Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,525,921 C→T 6.1% T292T (ACC→ACT)  secA → Protein translocase subunit SecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,525,9210CT 6.1% 201.1 / 3.2 65T292T (ACC→ACT) secAProtein translocase subunit SecA
Reads supporting (aligned to +/- strand):  ref base C (33/28);  new base T (2/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GATCATTTCCGGTCAGGCCGAGGACAGCTCCAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGCTGA  >  KT2440/1525798‑1526051
                                                                                                                           |                                                                                                                                  
gATCATTTCCGGTCAGGCCGAGGACAGCTCCAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGagaa                                                                                                                         >  7:243305/1‑135 (MQ=255)
  tCATTTCCGGTCAGGCCGAGGACAGCTCCAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGagaaga                                                                                                                       >  6:134484/1‑135 (MQ=255)
          ggTCAGGCCGAGGACAGCTCCAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAg                                                                                                               <  5:158248/135‑1 (MQ=255)
            tCAGGCCGAGGACAGCTCCAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTg                                                                                                            <  4:116583/136‑1 (MQ=255)
              aGGCCGAGGACAGCTCCAAGCTGTACCTCGAGATCAACCGCCTGATCTCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGt                                                                                                             >  5:257061/1‑133 (MQ=255)
              aGGCCGAGGACAGCTCCAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGt                                                                                                             >  1:233887/1‑133 (MQ=255)
                   gAGGACAGCTCCAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCt                                                                                                       >  6:193450/1‑134 (MQ=255)
                            gcaattcTGTAATTCTAGAACAACCGCTGGAGCCCGCGCCGCACCCCGCACAGCAAAGGGGGCGATGGCCTGGTCACCCAGTAAGGCCACTTCACCATGGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAg                                                                                               <  2:364497/127‑1 (MQ=255)
                             ccAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAg                                                                                               >  4:154424/1‑132 (MQ=255)
                             ccAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAg                                                                                               <  3:154424/132‑1 (MQ=255)
                              cAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCgg                                                                                           >  1:229266/1‑135 (MQ=255)
                              cAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGt                                                                                          >  3:338074/1‑136 (MQ=255)
                                      agaTCGATGTCAACCGCCTGATCCCGGGCCTCACCCAGCACTTCGAAGAGGTCGAAGGACAGGTCACCCATGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGt                                                                                   <  3:38830/133‑1 (MQ=255)
                                       cagctagaTCACCCGCCTGATCCCGCGCCTCACCCAGCACATCTAAGCGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGtt                                                                                  <  7:358947/130‑1 (MQ=255)
                                        aTCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTc                                                                                 >  8:36047/1‑135 (MQ=255)
                                        aTCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTc                                                                                 >  1:13433/1‑135 (MQ=255)
                                         tCGAGATCAACCGCCTGATCCCGCGCCTCACCCCGCACATCGAAGAGGTCGCAGGCCAGGTCACCCAGGAAGGCCCCTTCACCATCGACGAGAAGAGTCGCCAGGGGGAGCTGACCGAAGCCGCTCACCAGttac                                                                                >  7:325242/1‑133 (MQ=255)
                                            agaTCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGACGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCg                                                                             <  2:329984/135‑1 (MQ=255)
                                             gaTCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCg                                                                             >  3:365689/1‑134 (MQ=255)
                                               tCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGa                                                                            >  6:339785/1‑133 (MQ=255)
                                                 acccGCCTGAGCCCGCGCCTCCCCCAGCACAGCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAagag                                                                        <  8:257373/133‑1 (MQ=255)
                                                  aCCGCGTCATCTCGCGCCTCACCCAGCACATCGACGAGGTCGAAGGCCAGGTCACCCAGGAAGGCAACTTCACCATCGACTAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAagag                                                                        <  6:257061/134‑1 (MQ=255)
                                                     gCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGc                                                                    >  5:81289/1‑135 (MQ=255)
                                                         gATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGcc                                                               >  3:16791/1‑136 (MQ=255)
                                                         gATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGc                                                                >  6:19363/1‑135 (MQ=255)
                                                                 gcCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAagag                                                                                                                      <  6:136896/73‑1 (MQ=255)
                                                                 gcCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAagag                                                                                                                      >  5:136896/1‑73 (MQ=255)
                                                                   cTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGc                                                      >  1:229620/1‑135 (MQ=255)
                                                                               aTCGAAGAGGTCGACGGCCAGGGCACCCAGGAAGCCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAg                                         <  4:16791/136‑1 (MQ=255)
                                                                               aTCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATTCTGGCCCAGGCGGGCCTGCTGGCTGaa                                          >  6:257400/1‑135 (MQ=255)
                                                                               aTCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGaa                                          >  2:89745/1‑135 (MQ=255)
                                                                               aTCGAAGAGGTCGAAGGCCAGGGCCCCCAGGAAGGCCACTTCACCATCGACGAGAAGAGGCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAg                                         <  4:26990/136‑1 (MQ=255)
                                                                                tCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAg                                         <  8:201206/135‑1 (MQ=255)
                                                                                tCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAg                                         <  5:339785/135‑1 (MQ=255)
                                                                                tCGAAGAGGGCGAAGGCCAGGTCACCCAGGAAGTCCACTTCACCATCGACGAGAAGAGTCGCCAGGCGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAg                                         <  8:18108/135‑1 (MQ=255)
                                                                                tCGAAGAGCTCGACGGCCAGGTCACCCCGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAg                                         <  6:115920/135‑1 (MQ=255)
                                                                                 cgacgaGGGCGAACGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGGCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGc                                       <  3:285840/136‑1 (MQ=255)
                                                                                 cGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGGGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGc                                       <  3:353946/136‑1 (MQ=255)
                                                                                  taatagGTCGAAGGCCAGGTCACCCAGGACGGCCACTTCACCATCGACGATAAGAGTCGCGAGGGGGAGCTGAACGAAGCCTGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTTAAGGc                                       <  2:69606/131‑1 (MQ=255)
                                                                                  gacgagGGCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGc                                       <  1:87670/132‑1 (MQ=255)
                                                                                  gAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGc                                       >  6:181661/1‑135 (MQ=255)
                                                                                   aaGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAgg                                        >  8:281768/1‑133 (MQ=255)
                                                                                   aaGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGc                                       <  2:233887/134‑1 (MQ=255)
                                                                                    agagGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAgg                                        >  8:10340/1‑132 (MQ=255)
                                                                                    agagGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGc                                       >  3:170002/1‑133 (MQ=255)
                                                                                           gAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCt                               >  8:318344/1‑134 (MQ=255)
                                                                                            aaGGCCAGGTCACCCAGGCAGGCCACTTCACCATCGACGAGACCAGTCGCCAGGAGGAGCTGACCGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGt                             <  6:81289/135‑1 (MQ=255)
                                                                                            aaGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGt                             >  2:96252/1‑135 (MQ=255)
                                                                                            aaGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGt                             >  5:339038/1‑135 (MQ=255)
                                                                                            aaGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTa                            >  4:319032/1‑136 (MQ=255)
                                                                                                    gTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCcgcg                     >  2:85395/1‑135 (MQ=255)
                                                                                                            ggAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTg            >  4:361034/1‑136 (MQ=255)
                                                                                                                 gCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCt        <  7:10340/135‑1 (MQ=255)
                                                                                                                     cTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGCTg   <  4:170002/136‑1 (MQ=255)
                                                                                                                      ttCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACCACCTGCGCTTGCTGa  >  3:207426/1‑136 (MQ=255)
                                                                                                                      ttCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGCTg   <  1:85395/135‑1 (MQ=255)
                                                                                                                      ttCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGCTg   <  7:281768/135‑1 (MQ=255)
                                                                                                                      ttCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGCTg   <  5:193450/135‑1 (MQ=255)
                                                                                                                      ttCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCAGGGCTTGCTGa  >  3:280271/1‑136 (MQ=255)
                                                                                                                        cACTATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCCTCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGc     <  8:352547/131‑1 (MQ=255)
                                                                                                                        cACTATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGc     >  7:352547/1‑131 (MQ=255)
                                                                                                                        cACTATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGc     <  8:352550/131‑1 (MQ=255)
                                                                                                                        cACTATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTCGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCAGCGCACAACCTGGGCTTGc     >  7:352550/1‑131 (MQ=255)
                                                                                                                        cACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGCAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGc                                       >  5:323784/1‑97 (MQ=255)
                                                                                                                        cACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGc                                       <  6:323784/97‑1 (MQ=255)
                                                                                                                           |                                                                                                                                  
GATCATTTCCGGTCAGGCCGAGGACAGCTCCAAGCTGTACATCGAGATCAACCGCCTGATCCCGCGCCTCACCCAGCACATCGAAGAGGTCGAAGGCCAGGTCACCCAGGAAGGCCACTTCACCATCGACGAGAAGAGTCGCCAGGTGGAGCTGAACGAAGCCGGTCACCAGTTCATCGAAGAGATGCTGGCCCAGGCGGGCCTGCTGGCTGAAGGCGAGAGCCTGTACTCCGCGCACAACCTGGGCTTGCTGA  >  KT2440/1525798‑1526051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑