Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,571,359 G→A 5.5% H22H (CAC→CAT)  acoR_3 ← Acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,571,3590GA 5.5% 239.3 / 3.2 73H22H (CAC→CAT) acoR_3Acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (28/41);  new base A (2/2);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

CCAAGAGGTGCGCATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCAGTGTTGT  >  KT2440/2571226‑2571475
                                                                                                                                     |                                                                                                                    
ccAAGAGGTGCGCATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGt                                                                                                                     >  2:336078/1‑135 (MQ=255)
       gTGCGCATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCgg                                                                                                             <  3:355446/136‑1 (MQ=255)
       gTGCGCATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCgg                                                                                                             <  4:47490/136‑1 (MQ=255)
        tGCGCATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCgg                                                                                                             <  8:43504/135‑1 (MQ=255)
        tGCGCATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCgg                                                                                                             <  8:41294/135‑1 (MQ=255)
          cgcATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCa                                                                                                           >  1:182162/1‑135 (MQ=255)
            cATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCg                                                                                                        >  4:180516/1‑136 (MQ=255)
             aTAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCgg                                                                                                             >  2:75124/1‑130 (MQ=255)
             aTAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCgg                                                                                                       >  4:192802/1‑136 (MQ=255)
             aTAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCg                                                                                                        >  7:316478/1‑135 (MQ=255)
             aTAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCg                                                                                                        >  7:116819/1‑135 (MQ=255)
             aTAGTGCGGCAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCgg                                                                                                             <  1:75124/130‑1 (MQ=255)
                  gCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTc                                                                                                     >  7:153474/1‑133 (MQ=255)
                    ggTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAg                                                                                                 >  1:339970/1‑135 (MQ=255)
                    ggTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAg                                                                                                 >  3:116419/1‑135 (MQ=255)
                      tAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGa                                                                                               >  7:137033/1‑135 (MQ=255)
                        gCACTTCCTCGCGGGTCAGGCGCAGCAGGTGTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGc                                                                                            <  4:244969/136‑1 (MQ=255)
                         cACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGc                                                                                            >  8:64720/1‑135 (MQ=255)
                           cTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTg                                                                                         <  3:180516/136‑1 (MQ=255)
                            ttCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCGAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTg                                                                                         <  1:316497/135‑1 (MQ=255)
                              ccTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGAc                                                                                              <  5:25140/128‑1 (MQ=255)
                              ccTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGAc                                                                                              >  6:25140/1‑128 (MQ=255)
                              ccTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGa                                                                                       >  5:151176/1‑135 (MQ=255)
                              ccTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGAt                                                                                      >  4:253965/1‑136 (MQ=255)
                               cTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCTGTCCCAGGACGCCTGGATc                                                                                     <  3:275172/136‑1 (MQ=255)
                                     ggTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTgg                                                                               <  3:186433/136‑1 (MQ=255)
                                      gTCAGGCGCAGCAGGGTGTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCAGGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTgg                                                                               <  6:207359/135‑1 (MQ=255)
                                         aGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATc                                                                                     <  5:153039/126‑1 (MQ=255)
                                         aGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATc                                                                                     >  6:153039/1‑126 (MQ=255)
                                             gcagcaGGTTTTGGCGCCGCTCCAGCAGGGCCCTGAGATCGGCGCGGGACAGGCAGGCAAAGTCTGGCGCATGCTGCGGCTGCCGGCCGGGGGCGCGGCCGCGGTCCCCGGACGCCGGGATCCGTTGGGtgtgcg                                                                        >  6:55380/1‑133 (MQ=255)
                                                   ggTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAg                                                                  >  1:280036/1‑135 (MQ=255)
                                                       ttGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCTTGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCCGGATCAGTTGGGTGTGGGTCGAAGCGGa                                                              <  7:358600/135‑1 (MQ=255)
                                                       tgggCGCCGCTCCCGCAGGGCACGGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCGGCGGCTGCAGGGCGGGGGCGCGGCAGCGGGCCCAGGCCGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGa                                                              <  5:342896/133‑1 (MQ=255)
                                                         ggCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATc                                                                                     <  4:227220/110‑1 (MQ=255)
                                                         ggCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATc                                                                                     >  3:227220/1‑110 (MQ=255)
                                                         ggCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGc                                                           >  3:124938/1‑136 (MQ=255)
                                                           ccccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGGCAAAGGCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGACGCGGATGCgg                                                         <  3:10671/134‑1 (MQ=255)
                                                            gccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  6:94079/135‑1 (MQ=255)
                                                            gccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  2:339970/135‑1 (MQ=255)
                                                            gccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  1:296464/135‑1 (MQ=255)
                                                            gccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  8:137033/135‑1 (MQ=255)
                                                            gccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  6:183802/135‑1 (MQ=255)
                                                            gccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  8:316478/135‑1 (MQ=255)
                                                            gccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  5:305693/135‑1 (MQ=255)
                                                            gccgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGGGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  8:362368/135‑1 (MQ=255)
                                                            gccgcTCCAGCACGGCACTGAGCGCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         <  7:246185/135‑1 (MQ=255)
                                                              cgcTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCgg                                                         >  1:141018/1‑133 (MQ=255)
                                                                 tCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATg                                                    <  1:21785/135‑1 (MQ=255)
                                                                   cagcagGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATagg                                                  <  1:41436/135‑1 (MQ=255)
                                                                     gcagGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGcct                                               <  3:328780/136‑1 (MQ=255)
                                                                     gcagGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGcct                                               <  4:116419/136‑1 (MQ=255)
                                                                         ggCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGcctgtc                                            <  8:116819/135‑1 (MQ=255)
                                                                              tGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCtgtg                                       >  1:154151/1‑135 (MQ=255)
                                                                                aGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGGCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGa                                    <  4:52591/136‑1 (MQ=255)
                                                                                 gCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGa                                    <  2:280036/135‑1 (MQ=255)
                                                                                        gcgGGCCAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGAttgttg                              >  5:144269/1‑134 (MQ=255)
                                                                                                  cAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGa                   <  6:151176/135‑1 (MQ=255)
                                                                                                            cTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCa         <  2:42897/135‑1 (MQ=255)
                                                                                                            cTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGGGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCa         <  2:227273/135‑1 (MQ=255)
                                                                                                                 gcATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCAGtgtt    <  8:153474/135‑1 (MQ=255)
                                                                                                                  cATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGa                                    >  7:272611/1‑102 (MQ=255)
                                                                                                                  cATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGa                                    <  8:272611/102‑1 (MQ=255)
                                                                                                                  cATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCAGtgttgt  >  3:249412/1‑136 (MQ=255)
                                                                                                                  cATGCTGCGGCTGCAGGCCATGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGcct                                               <  5:172574/91‑1 (MQ=255)
                                                                                                                  cATGCTGCGGCTGCAGGCCATGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGcct                                               <  5:179006/91‑1 (MQ=255)
                                                                                                                  cATGCTGCGGCTGCAGGCCATGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGcct                                               >  6:179006/1‑91 (MQ=255)
                                                                                                                  cATGCTGCGGCTGCAGGCCATGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGcct                                               >  6:172574/1‑91 (MQ=255)
                                                                                                                        gcGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTg                                                                                         >  6:197170/1‑43 (MQ=255)
                                                                                                                        gcGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTg                                                                                         <  5:197170/43‑1 (MQ=255)
                                                                                                                                  gCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCa         <  3:1283/113‑1 (MQ=255)
                                                                                                                                  gCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCa         <  3:129264/113‑1 (MQ=255)
                                                                                                                                  gCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCa         >  4:1283/1‑113 (MQ=255)
                                                                                                                                  gCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCa         >  4:129264/1‑113 (MQ=255)
                                                                                                                                     |                                                                                                                    
CCAAGAGGTGCGCATAGTGCGGTAGCACTTCCTCGCGGGTCAGGCGCAGCAGGTTTTGGCGCCGCTCCAGCAGGGCACTGAGCTCGGCGCGGGACAGGCAGTCAAAGTCTGGCGCATGCTGCGGCTGCAGGCCGTGGGCGCGGCAGCGGTCCCAGGACGCCTGGATCAGTTGGGTGTGGGTCGAAGCGGATGCGGCCATGGGCCTGTCCTGTGTGATTGTTGTCATGGGTCGACCGCCGTTCAGTGTTGT  >  KT2440/2571226‑2571475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑