Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,488,613 C→G 6.6% R101P (CGC→CCC)  CJ019_02346 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,488,6130CG 6.6% 161.1 / 2.0 56R101P (CGC→CCC) CJ019_02346hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/17);  new base G (2/2);  total (37/19)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGATTCTTCTATCAGCGATTGCA  >  KT2440/2488479‑2488745
                                                                                                                                      |                                                                                                                                    
ggcgCAGGCCTTATCTGCTCCGGCCCAGTAGCAGGCCTACCGCCATCCCGAGGCCGGCAGAAATAGCATCAGTCTGCCAGGTGGTGCAGCCGATGTATGCCTGGGTGGGGTACACCACCGGTTTCGACTttctgc                                                                                                                                      <  5:123980/135‑1 (MQ=255)
ggcgCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACCGTCTGCCAGGGGTGGCCGCCGAGGTAGTCCTGGGTGGCGTCCCCCCCCCGTAGCGCCTtgctgc                                                                                                                                      >  2:331814/1‑135 (MQ=255)
ggcgCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTtgctgc                                                                                                                                      >  7:87726/1‑135 (MQ=255)
ggcgCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTtgctgc                                                                                                                                      <  5:123975/135‑1 (MQ=255)
    cAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGGGCAc                                                                                                                                  >  6:303118/1‑135 (MQ=255)
    cAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGGGCAc                                                                                                                                  >  6:298168/1‑135 (MQ=255)
                    cGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCATAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCCCCGGTTGCGCCTTGCTGCGCCCGTTGCTGGCCGCCTgg                                                                                                                  >  6:47679/1‑135 (MQ=255)
                    cGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTgg                                                                                                                  >  7:212671/1‑135 (MQ=255)
                    cGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTgg                                                                                                                  >  8:132536/1‑135 (MQ=255)
                    cGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTgg                                                                                                                  >  5:294991/1‑135 (MQ=255)
                    cGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGc                                                                                                                 >  4:360579/1‑136 (MQ=255)
                    cGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGc                                                                                                                 >  4:212608/1‑136 (MQ=255)
                      gCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCCCGTTGCTGGCCGCCTGGCg                                                                                                                >  5:230403/1‑135 (MQ=255)
                      gCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTgg                                                                                                                  >  8:312451/1‑133 (MQ=255)
                           gTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTg                                                                                                          <  3:212608/136‑1 (MQ=255)
                            tAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTg                                                                                                          <  5:155469/135‑1 (MQ=255)
                            tAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTg                                                                                                          <  1:263583/135‑1 (MQ=255)
                                 ggCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGaa                                                                                                     <  8:87726/135‑1 (MQ=255)
                                  gCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGGGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAg                                                                                                    <  5:303118/135‑1 (MQ=255)
                                  gCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGGGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAg                                                                                                    <  5:298168/135‑1 (MQ=255)
                                    cTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGCCGAAGtt                                                                                                  >  1:142301/1‑135 (MQ=255)
                                      aCCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGtt                                                                                                  >  6:209793/1‑133 (MQ=255)
                                         gCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCCCCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGCGCCTGCCGCAGGTTCAgg                                                                                             >  7:330321/1‑135 (MQ=255)
                                         gCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCCGGTGCGCCTTGCTTCGGACGTTGCTGGCCGCCTGGCGCGCCGGACGAAGTTTCAgg                                                                                             >  5:22628/1‑135 (MQ=255)
                                               ccGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTAcc                                                                                       >  1:86783/1‑135 (MQ=255)
                                               ccGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCt                                                                                      >  4:310566/1‑136 (MQ=255)
                                                cGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCt                                                                                      >  8:14676/1‑135 (MQ=255)
                                                    gCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGgc                                                                                  <  7:79381/135‑1 (MQ=255)
                                                    gCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGGCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGgcg                                                                                 <  4:169429/136‑1 (MQ=255)
                                                        gCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGgcgcgc                                                                              <  5:357988/135‑1 (MQ=255)
                                                               tAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGtct                                                                       <  5:209793/135‑1 (MQ=255)
                                                                 gCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTcgcg                                                                     >  8:204719/1‑135 (MQ=255)
                                                                    aCAGGCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGct                                                                  <  1:345680/135‑1 (MQ=255)
                                                                      aGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGcttct                                                               <  4:117189/136‑1 (MQ=255)
                                                                         cTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGAAGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCttcttc                                                             >  2:105284/1‑135 (MQ=255)
                                                                           gCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGcc                                                          >  4:149641/1‑136 (MQ=255)
                                                                             cAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGcc                                                          <  2:247169/134‑1 (MQ=255)
                                                                             cAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGCCTTCTTCGCcca                                                        >  4:167863/1‑136 (MQ=255)
                                                                               ggggTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCcca                                                        >  8:107795/1‑134 (MQ=255)
                                                                                   tGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAgg                                                   >  5:111345/1‑135 (MQ=255)
                                                                                     gccgccGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCCCCAGggc                                                 >  6:105267/1‑135 (MQ=255)
                                                                                                aGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCATGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCg                                       <  5:57611/134‑1 (MQ=255)
                                                                                                    cTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCAGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACATGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTca                                   >  8:347639/1‑134 (MQ=255)
                                                                                                          ggCGTCCACCACCGGTTGCGCCTTGCTGCGCCCGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCCCGGTCTCGGCTTCTTAGCCCCCCACGGCGGCACGGTCGGTGAGCCGttt                            >  6:250468/1‑135 (MQ=255)
                                                                                                          ggCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTg                                                                                                          <  6:4896/57‑1 (MQ=255)
                                                                                                          ggCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTg                                                                                                          >  5:4896/1‑57 (MQ=255)
                                                                                                          ggCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTg                                                                                                          <  6:346868/57‑1 (MQ=255)
                                                                                                          ggCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTg                                                                                                          >  5:346868/1‑57 (MQ=255)
                                                                                                          ggCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCCCCAGGGCGGCACTGTCGTTCAGCAGttt                            >  2:311845/1‑135 (MQ=255)
                                                                                                          ggCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGtttt                           >  3:7621/1‑136 (MQ=255)
                                                                                                          ggCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGttt                            >  7:265617/1‑135 (MQ=255)
                                                                                                              tCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCGCGGCTTCTTCGCCCCCCAGGGCGGCACGGTCGGTCAGCAGtttttgc                        >  5:69420/1‑133 (MQ=255)
                                                                                                               ccaccaCCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCg                       >  7:68411/1‑135 (MQ=255)
                                                                                                               ccaccaCCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCACGGCGGCACTGTCGGTCAGCAGTTTTTACg                       >  6:1429/1‑135 (MQ=255)
                                                                                                                       gggtGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGATtcttct               <  6:286254/132‑1 (MQ=255)
                                                                                                                       ggTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGATtcttct               <  6:294991/135‑1 (MQ=255)
                                                                                                                           gcgcCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGa                      <  7:278370/124‑1 (MQ=255)
                                                                                                                           gcgcCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGa                      >  8:278370/1‑124 (MQ=255)
                                                                                                                           gcgcCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTAGCCCACCAGGGCGGCACAGTCGGTCAGCACTTTTTTCGATTCTTCTATAAg          >  4:5158/1‑136 (MQ=255)
                                                                                                                           gcgcCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCGCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGATTCTTCTATCa           >  5:71608/1‑135 (MQ=255)
                                                                                                                                    tgcGGACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGATTCTTCTATCAGCGATTGCa  <  6:111345/135‑1 (MQ=255)
                                                                                                                                    tgcGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGATTCTTCTATCAGCGATTGCa  >  7:26561/1‑135 (MQ=255)
                                                                                                                                     gcgcACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCgg                                                                    >  2:177108/1‑68 (MQ=255)
                                                                                                                                     gcgcACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCgg                                                                    <  1:177108/68‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GGCGCAGGCCTTAACTGCGCCGGCCCAGTAGCAGGCCTACCGCCAGCCCGAGGCCGGCAGAAATAGCAACAGTCTGCCAGGGGTGGCCGCCGATGTAGTCCTGGGTGGCGTCCACCACCGGTTGCGCCTTGCTGCGCACGTTGCTGGCCGCCTGGCGAGCCTGACGAAGTTTCAGGCTTACCTGGGCGCGCAGGGTCTCGGCTTCTTCGCCCACCAGGGCGGCACTGTCGTTCAGCAGTTTTTCCGATTCTTCTATCAGCGATTGCA  >  KT2440/2488479‑2488745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: