Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,607,430	G→T	5.8%	Q1104H (CAG→CAT)	CJ019_02463 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,607,430	0	G	T	5.8%	198.4 / 4.1	69	Q1104H (CAG→CAT)	CJ019_02463	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (43/21);  new base T (2/2);  total (46/23)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01

CGATGGGACGAAGAGCGCTGTGTCCCTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTCCCGATGGAG  >  KT2440/2607298‑2607562                                                                                                                                     |                                                                                                                                     cGATGGGACGAAGAGCGCTGTGTCCCTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCCTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAgg                                                                                                                                     <  1:258070/134‑1 (MQ=255)         cGAAGAGCGCTGTGTCCCTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGaaa                                                                                                                            <  7:92825/135‑1 (MQ=255)          gAAGAGCGCTGTGTCCCTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGaaaa                                                                                                                           >  8:68506/1‑135 (MQ=255)          gAAGAGCGCTGTGTCCCTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAg                                                                                                                          >  3:352155/1‑136 (MQ=255)                   tgtgTCCCTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCcatcat                                                                                                                  <  6:188420/135‑1 (MQ=255)                        cccTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCCTGGCCCTTGCAAAGCACCTGGACGTGTACAACGGGCACGACATCGAAAAGGCAAACATGgccgc                                                                                                            >  3:344326/1‑136 (MQ=255)                        cccTCAACGACTTCATCCAAACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGCGGCAGGACATCGAAAAGGCCATCATGgccg                                                                                                             >  1:306974/1‑135 (MQ=255)                          cTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGCCATCGAAAAGGCCATCATGgccgcc                                                                                                           >  5:191866/1‑135 (MQ=255)                           tCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGgccgcccc                                                                                                         >  3:132301/1‑134 (MQ=255)                                gACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTga                                                                                                      >  5:106230/1‑134 (MQ=255)                                gACTTCATCCATAACAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTTTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTgag                                                                                                     <  7:360888/135‑1 (MQ=255)                                    tCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGaa                                                                                                   >  8:130203/1‑133 (MQ=255)                                     cATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACaa                                                                                                >  2:299997/1‑135 (MQ=255)                                        ccATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCATGACATCGAAAAGGCCATCATGGCCGCCACTgag                                                                                                     >  7:363215/1‑127 (MQ=255)                                        ccATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCATGACATCGAAAAGGCCATCATGGCCGCCACTgag                                                                                                     >  7:339092/1‑127 (MQ=255)                                        ccATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCATGACATCGAAAAGGCCATCATGGCCGCCACTgag                                                                                                     <  8:339092/127‑1 (MQ=255)                                        ccATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCATGACATCGAAAAGGCCATCATGGCCGCCACTgag                                                                                                     <  8:363215/127‑1 (MQ=255)                                         cATAACAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGTCCGCCACTGAGAACAAGTTCg                                                                                           <  4:122672/136‑1 (MQ=255)                                          aTACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGCCGTGTACACGGGGCAGGCCATCGAAAAGGCCATCATGGCCGCCACTGAGCACAAGTTct                                                                                           >  1:321928/1‑134 (MQ=255)                                          aTACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCgg                                                                                          <  4:213063/136‑1 (MQ=255)                                          aTACCAACGCCTTCGAAGTAGTGGAACCGTACATGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCCCTGAGAACAAGttat                                                                                           >  2:268302/1‑133 (MQ=255)                                                   ccTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGa                                                                                  >  1:76655/1‑135 (MQ=255)                                                   ccTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGAt                                                                                 >  3:167175/1‑136 (MQ=255)                                                    cTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAACAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGAt                                                                                 >  7:32648/1‑135 (MQ=255)                                                    cTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGAt                                                                                 >  5:208596/1‑135 (MQ=255)                                                    cTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGAt                                                                                 >  8:296360/1‑135 (MQ=255)                                                         aaGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGccc                                                                           >  4:198357/1‑136 (MQ=255)                                                          agtagtGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGGACAAGGGGCAGGACATCGAAAAGGCCATCCTGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCa                                                                          <  4:344592/136‑1 (MQ=255)                                                            tagtGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAACGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAg                                                                         <  1:299997/135‑1 (MQ=255)                                                               tGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACACGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGgc                                                                       <  7:259708/134‑1 (MQ=255)                                                               tGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGTCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGgcgc                                                                     <  4:325127/136‑1 (MQ=255)                                                               tGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGgcg                                                                      >  8:52738/1‑135 (MQ=255)                                                                  aaCCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGAGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGa                                                                   <  6:208596/135‑1 (MQ=255)                                                                         ccTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGc                                                            >  8:346868/1‑135 (MQ=255)                                                                          cTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGTGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAACTTCGGGGAAGGGATGCGCCCAGGCGCGAACATGGcc                                                           >  2:189495/1‑135 (MQ=255)                                                                          cTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCg                                                          >  4:40966/1‑136 (MQ=255)                                                                          cTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGCACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCAAGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGCACTTgcac                                                           >  7:298187/1‑132 (MQ=255)                                                                            gcgcCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCCTCATGGCCGCCACTGAGAACAAGTTCGGGGACGGGATGCGCCCAGGCGCGAACGTGGCCg                                                          >  7:369341/1‑134 (MQ=255)                                                                               ccGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATg                                                     >  3:175887/1‑136 (MQ=255)                                                                               ccGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAACGCCATCATGGCCGCCACAGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTAGGCCGGTAt                                                      >  5:230202/1‑135 (MQ=255)                                                                                cGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATg                                                     >  1:26031/1‑135 (MQ=255)                                                                                cGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGc                                                    <  3:306912/136‑1 (MQ=255)                                                                                cGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGc                                                    >  3:244350/1‑136 (MQ=255)                                                                                     ggCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGaaa                                                >  8:218531/1‑135 (MQ=255)                                                                                          ggAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCCTCATGGCCGCCACTGCGAACAAGTGCGGGGAAGGGATGCGCCCAGGCGCGCACTTGGCCGGTAGGCGAAAGGCCtt                                          >  3:341007/1‑136 (MQ=255)                                                                                             gCGTGGCCCGTGCAAAGCACCGGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGTAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGa                                        <  7:141810/135‑1 (MQ=255)                                                                                               gTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGCTGCGCCCAGGCGCGGACTTGGCCGGTATGCGAAAGGACTTGAAg                                      >  1:331075/1‑135 (MQ=255)                                                                                               gTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAg                                                                         <  1:287242/100‑1 (MQ=255)                                                                                               gTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAg                                                                         >  2:287242/1‑100 (MQ=255)                                                                                               gTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAg                                      >  7:146735/1‑135 (MQ=255)                                                                                               gTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAg                                      >  1:73117/1‑135 (MQ=255)                                                                                               gTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGt                                     >  4:320014/1‑136 (MQ=255)                                                                                               gTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGt                                     >  4:319207/1‑136 (MQ=255)                                                                                                         cAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTc                             <  7:346868/134‑1 (MQ=255)                                                                                                          aaaGCACGTGGACGTGTCCAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCg                            <  6:230202/134‑1 (MQ=255)                                                                                                          aaaGCACCTGGACGTGTACAAGGGGCTGGACATCGAAAAGCCCATCATGGCCGCCACTGAGAACAAGATCGGGGACGGGATGCGCCCAGGCGCGAAGTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCg                            <  2:72851/134‑1 (MQ=255)                                                                                                          aaaGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGAc                          >  4:337621/1‑136 (MQ=255)                                                                                                                 cTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATcc                   <  3:40966/136‑1 (MQ=255)                                                                                                                  tGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCAt                     >  1:66784/1‑133 (MQ=255)                                                                                                                    gACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCAGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTAGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAg                 >  1:47286/1‑135 (MQ=255)                                                                                                                           aCAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTcc          >  2:89751/1‑135 (MQ=255)                                                                                                                           aCAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTcc          >  5:129229/1‑135 (MQ=255)                                                                                                                           aCAAGGGGCAGGACATCGAAAAGGCCATCAAGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTccc         >  4:221776/1‑136 (MQ=255)                                                                                                                            cAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTcc          >  2:19298/1‑134 (MQ=255)                                                                                                                            cAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTcc          >  1:231164/1‑134 (MQ=255)                                                                                                                                gggCAGGACATCGAAACGGCCATCATGGCCGCCACTTAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCTGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTCCCGATg     <  5:176443/135‑1 (MQ=255)                                                                                                                                gggCAGCACATCGAAACGGCCAGCCTGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTCCCGATg     <  5:219481/135‑1 (MQ=255)                                                                                                                                  gCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTCCCGATggag  <  4:343507/136‑1 (MQ=255)                                                                                                                                  gCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTCCCGATgga   <  2:231164/135‑1 (MQ=255)                                                                                                                                  gCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTCCCGATgga   >  1:240320/1‑135 (MQ=255)                                                                                                                                     ggACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGc                                 <  8:221568/102‑1 (MQ=255)                                                                                                                                     ggACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGc                                 >  7:221568/1‑102 (MQ=255)                                                                                                                                     |                                                                                                                                     CGATGGGACGAAGAGCGCTGTGTCCCTCAACGACTTCATCCATACCAACGCCTTCGAAGTAGTGGAACCGTACCTGCGCCGTATGGCAGGGAGCGTGGCCCTTGCAAAGCACCTGGACGTGTACAAGGGGCAGGACATCGAAAAGGCCATCATGGCCGCCACTGAGAACAAGTTCGGGGAAGGGATGCGCCCAGGCGCGAACTTGGCCGGTATGCGAAAGGACTTGAAGTTCGCCTTCGACCGCATCCAGGGCCTCCCGATGGAG  >  KT2440/2607298‑2607562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑