Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,371,171 G→A 8.2% R251H (CGC→CAC)  zntB → Zinc transport protein ZntB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,371,1710GA 8.2% 149.9 / 4.4 49R251H (CGC→CAC) zntBZinc transport protein ZntB
Reads supporting (aligned to +/- strand):  ref base G (18/27);  new base A (2/2);  total (20/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCCCGCAACGGGAAATCTACGCGCAGTTGTCGCGCAGCAAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGGCTGCTGGGCATCAATGTCGG  >  KT2440/2371036‑2371306
                                                                                                                                       |                                                                                                                                       
ccccGCAACGGGAAATCTACGCGCAGTTGTCGCGCAGCAAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAAcg                                                                                                                                         <  3:174412/136‑1 (MQ=255)
     cAACGGGAAATCTACGCGCAGTTGTCGCGCAGCAAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACgcgctg                                                                                                                                    <  4:363635/136‑1 (MQ=255)
     cAACGGGAAATCTACGCGCAGTTGTCGCGCAGCAAATGGAGCTGGGTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACgcgctg                                                                                                                                    <  4:94773/136‑1 (MQ=255)
      taCGGGAAATCTGCGGGCAGTTGTCGGGCTGCAAATGGTGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACgcgctg                                                                                                                                    <  5:226156/134‑1 (MQ=255)
      aaagtgAAATCTACCAGCAGTTGTCGCGCAGAAATTGGAGCTGGTTCGCCAATGCCCACGCAGATTACTGGAATCAACTCAACAACAGCCTGATCCTCTACCTCGAAGAGCTGGAACTGGCCCGCGAACgcgctg                                                                                                                                    <  8:271958/130‑1 (MQ=255)
      aaCGGGAAATCTACGCGCAGTTGTCGCGCAGCAAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACgcgctg                                                                                                                                    <  5:121318/135‑1 (MQ=255)
                             tCGCGCAGCAAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGAc                                                                                                             >  1:9816/1‑135 (MQ=255)
                              cgcgCAGCAAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACcg                                                                                                           <  3:352898/136‑1 (MQ=255)
                               gcgcAGCAAATGCAGCTGGCTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGAGCTGGAAAGCGAGGACcg                                                                                                           <  1:208149/135‑1 (MQ=255)
                                cgcAGCAAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACcgg                                                                                                          <  5:270135/135‑1 (MQ=255)
                                    gcaAAGGCAGCGGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGACCGCGCTGCGCTGGTGCTGGAAAGCGAGGACCggcggc                                                                                                      <  7:162059/135‑1 (MQ=255)
                                     caAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACATCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGgcggcg                                                                                                     >  6:28265/1‑135 (MQ=255)
                                     caAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCggcggc                                                                                                      >  6:146672/1‑134 (MQ=255)
                                     caAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCggcggc                                                                                                      >  8:100076/1‑134 (MQ=255)
                                      aaaTGGAGCAGGTGCGCCAAAGCCGACGCCGATTACTGGAAAGAACTCAACAACAGCCAGATCCGCTACCTCGACGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCa                                                                                                   <  3:45763/136‑1 (MQ=255)
                                            aGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGACCTCCACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCagcgag                                                                                              <  1:77153/135‑1 (MQ=255)
                                                ggTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGAt                                                                                         >  4:291097/1‑136 (MQ=255)
                                                   tCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGa                                                                                       <  7:283103/135‑1 (MQ=255)
                                                    cGCCAATGCCGACGCAGATTACTGGACTGACCTCAACAACCGCCTGATCCGGTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAAc                                                                                     <  4:486/136‑1 (MQ=255)
                                                     gCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAAcc                                                                                    <  4:262636/136‑1 (MQ=255)
                                                      ccAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAAcc                                                                                    <  6:93187/135‑1 (MQ=255)
                                                      ccAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAAcc                                                                                    <  6:60887/135‑1 (MQ=255)
                                                      ccAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAAcc                                                                                    <  1:305159/135‑1 (MQ=255)
                                                       cAATCCCGACGCCGATTACTGGAATCACCTCAACAACAGCCTGAGCCGCTACCTCGAAGAGCGGGAACTGGCCCGCGAACGCGCTGCGCTGGGGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCg                                                                                   <  1:277585/135‑1 (MQ=255)
                                                         aTGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCACGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCa                                                                                 >  5:144201/1‑135 (MQ=255)
                                                             cGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCAt                                                                             <  8:6784/135‑1 (MQ=255)
                                                              gACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCAt                                                                             >  7:344547/1‑134 (MQ=255)
                                                                  ccGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCg                                                                       >  3:202427/1‑136 (MQ=255)
                                                                     aTTACTGGAATGAACTCAACAACATCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCt                                                                     <  5:28265/135‑1 (MQ=255)
                                                                        aCTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTgg                                                                 >  3:33600/1‑136 (MQ=255)
                                                                        aCTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGCAACTGGCCCGCGAACGCGCTCCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGGTTTg                                                                  >  2:245122/1‑135 (MQ=255)
                                                                               tGAACTCAACAACAGCCTGATCCGCTACCGCGAAGCGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGcatcat                                                           <  5:210463/135‑1 (MQ=255)
                                                                                  aCTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCAcc                                                       <  3:157592/136‑1 (MQ=255)
                                                                                   cTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCt                                                      <  3:291097/136‑1 (MQ=255)
                                                                                    tCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCAcca                                                      >  1:104546/1‑134 (MQ=255)
                                                                                    tCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCt                                                      >  1:47841/1‑135 (MQ=255)
                                                                                    tCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACACGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCt                                                      >  7:298850/1‑135 (MQ=255)
                                                                                    tCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACACGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCt                                                      >  7:293390/1‑135 (MQ=255)
                                                                                                   tCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGca                                                               <  8:201170/111‑1 (MQ=255)
                                                                                                   tCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGca                                                               >  7:201170/1‑111 (MQ=255)
                                                                                                          ccTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCttt                               >  3:355352/1‑136 (MQ=255)
                                                                                                          ccTCGAAGAGCTGGAACTGGACCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCTCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCttt                               >  4:338571/1‑136 (MQ=255)
                                                                                                           cTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTg                              <  3:83837/136‑1 (MQ=255)
                                                                                                            tCGAAGAGCTGGACCTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTg                              <  1:270286/135‑1 (MQ=255)
                                                                                                            tCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTg                              <  1:28261/135‑1 (MQ=255)
                                                                                                            tCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTg                              <  8:344547/135‑1 (MQ=255)
                                                                                                                       gAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCa                           <  2:239428/127‑1 (MQ=255)
                                                                                                                       gAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCa                           >  1:239428/1‑127 (MQ=255)
                                                                                                                        aaCTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGgctg                   <  2:47841/134‑1 (MQ=255)
                                                                                                                        aaCTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGgctg                   <  8:268729/134‑1 (MQ=255)
                                                                                                                        aaCTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGgct                    <  5:106775/133‑1 (MQ=255)
                                                                                                                        aaCTGGCCCGCGAACACGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGgctg                   <  8:293390/134‑1 (MQ=255)
                                                                                                                        aaCTGGCCCGCGAACACGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGgctg                   <  8:298850/134‑1 (MQ=255)
                                                                                                                               ccGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCAAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGGCTGCTGGGCAt           >  4:305771/1‑135 (MQ=255)
                                                                                                                                     aCGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCCTCATCACCTGCCTCTTCCTGCCCATGAGCTGTGTCACCGGGCTGCTGGGCATCAgtgt     >  6:248268/1‑135 (MQ=255)
                                                                                                                                       gcgctgcgctGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGGCTGCTGGGCATCAATGTcgg  <  4:202427/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CCCCGCAACGGGAAATCTACGCGCAGTTGTCGCGCAGCAAATGGAGCTGGTTCGCCAATGCCGACGCCGATTACTGGAATGAACTCAACAACAGCCTGATCCGCTACCTCGAAGAGCTGGAACTGGCCCGCGAACGCGCTGCGCTGGTGCTGGAAAGCGAGGACCGGCGGCGCAGCGAGCGGATGAACCGCACCATGTACCGCTTTGGCATCATCACCTGCATCTTCCTGCCCATGAGCTTTGTCACCGGGCTGCTGGGCATCAATGTCGG  >  KT2440/2371036‑2371306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑