Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,699,833 T→C 7.9% F281S (TTC→TCC)  CJ019_01595 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,699,8330TC 7.9% 145.5 / 4.4 51F281S (TTC→TCC) CJ019_01595hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/24);  new base C (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

CTGGTGCCGATGGACAACGGCATCCGCCTGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCGGCGTCGGCAGCA  >  KT2440/1699706‑1699963
                                                                                                                               |                                                                                                                                  
cTGGTGCCGATGGACAACGGCATCCGCCTGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGCCCCTACCTGGCGCCAGGTGATGCTGCGGTTCCGCgat                                                                                                                             >  5:28321/1‑135 (MQ=255)
cTGGTGCCGATGGACAACGGCATCCGCCTGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCgat                                                                                                                             >  1:139139/1‑135 (MQ=255)
      ccGATGGACAACGGCATCCGCCTTTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATccc                                                                                                                       <  8:184926/135‑1 (MQ=255)
             aCAACGGCATCCGCCTGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCAtt                                                                                                                <  1:126229/135‑1 (MQ=255)
             aCAACGGCAGCCGCCTGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCAtt                                                                                                                <  2:259928/135‑1 (MQ=255)
                    cATCCGCCTGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCAGACACAACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCAATTCAAAgg                                                                                                         >  1:331760/1‑135 (MQ=255)
                      tCCGCCGGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCt                                                                                                      <  4:251250/136‑1 (MQ=255)
                         gCCTGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTgc                                                                                                    >  2:4418/1‑135 (MQ=255)
                           cggTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTCCCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTgcggc                                                                                                 <  4:85402/134‑1 (MQ=255)
                            tGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTgcggc                                                                                                 <  2:144713/135‑1 (MQ=255)
                                   gCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTg                                                                                           <  2:9764/134‑1 (MQ=255)
                                    cACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGcc                                                                                         <  7:80149/135‑1 (MQ=255)
                                    cACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGcc                                                                                         <  5:223191/135‑1 (MQ=255)
                                         gcCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGt                                                                                    <  2:5470/135‑1 (MQ=255)
                                           caccacCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGCCCTTCACCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGCTCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGc                                                                                  >  1:126135/1‑135 (MQ=255)
                                           caccacCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGcc                                                                                 >  3:79315/1‑136 (MQ=255)
                                           caccacCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGc                                                                                  >  5:268301/1‑135 (MQ=255)
                                                      gTGCTGAAACTGACCCGAGGGGGGCCCGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCAATTTCACCGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTg                                                                      >  4:165147/1‑136 (MQ=255)
                                                         ctgcAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCTACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACg                                                                   <  4:358072/136‑1 (MQ=255)
                                                             aaCTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACgag                                                                 <  5:164561/134‑1 (MQ=255)
                                                             aaCTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACgag                                                                 >  3:221909/1‑134 (MQ=255)
                                                             aaCTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACga                                                                  >  1:115528/1‑133 (MQ=255)
                                                              aCTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGagaa                                                               <  2:284196/135‑1 (MQ=255)
                                                                  aCCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACa                                                          <  3:57141/136‑1 (MQ=255)
                                                                    ccGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACaa                                                         <  8:97953/135‑1 (MQ=255)
                                                                     cGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAg                                                        >  4:35337/1‑135 (MQ=255)
                                                                     cGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAg                                                        >  4:40228/1‑135 (MQ=255)
                                                                      gcggggggCACGTGCCGACCTTCGCCGACCCTACCTCGCGCCAGGTGATGCTGCGGTGCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGc                                                       <  6:69692/133‑1 (MQ=255)
                                                                          ggggCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCaa                                                     >  6:292670/1‑133 (MQ=255)
                                                                           gggCACGTGCAGACCTTCGCCGACCCTACCTGGCGCGAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGa                                                  <  5:320582/135‑1 (MQ=255)
                                                                            ggCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGt                                                <  3:10187/136‑1 (MQ=255)
                                                                             gCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCAAATTTCCCTGACGAGAAGAACAAGCAATGAGt                                                <  6:28321/135‑1 (MQ=255)
                                                                               aCGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGt                                                >  2:31/1‑133 (MQ=255)
                                                                                 gTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTgaa                                             >  5:339656/1‑134 (MQ=255)
                                                                                 gTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTCCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTgaag                                            >  3:109667/1‑135 (MQ=255)
                                                                                 gTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTCCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTgaag                                            >  3:104277/1‑135 (MQ=255)
                                                                                     aGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTGCTCGATGATGCCCGCCATTTCAACGGCCTGCGGCGGCGTGGCGCAGTGCCCCAATTGCCGGACGTGAAGAACAAGCAAAGAGTGAAGaacg                                        >  5:208086/1‑135 (MQ=255)
                                                                                          ttCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGaacgcaacg                                   <  2:70647/135‑1 (MQ=255)
                                                                                          ttCGCCGACCCTACCTGGCGCCAGGTGATGCGGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGaacgcaacg                                   <  2:258130/135‑1 (MQ=255)
                                                                                            cGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGcc                                 >  6:60698/1‑135 (MQ=255)
                                                                                                    cTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATc                              <  7:157773/130‑1 (MQ=255)
                                                                                                    cTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATc                              >  8:157773/1‑130 (MQ=255)
                                                                                                      aCCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACgag                                                                 >  1:211030/1‑93 (MQ=255)
                                                                                                      aCCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACgag                                                                 <  2:211030/93‑1 (MQ=255)
                                                                                                              ccAGGTGATGCTGCGGTCCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCg              <  4:109667/136‑1 (MQ=255)
                                                                                                              ccAGGTGATGCTGCGGTCCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCg              <  4:104277/136‑1 (MQ=255)
                                                                                                                 ggTGATGCTGCGGTTACTCTATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCGGc            <  1:86399/135‑1 (MQ=255)
                                                                                                                   tGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCGGCGt          <  5:60698/135‑1 (MQ=255)
                                                                                                                        ctgcGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCGGCGTCGgca     >  7:222219/1‑135 (MQ=255)
                                                                                                                        ctgcGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCGGCGTCGgca     >  2:194577/1‑135 (MQ=255)
                                                                                                                           cGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCGGCGTCGgcagca  >  2:332522/1‑135 (MQ=255)
                                                                                                                               |                                                                                                                                  
CTGGTGCCGATGGACAACGGCATCCGCCTGTATCAGCACGCGCCACCACCGCGGGTGCTGCAACTGACCCGAGGGGGGCACGTGCAGACCTTCGCCGACCCTACCTGGCGCCAGGTGATGCTGCGGTTCCTCGATGATCCCAGCCATTTCAACGGCCTGCGGCGGCTTGCCGAAGTGCCCAATTTCCCTGACGAGAAGAACAAGCAATGAGTGAAGAACGCAACGCCATCCCGCTGATCCTGACCGGCGTCGGCAGCA  >  KT2440/1699706‑1699963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑