Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,671,714 A→G 9.7% D333G (GAC→GGC)  sasA_7 → Adaptive‑response sensory‑kinase SasA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,671,7140AG 9.7% 115.6 / 4.4 42D333G (GAC→GGC) sasA_7Adaptive‑response sensory‑kinase SasA
Reads supporting (aligned to +/- strand):  ref base A (19/18);  new base G (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

ATGCGCTGCCGGGTGCGCAGTTCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGACGATGGCCCGGGTAT  >  KT2440/2671585‑2671849
                                                                                                                                 |                                                                                                                                       
aTGCGCTGCCGGGTGCGCAGTTCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACcg                                                                                                                                      >  6:233885/1‑133 (MQ=255)
 tGCGCTGCCGGGTGCGCAGTTCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGa                                                                                                                                   <  6:247446/135‑1 (MQ=255)
          gggTGCGCAGTTCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGCCCGCGAGGACGTTCTg                                                                                                                         >  4:276340/1‑136 (MQ=255)
           ggTGCGCAGTTCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATTATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTg                                                                                                                         <  7:276224/135‑1 (MQ=255)
             tGCGCAGTTCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGaa                                                                                                                      >  3:173333/1‑136 (MQ=255)
              gcgcAGTTCGATTGTGATGCCGAACTGCGGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGaa                                                                                                                      <  6:291938/135‑1 (MQ=255)
                    ttCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACTTTCTGGAACTGTTgg                                                                                                               <  4:338314/136‑1 (MQ=255)
                     tCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGATCTGTTgg                                                                                                               <  3:288764/135‑1 (MQ=255)
                     tCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTgg                                                                                                               <  7:276290/135‑1 (MQ=255)
                          tgtgATGCCGATCTGCCGGGGTTGCTCGGTACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCGCGGGCTGTTGTTGCCATTGGACCGCGATGACGTTCTGGAACTGTTGGGTAAt                                                                                                          <  7:52207/135‑1 (MQ=255)
                          tgtgATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAAt                                                                                                          <  8:264866/135‑1 (MQ=255)
                                 ccGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTgg                                                                                                   >  6:220796/1‑135 (MQ=255)
                                 ccGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGa                                                                                                  >  3:129267/1‑136 (MQ=255)
                                                    cGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGc                                                                                >  1:168104/1‑135 (MQ=255)
                                                               gCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAgg                                                                     >  2:631/1‑135 (MQ=255)
                                                               gCAAGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACACCGCCTGCAAGTGGGCTGACAGCGAGGt                                                                    >  4:68992/1‑136 (MQ=255)
                                                                      tCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGGCCGCGa                                                                                                                                   >  6:45428/1‑66 (MQ=255)
                                                                      tCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGGCCGCGa                                                                                                                                   <  5:50630/66‑1 (MQ=255)
                                                                      tCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGGCCGCGa                                                                                                                                   <  5:45428/66‑1 (MQ=255)
                                                                      tCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGGCCGCGa                                                                                                                                   >  6:50630/1‑66 (MQ=255)
                                                                      tCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGt                                                              >  5:168659/1‑135 (MQ=255)
                                                                      tCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGt                                                              >  5:61936/1‑135 (MQ=255)
                                                                                ggCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATcgcg                                                    <  2:168104/135‑1 (MQ=255)
                                                                                 gCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGt                                                   >  5:153992/1‑135 (MQ=255)
                                                                                 gCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTc                                                  >  4:209320/1‑136 (MQ=255)
                                                                                   ctgctgctgGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTgg                                                                                                               <  6:257211/73‑1 (MQ=255)
                                                                                   ctgctgctgGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTgg                                                                                                               >  5:257211/1‑73 (MQ=255)
                                                                                   ctgctgctgGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCg                                                 >  3:256459/1‑135 (MQ=255)
                                                                                         ctgGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGc                                            <  4:122457/134‑1 (MQ=255)
                                                                                          gggCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGc                                            <  2:140149/132‑1 (MQ=255)
                                                                                              aaGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAggg                                      >  8:286204/1‑135 (MQ=255)
                                                                                               agggtggtgtCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGGTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGc                                     <  7:354934/129‑1 (MQ=255)
                                                                                                 tGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTa                                   >  2:148006/1‑135 (MQ=255)
                                                                                                             cGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGt                       <  5:233885/135‑1 (MQ=255)
                                                                                                              gggCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTc                      >  4:127631/1‑135 (MQ=255)
                                                                                                                  ggttgttGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAAGCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGGGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATg                  <  2:352648/134‑1 (MQ=255)
                                                                                                                   gttgttGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGAc                <  3:344555/136‑1 (MQ=255)
                                                                                                                       ttgCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGt                                                          <  5:306174/90‑1 (MQ=255)
                                                                                                                       ttgCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGt                                                          >  6:306174/1‑90 (MQ=255)
                                                                                                                          ccATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGACGATGGcc         <  4:173333/136‑1 (MQ=255)
                                                                                                                            aTGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGACGATGGccc        >  1:94498/1‑135 (MQ=255)
                                                                                                                             tGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGACGATGGCCCg       >  5:79633/1‑135 (MQ=255)
                                                                                                                                 aCCGCGAGTACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGACGATGGCCCGGGTAt  >  3:2242/1‑136 (MQ=255)
                                                                                                                                 aCCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGACGATGGCCCGGGTa   >  5:103754/1‑135 (MQ=255)
                                                                                                                                 aCCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGACGATGGCCCGGGTa   >  7:298551/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                                       
ATGCGCTGCCGGGTGCGCAGTTCGATTGTGATGCCGAACTGCCGGGGTTGCTCGGGACCTTGGGCATGATTCATGGCGAGGGCCTGCTGCTGGCAAGTGATGTGTCGCCCGGGCTGTTGTTGCCATGGGACCGCGAGGACGTTCTGGAACTGTTGGGTAATCTGCTGGACAACGCCTGCAAGTGGGCTGACAGCGAGGTGCGCGTGGGTATCGCGTCGAACGCCGAGGGCTACCGGCTGTGGGTCGATGACGATGGCCCGGGTAT  >  KT2440/2671585‑2671849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑