Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 482,474 A→C 33.6% V67G (GTG→GGG)  surA_1 ← Chaperone SurA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440482,4740AC33.6% 21.2 / 3.1 33V67G (GTG→GGG) surA_1Chaperone SurA
Reads supporting (aligned to +/- strand):  ref base A (15/6);  new base C (9/3);  total (24/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGGTGCCAATGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGCCACCACGCGATCAAGAGGTTGCACCGCGGCATGCACCGCGCC  >  KT2440/482344‑482607
                                                                                                                                   |                                                                                                                                     
aaTGGTGCCAATGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGCCGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCAcgcc                                                                                                                                   >  8:240463/1‑135 (MQ=255)
aaTGGTGCCAATGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCCcgcc                                                                                                                                   >  6:245543/1‑135 (MQ=255)
aaTGGTGCCAATGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCCcgcc                                                                                                                                   >  5:46332/1‑135 (MQ=255)
aaTGGTGCCAATGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCAcgcc                                                                                                                                   >  7:101203/1‑135 (MQ=255)
  tGGTGCCAATGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGAACTGCAGCTGCAGGTTCTCGACGAGCAGGCGTTCCAGGACCTGCTGTGCCAGGGCGCTGGTCCGCCGGCCcgccc                                                                                                                                  >  1:297070/1‑134 (MQ=255)
     tGCCAATGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCCcgccgccgc                                                                                                                              >  6:85222/1‑135 (MQ=255)
          aTGGCCTGGTTCAGCTCTTCGTCGTTGATGAGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGC‑TTTAAGGACCTGCTGTTCCAGGTCGCTGGTGCGGAGGAACGCCGCCGCcgcga                                                                                                                         >  7:130832/1‑134 (MQ=255)
          aTGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCCCGCCGCCGCCgcgc                                                                                                                         >  5:75969/1‑135 (MQ=255)
          aTGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCgcgc                                                                                                                         >  7:177749/1‑135 (MQ=255)
                 ggTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGTCGCTCGT‑CCGCGGCCCGCCGCCCCCGCGCTTGGCgc                                                                                                                  >  6:102179/1‑134 (MQ=255)
                  gTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCCCGCCGCCGCCGCGCTTGGCGATg                                                                                                                >  3:365549/1‑136 (MQ=255)
                           ttCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCCGCACGCCGCCGCCGCGCTTGGCGATGGTTTGCTg                                                                                                        >  7:358984/1‑135 (MQ=255)
                                            aaCCAGAGCGCGCGCCGAGCTGCCGCTGCAGGTTCGCGCCGAGCAGGCGGTCCAGCACCGGCTGGTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCt                                                                                       <  7:226536/134‑1 (MQ=255)
                                             aCCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCGTGGCGATGGTTTGATGGACCTCATGGACACGCTg                                                                                      >  8:164988/1‑135 (MQ=255)
                                                agagCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCCCGCCGCCGCCGCGCGTGGCGATGGTTTGCTGGACCTCATGGACACGCTgggc                                                                                   >  8:119973/1‑133 (MQ=255)
                                                agagCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGATGGT‑CGGGGGCACGCCCCCGCCCGGGGTGGGGGGGGTTTGCTGGGCCGCATGGGCACGCTgggc                                                                                   >  5:239515/1‑133 (MQ=255)
                                                    cgcgcgCCGAGCTGCCGCGGCGGGGTCTCCACGAGCCGGCGTTCCCGGCCCGGCTGTCCCCGGGCGCGGGG‑CGGCGGCACGCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGc                                                                               <  1:203106/135‑1 (MQ=255)
                                                     gcTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCGTGGCGATGGTTTGCTGGCCCTCATGGCCACGCTGGTCCAGCTg                                                                             >  4:137656/1‑136 (MQ=255)
                                                       tCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCCCGCCGCCGCCGCGCGTGGCGCTGGTTTGGTGGGCCTCATGGACCCCCTGGTCCCGCggg                                                                            >  8:54817/1‑135 (MQ=255)
                                                        cGCCGATCTGCCGCTGCAGGTTCTCGACGAGCAGGCGTTCAAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGAGCTTGGCGATGGTTTGCTGGTCCTCATGGTCCCGCTGGGCCAGATGGCt                                                                          >  4:272093/1‑136 (MQ=255)
                                                            gATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCGTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCttt                                                                        >  2:4971/1‑134 (MQ=255)
                                                                      tgctgGTGCGCGACGAGCCGGGGTTCACGGCCGTGGTGTGCCTGGGCGGTGGG‑CGGCGGCACGCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCAcg                                                             <  6:75969/135‑1 (MQ=255)
                                                                             tctcGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCGTGGCGATGGTTTGCTGGACCTCATGGACACGCGGGTCCCGCTGGCTTTGCCTGACCACGTCGTTGt                                                      >  8:259057/1‑135 (MQ=255)
                                                                                  acgaTCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCGTGGCGATGGTTTGCTGGACCTCATTGACACGCTGGTCCAGCTGGCGTTTCATTTCCACCACGGTGTcgac                                                  >  2:61420/1‑134 (MQ=255)
                                                                                           cGTTCCAGGACCTGCTGTGCCCGGGCGCTGGG‑CGGCGGCACGCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGccacc                                        <  2:41366/135‑1 (MQ=255)
                                                                                                cAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCTTGGCGCTGGTTTGCTGGACCTCATGGACACCCTGGTCCAGCTGGCTTTGCATGACCACCTCCTTGTCGACCCTAGCCACCAcgc                                    >  2:30222/1‑134 (MQ=255)
                                                                                                           gTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCGTGGCGATGGTTTGCTGGGCCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGCCCCCACGCGATCAAGAGGTTg                       >  4:125969/1‑136 (MQ=255)
                                                                                                           gTTCCAGGGCGCTGGT‑CCGCGGCACCCCGCCGCCGCGCTTGGCGATGGTTTGCTGGCCCTCATGGACACGCTGGTCCAGCTGGCGTTGCATGCCCACGTCGTTGTCGCCGAGAGCCCCCACGCGCTCAAGAGGtt                        >  2:6038/1‑135 (MQ=255)
                                                                                                             gcctgggCGGTGGG‑CGGGGCCCCGCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGCCACCACGCGATCAAGAGGTTGc                      <  2:70150/131‑1 (MQ=255)
                                                                                                                        gggggggcggcCCGCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGCCACCACGCGATCAAGAGGTTGCACCGCGGCAt            <  7:259057/130‑1 (MQ=255)
                                                                                                                        ggggcgggggCCCGCCGCCGCCGCGCTTGGCGATGGTGTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGCCACCACGCGATCAAGAGGTTGCACCGCGGCAt            <  7:11933/131‑1 (MQ=255)
                                                                                                                        ggggcggcgcCACCCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGCCACCACGCGATCAAGAGGTTGCACCGCGGCAt            <  7:240463/131‑1 (MQ=255)
                                                                                                                                 gcACGCCGCCGCCGCGCTTGGCGAGGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGCCACCACGCGATCAATAGGTTGCACCGCGGCATGCACCGCGcc  <  4:45141/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
AATGGTGCCAATGGCCTGGTTCAGCTCTTCGTCGGTGATGCGGATACCAGAGCGCTCGCCGATCTGCAGCTGCAGGTTCTCGACGATCAGGCGTTCCAGGACCTGCTGTTCCAGGGCGCTGGT‑CGGCGGCACGCCGCCGCCGCGCTTGGCGATGGTTTGCTGGACCTCATGGACACGCTGGTCCAGCTGGCTTTGCATGACCACGTCGTTGTCGACGATAGCCACCACGCGATCAAGAGGTTGCACCGCGGCATGCACCGCGCC  >  KT2440/482344‑482607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑