Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 655,074 C→A 9.3% D311Y (GAC→TAC)  dinF ← DNA damage‑inducible protein F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440655,0740CA 9.3% 119.6 / 4.9 43D311Y (GAC→TAC) dinFDNA damage‑inducible protein F
Reads supporting (aligned to +/- strand):  ref base C (24/15);  new base A (2/2);  total (26/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

CGCCGCCCGCACACTTTCGATGTCGGTCTGCAAGTCGATGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGCCATTGAGCAACAGGGCGTTGGCCGCCACGGTCGCTTCGCCCA  >  KT2440/654940‑655202
                                                                                                                                      |                                                                                                                                
cgccgccCGCACACTTTCGATGTCGGTCTGCAAGGCGATGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCCACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTc                                                                                                                                  <  7:213625/135‑1 (MQ=255)
cgccgccCGCACACTTTCGATGGCGGTCTGCAAGTCGATCAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTTATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTc                                                                                                                                  <  7:103081/135‑1 (MQ=255)
 gccgccCGCACACTTTCGATGTCGGTCTGCAAGTCGATGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTcg                                                                                                                                 <  2:117287/135‑1 (MQ=255)
     cccgcACACTTTCGATGTCGGTCTGCAAGTCGATGAACAGGTGCCCCCCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTc                                                                                                                            >  3:365814/1‑136 (MQ=255)
     cccgcACACTTTCGATGTCGGTCTGCAAGTCGATGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCgg                                                                                                                              >  7:35584/1‑134 (MQ=255)
       cgcACACTTTCGATGTCGGTCTGCAAGTCGATGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCAc                                                                                                                          <  4:80157/136‑1 (MQ=255)
        gcACACTTGCGATGCCGGCCTGCCAGGCGCGGACCAGGGGCCCACCCAGCAGGAACAGCCCGGCAACGCCCAGGCGGGTGATCAGGGCCCACCCCCACGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCAc                                                                                                                          <  5:40081/135‑1 (MQ=255)
            acTTTCGATGTGGGTCTGCAAGTCGATGAACAGGTGCCCACCCAGCAGGAACAGCCAGGCAAAGCCCAGGCTGGTGATCAGTGACCAAACACAAGCGACCACCACCGAGCGGCGCAAGGTGTCGCGGTCACGGGc                                                                                                                      <  8:306881/135‑1 (MQ=255)
            acTTTCGATGTCGGTCTGCAAGTCGATGAACAGGTGCCCCCCCCGCAGGAACAGCCCGGCAAAGCCCCGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGcc                                                                                                                     >  3:333994/1‑135 (MQ=255)
                      tCGGTCTGCAAGTCGATGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAatggca                                                                                                            >  2:354118/1‑135 (MQ=255)
                            tccAAGTCCATGAACAGGGCCCCACCCAGCAGGAACCGCCCGGCAAAGCCCAGGCTGGGGAGCCGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCg                                                                                                      <  5:88004/133‑1 (MQ=255)
                                 gTCGATGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACaa                                                                                                 >  8:51682/1‑135 (MQ=255)
                                     aTGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCt                                                                                            <  3:155553/136‑1 (MQ=255)
                                       gAACAGGTGCCCACCCAGCAGGAACAGCCCAGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTc                                                                                           >  5:85044/1‑135 (MQ=255)
                                          cAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCAc                                                                                        <  7:51682/135‑1 (MQ=255)
                                               gCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCAt                                                                                   <  2:27598/135‑1 (MQ=255)
                                                           ggAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCAt                                                                       <  1:354118/135‑1 (MQ=255)
                                                                aGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCCTCCAgc                                                                  >  7:295851/1‑135 (MQ=255)
                                                                  cccGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCa                                                               >  4:327292/1‑136 (MQ=255)
                                                                  cccGGCAAAGCCCAGGCTGCTGATCAGTGACCAACCACAAGCGACCTCCAGCGAGCGGCGCAAGGAGTCGCGGTCACGCGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCAAGCGCCAGCCCCTCCAgcg                                                                 >  6:321110/1‑134 (MQ=255)
                                                                    cGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCCCAAGGCCTCCCCCGCATGGGCCAGCCCATCCAgcgc                                                                >  7:236410/1‑133 (MQ=255)
                                                                    cGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTCTCGCGGTGAAGGGCACCAATTGCATGGGCGCACAACGCCTCCAACCCAATGGCCAGCCCATCCCGCGCaa                                                              >  5:229800/1‑134 (MQ=255)
                                                                     ggCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGCCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCAAACCAGCGCATAg                                                            >  4:223175/1‑136 (MQ=255)
                                                                      gCAAAGCCCAGGCTGGTGAGCAGTGCCCAACCACCAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAg                                                            <  6:233421/135‑1 (MQ=255)
                                                                       cAAAGCCCTGGCTGGTGATCAGTGACCAACCACAAGCGAGCACCAGCGAGCGTCGCAAGGTGTCTCGGTCACGGGCACCAATGGCATGGCCGCTCAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGc                                                          <  3:223175/136‑1 (MQ=255)
                                                                        aaaGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGcc                                                         <  4:89587/136‑1 (MQ=255)
                                                                         aaGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCg                                                        >  4:333297/1‑136 (MQ=255)
                                                                               aGGCTGGTGATCAGTGACCAACCACAAGCGCCCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCCGCGCATAGGCCGTca                                                     >  2:345141/1‑133 (MQ=255)
                                                                               aGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCCGCGCCTAGGCCGTcagc                                                   >  5:217529/1‑135 (MQ=255)
                                                                               aGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTcag                                                    >  3:262808/1‑134 (MQ=255)
                                                                                   tGGTGATCAGTGACCAACCACCACCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCCTAGGCCGTcagcagca                                               <  5:284936/135‑1 (MQ=255)
                                                                                         tCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGcgct                                         >  5:226434/1‑132 (MQ=255)
                                                                                         tCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGCCAt                                         >  5:317406/1‑135 (MQ=255)
                                                                                                ccaaccaCAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGCCATTGAGCa                                   <  8:35584/134‑1 (MQ=255)
                                                                                                                accaGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCCGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGCCATTGAGCAACAGGGCGTTGgccgcc                  >  8:265020/1‑135 (MQ=255)
                                                                                                                accaGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCGCCACCGCATGGGCCAGCCCCTCCAGCGCATAGGCCGTCAGCAGCAGGCCCTTGAGCAACCGGGCGTAGgccgcc                  >  2:364699/1‑135 (MQ=255)
                                                                                                                 ccaGCGAGCGGCGCAAGGTGTAGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCt                                                                                            <  5:236127/60‑1 (MQ=255)
                                                                                                                 ccaGCGAGCGGCGCAAGGTGTAGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCt                                                                                            >  6:236127/1‑60 (MQ=255)
                                                                                                                 ccaGCGAGCGGCGCAAGGTGTAGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCt                                                                                            >  6:231584/1‑60 (MQ=255)
                                                                                                                 ccaGCGAGCGGCGCAAGGTGTAGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCt                                                                                            <  5:231584/60‑1 (MQ=255)
                                                                                                                  caGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGCCATTGAGCAACAGGGCGTTGGCCGCCAc                >  8:216958/1‑135 (MQ=255)
                                                                                                                     cgagcgGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGCCATTGAGCAACAGGGCGTTGGCCGCCACGGTc            <  4:244094/136‑1 (MQ=255)
                                                                                                                          ggcgCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAgg                                             >  7:76353/1‑98 (MQ=255)
                                                                                                                          ggcgCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAgg                                             <  8:76353/98‑1 (MQ=255)
                                                                                                                               aaGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCCGCCCATCCAGCGCATAGGCCGTCAGCCGCAGGCCATTGAGCAACAGGGCGTTGGCCGCCACGGTCGCTTCGcccc  >  4:65201/1‑135 (MQ=255)
                                                                                                                               aaGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGCCATTGAGCAACAGGGCGTTGGCCGCCACGGTCGCTTCGcc    >  3:325888/1‑134 (MQ=255)
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CGCCGCCCGCACACTTTCGATGTCGGTCTGCAAGTCGATGAACAGGTGCCCACCCAGCAGGAACAGCCCGGCAAAGCCCAGGCTGGTGATCAGTGACCAACCACAAGCGACCACCAGCGAGCGGCGCAAGGTGTCGCGGTCACGGGCACCAATGGCATGGCCGCACAAGGCCTCCACCGCATGGGCCAGCCCATCCAGCGCATAGGCCGTCAGCAGCAGGCCATTGAGCAACAGGGCGTTGGCCGCCACGGTCGCTTCGCCCA  >  KT2440/654940‑655202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑