Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,568,762 A→G 6.6% M262T (ATG→ACG)  uspE_2 ← Universal stress protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,568,7620AG 6.6% 193.2 / 4.3 61M262T (ATG→ACG) uspE_2Universal stress protein E
Reads supporting (aligned to +/- strand):  ref base A (35/22);  new base G (2/2);  total (37/24)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

GGCCCCTGGGCAGCCTGCTGAAGCTCAGCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCGCTGCGC  >  KT2440/3568632‑3568895
                                                                                                                                  |                                                                                                                                     
ggCCCCTGGGCAGCCTGCTGAAGCTCAGCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGa                                                                                                                                    >  1:325363/1‑134 (MQ=255)
                gctgAAGCTCAGCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCgg                                                                                                                   >  8:98224/1‑135 (MQ=255)
                gctgAAGCTCAGCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCgg                                                                                                                   >  8:81130/1‑135 (MQ=255)
                gctgAAGCTCAGCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGt                                                                                                                  >  3:202916/1‑136 (MQ=255)
                       cTCAGCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGcc                                                                                                           <  3:262775/136‑1 (MQ=255)
                          aGCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCcgac                                                                                                        >  3:156210/1‑136 (MQ=255)
                           gCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCcgac                                                                                                        <  2:153997/135‑1 (MQ=255)
                              aaCAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGaa                                                                                                     >  6:201693/1‑135 (MQ=255)
                                 aGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTgg                                                                                                 <  4:90424/136‑1 (MQ=255)
                                   ccTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTggg                                                                                                <  1:333004/135‑1 (MQ=255)
                                                cacaTTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGAt                                                                                   >  7:199684/1‑135 (MQ=255)
                                                cacaTTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGAt                                                                                   >  2:115271/1‑135 (MQ=255)
                                                cacaTTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGAt                                                                                   >  7:41446/1‑135 (MQ=255)
                                                cacaTTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGACGAATGTCTGCATGGCGTCATGCAGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGACCTGGGCAATGCCCCTGAt                                                                                   >  2:322382/1‑135 (MQ=255)
                                                 acaTTGCACAACGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATcg                                                                                 <  4:10954/136‑1 (MQ=255)
                                                  caTTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGCGGCCGACGAACTGGGCAATGGCCCTGATcgc                                                                                <  4:217758/136‑1 (MQ=255)
                                                   aTTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATcgc                                                                                <  6:154449/135‑1 (MQ=255)
                                                      gCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGc                                                                             >  5:270411/1‑135 (MQ=255)
                                                       cacaGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGccc                                                                           <  4:125506/136‑1 (MQ=255)
                                                        acaGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTc                                                                                                                          >  8:47125/1‑88 (MQ=255)
                                                        acaGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTc                                                                                                                          <  7:47125/88‑1 (MQ=255)
                                                        acaGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGccc                                                                           >  2:62987/1‑135 (MQ=255)
                                                             gggTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCg                                                                      <  1:62987/135‑1 (MQ=255)
                                                              ggtggtCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGa                                                                     <  6:177942/135‑1 (MQ=255)
                                                              ggTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGa                                                                     >  7:246323/1‑135 (MQ=255)
                                                               gtgtTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCGTGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAg                                                                    >  4:137555/1‑135 (MQ=255)
                                                               gtgtTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCGTGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAg                                                                    >  4:131827/1‑135 (MQ=255)
                                                               gtgtTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTTGGCAATGGCCCTGATCGCGGCCCCTTCGa                                                                     >  1:132463/1‑134 (MQ=255)
                                                               gtgtTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGa                                                                     >  1:7899/1‑134 (MQ=255)
                                                               gtgtTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAgg                                                                   <  3:93263/136‑1 (MQ=255)
                                                               gtgtTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAg                                                                    >  2:68836/1‑135 (MQ=255)
                                                                       gCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCGTGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCg                                                           <  3:137555/136‑1 (MQ=255)
                                                                       gCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCGTGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCg                                                           <  3:131827/136‑1 (MQ=255)
                                                                         acaTGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCGTCGGGGACGTGCCgg                                                          >  5:52642/1‑135 (MQ=255)
                                                                           aTGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGgcgc                                                       >  3:221576/1‑136 (MQ=255)
                                                                           aTGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGgcgc                                                       >  3:225254/1‑136 (MQ=255)
                                                                               gcCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTc                                                     >  4:180319/1‑134 (MQ=255)
                                                                               gcCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCGcc                                                    >  5:274664/1‑135 (MQ=255)
                                                                                ccGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCa                                                   <  2:325363/135‑1 (MQ=255)
                                                                                ccGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAg                                                  >  4:84418/1‑136 (MQ=255)
                                                                                        ccgccgAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCCACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATc                                           >  7:76083/1‑135 (MQ=255)
                                                                                            cgATGAATGTATGCATGGCGTCTTGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGt                                       <  2:132463/135‑1 (MQ=255)
                                                                                              aGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGa                                     >  2:112752/1‑135 (MQ=255)
                                                                                                   tGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGCTCTGGTGATGGTCg                               >  4:324701/1‑136 (MQ=255)
                                                                                                      cTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGccc                            <  4:225254/136‑1 (MQ=255)
                                                                                                       tGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTGGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGccc                            <  5:46497/135‑1 (MQ=255)
                                                                                                          aTGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGccc                            <  1:112752/132‑1 (MQ=255)
                                                                                                          aTGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAgg                         <  7:81130/135‑1 (MQ=255)
                                                                                                              cGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGCTCGCGGCCCCTTCGAGGAAGTGCCTGCGCTCCAGCGGGATGGGGGGGTGGTCGCCCAGGCGGt                     >  1:356017/1‑135 (MQ=255)
                                                                                                                tCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGtt                    >  1:240505/1‑134 (MQ=255)
                                                                                                                 cATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGa                  >  7:334772/1‑135 (MQ=255)
                                                                                                                  aTGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGaa                 >  1:15516/1‑135 (MQ=255)
                                                                                                                      cGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCGGGTGATGGTCGCCCCGGCGGTTGAACGCAg            >  3:22856/1‑136 (MQ=255)
                                                                                                                           gCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCg        >  7:366922/1‑135 (MQ=255)
                                                                                                                           gCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCg        >  5:188356/1‑135 (MQ=255)
                                                                                                                           gCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCg        >  2:222354/1‑135 (MQ=255)
                                                                                                                           gCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCGc       >  4:185328/1‑136 (MQ=255)
                                                                                                                           gCTGCCCATGACGATCACGTCCACCCGGTTGCGGCCGACGAACTGGGCAATGGCCCGGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCGc       <  3:204472/136‑1 (MQ=255)
                                                                                                                                ccATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCGCTgcgc  <  4:156210/136‑1 (MQ=255)
                                                                                                                                 cATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCGCTgcgc  <  8:241875/135‑1 (MQ=255)
                                                                                                                                 cATGACGATCACGTCCACCCGGTTGGGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCGCTgcgc  <  1:57964/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
GGCCCCTGGGCAGCCTGCTGAAGCTCAGCGAACAGCCTTGATCGCCAACACATTGCACAGCGGGTGTTCCAGCACATGCGCCGTTGTCCCGCCGAGGAATGTCTGCATGGCGTCATGCCGGTGGCTGCCCATGACGATCACGTCCACCCGGTTGTGGCCGACGAACTGGGCAATGGCCCTGATCGCGGCCCCTTCGAGGAAGTGCCGGCGCTCCAGCGGGATCTGGTGATGGTCGCCCAGGCGGTTGAACGCAGCCCGCTGCGC  >  KT2440/3568632‑3568895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑