Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,308,718	A→C	36.3%	R166R (CGT→CGG)	CJ019_03110 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,308,718	0	A	C	36.3%	25.9 / 7.5	39	R166R (CGT→CGG)	CJ019_03110	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (14/10);  new base C (3/12);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 2.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAGGG  >  KT2440/3308589‑3308847                                                                                                                                  |                                                                                                                                  acaGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGcccc                                                                                                                             >  4:57600/1‑136 (MQ=255)      tGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCCCCCCCTGCCCCCGCCAGCCGTTGCGCCGCTCGCCCCGCCGCCACCGCCCCAGGc                                                                                                                         >  1:131431/1‑135 (MQ=255)      tGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACGAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGc                                                                                                                         >  7:131409/1‑135 (MQ=255)      tGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGc                                                                                                                         >  8:370842/1‑135 (MQ=255)      tGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGc                                                                                                                         >  1:303455/1‑135 (MQ=255)           gCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCCGGCCGCgg                                                                                                                    >  7:281870/1‑135 (MQ=255)           gCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCgg                                                                                                                    >  7:80334/1‑135 (MQ=255)               gcgcCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCACAGCAGCCAACGCCCCAGGCCGCGGCCGaa                                                                                                               <  3:232044/136‑1 (MQ=255)                 gcCTGCTGGCCCTGGTGTGGTTCCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCTaaa                                                                                                              <  2:303455/135‑1 (MQ=255)                  ccTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAAc                                                                                                             >  1:209443/1‑135 (MQ=255)                             aGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCCGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGgcg                                                                                                  >  5:208910/1‑135 (MQ=255)                              ggggttgttCCAACCCGGCGCCGCGCGCGGCGCCCAGCAACATGCGGGCCCAAGCCACCAACTGCCCCCGCCAGCCGTTGCGCCGCGCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGgcgc                                                                                                 <  6:147129/132‑1 (MQ=255)                                 gttgttGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCACCGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCg                                                                                              >  2:86780/1‑135 (MQ=255)                                  ttgttcCACCCCGGCGCCGCGCGCGGCGCCCACCAACATGCCGGCCCACGGCACCACCTCCCCCCCCCAGCCGTGGCGCCGCGCGCCCAGCAGCCAACGCCCCCGGCCGCGGCCGAAACGCGACAGGGCGCCCGt                                                                                             <  5:98846/135‑1 (MQ=255)                                          cccAGGCGCCGCGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTCCCCCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCACCGCCCCCGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAg                                                                                     <  7:61268/134‑1 (MQ=255)                                          aCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCGCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAg                                                                                     <  8:276034/135‑1 (MQ=255)                                               gcgcCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGc                                                                               >  4:121473/1‑136 (MQ=255)                                                      gcgcGGCGCCCCGCACCAGGCCGGCCCACGCCACCAACTGCCCCCGCCCGCCGTGGCGCCGCTCGCCCCGCACCCACCGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATgg                                                                         <  7:334731/135‑1 (MQ=255)                                                      gcgcGGCGCCCAGCAACATGCCGGCCCACGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCGCGCCCCGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATgg                                                                         <  2:16814/135‑1 (MQ=255)                                                      gcgcGGCGCCCAGCAACATGCCGGCCCACGCCACCAACTGCACCCGCCAGCCGTGGCGCCGCGCGCCCAGCAGCCACCGCCCCCGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATgg                                                                         <  8:349203/135‑1 (MQ=255)                                                          ggcgCCCAGAAACATGCCGGCCCACGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCGCGCCCAGCAGCCACCGCCCCAGCCGGCGGCCTAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAgg                                                                    <  4:347862/136‑1 (MQ=255)                                                            cgcCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAgg                                                                    >  5:309493/1‑134 (MQ=255)                                                                 cgCAACATGCCGGCCCAAGGCACCAACTGCCCCCGCCACCCGTGGCGCCGCTCGCCCAGCAGCCACCGCCCCAGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTc                                                              <  2:18097/134‑1 (MQ=255)                                                                 accAACCTGCCGCCCCACGGCACCACCGCCCCCCCCCCGCCGTGGCGCCGCGCGCCCCCCAGCCACCGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTc                                                              <  5:247652/133‑1 (MQ=255)                                                                 aGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCGCGCCCAGCAGCCAACGCCCCCGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTc                                                              <  8:173275/135‑1 (MQ=255)                                                                            ggCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCAc                                                   >  1:263885/1‑135 (MQ=255)                                                                               ccacgccACCAACGCCACCCCCCCGCGGTGGCGCCGCGCCCCCCGCACCAAACCCCCCCGGCCGCGGCCTAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGAcc                                               <  3:75529/130‑1 (MQ=255)                                                                                      aCCAACTGCACCCGCCCCCCGTGGCGCCGCGCGCCCCCCACCCACCGCCCCGGGCCGCGCCCTAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGa                                        <  3:252373/136‑1 (MQ=255)                                                                                           cTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCAt                                    >  5:266551/1‑135 (MQ=255)                                                                                            tGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCATCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGAGCCCGTTACGTAAGTCAGGCCGATGGGAAGGCCAATCACATGTTCAACGACCGCAGTGATCATg                                   >  6:309009/1‑135 (MQ=255)                                                                                                              cgccgcTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGgccg                >  4:131970/1‑136 (MQ=255)                                                                                                              cgccgcGCGCCCCGCAGCCAACGCCCCAGGCCGGGGCGGAAACGCGACAGGGCGCCCGTTACGTAAGGCAGGCCGATGGGCAGGCCATTCACCTGTGCCACGACCGCATTGATCATGCCCATGGCCAGCGTGgccg                <  4:54291/136‑1 (MQ=255)                                                                                                               gccgcTCCCCCAGCAGCCACCGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGgccgc               <  3:137085/136‑1 (MQ=255)                                                                                                                ccgcTCGCCCACCTGCCCCCGCCCCCGCCGGGGCCCTAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGgccgc               <  1:86780/135‑1 (MQ=255)                                                                                                                ccgcGCGCCCACCCCCCACCGCCCCAGGCCGCGGCCGAACCGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGgccgc               <  2:214455/135‑1 (MQ=255)                                                                                                                   cgcGCCCAGCAGCCACCGCCCCCGGCCGGGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCaa            <  5:124112/133‑1 (MQ=255)                                                                                                                   cTCGCCCAGCAGCCAACGCCCCCGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCaa            <  7:370842/135‑1 (MQ=255)                                                                                                                      gCCCAGCAGCCACCGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACCgc        >  3:162916/1‑136 (MQ=255)                                                                                                                            ccgcCACCGCCCCCGCCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGAGGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAggg  <  4:249615/134‑1 (MQ=255)                                                                                                                                  |                                                                                                                                  ACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCACCTGTTCCACGACCGCATTGATCATGCCCATGGCCAGCGTGGCCGCCAACAGCGCAGGG  >  KT2440/3308589‑3308847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑