| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 96,480 | T→G | 41.2% | intergenic (+121/‑72) | cynT → / → CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 96,480 | 0 | T | G | 41.2% | 15.4 / 5.6 | 34 | intergenic (+121/‑72) | cynT/CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/16); new base G (8/6); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGTGGTG > KT2440/96353‑96608 | ctactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGgcgcgcg > 8:227510/1‑134 (MQ=255)ctactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCGGCCGTGGATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCCCCGgcccgcg > 5:132775/1‑134 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCtt < 6:156377/135‑1 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCtt < 7:363413/135‑1 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCtt < 5:293368/135‑1 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGgcgcgcgt > 2:17688/1‑133 (MQ=255) tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCGGGCATGAATTACCTGCCAGGCCAGCCGGGGGGGGCGCGGGCGGCCCCCGCCCgggcggcg > 1:335275/1‑132 (MQ=255) ccctaCCCGTCCGCCGCTAGAACCTTGATAGCCTGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAg < 1:159234/131‑1 (MQ=255) aCCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGc < 7:248170/135‑1 (MQ=255) tCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGGCAGGGGCAAACGt < 3:285355/136‑1 (MQ=255) tcgCCGGCTGCACCCTTCAGGGGTGGGGCGCGGCCTGCGGCGGCCTTGAATGCCCGGACTGCCCTGCCGGCGGGCGCGCTGGCGGCCCGCGCCTGCGCGCGTGTCCGGGGCAAACGTGCCCACGGCCAGGGGAAt < 1:215225/133‑1 (MQ=255) aGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCTGCCTTGAATTACCTGACTGCCCTGCCGGCGTGCGCGCTGGCGGCCCGCCCCGGCGCGCGTGGCAGGGGGAAAAGGGCCCCCGGCCACGGGaaat > 5:135151/1‑133 (MQ=255) ccGGCTGCACCCTTCAGGGGGGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGAGTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATgg < 7:237855/134‑1 (MQ=255) tgccggggggTGGCGCGGCATTCGGCTGGATTGATTTCCCTGACTGCCCTGCGGGGTTGCTGGCTGGCGGCCCGCGCCGGCGGTCTTGTCTGGGGCAAACTTGCCCACGGCCAGGGGAATGGCCGTGCGACTaga < 7:345063/131‑1 (MQ=255) gtgGGGGGGCCTTCGGCTGCTTTTATTTCCCTGAGTGCCTGGCGGGGGGGCGGGGGGGGGGCCGGGGCGGGGGGGTTTTTCCGGGGAATACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGgaga < 6:227284/135‑1 (MQ=255) gtgGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGgaga < 8:229638/135‑1 (MQ=255) cggggCCTTCGGGTGCCTTGAATTCCCTGACGGCCGGGCCGGCGGGCGCGCGGGCGGCCCGCGCCTGCGCGCTTTCCCGGGGCAAACGTCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 4:12194/133‑1 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAaca > 7:208962/1‑135 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGCATGGCCGTGGGGCTAGAGAAAGGAGAAacac > 3:352198/1‑136 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGGGGGGCGAGAGAAAGGAGAAaccc > 3:268322/1‑134 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGGGCGGGGGCAACGTGCCCACGGCCAGGGGGATGGCCGGGGGGCGAGAGAAAGGGGAAaca > 5:184673/1‑135 (MQ=255) cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCCCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGGCTAGAGAAAGGAGAAacaa > 3:53313/1‑135 (MQ=255) gcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:227510/135‑1 (MQ=255) gcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac < 7:342398/135‑1 (MQ=255) ggCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGt < 4:273924/136‑1 (MQ=255) ccTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGTCAGGGGCAAACGTGCCCACGGCCCGGGGTATGGCCGGGCGACTAGAGAAAGGAGCAACACgggg > 8:215139/1‑133 (MQ=255) ggCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCGGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 3:300640/136‑1 (MQ=255) gCTGCGTTGAATGCCCTGAGGGCCCGGCGGGGGGGCGCGCGGGCGGCCCGCGCCGGCGGGCTTGGCTGGGCCAAAGGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 1:171377/135‑1 (MQ=255) gCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGGTTGGCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 6:87614/135‑1 (MQ=255) gCTGCCTTGAATTCCCTGACCGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCGTGCGCGCTTGTCAGGGTCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt < 6:170139/135‑1 (MQ=255) ttCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGa < 8:208962/134‑1 (MQ=255) cTGACGGCCCGGCCGGGGGGCGCGCGGGGGGCCCGCGCCGGCGCGCTGGTCCGGGGAAACCGGGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCgg < 4:268322/136‑1 (MQ=255) ccggcgcCGGCGCGCTTGTCGGGGCCAAACGGGCCCACGGCCTGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACCCGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCggtggt < 3:304602/133‑1 (MQ=255) cgcgCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCggtggtg > 1:69182/1‑135 (MQ=255) | CTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGTCAGGGGCAAACGTGCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGTGGTG > KT2440/96353‑96608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |