Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,212,957 A→T 7.3% L17Q (CTG→CAG)  ydgC ← Inner membrane protein YdgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,212,9570AT 7.3% 164.5 / 3.3 55L17Q (CTG→CAG) ydgCInner membrane protein YdgC
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

AAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTG  >  KT2440/1212824‑1213075
                                                                                                                                     |                                                                                                                      
aaaCAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAg                                                                                                                       <  1:159945/135‑1 (MQ=255)
aaaCAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGc                                                                                                                      >  3:261858/1‑136 (MQ=255)
    aGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCa                                                                                                                     >  7:91812/1‑133 (MQ=255)
           tggtCTTGAGGGCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGcacca                                                                                                            <  7:223955/135‑1 (MQ=255)
             gtCTTGAGGTCGGCTATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACg                                                                                                          <  7:193893/135‑1 (MQ=255)
             gtCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACg                                                                                                          <  1:12163/135‑1 (MQ=255)
              tCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACg                                                                                                          >  5:53239/1‑134 (MQ=255)
                 tGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGATCAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACCGCACTGCCAGCAACAGCACCACGCCTg                                                                                                      >  8:348039/1‑135 (MQ=255)
                   aGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTgc                                                                                                     <  2:37809/134‑1 (MQ=255)
                       cGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCa                                                                                                <  2:261085/135‑1 (MQ=255)
                        ggCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCCGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCac                                                                                               >  6:357653/1‑134 (MQ=255)
                           aaTGCTGCGCCCCTTGCCGACGATCTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGCCAGCACTGCCAGCAACAGCCCCACGCCTGCGCCCAGGGc                                                                                            >  6:224100/1‑135 (MQ=255)
                                    ccccTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGaa                                                                                   <  8:305209/135‑1 (MQ=255)
                                     cccTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCACCAACAGCACCACGCCTGCGCCCAGGGCCGCGTTGAAt                                                                                  >  5:140115/1‑135 (MQ=255)
                                      ccTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATa                                                                                 <  1:42685/135‑1 (MQ=255)
                                       cTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATa                                                                                 >  8:59231/1‑134 (MQ=255)
                                          gCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACg                                                                                                          >  6:205969/1‑106 (MQ=255)
                                          gCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACg                                                                                                          <  5:205969/106‑1 (MQ=255)
                                          gCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCGTTGAATACCAgg                                                                            >  3:266376/1‑136 (MQ=255)
                                              acgaTGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTcc                                                                         <  8:91812/135‑1 (MQ=255)
                                                    tAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCGTTGAATACCAGGTGCCCgttg                                                                   >  1:280651/1‑135 (MQ=255)
                                                          gCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCa                                                            <  4:306206/136‑1 (MQ=255)
                                                           cAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCa                                                            <  2:248193/135‑1 (MQ=255)
                                                            aaTCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAg                                                           >  2:204073/1‑135 (MQ=255)
                                                            aaTCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCGTTGAATACCAGGGCCACGTTGTGTTCCAg                                                           >  8:174750/1‑135 (MQ=255)
                                                                 gggCGAAGGTGGGGAACAGCGGCAGCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTg                                                       <  5:355804/134‑1 (MQ=255)
                                                                    cGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTGCAGTTTGGGtt                                                   >  7:102455/1‑135 (MQ=255)
                                                                     gAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTtc                                                  <  1:146684/135‑1 (MQ=255)
                                                                               aaCAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCGTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCgcg                                       >  4:36957/1‑134 (MQ=255)
                                                                                aCAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAgg                                      <  4:266376/136‑1 (MQ=255)
                                                                                 cAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAgg                                      <  7:334826/135‑1 (MQ=255)
                                                                                       cACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACAACGCCTGCGCCCAGGGCCGCGTTGAATACAAGGTCCACGTTGTTTTCCCGTGTGGGGTGCGTCCGGCGGGGGCggtt                                >  5:242771/1‑132 (MQ=255)
                                                                                            gCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGGTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTAttttt                            >  2:62431/1‑134 (MQ=255)
                                                                                            gCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTACAAGTTGTTTTCCAGTTTGGGGTCAGTCAGGGGAGGCCGTAttttt                            >  8:5807/1‑134 (MQ=255)
                                                                                             cccTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTc                          <  6:47527/135‑1 (MQ=255)
                                                                                               cTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTgc                                                                                                     >  7:273174/1‑58 (MQ=255)
                                                                                               cTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTgc                                                                                                     <  8:273174/58‑1 (MQ=255)
                                                                                               cTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCAc                        >  7:264567/1‑135 (MQ=255)
                                                                                                    aTGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGGTGTTTTCCAGTTTGGGTTGAGTCAGGCGAGGCCCGATTTTTTAAAtttttg                  >  4:173165/1‑133 (MQ=255)
                                                                                                     tGTAGTAGTTGCGCGTTTTCGTCAGCTCTGCCAGCATCAGCAGGACGGCTGCGCGCAGGGGCGGTTTGAATACGAGGTCGTCGTTGTTTTTGAGTTTGGGTTCAGTCAGGCGAGGGCGTATTTTTTCACTTTGTc                  <  1:20197/135‑1 (MQ=255)
                                                                                                     tGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTGCAGTTTTGGTTGAGTCAGGCGAGGCCGTATTTTTTCAGtttt                    >  3:85516/1‑132 (MQ=255)
                                                                                                     tGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTc                  <  1:32657/135‑1 (MQ=255)
                                                                                                     tGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTc                  <  1:333984/135‑1 (MQ=255)
                                                                                                         gtagtTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACa             <  3:173165/136‑1 (MQ=255)
                                                                                                          tagtTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATa                                                                                 <  4:21624/67‑1 (MQ=255)
                                                                                                          tagtTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATa                                                                                 >  3:21624/1‑67 (MQ=255)
                                                                                                          tagtTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGGTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACa             >  2:11786/1‑135 (MQ=255)
                                                                                                             ttGCGCGTTTTCGACAGCACTGCCTGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACa             <  7:272456/132‑1 (MQ=255)
                                                                                                             ttGCGCGTTTTCGACAGCACTGCCTGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACa             >  8:267552/1‑132 (MQ=255)
                                                                                                             ttGCGCGTTTTCGACAGCACTGCCTGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACa             >  8:272456/1‑132 (MQ=255)
                                                                                                             ttGCGCGTTTTCGACAGCACTGCCTGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGGCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACa             <  7:267552/132‑1 (MQ=255)
                                                                                                               gcgcGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGACAACCAGGTGCACGGTGTTTTCCAGTTTGGGTTCAGTCCGGCGAGGCCGTATTTTTTCACTTTGTGGAACCGCGTgg       >  4:126439/1‑136 (MQ=255)
                                                                                                                  cgTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCGAGTTTGGGTTGAGTCAGGCGAGGGCGTTTTTTTTAACTTTGTCGGACCGCGTGGTc     >  5:149385/1‑135 (MQ=255)
                                                                                                                     tttCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTg  <  7:174750/135‑1 (MQ=255)
                                                                                                                     tttCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTg  <  5:370130/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                      
AAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGGGAACAGCGGCACCAGCCCTGCGATGTAGTAGTTGCGCGTTTTCGACAGCACTGCCAGCAACAGCACCACGCCTGCGCCCAGGGCCGCTTTGAATACCAGGTCCACGTTGTTTTCCAGTTTGGGTTCAGTCAGGCGAGGCCGTATTTTTTCACTTTGTCGAACAGCGTGGTCTTG  >  KT2440/1212824‑1213075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑