Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,599,230 A→G 6.3% L197L (TTG→CTG)  xerC_4 ← Tyrosine recombinase XerC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,599,2300AG 6.3% 199.4 / 3.5 63L197L (TTG→CTG) xerC_4Tyrosine recombinase XerC
Reads supporting (aligned to +/- strand):  ref base A (27/32);  new base G (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

GCCCACCAGGCTGTTGCTGTTGAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGATGTGTTCGATGCGCAGGCGTGCCAGCTCAT  >  KT2440/3599102‑3599361
                                                                                                                                |                                                                                                                                   
gCCCACCAGGCTGTTGCTGTTGAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCt                                                                                                                                >  2:40350/1‑134 (MQ=255)
    agcaGGCTGTTGCTGTTGAGCGCGTTGTGGCCAAGGGTGCCCCAGCGGTCGAGTTTGCGGAACACCTGCCCATGGGCAATACCGGAGACTTCAAGCAATTGCAGGTAGGCAGTGACAGTGCACAAGGCCTTGAtgg                                                                                                                          <  4:325972/134‑1 (MQ=255)
        ggCTGTTGCTGTTGAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAgg                                                                                                                      >  4:311392/1‑136 (MQ=255)
          ctgttgctgttgAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAggg                                                                                                                     <  1:44094/135‑1 (MQ=255)
          ctgttgctgttgAGCGCGTTGGCGCCAAGGGTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAggg                                                                                                                     <  5:72959/135‑1 (MQ=255)
          ctggtgctgttgAGCGCTGTGTGGCAAAGTTTGGCCCCGCGGTCGAGTTTGCGGAACACCTGCCCATTGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGCGACTCGGCACAAGGCCTTGAGGGCAggg                                                                                                                     <  6:247379/135‑1 (MQ=255)
                ctgttgAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAGGGCCTTGAGGGCAGGggtgcggt                                                                                                              >  3:254014/1‑136 (MQ=255)
                ctgttgAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTATGCGGAACACCGGCCCATGGCCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAGCGCCTTGAGGGCAGGggtgcggt                                                                                                              >  3:249426/1‑136 (MQ=255)
                 tgttgAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGggtgcggt                                                                                                              >  7:85664/1‑135 (MQ=255)
                   ttgAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGggtgcggtgc                                                                                                            <  3:310808/135‑1 (MQ=255)
                   ttgAGCGCGTTGTCGCCAAGGGTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGggtgcggtgc                                                                                                            <  4:302045/135‑1 (MQ=255)
                    tgAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGggtgcggtgc                                                                                                            <  1:28634/134‑1 (MQ=255)
                     gAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTgg                                                                                                         >  3:265904/1‑136 (MQ=255)
                      aGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCCGCCCATCGGCAATACCCGCGCCTTCAAACCATTGCCTGTACGCAGTGACAGGGCACAAGGCCTAGATGGCAGGGGTGCGGTGCTgg                                                                                                         >  5:199236/1‑135 (MQ=255)
                       gcgcGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGAc                                                                                                       <  4:145589/136‑1 (MQ=255)
                          cgTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGc                                                                                                     <  8:311935/135‑1 (MQ=255)
                                cGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAgg                                                                                               >  2:94730/1‑135 (MQ=255)
                                cGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGc                                                                                              >  4:73518/1‑136 (MQ=255)
                                    aaGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTc                                                                                           <  2:22326/135‑1 (MQ=255)
                                     agggggCCCCAGCGGTCGAGTGTGCGGAACACCGGCCCATGGGCAATACCGGCGACTGCAAGCCATGGCAGGGAGGCAGGGACCGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTcgc                                                                                         <  4:238523/131‑1 (MQ=255)
                                     aGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTGGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTcgc                                                                                         >  3:347951/1‑136 (MQ=255)
                                      gggtGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTcgc                                                                                         <  6:151900/132‑1 (MQ=255)
                                      ggTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTcgc                                                                                         <  5:263290/135‑1 (MQ=255)
                                      ggTGGCCCCAGCGGGCGAGTTTGCGGAACCCCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTcgc                                                                                         <  5:236136/135‑1 (MQ=255)
                                        ttGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCgg                                                                                       <  8:192082/135‑1 (MQ=255)
                                          gCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCg                                                                                    >  4:88067/1‑136 (MQ=255)
                                            cccAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGc                                                                                   >  7:89668/1‑135 (MQ=255)
                                                    tCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTtgctg                                                                           >  5:224717/1‑135 (MQ=255)
                                                    gcGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCAACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTtgctg                                                                           <  2:147656/134‑1 (MQ=255)
                                                            gCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTACGCAGTGACAGGGCACAAGGCGTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCGTGCTGCGCGGCAg                                                                   <  1:274620/135‑1 (MQ=255)
                                                             cGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAg                                                                   <  7:239646/134‑1 (MQ=255)
                                                             cGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAg                                                                   <  1:94730/134‑1 (MQ=255)
                                                             cGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAg                                                                   <  1:217300/134‑1 (MQ=255)
                                                                 acacCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAg                                                             >  3:134787/1‑136 (MQ=255)
                                                                               gCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGaca                                                >  1:23797/1‑135 (MQ=255)
                                                                                cAATACCGGCGACTTCAAGCCATTTCAGGTAGGCAGTGACAGGGCACAATGCCTTGAGGGCAGGGGTGCGGTGCTGGACGACCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAACGTAAAGCCGACAcc                                              >  4:207434/1‑136 (MQ=255)
                                                                                cAATACCGGCGACTTCAAGCCATTGCATGTAGGCAGTGACAGGTCACTAGGCCTTGAGGTCAGGGGTGCGGTGCTGGACGCCCTAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGAGAcc                                              <  4:231858/136‑1 (MQ=255)
                                                                                cAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGacac                                               >  6:363241/1‑135 (MQ=255)
                                                                                cAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGacac                                               >  6:140144/1‑135 (MQ=255)
                                                                                cAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACAcc                                              <  4:173264/136‑1 (MQ=255)
                                                                                 aaTACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACAcc                                              <  5:307398/135‑1 (MQ=255)
                                                                                 aaTACCGGCGACTTCAAGCCAGTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACAcc                                              <  2:141698/135‑1 (MQ=255)
                                                                                 aaTACCGGCGACTTCAAGCCAATGCAGCTACGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAACGCGTAATGCCCACACCg                                             >  4:150494/1‑136 (MQ=255)
                                                                                  aTACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAGGTCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCgg                                            <  4:254014/136‑1 (MQ=255)
                                                                                  aTACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAGGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCgg                                            <  4:249426/136‑1 (MQ=255)
                                                                                  aTACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCgg                                            <  3:89299/136‑1 (MQ=255)
                                                                                   tACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCgg                                            <  2:60394/135‑1 (MQ=255)
                                                                                           aCTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCACGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGa                                                  >  3:239291/1‑121 (MQ=255)
                                                                                           aCTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGa                                                  <  4:239291/121‑1 (MQ=255)
                                                                                           aCTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCt                                   >  4:13535/1‑136 (MQ=255)
                                                                                           aCTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACAGGCTTGCGcc                                    >  1:335064/1‑135 (MQ=255)
                                                                                            cTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCt                                   >  2:140055/1‑135 (MQ=255)
                                                                                                 aGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGAtgt                             <  3:48981/136‑1 (MQ=255)
                                                                                                  gCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGAtgt                             <  2:23797/135‑1 (MQ=255)
                                                                                                          aGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTTATGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTTCATGTGTTAGATg                     >  5:73240/1‑135 (MQ=255)
                                                                                                          aGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCTGGAAAAGCGTAAAGCCGCCACCGGATTGCGCCTGGATGTGTTCGCTgc                    >  4:16943/1‑136 (MQ=255)
                                                                                                           ggTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGATGTGTTCGATgc                    <  6:224717/135‑1 (MQ=255)
                                                                                                                 cAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGATGTGTTCGATGCGCAGGCg             <  3:138390/136‑1 (MQ=255)
                                                                                                                    tGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGATGTGTTCGATGCGCAGGCGTg           >  5:195323/1‑135 (MQ=255)
                                                                                                                       cAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGAtgt                             <  7:173721/114‑1 (MQ=255)
                                                                                                                       cAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGAtgt                             >  8:173721/1‑114 (MQ=255)
                                                                                                                         gggCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGATGTGTTCGATGCGCAGGCGTGCCAGc      >  7:263927/1‑135 (MQ=255)
                                                                                                                             acaAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGATGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGATGTGTTCGATGCGCAGGCGTGCCCGCTCAt  >  5:283312/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                   
GCCCACCAGGCTGTTGCTGTTGAGCGCGTTGTCGCCAAGGTTGCCCCAGCGGTCGAGTTTGCGGAACACCGGCCCATGGGCAATACCGGCGACTTCAAGCCATTGCAGGTAGGCAGTGACAGGGCACAAGGCCTTGAGGGCAGGGGTGCGGTGCTGGACGCCCAAGGCTTCGCGGTCGCCCTTGCTGCGCGGCAGGAAAAGCGTAATGCCGACACCGGCTTGCGCCTGGATGTGTTCGATGCGCAGGCGTGCCAGCTCAT  >  KT2440/3599102‑3599361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑