Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,628,470 A→G 8.0% E221E (GAA→GAG)  era → GTPase Era

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,628,4700AG 8.0% 149.0 / 2.6 49E221E (GAA→GAG) eraGTPase Era
Reads supporting (aligned to +/- strand):  ref base A (17/28);  new base G (2/2);  total (19/30)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGATCATTTCTTCCCCGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTCCGAGGCGCGCAAGGACA  >  KT2440/1628337‑1628600
                                                                                                                                     |                                                                                                                                  
aCGATCATTTCTTCCCCGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGaa                                                                                                                                    >  1:217948/1‑134 (MQ=255)
aCGATCATTTCTTCCCCGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAAt                                                                                                                                   <  4:291444/135‑1 (MQ=255)
     cATTTCTTCCCCGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAag                                                                                                                              <  6:234478/135‑1 (MQ=255)
       tttCTTCCCCGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAagcag                                                                                                                           >  4:249321/1‑136 (MQ=255)
       tttCTTCCCCGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTACGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAagcag                                                                                                                           >  4:174932/1‑136 (MQ=255)
             ccccGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAgg                                                                                                                       <  3:315250/134‑1 (MQ=255)
             ccccGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGCGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGTAATTCAAGCAGCAgg                                                                                                                       <  3:251228/134‑1 (MQ=255)
              cccGACGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAgg                                                                                                                       <  7:328531/133‑1 (MQ=255)
              cccGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGcc                                                                                                                    <  4:68904/136‑1 (MQ=255)
              cccGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGGTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAgg                                                                                                                       <  6:232998/133‑1 (MQ=255)
               ccGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCa                                                                                                                   >  4:222049/1‑136 (MQ=255)
                cGAAGACCAGATCACCGTCCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCAt                                                                                                                  <  4:289282/136‑1 (MQ=255)
                cGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCa                                                                                                                   >  5:302647/1‑135 (MQ=255)
                cGAAGACAAGATCACCGACCGTAGCAGCCGCTTCATGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTAACGCAGCAGGGCCa                                                                                                                   >  7:46035/1‑135 (MQ=255)
                 gAAGACCAGATCACCGCCCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCAt                                                                                                                  <  5:7716/135‑1 (MQ=255)
                            cACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACAt                                                                                                       <  8:5667/135‑1 (MQ=255)
                             accgaccgTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACAt                                                                                                       >  1:241623/1‑134 (MQ=255)
                                  ccgTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCAcgcg                                                                                                >  3:343234/1‑136 (MQ=255)
                                        cagcCGCTTCCTGGCCGCCGAACTGGTGCGCGAGCAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGCTCGAGGAGTTCCAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGAt                                                                                           >  5:242749/1‑135 (MQ=255)
                                        cagcCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAGTTCAAGCAGCAGGGCCCTGTGCTGCACATTCACGCGTTGAt                                                                                           >  5:247474/1‑135 (MQ=255)
                                                tCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTg                                                                                   >  7:172385/1‑135 (MQ=255)
                                                   tGGCCGCCGAACTGGTGCGCGAGAAGATAATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACCGTGGAGATCGAGGAATTCAACCAGCAGCGCCATGTGCTGCACAATCACGCGGTGATACTGGTTGACcg                                                                               >  4:220201/1‑136 (MQ=255)
                                                       cgccgAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAATCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGGTGAACGCGa                                                                            >  2:328958/1‑135 (MQ=255)
                                                                   gcgcGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAgaagaa                                                                <  8:116138/135‑1 (MQ=255)
                                                                    cgcgAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGaaa                                                               >  6:59473/1‑135 (MQ=255)
                                                                         gaagaTCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAatca                                                           >  8:17666/1‑134 (MQ=255)
                                                                          aagaTCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGTAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAatcatc                                                         <  8:89352/135‑1 (MQ=255)
                                                                          aagaTCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAatcatc                                                         <  2:33209/135‑1 (MQ=255)
                                                                          aagaTCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAatcatc                                                         <  2:66444/135‑1 (MQ=255)
                                                                           agaTCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAatcatc                                                         <  6:302647/134‑1 (MQ=255)
                                                                                aTGCGCCAGCGGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTg                                                  <  4:19468/136‑1 (MQ=255)
                                                                                 tGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTg                                                  <  2:241623/135‑1 (MQ=255)
                                                                                    gcCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGa                                                 >  8:216503/1‑133 (MQ=255)
                                                                                         cTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGc                                          <  1:145917/135‑1 (MQ=255)
                                                                                                      tGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAgcg                            >  4:98596/1‑136 (MQ=255)
                                                                                                      tGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAgc                             >  7:111184/1‑135 (MQ=255)
                                                                                                       gCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAgcg                            <  3:249321/135‑1 (MQ=255)
                                                                                                       gCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAgcg                            <  3:220201/135‑1 (MQ=255)
                                                                                                        ccGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAgcg                            <  6:330853/134‑1 (MQ=255)
                                                                                                        ccGTACCAGATCACGGTGGAGAGCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAgcg                            <  5:264925/134‑1 (MQ=255)
                                                                                                          gTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATCTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTCACAAGGGCGAGCGCATCAAGCGCAt                         >  8:99639/1‑135 (MQ=255)
                                                                                                                gagcACGGTGGAGCTCGAGGAATTCACGCAGCACGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTGGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTcc                  <  4:164279/133‑1 (MQ=255)
                                                                                                                 atcctggTGGAGAGCGAGGAGTTCAAGCCGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTcc                  <  6:242749/130‑1 (MQ=255)
                                                                                                                 aTCACGGTGGAGATCGAGGAGTTCAAGCAGGAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTcc                  <  6:247474/135‑1 (MQ=255)
                                                                                                                       gTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCCTCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTCCGAGgcg            <  2:217948/135‑1 (MQ=255)
                                                                                                                        tGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGTCCATAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTCCGAGgcgcg          <  4:9205/136‑1 (MQ=255)
                                                                                                                              tCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTCCGAGGCGCGCAAgg     <  2:229110/135‑1 (MQ=255)
                                                                                                                                 aGGAATTCACGCAGCATGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGACATAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTCCGAGGCGCTCAAGGACa  <  2:319483/135‑1 (MQ=255)
                                                                                                                                   gAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTCCGAGgcgcg          <  7:339769/125‑1 (MQ=255)
                                                                                                                                   gAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTCCGAGgcgcg          >  8:339769/1‑125 (MQ=255)
                                                                                                                                     |                                                                                                                                  
ACGATCATTTCTTCCCCGAAGACCAGATCACCGACCGTAGCAGCCGCTTCCTGGCCGCCGAACTGGTGCGCGAGAAGATCATGCGCCAGCTGGGTGCGGAGTTGCCGTACCAGATCACGGTGGAGATCGAGGAATTCAAGCAGCAGGGCCATGTGCTGCACATTCACGCGTTGATCCTGGTTGAACGCGATGGCCAGAAGAAAATCATCATTGGTGACAAGGGCGAGCGCATCAAGCGCATCGGTTCCGAGGCGCGCAAGGACA  >  KT2440/1628337‑1628600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑