Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 813,275 C→T 6.9% G235G (GGC→GGT)  ada → Bifunctional transcriptional activator/DNArepair enzyme Ada

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440813,2750CT 6.9% 176.2 / 3.5 58G235G (GGC→GGT) adaBifunctional transcriptional activator/DNArepair enzyme Ada
Reads supporting (aligned to +/- strand):  ref base C (31/23);  new base T (2/2);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGG  >  KT2440/813141‑813408
                                                                                                                                      |                                                                                                                                     
cTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggc                                                                                                                                       >  7:277999/1‑135 (MQ=255)
     gCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGgcggcg                                                                                                                                   <  8:277999/134‑1 (MQ=255)
       aaTGCTGGCTGGGGGCAATAATGGTAGCCCAGAGGGACACGGGCTTCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTGCCCCAAGGCCCACCTGATTGGCGGCGACa                                                                                                                                <  6:46445/135‑1 (MQ=255)
            tCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAgcgc                                                                                                                            >  5:171467/1‑134 (MQ=255)
            tCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGcc                                                                                                                           <  7:252066/135‑1 (MQ=255)
            tCGCTGGGTGCAATACTGGTAGACCAGAGCGCCAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCTcc                                                                                                                           >  5:9647/1‑135 (MQ=255)
                      cAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCa                                                                                                                   >  6:57259/1‑133 (MQ=255)
                       aaTACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGaa                                                                                                                <  1:199323/135‑1 (MQ=255)
                                 gCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGa                                                                                                                                  <  2:316573/107‑1 (MQ=255)
                                 gCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGa                                                                                                                                  >  1:316573/1‑107 (MQ=255)
                                 gCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGaa                                                                                                      >  6:361316/1‑135 (MQ=255)
                                 gCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGaa                                                                                                      >  1:286638/1‑135 (MQ=255)
                                 gCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAg                                                                                                     >  3:32522/1‑136 (MQ=255)
                                 gCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAg                                                                                                     >  4:170475/1‑136 (MQ=255)
                                 gCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAg                                                                                                     >  3:178014/1‑136 (MQ=255)
                                   ccAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGt                                                                                                    <  8:47496/135‑1 (MQ=255)
                                    cAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGa                                                                                                  >  3:16775/1‑136 (MQ=255)
                                      gagCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATc                                                                                                >  4:190358/1‑136 (MQ=255)
                                          gACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGt                                                                                             >  8:45041/1‑135 (MQ=255)
                                           aCAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGtt                                                                                            >  7:62921/1‑135 (MQ=255)
                                                 gCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTtcgtcg                                                                                      <  2:230260/135‑1 (MQ=255)
                                                 gCAGCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTtcgtcg                                                                                      <  2:337376/135‑1 (MQ=255)
                                                    tCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAg                                                                                   >  8:96211/1‑135 (MQ=255)
                                                    tCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAg                                                                                   <  8:265442/135‑1 (MQ=255)
                                                       gcgcGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGTGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGcc                                                                               >  3:269295/1‑136 (MQ=255)
                                                       gcgcGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgcgc                                                                                >  6:306967/1‑135 (MQ=255)
                                                       gcgcGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTG‑ACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGcc                                                                               >  7:217426/1‑135 (MQ=255)
                                                        cgcgATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgc                                                                                  <  4:32522/132‑1 (MQ=255)
                                                        cgcgATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgc                                                                                  <  4:178014/132‑1 (MQ=255)
                                                        cgcgATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgc                                                                                  <  3:343439/132‑1 (MQ=255)
                                                        cgcgATTCTGCTGGGCGACGACCCCGAGGGCGTGTTGCACGAGCTGCAGGATCAGGTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgc                                                                                  <  4:353095/132‑1 (MQ=255)
                                                         gcgATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgc                                                                                  <  5:227071/131‑1 (MQ=255)
                                                          cgATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCcct                                                                             <  5:306967/135‑1 (MQ=255)
                                                          cgATGCTGCTGGGCGACGACCCCGAGGGCGTGGTGCACGAGCTGCAGGAGCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCcct                                                                             <  2:159375/135‑1 (MQ=255)
                                                             ttCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCcctcct                                                                          >  8:17960/1‑135 (MQ=255)
                                                                tgctgGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGTGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTggg                                                                      >  3:145238/1‑136 (MQ=255)
                                                                tgctgGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGTGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTggg                                                                      >  3:139354/1‑136 (MQ=255)
                                                                   tgGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGcc                                                                    >  2:325169/1‑135 (MQ=255)
                                                                          cgacCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCcct                                                                             >  1:315489/1‑119 (MQ=255)
                                                                          cgacCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCcct                                                                             <  2:315489/119‑1 (MQ=255)
                                                                                           tgCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggcgg                                                                                                                                     >  1:335577/1‑46 (MQ=255)
                                                                                           tgCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTggcgg                                                                                                                                     <  2:335577/46‑1 (MQ=255)
                                                                                            gCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGCTGTGCAAgg                                           >  2:156758/1‑135 (MQ=255)
                                                                                             cACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGTGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCa                                         <  4:145238/136‑1 (MQ=255)
                                                                                             cACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGTGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCa                                         <  4:139354/136‑1 (MQ=255)
                                                                                             cACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGTGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCa                                         <  4:269295/136‑1 (MQ=255)
                                                                                             cACGAGCTGCAGGATCAGTTCCACAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAgg                                           <  1:340998/134‑1 (MQ=255)
                                                                                                    tGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGtg                                  >  4:279728/1‑135 (MQ=255)
                                                                                                       aGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATTGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCa                               <  3:170475/136‑1 (MQ=255)
                                                                                                          aTCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgc                                                                                  >  1:11105/1‑82 (MQ=255)
                                                                                                          aTCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAgc                                                                                  <  2:11105/82‑1 (MQ=255)
                                                                                                                tCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGGTTCAGGAGCggg                       >  1:299442/1‑135 (MQ=255)
                                                                                                                 ccccAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGgt                      >  6:42948/1‑135 (MQ=255)
                                                                                                                 ccccAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGgt                      >  8:332871/1‑135 (MQ=255)
                                                                                                                  cccAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGgtgt                    <  3:144306/136‑1 (MQ=255)
                                                                                                                      aGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCa                <  3:190358/136‑1 (MQ=255)
                                                                                                                          ccACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGgc             >  1:247711/1‑135 (MQ=255)
                                                                                                                            aCCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGTCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGgcgc           >  1:252814/1‑135 (MQ=255)
                                                                                                                            aCCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGgcgc           >  2:89266/1‑135 (MQ=255)
                                                                                                                              cTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGc        >  3:300540/1‑136 (MQ=255)
                                                                                                                               tGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGc        >  7:265673/1‑135 (MQ=255)
                                                                                                                               tGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGAGTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGc        <  2:367340/135‑1 (MQ=255)
                                                                                                                                    ggcggcGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAg   >  6:335526/1‑135 (MQ=255)
                                                                                                                                    ggcggcGACAGCGCCTACGAGCAGAAGGACGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAg   >  7:252335/1‑135 (MQ=255)
                                                                                                                                      cggcgACAGCGCCTACGAGCAGAAGGGCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCTGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAgg  <  1:162798/134‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CTTGGGCAATGCTCGCTGGGTGCAATACTGGTAGCCCAGAGCGACAAGGGCATCTGCGCGATTCTGCTGGGCGACGACCCCGAGGGCTTGTTGCACGAGCTGCAGGATCAGTTCCCCAAGGCCCACCTGATTGGCGGCGACAGCGCCTACGAGCAGAAGGTCGCCGAAGTGATCGGTTTCGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGG  >  KT2440/813141‑813408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑