Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,763,787	A→C	28.2%	E44A (GAG→GCG)	CJ019_03576 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,763,787	0	A	C	28.2%	72.5 / 6.1	64	E44A (GAG→GCG)	CJ019_03576	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (38/6);  new base C (10/10);  total (48/16)
Fisher's exact test for biased strand distribution p-value = 3.93e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATGAGCGTGAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCC  >  KT2440/3763654‑3763920                                                                                                                                      |                                                                                                                                      cTGATGAGCGTGAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAgc                                                                                                                                     <  3:113256/136‑1 (MQ=255)   gatgaGCGTGAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCGATGGTTGGTCTCCGGGGTTGTCATGCTGTTCGTCGGCTACCCCAAGCTGACCCCGGCCGAgcg                                                                                                                                    >  5:104242/1‑135 (MQ=255)     tgaGCGTGAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGcc                                                                                                                                  <  5:254804/135‑1 (MQ=255)            gAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGGGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGCCACCGGCCGCGCGCCTGGcccc                                                                                                                           >  2:69849/1‑134 (MQ=255)            gAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCa                                                                                                                           >  1:239552/1‑135 (MQ=255)            gAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCAc                                                                                                                          >  3:359500/1‑136 (MQ=255)            gAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCGGGTCATGGCGCTAGGGTTCGTCTCCGGGGGTGTCATGCTGTACGTCGGCTACCCCACGCTGCCACCGGCCGCGCGCCTGGcccc                                                                                                                           >  7:174634/1‑134 (MQ=255)              aGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCAcc                                                                                                                         >  8:101488/1‑135 (MQ=255)                     gCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGc                                                                                                                  >  8:204048/1‑135 (MQ=255)                      cTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACCCCGGCCGCGCGCCTGGCCCACCTGCCGGCt                                                                                                                 >  5:69873/1‑135 (MQ=255)                      cTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCCCCTGCCGGCt                                                                                                                 >  8:229953/1‑135 (MQ=255)                           ccTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCcg                                                                                                           <  4:266099/136‑1 (MQ=255)                            cTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTGATGGCGCTATGGGTGGTCTCCGGGGGTGTCATGCTGTACGTCGGCGACCCCAAGCTGACACCGGCCGCGCGCCTGGCCCACCTGCCGGCGGTGCcg                                                                                                           >  2:175338/1‑135 (MQ=255)                                 gCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCCACCTGACACCGGCCGAGCGCCTGGCCCACCAGCCGGCGTTGCCGGCggg                                                                                                      >  5:20514/1‑135 (MQ=255)                                 gCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCggg                                                                                                      >  6:327233/1‑135 (MQ=255)                                  cACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTGATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCgggg                                                                                                     >  1:258918/1‑135 (MQ=255)                                  cACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGCGCGCCTGGCCCACCTGCCGGCTTTGCCGGCgggg                                                                                                     >  8:191661/1‑135 (MQ=255)                                   aCCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCGTTGCCGGCGGGGt                                                                                                    >  8:183302/1‑135 (MQ=255)                                    ccGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGCGCCCCTGGCCCCCCTGCCGGCGTTGACGGCgggggg                                                                                                   >  5:56445/1‑133 (MQ=255)                                        tggctggGCATCGTCGCCTGCCTGTTCATGGCGGTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTGTGCCGGCGGGGGGCTgcg                                                                                              >  3:91555/1‑136 (MQ=255)                                        tggctggGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCCCCTGCCGGCTTTGCCGGCGGGGtgctgc                                                                                               >  2:227346/1‑135 (MQ=255)                                        tggctggGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGtgctgc                                                                                               >  1:10732/1‑135 (MQ=255)                                            tggGCAGCGGCGCCGGCCGGTTCATGGCGCTAGGGGGCGTCGCCGGGGTGGGCAGGCTGTACGGCGGCTACCCCACGCGGACACCGGCCGCGCGCCGGGCCCACCGGCCGGCTTTGCCGGCGGGGTGCTGCGCCg                                                                                           <  1:131001/135‑1 (MQ=255)                                                  tcgtcgCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCCAGCTGACACCGGCCGCGCGCCTGGCCCCCCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGcc                                                                                    >  3:105022/1‑136 (MQ=255)                                                  tcgtcgCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGCGCGCCTGGCCCACCTGCCGGCGTTGCCGGCGGGGTGCTGCGCCGAGTTGc                                                                                     >  8:114757/1‑135 (MQ=255)                                                  tcgtcgCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGc                                                                                     >  8:109751/1‑135 (MQ=255)                                                       gcctgcctGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAg                                                                                >  3:302442/1‑135 (MQ=255)                                                       gcctgcctGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGc                                                                               >  3:117023/1‑136 (MQ=255)                                                            cctgCTCAGGGCGCTATGGTTCGTCGCCGGGGTGGCCATGCTGTACGCCGGCCACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTGGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCAtt                                                                            <  3:197038/134‑1 (MQ=255)                                                              tttgcATGGCGCTATGGTGCGTCGCCGGGGTGGTCAGGCTGTCCGTCGGCACCCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTGTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTggg                                                                         <  6:272364/131‑1 (MQ=255)                                                               gTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCCCCTGCCGGCTTTGCCGGCGGGGTGCTTCGCCGAGTTGCACGAGCATTGGgcg                                                                       >  4:32339/1‑136 (MQ=255)                                                                ttCATGGCGCTATGGTTCGGCGCGGGGGTTGGCATGCTGTACGTCGGCTCCCCCAAGCTGACACCGGCCGCGCGCCTGGCCCACCTGCCGGTTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTggg                                                                         <  1:270698/133‑1 (MQ=255)                                                                   aTGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAATCTGACACCGGCCCAGCGCCTGGCCCCCCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGCGCATTTGGCGCa                                                                     >  7:149032/1‑134 (MQ=255)                                                                        gcTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGCGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGcc                                                               >  1:7019/1‑135 (MQ=255)                                                                        gcTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGCGCATTGGGCGCAACAGcc                                                               >  2:184055/1‑135 (MQ=255)                                                                          tagggTTCGCCGCCGGGGTTGGCATGCGGTACGTCGGCTACCCCAAGCTGACACCGGCCGCGCGCCGGGCCCACCGGCCGGCTTGGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCt                                                            <  4:359500/133‑1 (MQ=255)                                                                             ggTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTCACACCGGCCGAGCGCCTGGCCCACCAGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCt                                                            >  2:215537/1‑133 (MQ=255)                                                                                gcGTCGCCGGGGTTGTCATGCTGTCCGGCGGCTACCCCAAGCTGACCCCGGCCGCGCGCCTGGCCCCCCGGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAgg                                                       <  5:327233/134‑1 (MQ=255)                                                                                  gTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGgcgc                                                    >  4:278474/1‑136 (MQ=255)                                                                                    ctcCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTTCCGGCGGGGTGCTGCACCGAGTTGCCCGAGCATTGGGCGCACCAGCCGCTGAAGGCGCt                                                   >  2:276743/1‑135 (MQ=255)                                                                                    ctcCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCt                                                   >  7:297681/1‑135 (MQ=255)                                                                                       cGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTgcgc                                               >  4:57540/1‑136 (MQ=255)                                                                                         gggTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGACGGCGCTGCGcc                                              >  7:309672/1‑135 (MQ=255)                                                                                          ggtggtCCTGCGGTCCGCCGGCTACCCCACGCTCACCCGGGCCGCGCGCCTGGCCCCCCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCt                                             <  2:285946/135‑1 (MQ=255)                                                                                          ggTTGTCATGCTGTAGGTCGGCTACCCCACGCTGACACCGGCCGCGCGCGTGCCCCCCGTCCCGGCTTGGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCt                                             <  6:20514/135‑1 (MQ=255)                                                                                          ggTTGTCATGCTGTACGGCGGCTACCCCAAGCGGACACCGGCCGAGCGCCGGGCCCACCGGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCt                                             <  8:276089/135‑1 (MQ=255)                                                                                                  tGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGCAGGCGCTTCGCCTGACCCGcc                                     >  6:213537/1‑135 (MQ=255)                                                                                                        ccggcGGCTACCCAACGCGCACCCCGCCCGCGCGCCTGCCCCCCCTGCGGGCTTGGCGGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcgg                              <  3:146790/132‑1 (MQ=255)                                                                                                          gTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcggg                             >  2:314289/1‑135 (MQ=255)                                                                                                            cGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTTCGCCTGACCAGCCTGGGCGGgcgc                          >  3:219937/1‑136 (MQ=255)                                                                                                              gCTCCCCCACGCTGACACGGGCCGCGCGCCGGGCCCCCCTGCCGGCTTTGCGGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGgcgcgc                        <  3:122850/136‑1 (MQ=255)                                                                                                               cTACCCCAAGCTGCCACCGGCCGCGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCAGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGgcgcgc                        >  6:75455/1‑135 (MQ=255)                                                                                                                  ccccACGCTGACACCGGCCGCGCGCCTGGCCCACCTGCCGGCTTGGCGGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGcac                    <  3:284808/136‑1 (MQ=255)                                                                                                                  ccccAAGCTCACACCGGCCGCGCGCCTGGCCCCCCTGCCGGCTTGGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGcac                    <  3:319066/136‑1 (MQ=255)                                                                                                                    ccAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTAACCAGCCTGGGCGGGCGCGCGcacc                   >  1:215403/1‑135 (MQ=255)                                                                                                                      aaGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCACTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCAGACCCGCCTGGGCGGGCGCGCGcacccc                 >  1:353221/1‑133 (MQ=255)                                                                                                                          tGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCCCctgct            >  4:72441/1‑136 (MQ=255)                                                                                                                          tGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACctgc             >  5:171160/1‑135 (MQ=255)                                                                                                                               ccGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAAc       >  3:158359/1‑136 (MQ=255)                                                                                                                                cGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACt      >  4:156782/1‑136 (MQ=255)                                                                                                                                     gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGGGCATTTGGCGCAACAGCCGCTGCAGGCCCTGCGCCGGACCAGCCTGGGCGGGCCCGCGCCCCCCCTGGGGGGACAGGcc  >  2:183822/1‑135 (MQ=255)                                                                                                                                     gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGCGCATTGGGCGCAACAGCCGCTGACGGCGCTGCGCCTGCCCAGCCTGGGCGGGCGCGCGCACCCCCTGCTGGCACTGGcc  >  8:145944/1‑135 (MQ=255)                                                                                                                                     gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGCAGGCGCTGCGCCTGACCCGCCTGGGCGGGCGCGCGCCCCACCTGCTGGAACTGGcc  >  6:234536/1‑135 (MQ=255)                                                                                                                                     gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGcc  >  7:249629/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                      CTGATGAGCGTGAAGCGCCAGCTGTACCTGTGGCACCGCTGGCTGGGCATCGTCGCCTGCCTGTTCATGGCGCTATGGTTCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCC  >  KT2440/3763654‑3763920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑