGGTGAGGtt                                                                                                <  4:243332/132‑1 (MQ=255)
                                 tCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGt                                                                                                 >  1:8961/1‑135 (MQ=255)
                                    tACAACCGCCGCCCATGCAGCCCTGGGTGGCGGGGCGTCAAATAGCTGATGCCTAGGTCCTGGTTGCCTTTGAGCAGGCGCCGTTCGATCTCCAACCCCCACAACTCTTAAATCTGCACGACCAGGTGAGGTTGa                                                                                              <  6:130895/135‑1 (MQ=255)
                                     acaacaGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGc                                                                                            >  4:143198/1‑136 (MQ=255)
                                          agcagGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTgc                                                                                       >  4:294007/1‑136 (MQ=255)
                                               gCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGAAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAg                                                                                   >  8:182983/1‑135 (MQ=255)
                                                       gCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAag                                                                          >  3:264102/1‑136 (MQ=255)
                                                             gctggcGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTg                                                                    >  3:22537/1‑136 (MQ=255)
                                                                 gcgcGGCGGAAAATAGCTGATGCCGATGGCCCGGTGGCGGTTCCGCCGGCGCGGTTCCATCACCACCCCCCAAAACTCGTAAACCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCgg                                                                 <  8:150368/135‑1 (MQ=255)
                                                                     ggcggcAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGCTCTCCACCCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGAt                                                              >  4:31424/1‑134 (MQ=255)
                                                                     ggcggcAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGa                                                               >  7:91786/1‑133 (MQ=255)
                                                                      gcgtcAAATAGGTGAGGCCTCTGGCCAGGTGGCCGTTGAGCCGGCGCCGTTCGAGCTCCACCCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAg                                                           <  4:279105/136‑1 (MQ=255)
                                                                              tcgCTGATGCCGAGGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGAAAATCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                   <  3:178003/134‑1 (MQ=255)
                                                                               cggtGAGGCCTATGTCAGGGTGGCCGTTGCGCCGGCGCGGTCCTATACCCAACCCCCACACCGCGTAATTCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                   <  6:20224/132‑1 (MQ=255)
                                                                               cgCTGATGCCGATGTCCAGGTGGCCGTTGAGCTGGCGCCGTTCGATCACCAACCCCGACAACTCTTAAATCGCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                   <  6:105647/134‑1 (MQ=255)
                                                                               cccTGATGCCTATGGCCTGGTGGCCGTTGCCCCGGCGCCGGTCGATCCCCACCCCCCACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                   <  2:106565/133‑1 (MQ=255)
                                                                               aGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCACCCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCg                                                  >  3:279105/1‑136 (MQ=255)
                                                                                gCTTATGCCGATGTCTAGGTTGCCGTTCACCAGCCGCCGTTCTAACAACACCCCCGACAAATCTAAAATCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTTCGGGATCAGGCTTGGCCGc                                                 <  4:345062/136‑1 (MQ=255)
                                                                                gCTGAGGCCTATGGCCCGGTTGCCGTTGAGCCGGCGCGGTGCTATCCCCACCCCCCACAACTCGAAACTCGCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGcc                                                   <  6:21410/134‑1 (MQ=255)
                                                                                  tGAGGCCGATGTCCCGGGTGCCGTTGAGCAGGCGCCGTGCCAGCCCCAACCCCCACAACTCGAAAACCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCAc                                               <  4:264102/136‑1 (MQ=255)
                                                                                   gagccggATGCCCTGGGTGCCGTTCACCAGGCGCCGTTCCACCTCAACCCCCCACAACACTTAATACCCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCAc                                               <  5:289623/129‑1 (MQ=255)
                                                                                   cctGCCGCTGCCCAGGGGGCCGGTGAGCAGGCGCCGTGCGACCTCCACCCCCGACACCTCGAAAAGCGCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGATCAAGCGGCGGCGGGATCAGGCTTGGCCGCAc                                               <  7:353304/133‑1 (MQ=255)
                                                                                     tGCCGATGACCAGGTTGCGGTTGAGCCGGCGCCGTTCGATCACCACCCCCCACAACTCGTAAAGCTCCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGt                                            <  3:158956/136‑1 (MQ=255)
                                                                                       ccGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTc                                           >  2:248198/1‑135 (MQ=255)
                                                                                              cccggTGGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCCACAACTCTTAACTCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATac                                   <  3:143198/133‑1 (MQ=255)
                                                                                                          tGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGc                          >  7:330636/1‑133 (MQ=255)
                                                                                                          tGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGc                          >  5:198583/1‑133 (MQ=255)
                                                                                                                   gcCGTTCCATCTCCACCCCCGACACCGCGTAAACCTGCACGACCAGGTGAGGGTGAGCCTTGCGCAGCCGATCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAg              <  4:129948/136‑1 (MQ=255)
                                                                                                                    ccGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGc             >  4:5503/1‑136 (MQ=255)
                                                                                                                                  aCCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGCGACTGGCCCtt  >  6:30043/1‑133 (MQ=255)
                                                                                                                                  |                                                                                                                                    
TGGGTATCGGGTATGACCAGTTGCAGCTCATCTTCGTACAACAGCAGGCCATGCAGCCCTGGCTGGCGCGGCGGCAAATAGCTGATGCCGATGTCCAGGTTGCCGTTGAGCAGGCGCCGTTCGATCTCCAACCCCGACAACTCGTAAATCTGCACGACCAGGTGAGGTTGAGCCTTGCGCAGCCGTTCAAGCAGCTGCGGGATCAGGCTTGGCCGCACGGTCTGCAATACACCGATAGCCAAGGTGCGCAGCGACTGGCCCTT  >  KT2440/444405‑444667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑