| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,237,345 | A→C | 35.1% | intergenic (+76/+25) | dinG_3 → / ← CJ019_03036 | 3'‑5' exonuclease DinG/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,237,345 | 0 | A | C | 35.1% | 25.5 / 2.4 | 29 | intergenic (+76/+25) | dinG_3/CJ019_03036 | 3'‑5' exonuclease DinG/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (11/7); new base C (3/8); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGGCGGCTATTGTCTAGCGGTCTTTCGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTCCAGCACAGTGCCGAAACGCTTACCC > KT2440/3237251‑3237473 | ggcggcggcTATTGTCTAGCGGTCTTTCGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAACCCACCCCCCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGt > 3:105454/1‑136 (MQ=255)ggcggcggcTATTGTCTAGCGGTCTTTCGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCACCCCGCCGCAAGGCGGCCCAAGCCCGTCAACCGGGCATTCCg > 1:52875/1‑135 (MQ=255) cTATTGTCTAGCGGTCTTTCGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCAGTCCGTCTGCCCgg > 3:218858/1‑136 (MQ=255) cTATTGTCTAGCGGTCTTTCGCCTTTGACGATCAGTCGCAATCA‑GGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCCCCCCGCCGCAAGGCGGCCCACGCACGTCCACCGGGCATTACGTCTTCCCgg > 2:362868/1‑135 (MQ=255) cTATGGCCTCGCGGGCTTACGCCTTTCCCCATCCGTCGCAACCCGGGGGGGGGAACAAACCACCCACCAAGTCCCCCTCATGCCCCCCCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCgg < 3:64614/136‑1 (MQ=255) cctcgCGGCCTTCCGCCTTTCCCGACCCCGCGCAACCCGGGGGGGCCACCAAACCACCCACCAAGTGCCCCTCATGCCACCCCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCg < 5:132332/131‑1 (MQ=255) tttCGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATgg > 3:314259/1‑136 (MQ=255) tttCGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATg > 1:305044/1‑135 (MQ=255) cGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCCCCCCGCCCCAAGGCGGCCCAATCACGTCAACCGGGCATTCCGTCTGCCCGGCGCCGGAGCAGGAttg > 7:171996/1‑131 (MQ=255) cGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATggg > 3:114804/1‑134 (MQ=255) cGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTACCCTCATGGCACACCGCCGCAAGGCGGCCCCAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGCGCCGGATggt > 4:5378/1‑133 (MQ=255) gCCTTTGACTATAAGTCGCAATCAGGGGGGGGCAACAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGAAAGGCGGCCCAAGCACGTCAACAGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCa < 4:157637/135‑1 (MQ=255) aCGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATg > 6:234181/1‑135 (MQ=255) gggggggAGCAAACCACCCACAAAGTCCCCCTCATGGCACCCCGCCGCACGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAgcg < 4:70717/136‑1 (MQ=255) ggggggcAACAACCCACCCACCAAGCTCCCCTCCGGCCACCCCGCCCCATGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAgcg < 3:208795/136‑1 (MQ=255) gggggtACCAAACCACCCACCAAGTCCCCCTCATGCCACCCCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAgcg < 8:171996/135‑1 (MQ=255) ggggggATCAAACCACCCACAAAGTGCCCCACATGCCACCCCCCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAgcg < 2:8458/135‑1 (MQ=255) ggggggAACAAACCACCAACCAAGGACCCCTCATGGCACCCCGCCGCACGGCGGCCCAAGCACGTCACCCGGGCATTCCGGCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAgcg < 8:277917/135‑1 (MQ=255) gggggAACAAACCCCCCACCAAGTGCCCCTCTTCCACCACCTCCCCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGAGCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGcc < 3:89502/136‑1 (MQ=255) gcgcaccAACCCACCCACCAAGTACCCCTCATGGCACACCGCCCCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGcc < 8:202090/129‑1 (MQ=255) gATCAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGcctgt > 5:332167/1‑132 (MQ=255) caacAAACCCCCCACCACGTACCCCGCCGGCCCCCCCCCCGCACGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAgg < 7:5148/132‑1 (MQ=255) aacccacccacCAAGTACCCCTCATGCCACACCCCCGCACGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTCCa < 4:218858/133‑1 (MQ=255) aaaCCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTc > 3:17185/1‑134 (MQ=255) aaCCACCCACAAAGGTCCCCTCATGCCACACCGCCGCACGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTCCa < 8:360654/135‑1 (MQ=255) accaccCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTCCAg > 6:259129/1‑135 (MQ=255) ccacccacCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGACCAGCAACAGCGCCAGGCTCCAGc > 8:116321/1‑135 (MQ=255) aTGGAACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCAGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTCCAGCACAGTGCCGAAACGCTTAccc < 4:264848/136‑1 (MQ=255) tgccacaCCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTCCAGCACAGTGCCGAAACGCTTAc < 6:135462/130‑1 (MQ=255) tGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTCCAGCACAGTGCCGAAACGCTTAccc < 2:69628/135‑1 (MQ=255) | GGCGGCGGCTATTGTCTAGCGGTCTTTCGCCTTTGACGATCAGTCGCAATCAGGGGGGGGGATCAAACCACCCACCAAGTTCCCCTCATGGCACACCGCCGCAAGGCGGCCCAAGCACGTCAACCGGGCATTCCGTCTGCCCGGGGCCGGAGCAGGATGGGCAGGTAATGGCGCAGGAACAGCAACAGCGCCAGGCTCCAGCACAGTGCCGAAACGCTTACCC > KT2440/3237251‑3237473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |