Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,593,255	A→G	6.7%	S328P (TCC→CCC)	CJ019_01499 ←	Beta‑xylosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,593,255	0	A	G	6.7%	169.2 / 3.8	61	S328P (TCC→CCC)	CJ019_01499	Beta‑xylosidase
Reads supporting (aligned to +/- strand):  ref base A (30/24);  new base G (2/2);  total (35/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

TTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGAATCAGCTCCTGGATGG  >  KT2440/1593134‑1593386                                                                                                                          |                                                                                                                                    ttCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGcc                                                                                                                         <  1:364770/134‑1 (MQ=255) ttCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGcc                                                                                                                         <  1:74789/134‑1 (MQ=255)   cAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCCCCGGCCTTCAGCAGGCCCgg                                                                                                                      >  5:322143/1‑135 (MQ=255)      aTCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCCCCTCGCCGGACTTCAGCAGGCCCGGCAGc                                                                                                                  >  4:213853/1‑136 (MQ=255)          gTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGctct                                                                                                               <  1:128057/135‑1 (MQ=255)              cGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGcc                                                                                                          >  3:65997/1‑136 (MQ=255)               ggCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGcc                                                                                                          <  4:217408/135‑1 (MQ=255)                gCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGcc                                                                                                          <  7:108482/134‑1 (MQ=255)                gCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCg                                                                                                         <  7:31272/135‑1 (MQ=255)                 ccTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGt                                                                                                        <  2:202984/135‑1 (MQ=255)                   ttGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCCCGCACCGCCTGGTCCAGCTCGCGCGGGGGCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCgga                                                                                                       >  3:217614/1‑132 (MQ=255)                   ttGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACGTCAGCAGGCCCGGCCGCTCTGCGCCGt                                                                                                        >  7:150474/1‑133 (MQ=255)                     gCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAg                                                                                                    <  4:65997/135‑1 (MQ=255)                       cGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAggg                                                                                                  <  2:215679/135‑1 (MQ=255)                        gATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGCCTTCAGCAGGCCCGGCAGCTCTTCGCCGGCCAGGGt                                                                                                 >  5:267516/1‑135 (MQ=255)                        gATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGctct                                                                                                               >  5:135578/1‑121 (MQ=255)                        gATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGctct                                                                                                               <  6:135578/121‑1 (MQ=255)                          tGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATc                                                                                              <  3:359547/136‑1 (MQ=255)                           gcgcACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATc                                                                                              <  6:210671/135‑1 (MQ=255)                                          cTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTc                                                                               >  4:313555/1‑135 (MQ=255)                                              aaCAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGc                                                                          >  3:316907/1‑136 (MQ=255)                                                      cATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGGCTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCt                                                                    >  8:159128/1‑134 (MQ=255)                                                      cATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGGCTTCAGCAGGCCCGGCAGCTCGTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCt                                                                    >  8:154748/1‑134 (MQ=255)                                                      cATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCa                                                                                                                    >  1:231852/1‑86 (MQ=255)                                                      cATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCa                                                                                                                    <  2:231852/86‑1 (MQ=255)                                                      cATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCt                                                                    >  8:19312/1‑134 (MQ=255)                                                      cATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGGACAGGGTATCGTTCATGCTCATGTCGATTCCGGCCtt                                                                   >  3:367860/1‑135 (MQ=255)                                                      cATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCAGGGTCACCTCGCCGGACTTCAGCAGGCCCGGAAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCtt                                                                   >  4:170819/1‑135 (MQ=255)                                                      cATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTAGCGCTGGGTCACCTCGCCGGACTTCACCAGGCCCCGCCCCGCATCGCCGTACACGGTATCGGAGATGCACATGTCGATGCCGGccg                                                                    >  5:202243/1‑133 (MQ=255)                                                         gTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGAt                                                                <  5:311151/135‑1 (MQ=255)                                                                ttGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGCCGTCAGCAGGCCCGGCAGCTCTTCGCCGGACAGGGGAGCGTTCCTGCTCATGTCGATGCCGGCCTTGATCGCCAGc                                                         >  5:299866/1‑135 (MQ=255)                                                                ttGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGcc                                                                                                          >  2:83060/1‑86 (MQ=255)                                                                ttGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGcc                                                                                                          <  1:83060/86‑1 (MQ=255)                                                                ttGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTGATGTCGATGCCGGCCTTGATCGCCAGCt                                                        >  4:81675/1‑136 (MQ=255)                                                                ttGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGc                                                         >  7:73547/1‑135 (MQ=255)                                                                ttGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATAGCCAGc                                                         >  7:195884/1‑135 (MQ=255)                                                                ttGGAACCCAGCACTTCACGCACCGCCTGGTCCAGCTAGCGAGGGGTCACATCGACTGACTTCACCATGCCCCGCACCTCTTCGCCGTAAAGGGTATCGTTCATGCTCAATTAGATGACCGCCTTGATAGCCAGc                                                         >  8:319465/1‑135 (MQ=255)                                                                    cACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTgg                                                     <  8:73547/135‑1 (MQ=255)                                                                         cgCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGACCGGCAGCTCTTAGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCt                                                <  6:205892/134‑1 (MQ=255)                                                                            aCTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCa                                             <  5:82283/135‑1 (MQ=255)                                                                            aCTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCa                                             >  2:64074/1‑135 (MQ=255)                                                                            aCTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTAATGTCGATGCCGGCCTTGATCGCCCGCTTTGCGGCTTCa                                             >  2:156464/1‑135 (MQ=255)                                                                                 aCGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGcc                                        >  2:294435/1‑135 (MQ=255)                                                                                      ccGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCAc                                   >  6:100853/1‑135 (MQ=255)                                                                                         ccTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACggg                                >  7:352758/1‑135 (MQ=255)                                                                                            ggTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGAc                            >  4:251212/1‑136 (MQ=255)                                                                                                 aGCTCGCGCTGGGTCACCTCTCCGGACTTCAGCAGGCCCGGCTGCTCTTCGCCGTACAGTGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCAAGATTGGCGGATTCACGGCAGTCACGGGCGACGCCg                        <  7:319465/135‑1 (MQ=255)                                                                                                      gcgcTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGcc                                                                                                          >  8:101408/1‑48 (MQ=255)                                                                                                      gcgcTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGcc                                                                                                          <  7:101408/48‑1 (MQ=255)                                                                                                      gcgcTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGc                    >  8:82161/1‑134 (MQ=255)                                                                                                       cgcTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGa                  <  7:109926/135‑1 (MQ=255)                                                                                                       cgcTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGa                  <  1:294435/135‑1 (MQ=255)                                                                                                          tGGGTCACCTCGCCGGGCTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGAATc               <  7:159128/135‑1 (MQ=255)                                                                                                          tGGGTCACCTCGCCGGGCTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGAATc               <  7:154748/135‑1 (MQ=255)                                                                                                            ggTCACCTCGCCTGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGGTTCACGGCCGTCACGGGCGACGCCGTGGCGAATCAg             <  6:202243/135‑1 (MQ=255)                                                                                                             gTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGAATCAGCt           <  4:316907/136‑1 (MQ=255)                                                                                                               cACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGCCGCCGGGGCGAATCAGGTc          >  6:16370/1‑135 (MQ=255)                                                                                                                aCCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGAATCAGCTcc         >  6:126290/1‑135 (MQ=255)                                                                                                                     gCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGAATCAGCTCCTGGAt    >  7:273083/1‑135 (MQ=255)                                                                                                                     gCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGAATCAGCTCCTGGATg   >  4:140815/1‑136 (MQ=255)                                                                                                                       cGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGCCGCCGTGGCGAATCAGCTCCTGGATgg  >  2:117036/1‑135 (MQ=255)                                                                                                                          |                                                                                                                                    TTCAGATCGGTTTCGGCCTTGCCGATGCGCACGTACGGGTCCTTGAACAGGCCCATGTCGTACTTGGCACCCAGCACTTCACGCACCGCCTGGTCCAGCTCGCGCTGGGTCACCTCGCCGGACTTCAGCAGGCCCGGCAGCTCTTCGCCGTACAGGGTATCGTTCATGCTCATGTCGATGCCGGCCTTGATCGCCAGCTTGGCGGCTTCACGGCCGTCACGGGCGACGCCGTGGCGAATCAGCTCCTGGATGG  >  KT2440/1593134‑1593386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑