Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,569,872 A→C 27.8% L518R (CTG→CGG)  acoR_3 ← Acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,569,8720AC27.8% 60.5 / 4.0 55L518R (CTG→CGG) acoR_3Acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base A (31/6);  new base C (3/14);  total (34/21)
Fisher's exact test for biased strand distribution p-value = 5.11e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACAGGTTGCCATTGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCGGGCAT  >  KT2440/2569739‑2569998
                                                                                                                                      |                                                                                                                              
cgACAGGTTGCCATTGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAgg                                                                                                                              >  4:282162/1‑136 (MQ=255)
    tgtttGCCTTTGGCTTGCGCTCCCCGGCGCTGCAGGTCCGTCTCTGCCGTCGCTCTCGGCACCGTTGTTTTCGCCTCGGCAAGAACCCCCGCTGCAAGGTGCGCGACGCCGACGGGCCCGTTGG‑CCGCCAGGGca                                                                                                                           <  2:26341/132‑2 (MQ=255)
            aTTGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGAcc                                                                                                                  >  4:106007/1‑136 (MQ=255)
                        gACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGCCCTGTAGCACGCt                                                                                                       >  4:248145/1‑135 (MQ=255)
                        gACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCCCGCt                                                                                                       >  7:74878/1‑135 (MQ=255)
                        gACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGc                                                                                                        >  8:246700/1‑134 (MQ=255)
                             ggCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTTG‑CCGCCACGGCCCACCCCGCCGGTATCCCGGTCACCa                                                                                                  >  7:321691/1‑135 (MQ=255)
                                   gCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGCCGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTcgc                                                                                            >  7:127319/1‑135 (MQ=255)
                                     aGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTagc                                                                                            >  5:168674/1‑133 (MQ=255)
                                     aGGTCCGTCTCTTCAGTAGCTATAGGCACCGGTGTTTTCGACTCGGCCAGAAATCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCCCGGCCAACGCCCCCGGTATCACGCTCCCGAGTTcgc                                                                                            >  8:215967/1‑133 (MQ=255)
                                       gTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAgg                                                                                       >  4:190517/1‑136 (MQ=255)
                                          cGTCTCTTCCGTCGCTAGCGCCACGGGTGTTTGCGACGCGCCCACAACGCCCGCGGCACGGGGCTCGCCGCCGACCGCCCCGTTGG‑CCGCCCGGGCAAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGtt                                                                                     <  5:147707/135‑1 (MQ=255)
                                           gTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCgcgcccggtg                                                                                    >  8:63617/1‑129 (MQ=255)
                                            tctcTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGc                                                                                   >  2:269254/1‑135 (MQ=255)
                                             ctctTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGCCCGGCCCGTTGG‑CCGCCACGGCCAACGCGACCCGTAGCCCGCTCACCAGTTAGCGCAGGGTGccc                                                                                 >  3:37764/1‑136 (MQ=255)
                                               ctTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCg                                                                                >  7:291317/1‑135 (MQ=255)
                                               ctTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCg                                                                                >  8:23028/1‑135 (MQ=255)
                                                   aggcGCTAGCGGCACCGGTGTTTGCGACCCGCCCACAACGCCCGCTGCACGGTGCGCGACGCCCCCCGGCCCGTTGG‑CCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCgg                                                                               <  8:55635/129‑1 (MQ=255)
                                                           tCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCCACCCGCCCTGTAGCACGCTCAACAGTTCGCGCAGGTTTCCCGGCCCGGGgtggt                                                                    >  1:83471/1‑133 (MQ=255)
                                                           tCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGgtgt                                                                     >  7:253943/1‑134 (MQ=255)
                                                             ggCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGAt                                                                 >  4:159951/1‑136 (MQ=255)
                                                               cACCGGGTTTTTCTACTCGGCCAGAAAGCCCGGTGGAAGTGGCTCGACGCCCA‑CGGGCCGTTGGCCCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATc                                                                <  6:189838/135‑1 (MQ=255)
                                                                   ggggtTTGCGCCCCGCCCAGATAGCCCGCTGGACGGGGCGCGACGCCGCCCGGCCCGTGGG‑CCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAAc                                                            <  7:158850/132‑1 (MQ=255)
                                                                    gtgtTTTAGAATCGCCCATAATCCCCGCTGCAAGGTGCGCTACGCCGACCGGCCCTTTGG‑CCGCCAGGCCAAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACa                                                           <  6:168674/135‑1 (MQ=255)
                                                                      gtTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCCACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAgg                                                         >  5:189838/1‑135 (MQ=255)
                                                                        tttCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGCCCTGTAGCACGCTCACCAGGTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTc                                                       >  3:40360/1‑135 (MQ=255)
                                                                            gACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGCCCTGTAGCACGCTCACCAGTTCGCGCACGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATa                                                   >  6:353023/1‑135 (MQ=255)
                                                                            gACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCCCCAGGGCCAACGCGACCTGGAGCACGCTCACCAGTTAGCGCAGGTTGCCCGGCCAGGGGGGTTGATCGAACACGTCGATa                                                   >  7:357951/1‑135 (MQ=255)
                                                                                    cAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGGCAACGCGCCCGGTAGCACGCTCACCAGTTCGCGCAGGGTGCCCGGCCAGGGGGGTTGATCGCACAGGTCGCTTATGGGcgg                                           >  5:74733/1‑135 (MQ=255)
                                                                                    cAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCcgg                                           >  2:12874/1‑135 (MQ=255)
                                                                                               cgggATGGTGCCCGACGCCGCCCGGCCGGTTGG‑CCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCgcgc                               <  3:221246/134‑1 (MQ=255)
                                                                                                tGGAAGGTGCTCGACGCCGACCGGCCCGTTTG‑CCGCCAGGGCCCACGCGACCTGTAGCCCGCTAACCAGGTAGCGCAGGTTGCCCGGCCAGGGGTGGTGATCGGACAGGTCGATAAAGGCCGGCGGCAGCCgcgc                               >  6:128451/1‑135 (MQ=255)
                                                                                                tGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCCGGGCCAACGCGACCGGTAGCACGCACACCAGTTCGCGCAGGTTGCCCGGCCCGGGGGGTTGTGCGACCAGGTCGATAATGGCCCGCGGCAGCCgcgc                               >  5:25023/1‑135 (MQ=255)
                                                                                                gggACGGGGCGCGCCGCCGACCGGCCCGGGGG‑CCGCCAGGCCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCgcgc                               <  1:28614/134‑1 (MQ=255)
                                                                                                        gCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGCCCGGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCCGGGGGGTTGATCGAACAGGTCGAATATGGCCGGCGGCAGCCGCGCAGGGTGccc                      >  3:48074/1‑136 (MQ=255)
                                                                                                          gcgccgccgcCGGGCCCGTGGC‑CCGCCCGGCCAAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCg                     <  2:67842/131‑1 (MQ=255)
                                                                                                            gACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCggg                   >  5:86255/1‑135 (MQ=255)
                                                                                                             aCGCCTCCCGGCCCGGTGG‑CCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGc                  <  2:83471/135‑1 (MQ=255)
                                                                                                              cGCCGACCGGCCGGTGGC‑CCGCCCGGCCAAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTc                <  3:124581/136‑1 (MQ=255)
                                                                                                                   cccGGCCCGGTGG‑CCGCCCGGGCAAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGt           <  3:248145/135‑1 (MQ=255)
                                                                                                                   acgggCCCGTGGC‑CCGCCCGGCCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGGTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCgg            <  5:173740/132‑1 (MQ=255)
                                                                                                                   aCCGGCCGGGTGG‑CGGCGTGGGCCACCGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGGTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGt           <  3:107890/136‑1 (MQ=255)
                                                                                                                   aCCGGCCCGTGGG‑CCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCCGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCgg            <  2:90302/135‑1 (MQ=255)
                                                                                                                    ccGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCCGCCAGGGGTGTTGATAGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGCGCTCGCgg            >  4:307477/1‑134 (MQ=255)
                                                                                                                    ccGGCCCGTGGC‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGt           <  7:54212/135‑1 (MQ=255)
                                                                                                                      ggCCCGTTGG‑CCGCCCGGGCCAACGCGCCCTGTAGCCCGCGCACCAGCTCGCGCCGGTTGCCCGGCCCGGGGGGTTGATCGAACCGGTCGCGACTGGCCGGCGGCAGCCGCGCAGGTGGGCCGGGCGCGCggggg         >  7:333021/1‑132 (MQ=255)
                                                                                                                      ggCCCGTTGG‑CCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGt                                                                                      <  8:225265/58‑1 (MQ=255)
                                                                                                                      ggCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCCGGTTGCCCGGCCAGGGGTGTTGATCGCACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGc        >  3:236669/1‑136 (MQ=255)
                                                                                                                      ggCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGt                                                                                      >  7:225265/1‑58 (MQ=255)
                                                                                                                       gCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGc        <  1:269254/135‑1 (MQ=255)
                                                                                                                        cccGGTGC‑CCGCCCGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCgg      <  3:282162/136‑1 (MQ=255)
                                                                                                                           gTTGG‑CCGCCAGGGCCCACGCGCCCTGTAGCACGCTCCCCAGGTCGCGCAGGTTGCCCGGCCAGGGGTGGTGATCGAACAGGGCGATAATGGCCGGCGGCAGCCGCGCCGGTTGCCCGGGCGCGCGGTAGCGGGcc   >  3:73230/1‑135 (MQ=255)
                                                                                                                           gTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCGGGc    >  8:269139/1‑135 (MQ=255)
                                                                                                                            ttGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCGGGCAt  <  4:144118/136‑1 (MQ=255)
                                                                                                                             tGG‑CCGCCCGGGCCACCGCGACCTGTAGCACGCTCACCAGTGCGCGCAGGGTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCGGGCAt  <  2:159895/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                              
CGACAGGTTGCCATTGGCTTGCTCGACCAGGCTCTGCAGGTCCGTCTCTTCAGTCGCTATCGGCACCGGTGTTTTCGACTCGGCCAGAAAGCCCGCTGGAAGGTGCTCGACGCCGACCGGCCCGTTGG‑CCGCCAGGGCCAACGCGACCTGTAGCACGCTCACCAGTTCGCGCAGGTTGCCCGGCCAGGGGTGTTGATCGAACAGGTCGATAATGGCCGGCGGCAGCCGCGCAGGTTGCCCGGGCTCGCGGTAGCGGGCAT  >  KT2440/2569739‑2569998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑