Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,722,166	G→A	5.8%	V13I (GTA→ATA)	CJ019_03540 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,722,166	0	G	A	5.8%	229.3 / 3.1	69	V13I (GTA→ATA)	CJ019_03540	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (40/25);  new base A (2/2);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

CGTGCAGCATCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCGGGGAAGGGCGAGGCGAAAAAGTG  >  KT2440/3722037‑3722297                                                                                                                                  |                                                                                                                                    cGTGCAGCATCCTGACGCTCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTg                                                                                                                                >  5:317075/1‑135 (MQ=255)   tGCAGCATCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATAT‑GCTGCCTTCACGCCGTGGTCGGTATTGGCa                                                                                                                             <  7:126466/135‑1 (MQ=255)    gcagcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGCCTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAg                                                                                                                            >  4:19427/1‑136 (MQ=255)    gcagcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAg                                                                                                                            >  4:295565/1‑136 (MQ=255)     cagcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAg                                                                                                                            <  2:141274/135‑1 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCTACACTGTAGCGCTGAGATGTTTGTTGATATGGCTGGCTTCACGCCGTGGTCGGTATTGGCAgg                                                                                                                           >  7:127349/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGAGAATGGAGCGCTGAGAGGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCCgg                                                                                                                           >  1:178150/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTTTTGGCAgg                                                                                                                           >  1:184591/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTAtt                                                                                                                                 <  1:369898/129‑1 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTAtt                                                                                                                                 >  2:369898/1‑129 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAgg                                                                                                                           >  1:280583/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAgg                                                                                                                           >  2:120371/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAgg                                                                                                                           >  8:36320/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAgg                                                                                                                           >  6:129425/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAgg                                                                                                                           >  8:68719/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAgg                                                                                                                           >  7:183946/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGc                                                                                                                          >  3:292921/1‑136 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGc                                                                                                                          >  3:244657/1‑136 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGc                                                                                                                          >  3:228614/1‑136 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGATATTGGCAGGc                                                                                                                          >  3:202461/1‑136 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGGCGGTATTGGCAgg                                                                                                                           >  7:351726/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGAGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGc                                                                                                                          >  4:128993/1‑136 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTATTTTGTCTCTGATTCCACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAgg                                                                                                                           >  6:61259/1‑135 (MQ=255)      agcaTCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCAGTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGATATTGGCAGGc                                                                                                                          >  3:198143/1‑136 (MQ=255)          tCCTGACGATCATGTCTGCTCCCGGCTGCTGACTTGTTTAAGATGTGATGTTTTTAGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCTCGCCGTGGTCGGTATTGGCAGGCggg                                                                                                                       <  7:199065/135‑1 (MQ=255)          tCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCggg                                                                                                                       <  8:111238/135‑1 (MQ=255)             tGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGTCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCa                                                                                                                    <  5:204718/135‑1 (MQ=255)                cGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTg                                                                                                                 >  8:203399/1‑135 (MQ=255)                cGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGa                                                                                                                >  3:283963/1‑136 (MQ=255)                cGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTGTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCAGTg                                                                                                                 >  1:319987/1‑135 (MQ=255)                  aTCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTGGATATGGCTGCCTTCACGCCGTGGTCGGTATTGTCAGGCGGGGCATTGATc                                                                                                              >  3:331616/1‑136 (MQ=255)                     cggTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGCTTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCgg                                                                                                            <  6:221599/134‑1 (MQ=255)                     aGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCCCGCCGTGGTAGGTATTTGCAGGCGGGGCATTGCTCgg                                                                                                            >  7:148722/1‑135 (MQ=255)                      ggTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCgg                                                                                                            <  7:36320/134‑1 (MQ=255)                       gTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGtt                                                                                                          >  2:158981/1‑135 (MQ=255)                            cgcccgccAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCATGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGcc                                                                                                     <  3:270993/135‑1 (MQ=255)                              cccgccAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCt                                                                                                  <  4:292921/136‑1 (MQ=255)                               ccgccAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCCGGGCATTGATCGGTTTGGCCGCt                                                                                                  >  2:322762/1‑135 (MQ=255)                               ccgccAGCTAACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTg                                                                                                 >  3:263797/1‑136 (MQ=255)                                    aGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCt                                                                                             >  8:129536/1‑135 (MQ=255)                                    aGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCt                                                                                             >  6:103842/1‑135 (MQ=255)                                    aGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTg                                                                                            >  3:237026/1‑136 (MQ=255)                                          cTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGATATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGcc                                                                                      <  4:202461/136‑1 (MQ=255)                                          cTTCAATAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGATATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGcc                                                                                      <  4:198143/136‑1 (MQ=255)                                                   gATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCgca                                                                              >  1:150177/1‑133 (MQ=255)                                                      ggCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACgg                                                                           >  8:254858/1‑135 (MQ=255)                                                           cTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCa                                                                      >  1:264433/1‑135 (MQ=255)                                                           cTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGGCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCAGCTGGGCTGTTCGCCGTTGCGAACGGGCGCa                                                                      >  8:313184/1‑135 (MQ=255)                                                                   tctcTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCa                                                             <  4:263797/136‑1 (MQ=255)                                                                    ctctGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCa                                                             <  7:203399/135‑1 (MQ=255)                                                                    ctctGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCa                                                             <  8:117276/135‑1 (MQ=255)                                                                    ctctGATTCGACAATGGAGCGCTGAGATGTTTGTTGAGATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCa                                                             <  5:44060/135‑1 (MQ=255)                                                                         aTTCGACCATGGAGCGCTGAGATGTTTGTTGATATTGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCTGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGc                                                        <  6:49005/135‑1 (MQ=255)                                                                             gACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGttt                                                   <  4:341286/136‑1 (MQ=255)                                                                              aCAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGttt                                                   <  8:48008/135‑1 (MQ=255)                                                                              aCAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGGGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGttt                                                   <  8:73534/135‑1 (MQ=255)                                                                                        gcTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCg                                         <  7:151610/135‑1 (MQ=255)                                                                                          tGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGttt                                                   >  2:282304/1‑123 (MQ=255)                                                                                          tGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGttt                                                   <  1:282304/123‑1 (MQ=255)                                                                                            agaTGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGAtgct                                    <  3:34601/136‑1 (MQ=255)                                                                                             gaTGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGAtgct                                    <  1:23824/135‑1 (MQ=255)                                                                                                  ttgttgATATGGCTGCCTGCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCaaa                               <  8:193286/135‑1 (MQ=255)                                                                                                        atatGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCg                         >  7:258334/1‑135 (MQ=255)                                                                                                         tatGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCgg                        <  2:367191/135‑1 (MQ=255)                                                                                                                 ccTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCGGGGAAGggc                <  2:184591/135‑1 (MQ=255)                                                                                                                    tCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCGGGGAAGggcgagg            >  4:60275/1‑136 (MQ=255)                                                                                                                    tCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCGGGGAAGggcgag             >  5:194575/1‑135 (MQ=255)                                                                                                                         ccGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCGGGGAAGGGCGAGGCGaa        >  3:311153/1‑135 (MQ=255)                                                                                                                               tCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCGGGGAAGGGCGAGGCGAAAAAgtg  >  7:45845/1‑135 (MQ=255)                                                                                                                                  |                                                                                                                                    CGTGCAGCATCCTGACGATCAGGTCTGCGCCCGCCAGCTGACTTCATTAAGATGGCATCTTTTTTGTCTCTGATTCGACAATGGAGCGCTGAGATGTTTGTTGATATGGCTGCCTTCACGCCGTGGTCGGTATTGGCAGGCGGGGCATTGATCGGTTTGGCCGCTGGGCTGTTCGCCGTTGCGAACGGGCGCATCGCGGGCATCAGCGGTTTGCTCGGTTCGATGCTGCAAAGTGGCGGGGAAGGGCGAGGCGAAAAAGTG  >  KT2440/3722037‑3722297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑