| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,388,322 | T→G | 36.8% | L336V (TTG→GTG) | ruvB_1 → | Holliday junction ATP‑dependent DNA helicaseRuvB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,388,322 | 0 | T | G | 36.8% | 22.6 / 2.7 | 30 | L336V (TTG→GTG) | ruvB_1 | Holliday junction ATP‑dependent DNA helicaseRuvB |
| Reads supporting (aligned to +/- strand): ref base T (15/2); new base G (7/4); total (23/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAGCGTCATACCATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCTGCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGCCACAG > KT2440/1388192‑1388455 | agagCGTCATACCATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGGCTACCTGCACTTTTGGCTGAATATTACCGGGGGCTTgggg > 3:213556/1‑136 (MQ=255) gTCATACCATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCTGCACTTTGGGCTGAATATTCCCGGGCGCGTGGGGGAggg > 4:11269/1‑136 (MQ=255) gTCATACCATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCTGCACTTTGGGCTGAATATTCCCGGGCGCATGGGGGAgg > 6:272523/1‑135 (MQ=255) aaGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCCGCATGGCTTCCTGCCCTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGAttttt > 8:59588/1‑135 (MQ=255) gATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCAACCCGCCTGCCTACCTGCACTTTTGGGGGGATATTACCGGGCCCGTGGGGGGTGGGGGCGGTTTTTcc > 6:18580/1‑135 (MQ=255) cTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCCGCATGCCTACCTGCACTTTGGGCGGGATATTCCCGGGCGCGTGGGGGAGGGGGGCGCTTTTTACGCGCCCGGCGgtgaatga > 3:88644/1‑135 (MQ=255) cTGATTCAGCAGGGCTACATCATCAGCACCCCACGAGGCCGAGTGGACACACCGCCTCCCAACCAGCACATCGGGGTGAATATTACCCGGCGCGTGGGGGCAGGTGGCGAATTTTACGACCCACGCGatggatga > 6:159158/1‑135 (MQ=255) agGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCTGCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTACGAGCCACGCGATGAATGACAGATAGAAAg > 3:271970/1‑136 (MQ=255) agGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGCCATGCCTACCTGCACGTTGGGCGGAATAATACCGGGCGCGTGGGGGCGGGGGGCGAATTTTCCGCGCCAGGCGCTGCATGACAGAGCGaaa > 6:300441/1‑135 (MQ=255) catGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCTGCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATTCATGACAGCTCGAAAGCGCCttttt > 8:195375/1‑135 (MQ=255) gcACGCCACGCGGCCGCGTGGTCCCCCGGCATGCCTACCTGCACTTTGGGCTGAATATTACCGGGCGCTTGGGGGAGGGGGGCGATTTTTACGAGCCAGGCGCTGAATGACAGGTAGAAAGCGCCgtttttttgt > 5:172499/1‑130 (MQ=255) gcACGCCACGCGGCCGCGTGGTCCCCCGGCATGCCGACCTGCACTTTGGGCTGAATATTGCCGGGCGCGTGGGGGAGGGGGGCGTTTTTTTCGCGCCCGGCGGTGAATGACACCTGGCAAGCGACGTTTTGTGGt > 2:17651/1‑135 (MQ=255) gcACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCTGCACTTTGGGCTGAATATTCCCGGGCGCGTGGGGGAGGGGGGCGATTTTTGCGCGCCAGGCGCTGAATGACCGATCGAAAGCGACTTGTTGTGGt > 8:200950/1‑135 (MQ=255) gcACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTAACGGCACTTTGGGCTGAATATTACCGGGCGCTTGGGGGCGGGGGGCGCTTTTTGCGCGCCCGGCGATGAATGACACCACGGAAACGCCGTTTTGTggg > 5:78036/1‑134 (MQ=255) gcACGCCACGCGGCCGCGGGGTCACCCGGCCTGCCTAACAGCAATTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGAGTTTTCCCCGCCAGGCCAGTAATGACAAAACGAAAGCGCCGGTTTGTggg > 5:58654/1‑134 (MQ=255) gcgcCCGGGGGGTCCCCCGGCATGCCTACCTGCCCTTGGGGCTCACTCTGCCGGGGCGCGGGGGGGCGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTg < 3:289096/136‑1 (MQ=255) gTGGTCACCCGGCATGCCTACCTGCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGGGGGGGGCGATTTTTGCCAGCCAGGCCATGAATTCCACAAAGAAACCGCCGTTTTTTGGTCCAACCGCTGGCATGcc > 6:115611/1‑135 (MQ=255) cACCCGGCATCCCCCCCGCCCCTTGGGCCGGAATTTTCCCGCGCCCGTGGGGGCGGGGGCCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGt < 4:304146/136‑1 (MQ=255) gcccccccTGCCCTGTGGGCTGAATCTGCCCGGGCGCGGGGGCGCGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGACAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCa < 4:105039/130‑1 (MQ=255) gCCGACCTGCACTTTGGGCGAAATCTGCCGGGGCGCTGGGGGCCGGGGGGCCATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCa < 8:196094/135‑1 (MQ=255) gCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGCTCGAAAGCGACGTTTTGTGGTCCTACCGCTGGCATGCCCCGGGGGCGCTGGCAATAAGACa > 1:141965/1‑135 (MQ=255) acgttGGGCTCACTAAGCCCGCGCGGGCGGGGCCGGGGGCCCCTTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACAtt < 6:311924/132‑1 (MQ=255) tatTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACATTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGaaaaaa > 3:79268/1‑135 (MQ=255) cGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGc < 3:139041/136‑1 (MQ=255) cGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGc > 4:139041/1‑136 (MQ=255) gggCGCCGGGGGGCGGGGCGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGcc < 3:11269/136‑1 (MQ=255) gcTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTTATGAAGAAAAAAAAGTTGCCACAg > 4:36412/1‑136 (MQ=255) gcTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGCCACAg > 4:210148/1‑136 (MQ=255) gcTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTACTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGCcaca > 8:350955/1‑135 (MQ=255) gcTTGGGGGAGGGGGGCGAATTTTCCGACCCAGGCGATGAAAGACACATCGAAAGCGACTTTTTGTTGTGCTACCGCGGGCATGCCCCAGGTGCGCAGGCAATAACTCAATAATGAAGAAAAAACAGTTGcccaa > 2:40738/1‑132 (MQ=255) | AGAGCGTCATACCATCGAAGATGTGCTTGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGCACGCCACGCGGCCGCGTGGTCACCCGGCATGCCTACCTGCACTTTGGGCTGAATATTCCCGGGCGCTTGGGGGAGGGGGGCGATTTTTCCGAGCCAGGCGATGAATGACAGATCGAAAGCGACTTTTTGTGGTCCTACCGCTGGCATGCCCCTGGTGCGCTGGCAATAAGACATTAATGAAGAAAAAACAGTTGCCACAG > KT2440/1388192‑1388455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |