| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,721,707 | C→T | 31.8% | S126S (TCC→TCT) | gcvA_7 → | Glycine cleavage system transcriptionalactivator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,721,707 | 0 | C | T | 31.8% | 83.9 / 56.8 | 67 | S126S (TCC→TCT) | gcvA_7 | Glycine cleavage system transcriptionalactivator |
| Reads supporting (aligned to +/- strand): ref base C (18/27); new base T (7/14); total (26/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01 | |||||||||||
GCGCACTGCTGCGTCAGGATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGATGAATGGCTGATCCCGGTA > KT2440/2721580‑2721839 | gcgcACTGCTGCGTCAGGATCGATACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAgtgtgt > 5:64919/1‑135 (MQ=255) gcACTGCTGCGTCAGTATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAgtgtgt < 2:245955/133‑1 (MQ=255) gcACTGCTGCGTCAGGATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTGACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAgtgtgt < 5:273078/133‑1 (MQ=255) gcACTGCTGCGTCAGGATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAgtgtgt < 7:351521/133‑1 (MQ=255) gcACTGCTGCGTCAGGATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAgtgtgt < 8:166835/133‑1 (MQ=255) gcACTGCTGCGTCAGGATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAgtgtgt < 6:81144/133‑1 (MQ=255) gcACTGCTGCGTCAGGATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAgtgtgt < 5:111686/133‑1 (MQ=255) gcACTGCTGCGTCAGGATAGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAgtgtgt < 2:126282/133‑1 (MQ=255) gcACTGATGCGTCAGGATCGCTACGGCCTGAGCCTTAAGGCGCCGTCCACGCTCACGTTGCGCTGGCTGCTGCGGGCGCTGTTTGCGTTCAAGAAGGTCGAGGACAACTGCAGTTTGCAGCTGTCTAgtgtgt < 5:330132/133‑1 (MQ=255) tgcGTCAGGATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGata > 2:316106/1‑135 (MQ=255) ggATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGc < 3:348977/136‑1 (MQ=255) ggATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGc < 3:68825/136‑1 (MQ=255) gagcGCTCCGGCCTGCGCCTGCCGGCGCCGTCCACTCTGACGGTGCGCTGGCTGCTGCGGGCGCTGGAGGCGTTCAAGAAGGTAGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGc < 2:355033/132‑1 (MQ=255) gATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGc < 6:134984/135‑1 (MQ=255) gATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGc < 7:87361/135‑1 (MQ=255) gATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGc < 6:64919/135‑1 (MQ=255) gATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGc < 8:270710/135‑1 (MQ=255) gATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGc < 5:163524/135‑1 (MQ=255) cGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGGTCAAGAAGGTCGCGGACCACGGCAGTGGGCAGCTGGCCAGTGTGTGGGTGGATTTCGGCAGCGGGGGttt > 5:337106/1‑135 (MQ=255) tGCGCCTGCAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGAGGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 5:99145/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTGACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGTTTTCTAtt < 7:191354/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 2:103582/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 6:166251/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 6:294693/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 7:41500/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 1:5343/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 8:263615/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 2:98557/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGAGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATTTCGACAGCGTGGATTTCTAtt < 8:359342/135‑1 (MQ=255) tGCGCCTGAAGGCGCCGTCCACGCTCACGGGGCTCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 6:133981/135‑1 (MQ=255) cgcCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTcc < 6:191261/135‑1 (MQ=255) ggCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTa < 2:268938/135‑1 (MQ=255) ggCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTa < 1:316106/135‑1 (MQ=255) gcgcCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGCTGCGTTGAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTAc > 2:47953/1‑135 (MQ=255) gcgcCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGCATGGATATCGACAGCCTGGAGTTCTATTCCGAGCCCttc > 3:152000/1‑133 (MQ=255) gcgcCGTCCACGCGCACGGCGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACg < 3:73385/136‑1 (MQ=255) ccACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATGGACAGCGTGGATTTCTATTCCGAGCCCTACGACtgtg > 5:97685/1‑135 (MQ=255) tGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt > 1:208530/1‑105 (MQ=255) tGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 2:208530/105‑1 (MQ=255) tGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTgg > 2:299004/1‑135 (MQ=255) tggctgCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACtgtg > 8:295060/1‑118 (MQ=255) tggctgCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACtgtg < 7:295060/118‑1 (MQ=255) ctgcGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACtgtg < 5:167479/112‑1 (MQ=255) ctgcGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACtgtg > 6:167479/1‑112 (MQ=255) tgcGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGa < 8:107911/134‑1 (MQ=255) gcGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGCtt > 8:38418/1‑135 (MQ=255) cTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCccgc > 7:131638/1‑134 (MQ=255) tGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGatat < 6:190774/135‑1 (MQ=255) aagaagGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt > 7:137634/1‑73 (MQ=255) aagaagGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 3:195106/73‑1 (MQ=255) aagaagGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt > 4:195106/1‑73 (MQ=255) aagaagGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTAtt < 8:137634/73‑1 (MQ=255) cGAGGACAACTGCATTGTGCAGCTGTCCAGTGTGTGTATTGATATAGACATCGTGGATTTCTATTCCGAGACCAACGACTGTGCCATTCTACTGGCCAGATTGCGATTTCCCGACGATATCGAGAGTTTCAAGCt < 6:335740/135‑1 (MQ=255) gAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGt > 4:11995/1‑136 (MQ=255) aGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTAc > 6:72638/1‑74 (MQ=255) aGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTAc < 5:72638/74‑1 (MQ=255) aCAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGa > 3:141104/1‑136 (MQ=255) gCAGTGTGCAGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGGTTCAATCTGTTCGATGTAt > 6:341321/1‑135 (MQ=255) cAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGATGAATg > 2:5094/1‑135 (MQ=255) aGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAg > 5:47460/1‑50 (MQ=255) aGCTGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAg < 6:47460/50‑1 (MQ=255) aGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCCGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGCTGAATGGCTGATcc > 2:76920/1‑135 (MQ=255) aGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGATGAATGGCTGATccc > 4:281268/1‑136 (MQ=255) aGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGATGAATGGCTGATcc > 7:9282/1‑135 (MQ=255) aGCTGTCAAGGGTGTGGATGGATATCGACACCGTCTAATTCTATGCCGAGACCTACTACTCTGCCAATCTCCTGGCCAGCGGGCGAGTTCAAGCCGATATCGAGAGATTCACGCTGTACGTTGAATGCCTGATacc > 4:253880/1‑136 (MQ=255) tGTCTAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGATGAATGGCTGATCCCg > 7:152432/1‑134 (MQ=255) tCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGATGAATGGCTGATCCCGGTa > 7:119563/1‑135 (MQ=255) | GCGCACTGCTGCGTCAGGATCGCTACGGCCTGCGCCTGAAGGCGCCGTCCACGCTCACGGTGCGCTGGCTGCTGCGGGCGCTGGATGCGTTCAAGAAGGTCGAGGACAACTGCAGTGTGCAGCTGTCCAGTGTGTGGATGGATATCGACAGCGTGGATTTCTATTCCGAGCCCTACGACTGTGCCATTCTCCTGGCCAGCGGGCGATTTCCCGCCGATATCGAGAGTTTCAAGCTGTTCGATGAATGGCTGATCCCGGTA > KT2440/2721580‑2721839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |