Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,529,790 A→T 5.9% V769E (GTG→GAG)  mrpA ← Na( )/H( ) antiporter subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,529,7900AT 5.9% 209.1 / 3.8 68V769E (GTG→GAG) mrpANa( )/H( ) antiporter subunit A
Reads supporting (aligned to +/- strand):  ref base A (28/35);  new base T (2/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

GGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATGTCGTTGGGCGTC  >  KT2440/2529657‑2529924
                                                                                                                                     |                                                                                                                                      
ggTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCcac                                                                                                                                       <  5:222454/135‑1 (MQ=255)
ggTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCcac                                                                                                                                       <  7:201960/135‑1 (MQ=255)
ggTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCcac                                                                                                                                       <  7:44617/135‑1 (MQ=255)
 gTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCcaca                                                                                                                                      <  2:158721/135‑1 (MQ=255)
     cAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGccc                                                                                                                                  >  6:365459/1‑134 (MQ=255)
     cAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGcca                                                                                                                                  >  7:145266/1‑135 (MQ=255)
          gCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCCCCTGGCGTTGTTGCGCCGGCAACTGCATGCTAGCTTTCGGAGGGCGGACGCGCCGTAGCAGGGCGAACACGGTCAATGCCCCAGCCACCAATa                                                                                                                            >  3:123568/1‑136 (MQ=255)
          gCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAAt                                                                                                                             >  8:267365/1‑135 (MQ=255)
           cATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGGCAATGCCACAGCCACCAATa                                                                                                                            <  8:82058/135‑1 (MQ=255)
             tGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCCCCAATACg                                                                                                                          >  2:147615/1‑135 (MQ=255)
               ccGCGGGTTGATCAGGTCGGTGACCACGTCTGTCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGt                                                                                                                        >  7:68015/1‑135 (MQ=255)
               ccGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATTCGGt                                                                                                                        <  6:258830/135‑1 (MQ=255)
               ccGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACgg                                                                                                                         >  7:191003/1‑134 (MQ=255)
               ccGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGt                                                                                                                        <  5:284992/135‑1 (MQ=255)
                  cgGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGAt                                                                                                                     >  2:33473/1‑135 (MQ=255)
                   gggTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGAttt                                                                                                                   >  3:323897/1‑136 (MQ=255)
                     gTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCCACTGCATTCGCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACCGCCCCCACTAAGGTGATGTc                                                                                                                  >  5:38274/1‑135 (MQ=255)
                     gTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTc                                                                                                                  >  5:67193/1‑135 (MQ=255)
                     gTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTc                                                                                                                  <  6:273284/135‑1 (MQ=255)
                     gTTGATCAGGGCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTc                                                                                                                  <  2:190482/135‑1 (MQ=255)
                            aGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCTCAGCCACCAATACGGTGATTTCACCGAgg                                                                                                           >  1:145520/1‑135 (MQ=255)
                                           tCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCcgcg                                                                                             >  2:6168/1‑134 (MQ=255)
                                           tCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCcgcg                                                                                             >  1:286147/1‑134 (MQ=255)
                                               gcgcCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGAGTATCGAAGCCGCGGAAGTc                                                                                       <  4:350760/136‑1 (MQ=255)
                                                cgcCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCTCAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTc                                                                                       <  2:145520/135‑1 (MQ=255)
                                                cgcCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTc                                                                                       <  2:286147/135‑1 (MQ=255)
                                                cgcCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTc                                                                                       <  8:295731/135‑1 (MQ=255)
                                                cgcCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTc                                                                                       <  1:255887/135‑1 (MQ=255)
                                                cgcCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTATTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTc                                                                                       <  1:118710/135‑1 (MQ=255)
                                                     aCTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCa                                                                                  <  7:283864/135‑1 (MQ=255)
                                                     aCTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCa                                                                                  <  8:145266/135‑1 (MQ=255)
                                                       tGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGCACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAg                                                                                 >  1:361525/1‑134 (MQ=255)
                                                       tGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGc                                                                                >  1:149488/1‑135 (MQ=255)
                                                           gttgttGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGTTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGAc                                                                           <  4:322001/136‑1 (MQ=255)
                                                               ttgCGCGGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACAt                                                                         >  8:113523/1‑134 (MQ=255)
                                                               ttgCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACAtt                                                                        <  3:133375/135‑1 (MQ=255)
                                                               ttgCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACAtt                                                                        <  3:97372/135‑1 (MQ=255)
                                                               ttgCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACAt                                                                         <  1:33473/134‑1 (MQ=255)
                                                                tgCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACAtt                                                                        <  1:302308/134‑1 (MQ=255)
                                                                   gcCGGCAACTGCATGCGCTCTTTCGGAGGGCGGAATCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGAcc                                                                    <  1:301570/135‑1 (MQ=255)
                                                                   gcCGGACACTGCATGCTCTCTTTCGGATGGCGGAATCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCAGCGGAAGTCGACCAGCATGACATTGAcc                                                                    <  1:296839/135‑1 (MQ=255)
                                                                       gCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACAt                                                                <  5:259498/135‑1 (MQ=255)
                                                                              cATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTgc                                                          <  3:96191/134‑1 (MQ=255)
                                                                              cATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACAAATACGGTGATTGCACCGAGGGTAGCGAAGCCGCGGAAGTCGACAAGCATGACATTGACCACATTGTTgc                                                          <  3:294461/134‑1 (MQ=255)
                                                                               aTGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTgc                                                          <  7:329967/133‑1 (MQ=255)
                                                                                    ctctTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGccc                                                  >  4:193249/1‑136 (MQ=255)
                                                                                         tCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACCGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAg                                              >  2:145844/1‑135 (MQ=255)
                                                                                             aGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAAt                                         <  3:93197/136‑1 (MQ=255)
                                                                                              gggCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAAt                                         <  7:267365/135‑1 (MQ=255)
                                                                                              gggCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATg                                        <  4:233409/136‑1 (MQ=255)
                                                                                                gCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATgcg                                      <  4:323897/136‑1 (MQ=255)
                                                                                                  ggAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATgcgc                                     <  5:175441/135‑1 (MQ=255)
                                                                                                  ggAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATgcgc                                     <  6:229458/135‑1 (MQ=255)
                                                                                                    aaGCGCCGTAGCAGGGCTAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTAGCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCgg                                   <  7:162101/135‑1 (MQ=255)
                                                                                                    aaGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCgg                                   >  2:319767/1‑135 (MQ=255)
                                                                                                    aaGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGc                                  >  4:325747/1‑136 (MQ=255)
                                                                                                       cgcCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTc                                <  1:147615/135‑1 (MQ=255)
                                                                                                            tAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCaaa                             >  3:348315/1‑133 (MQ=255)
                                                                                                            tAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATa                           <  1:104503/135‑1 (MQ=255)
                                                                                                            tAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATa                           <  8:191003/135‑1 (MQ=255)
                                                                                                             aGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAg                          >  3:87022/1‑135 (MQ=255)
                                                                                                                     gAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAgg                   >  1:100478/1‑134 (MQ=255)
                                                                                                                       acacGGTCAATGCCTCAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTgc                                                          <  6:296201/93‑1 (MQ=255)
                                                                                                                       acacGGTCAATGCCTCAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTgc                                                          >  5:296201/1‑93 (MQ=255)
                                                                                                                       acacGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGaaa                >  1:37739/1‑135 (MQ=255)
                                                                                                                       acacGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAAt               >  4:147307/1‑136 (MQ=255)
                                                                                                                       acacGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAAt               >  4:318520/1‑136 (MQ=255)
                                                                                                                         acGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATGt             >  3:196308/1‑136 (MQ=255)
                                                                                                                         acGGGCAATGCCACAGCCACCAATACGGTGAGTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGGCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATg              <  5:112693/135‑1 (MQ=255)
                                                                                                                               aaTGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATGTCGTTgg       >  4:174799/1‑136 (MQ=255)
                                                                                                                               aaTGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGCTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATGTCGTTg        >  8:331/1‑135 (MQ=255)
                                                                                                                                aTGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGACGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATGTCGTTgg       <  1:335085/135‑1 (MQ=255)
                                                                                                                                     acaGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATGTCGTTGGGCgtc  <  7:113523/135‑1 (MQ=255)
                                                                                                                                     acaGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATGTCGTTGGGCgtc  <  2:149488/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GGTATCAGTGGCATGCCGCGGGTTGATCAGGTCGGTGACCACGTCTGGCGCCAACTGGCGTTGTTGCGCCGGCAACTGCATGCTCTCTTTCGGAGGGCGGAAGCGCCGTAGCAGGGCGAACACGGTCAATGCCACAGCCACCAATACGGTGATTTCACCGAGGGTATCGAAGCCGCGGAAGTCGACCAGCATGACATTGACCACATTGGTGCCGCCGCCCTGAGGCAATGCGCGGCTCAAATAGAACGAGGAAATGTCGTTGGGCGTC  >  KT2440/2529657‑2529924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: