Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,911,037 C→T 8.7% Q2334* (CAG→TAG)  CJ019_02759 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,911,0370CT 8.7% 142.5 / 3.2 46Q2334* (CAG→TAG) CJ019_02759hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/22);  new base T (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTCACCGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAGCTCGGCCACCCACCT  >  KT2440/2910909‑2911152
                                                                                                                                |                                                                                                                   
cAGTCACCGGGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACg                                                                                                               <  5:149538/135‑1 (MQ=255)
 aGTCACCGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGc                                                                                                             <  4:167427/136‑1 (MQ=255)
  ggcACCGTGACCGGTGTCGATGCCATCGACCTGGGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGc                                                                                                             <  8:154874/133‑1 (MQ=255)
  gTCACCGTGACGGGGGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGc                                                                                                             <  1:133838/135‑1 (MQ=255)
      ccGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGCAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGCACCAGATGGAAAAGCTGCAGGACGGCGccc                                                                                                         >  6:265925/1‑135 (MQ=255)
      ccGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGccc                                                                                                         >  1:102071/1‑135 (MQ=255)
      ccGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAcc                                                                                                         >  7:144403/1‑135 (MQ=255)
      ccGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAc                                                                                                          <  5:349876/134‑1 (MQ=255)
      ccGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAc                                                                                                          <  5:195381/134‑1 (MQ=255)
      ccGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAc                                                                                                          <  2:270004/134‑1 (MQ=255)
      ccGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAc                                                                                                          <  7:75654/134‑1 (MQ=255)
      ccGTGACCGGAGTCGATGCCATCGACCTCTCGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCACCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAcc                                                                                                         >  5:267268/1‑135 (MQ=255)
       cgggACCGGGGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCg                                                                                                        <  2:187813/132‑1 (MQ=255)
          gACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCtt                                                                                                     <  7:14503/135‑1 (MQ=255)
            ccGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGACCCAGATGGAAAAACTGCAGGACGGCGAc                                                                                                          >  4:43114/1‑128 (MQ=255)
            ccGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAc                                                                                                          <  3:167833/128‑1 (MQ=255)
            ccGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAc                                                                                                          <  3:43114/128‑1 (MQ=255)
            ccGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGAc                                                                                                          >  4:167833/1‑128 (MQ=255)
                   cGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTCCTACCTg                                                                                           >  3:332152/1‑136 (MQ=255)
                    gATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCTGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCg                                                                                                        >  2:264175/1‑122 (MQ=255)
                    gATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCg                                                                                                        <  5:245618/122‑1 (MQ=255)
                    gATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCg                                                                                                        <  1:264175/122‑1 (MQ=255)
                    gATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCg                                                                                                        >  6:245618/1‑122 (MQ=255)
                                     gATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCt                                                                          >  5:127766/1‑135 (MQ=255)
                                      aTCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTg                                                                         >  2:246234/1‑135 (MQ=255)
                                      aTCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTg                                                                         >  1:169479/1‑135 (MQ=255)
                                            ggcCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGAt                                                                    >  6:136717/1‑134 (MQ=255)
                                             gcCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGTAGGACGGCGACCGCTTCTACTACCTGGAGCGCACCGCGGGCCTGTCGATGa                                                                  >  2:288014/1‑135 (MQ=255)
                                             gcCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGa                                                                  <  1:307870/135‑1 (MQ=255)
                                             gcCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATCCTCGGCAGCACCAACAACTTCGTCTTCGAGAACCAGATGCAAAAAATGTAGGACGCCCACCGCTACTACAACCTCGACCGCACACCGGGCCTGACCATGa                                                                  >  2:292912/1‑135 (MQ=255)
                                                           aaaGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTgg                                                      >  6:80345/1‑133 (MQ=255)
                                                                    tttGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGCACCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCg                                           >  2:310170/1‑135 (MQ=255)
                                                                      tGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAAATGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGtt                                         <  3:362615/135‑1 (MQ=255)
                                                                                 tCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCg                                                                                                        <  4:301054/61‑1 (MQ=255)
                                                                                 tCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCg                                                                                                        >  3:301054/1‑61 (MQ=255)
                                                                                      agcagcTTCAACTTCGTGTTCGACAACCAGATGGAAAAACTGTTGGACGGCGACCGCTTCTACTACCTGGAGCGCACAGCGGGCCTGTCGATGAACGCCGAGGTGGAAAGCAACTCGTTCGCCAAGCTGATCATg                         <  1:292912/135‑1 (MQ=38)
                                                                                      agcaCCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGTAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATg                         <  1:288014/135‑1 (MQ=255)
                                                                                             tCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGCCCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGCGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAg                 >  3:8139/1‑136 (MQ=255)
                                                                                              cAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAg                 >  1:7183/1‑135 (MQ=255)
                                                                                                      tgtTCGAGAACCAGATCGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAGCTCGGccac        <  4:200271/136‑1 (MQ=255)
                                                                                                       gtTCGAGAACCTGTTGCAAAAACTGCAGGACGGCGACCTATTCTACTACCTGGAGCGCACGGCGGGCGTGTGGATTAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAGCTCGGcca         <  6:267268/134‑1 (MQ=37)
                                                                                                       gtTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAGCTCGGccac        <  2:7183/135‑1 (MQ=255)
                                                                                                       gtTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAGCTCGGccac        >  7:352824/1‑135 (MQ=255)
                                                                                                       gtTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCAACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAGCTCGGccac        >  7:314682/1‑135 (MQ=255)
                                                                                                            agaCCCAGATTGAAAAACTGCCGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCAAAGCTGATCCTGGCCAACAGCTCGGCcacccacc   <  5:300054/135‑1 (MQ=255)
                                                                                                             gaACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAGCTCGGCCACCCACCt  <  5:331274/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                   
CAGTCACCGTGACCGGTGTCGATGCCATCGACCTGTGGATCGGCGGCCTGGCGGAAGCAAAAGCGCCATTTGGCGGCATGCTCGGCAGCACCTTCAACTTCGTGTTCGAGAACCAGATGGAAAAACTGCAGGACGGCGACCGCTTCTACTACCTGGAGCGCACGGCGGGCCTGTCGATGAACGCCGAGCTGGAAAGCAACTCGTTCGCCAAGCTGATCATGGCCAACAGCTCGGCCACCCACCT  >  KT2440/2910909‑2911152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑