Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,805,331 C→T 100% R48H (CGC→CAC)  dmlR_4 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,805,3310CT100.0% 208.8 / ‑6.8 60R48H (CGC→CAC) dmlR_4HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (36/22);  minor base G (0/2);  total (36/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATC  >  KT2440/1805203‑1805463
                                                                                                                                |                                                                                                                                    
gTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTg                                                                                                                               >  4:90459/1‑136 (MQ=255)
   cGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCa                                                                                                                             <  5:166408/135‑1 (MQ=255)
      tGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGc                                                                                                                          >  1:28282/1‑135 (MQ=255)
      tGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGc                                                                                                                          >  7:114868/1‑135 (MQ=255)
      tGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCg                                                                                                                         >  4:196580/1‑136 (MQ=255)
         ccagggcgcccagggcgcGCAGCGCTTCCTCACGGCCATCCTGCATGCGTTGGCTATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCg                                                                                                                         <  7:325169/133‑1 (MQ=255)
                gCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGc                                                                                                               >  4:272077/1‑136 (MQ=255)
                   cATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCgg                                                                                                            <  3:196580/136‑1 (MQ=255)
                    aTGGCGCGCAGCGCTGCCTCACGGCCATCCTGCAAGCGGTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCgg                                                                                                            <  1:355361/135‑1 (MQ=255)
                     tGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCggg                                                                                                           >  8:47816/1‑135 (MQ=255)
                       gcgcgcAGCGCTTCCTCACGGCCATCCTGCAAGCGGTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGa                                                                                                        <  4:3354/136‑1 (MQ=255)
                                  ttCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCCGGCGGTCCTCCAGCCGGGCGATCTGCCGGCt                                                                                              >  8:265185/1‑135 (MQ=255)
                                     cTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGAt                                                                                           >  6:158916/1‑135 (MQ=255)
                                       cACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCa                                                                                                                             <  3:154974/99‑1 (MQ=255)
                                       cACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCa                                                                                                                             >  4:154974/1‑99 (MQ=255)
                                         cGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCTGCCGGGCGATCTGCCGGCTGATGTGc                                                                                       <  6:45207/135‑1 (MQ=255)
                                            ccATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATg                                                                                   <  4:46500/136‑1 (MQ=255)
                                             gaTCCTGGAAGCGTTGGCTATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCTGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGTGCGATCTGCCGGCTGATGTGCGATg                                                                                   <  5:360583/134‑1 (MQ=255)
                                             cttCCTGCAAGCGTTGGCAATGGTGCAGGAAGGTCTGCACGGCTTCGCTCAGGGTTACACGGGTGGTGCTTCGATACTGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGATGATGTGCGATg                                                                                   <  2:332529/133‑1 (MQ=255)
                                             cATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATg                                                                                   <  8:234211/135‑1 (MQ=255)
                                             cATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGa                                                                                  <  4:329519/136‑1 (MQ=255)
                                                  tGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGGGGTCCTCCAGCCGGGCGATCTGCCGGCGGCTGTGCGATGAGGaa                                                                              >  5:280916/1‑135 (MQ=255)
                                                  tGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGaaa                                                                             >  4:242289/1‑136 (MQ=255)
                                                         gTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCa                                                                       >  1:314010/1‑135 (MQ=255)
                                                         gTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAg                                                                      >  3:82281/1‑136 (MQ=255)
                                                             gCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCCGGCGGTCCTCCAGCCGGGCGATGTGCCGGCTGATGTGCGATGAGGACAACCCCAGGCGt                                                                  >  4:80006/1‑136 (MQ=255)
                                                             gCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGACACCCCCCGGCGt                                                                  >  4:46473/1‑136 (MQ=255)
                                                               aaTGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGCTCTGCCGGCTGATGTGCGATGAGGAACCCCCCCCGCGTTc                                                                >  4:1567/1‑136 (MQ=255)
                                                               aaTGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAcccc                                                                         >  1:212778/1‑127 (MQ=255)
                                                               aaTGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAcccc                                                                         <  2:212778/127‑1 (MQ=255)
                                                               aaTGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGtt                                                                 >  5:373440/1‑135 (MQ=255)
                                                               aaTGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTc                                                                >  3:339384/1‑136 (MQ=255)
                                                                 tgctgcAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTcgg                                                              <  3:16334/136‑1 (MQ=255)
                                                                  gctgcAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTcgg                                                              <  5:200288/135‑1 (MQ=255)
                                                                         gAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATAAAGCAGGTGAGTTTGCAGGCGGTCCTCCAGCCGGGCGCTCTGCCGGCTGAGTTGCGATGATGAAACCCCCAGTCCTTcggcggcgg                                                        >  3:36596/1‑134 (MQ=255)
                                                                              tCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGa                                                  >  6:372372/1‑135 (MQ=255)
                                                                                 gCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACt                                               >  5:95336/1‑135 (MQ=255)
                                                                                          cGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCCGCCGGGCGATCTGCCGGCGGCTGTGCGATGAGGAAACCCCCAGGCGGTCGGCGGCGGCAGTGCACTGGCCCGCTTc                                     >  3:210862/1‑136 (MQ=255)
                                                                                          cGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGCGCGATCTGGCGGCTGATGGGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGccccca                                        >  6:209120/1‑131 (MQ=255)
                                                                                                    tACCCGGCGGGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGc                            >  2:63200/1‑135 (MQ=255)
                                                                                                       ccGGCGGGTGCTGCGATACAGCAGGGGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCTGCAGTGAACTGGCCCGATTCGGCCACGGCGACa                        <  3:310026/136‑1 (MQ=255)
                                                                                                        cggcggGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACa                        <  1:1738/135‑1 (MQ=255)
                                                                                                         ggcggGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGCTCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGAATCGGCCACGGCGACaa                       >  2:305562/1‑135 (MQ=255)
                                                                                                         ggcggGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTcgg                                                              >  8:69780/1‑96 (MQ=255)
                                                                                                         ggcggGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTcgg                                                              <  7:69780/96‑1 (MQ=255)
                                                                                                         ggcggGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGCACTGGCCCGCTTCGGCCACGGCGACaa                       >  8:9759/1‑135 (MQ=255)
                                                                                                         ggcggGTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCCCGGCGACa                        >  5:325389/1‑134 (MQ=255)
                                                                                                              gTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGCTCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATtcg                  >  2:128121/1‑135 (MQ=255)
                                                                                                              gTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGCCAAATtcg                  >  7:262775/1‑135 (MQ=255)
                                                                                                              gTGCTGCGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATtcgt                 >  4:304122/1‑136 (MQ=255)
                                                                                                              gTGCTGCGATACAGCAGGTGCGGTTTCAGGCGGTCCTACAGCCGGGCGCTATGCCGCCAGATGTGAGATGAGGAAACCCCCACGCGTTCGGCGCGGGCAGTGAACTGGCCCGATTCGGCCCCGGCCACAAATtcgt                 >  4:279845/1‑136 (MQ=255)
                                                                                                                gctgcGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATtcgtc                >  7:360366/1‑135 (MQ=255)
                                                                                                                    cGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGCAAAACCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCCCGGCGACAAATTAGTAGaag            >  1:136247/1‑133 (MQ=255)
                                                                                                                    cGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGCACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGCTg            >  7:103761/1‑135 (MQ=255)
                                                                                                                    cGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGAt             <  4:147574/134‑1 (MQ=255)
                                                                                                                    cGATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGAt             <  4:359432/134‑1 (MQ=255)
                                                                                                                     gATACAGCAGGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGAt             <  7:309834/133‑1 (MQ=255)
                                                                                                                            cagGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCAt   <  3:242289/136‑1 (MQ=255)
                                                                                                                             agGTGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCAt   <  5:372372/135‑1 (MQ=255)
                                                                                                                             agGGGCGTTTGCAGGCGGGCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATc  <  3:1567/136‑1 (MQ=255)
                                                                                                                                |                                                                                                                                    
GTTCGCTGGCCAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATC  >  KT2440/1805203‑1805463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑