| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,138,813 | C→T | 6.3% | A37V (GCT→GTT) | CJ019_02027 → | Putative low molecular weight protein‑tyrosine‑phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,138,813 | 0 | C | T | 6.3% | 203.8 / 3.4 | 63 | A37V (GCT→GTT) | CJ019_02027 | Putative low molecular weight protein‑tyrosine‑phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (34/25); new base T (2/2); total (36/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
ACGGCGCTTGCTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATCGCACTTCGCCGAATACG > KT2440/2138683‑2138947 | aCGGCGCTTGCTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGCCAAGGTGCAGGTGGCTTCTg > 4:361070/1‑136 (MQ=255)aCGGCGCTTGCTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCt > 8:129380/1‑135 (MQ=255)aCGGCGCTTGCTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCt > 7:291279/1‑135 (MQ=255)aCGGCGCTTGCTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCt > 2:109787/1‑135 (MQ=255)aCGGCGCTTGCTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCt > 7:132265/1‑135 (MQ=255) ggCGCTTGCTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGc > 2:86096/1‑135 (MQ=255) cTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCAGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTCCGCCATCTATTGCACGCCGCCGGGCTCGCTGACAAGGTGCCGGTGGCTTCTGCCGCCAcccg > 3:50728/1‑134 (MQ=255) cgcgTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTc > 2:241236/1‑135 (MQ=255) cgcgTCCTGGTCGTGGGCCGGCGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCg < 3:224397/136‑1 (MQ=255) cgTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCAACCGCCGAAGGCTTGCTGCGCCATCAATTGCACGCCGCCGTGCTCGCTGACAAAGTGCAGGTGGCTTCTACCGGCACCGGCGATTGACATGTCg > 1:68978/1‑134 (MQ=255) tGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAgg > 2:72665/1‑135 (MQ=255) tGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAgg > 6:231106/1‑135 (MQ=255) tCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCTCCGGCGATTGGCATGTCGGCTAGgcgc > 1:252304/1‑135 (MQ=255) gtgtGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGgcgc > 4:39004/1‑133 (MQ=255) gtgtGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCa > 6:362375/1‑135 (MQ=255) gtgtGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCa > 8:180984/1‑135 (MQ=255) gtgCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGAc < 3:325569/136‑1 (MQ=255) gCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACa > 2:360030/1‑135 (MQ=255) gCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCCATTGGCATGTCGGCAAGGCGCCAGACa > 1:289335/1‑135 (MQ=255) ccggggCAACAGCTGCCGCGCGCCCCCCGCCGACGGGGGGCTGCGCCATCAATTCCCCGCCGCCGGGCTCGCTGACACGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAg < 6:57925/132‑1 (MQ=255) ccTGGGCAACATCTGCCGCGCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAg < 1:61144/135‑1 (MQ=255) gggCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCACCAGACAGCCg > 8:31400/1‑135 (MQ=255) gCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCAAGTCGGCAAGGCGCCAGACAGCCGCa > 5:57925/1‑135 (MQ=255) aTCTGCCGCGCGCCCACCGCCGAAGGCGTGCTGCGCCAGCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCa < 4:242659/136‑1 (MQ=255) tCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCa < 1:18930/135‑1 (MQ=255) cTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAg > 3:240494/1‑136 (MQ=255) cTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAggcggc > 5:165392/1‑135 (MQ=255) cccACCGCCGACGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTg < 7:287713/135‑1 (MQ=255) cccACCGCCGACGGCGTGCGGCGCCATCAATGGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTg < 7:24346/135‑1 (MQ=255) ccACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGTTTCTGCCGGCACCGGCGATTGGCATGTCGGCACGGCGCCAGACCGCCGCCCCTGCAAGGCGGCGCTgg > 5:8159/1‑135 (MQ=255) ccACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGTTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTgg > 5:3321/1‑135 (MQ=255) ccACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTgg > 1:277947/1‑135 (MQ=255) cACGGCGGACGGCGTGCTGCGCAATCAATTCCCCGCGGCGGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGgc < 5:167590/135‑1 (MQ=255) cACCGCCGACGGCGTGCTGCGCCATCAATTGCCCGCCGCCGGGCGCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGgc < 8:163529/135‑1 (MQ=255) ctgcGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGt > 3:110622/1‑136 (MQ=255) gcgcCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTc > 6:145285/1‑135 (MQ=255) aaTTGCCCGCCGCCGGGCGCGCTGACAAGGGGCCGGTGGCTTCTGCCGGCACCGGCGATTGGCATGGCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAAc < 5:40357/135‑1 (MQ=255) aTTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAAcgc > 4:271012/1‑136 (MQ=255) cccgccgcCGGGCTCGCGGACCAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGcc < 5:272119/133‑1 (MQ=255) aCGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGcc < 7:94490/134‑1 (MQ=255) aCGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGcc < 2:277947/134‑1 (MQ=255) aCGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGcc < 7:129380/134‑1 (MQ=255) gccgccGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCa > 2:29676/1‑134 (MQ=255) gccgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACCGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCACCGCGCCCAACAgg > 4:10244/1‑136 (MQ=255) gccgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAgg > 4:351299/1‑136 (MQ=255) gccgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAgg < 4:166687/136‑1 (MQ=255) ccgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGGCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAgg < 2:24110/135‑1 (MQ=255) cgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGTCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGcc > 3:222173/1‑127 (MQ=255) cgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGgc < 3:147789/96‑1 (MQ=255) cgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGgc > 4:147789/1‑96 (MQ=255) cgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCTCGCGGTTACGACCTGTCGCAGCAACGCGcc < 4:222173/127‑1 (MQ=255) cgGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGCTTACGACCTGTCGCAGCAACGCGCCCAACAgg > 8:223978/1‑134 (MQ=255) gggCTCGCGGAGACGGGGCAGGTGGCTGCTGCCGGCACCGGCGATTGGCATGGCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTc < 5:112048/135‑1 (MQ=255) ggCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGACATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGt > 1:263253/1‑133 (MQ=255) tCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAg > 8:17374/1‑134 (MQ=255) tGACAAGGTGCAGGTGGTTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATc < 6:3321/135‑1 (MQ=255) tGACAAGGGGCCGGGGGTTTCTGCCGGCACCGGCGATTGGCATGGCGGCACGGCGCCAGACAGCCGCACCTGCACGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATc < 6:8159/135‑1 (MQ=255) aGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGcc > 3:51821/1‑107 (MQ=255) aGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGcc < 4:51821/107‑1 (MQ=255) aGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATCGCACTTCGCCg < 1:77282/135‑1 (MQ=255) aGGTGGCTTCTGCCGGCACCGGCGATTGGCATGGCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATCGCACTTCGCCg < 2:15241/135‑1 (MQ=255) ggCTTCTGCCGGCACCGGCGATTGGCATGGCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCGGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATCGCACTTCGCCGAATAc < 3:361070/136‑1 (MQ=255) gCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATCGCACTTCGCCGAATAc < 3:351299/135‑1 (MQ=255) cTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATCGCACTTCGCCGAATACg < 5:145285/135‑1 (MQ=255) | ACGGCGCTTGCTGGAGGCCTGATGCGCGTCCTGTTCGTGTGCCTGGGCAACATCTGCCGCTCGCCCACCGCCGAAGGCGTGCTGCGCCATCAATTGCACGCCGCCGGGCTCGCTGACAAGGTGCAGGTGGCTTCTGCCGGCACCGGCGATTGGCATGTCGGCAAGGCGCCAGACAGCCGCACCTGCAAGGCGGCGCTGGCGCGCGGTTACGACCTGTCGCAGCAACGCGCCCAACAGGTCAAGGCATCGCACTTCGCCGAATACG > KT2440/2138683‑2138947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |