Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,763,865	A→C	18.6%	K70T (AAG→ACG)	CJ019_03576 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,763,865	0	A	C	18.6%	117.5 / 3.5	59	K70T (AAG→ACG)	CJ019_03576	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/20);  new base C (2/11);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGG  >  KT2440/3763733‑3763993                                                                                                                                     |                                                                                                                                 gcGTCGCCGGGGTTGTCATGCTGTCCGGCGGCTACCCCAAGCTGACCCCGGCCGCGCGCCTGGCCCCCCGGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAgg                                                                                                                                <  5:327233/134‑1 (MQ=255)   gTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGgcgc                                                                                                                             >  4:278474/1‑136 (MQ=255)     ctcCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTTCCGGCGGGGTGCTGCACCGAGTTGCCCGAGCATTGGGCGCACCAGCCGCTGAAGGCGCt                                                                                                                            >  2:276743/1‑135 (MQ=255)     ctcCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCt                                                                                                                            >  7:297681/1‑135 (MQ=255)        cGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTgcgc                                                                                                                        >  4:57540/1‑136 (MQ=255)          gggTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGACGGCGCTGCGcc                                                                                                                       >  7:309672/1‑135 (MQ=255)           ggtggtCCTGCGGTCCGCCGGCTACCCCACGCTCACCCGGGCCGCGCGCCTGGCCCCCCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCt                                                                                                                      <  2:285946/135‑1 (MQ=255)           ggTTGTCATGCTGTAGGTCGGCTACCCCACGCTGACACCGGCCGCGCGCGTGCCCCCCGTCCCGGCTTGGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCt                                                                                                                      <  6:20514/135‑1 (MQ=255)           ggTTGTCATGCTGTACGGCGGCTACCCCAAGCGGACACCGGCCGAGCGCCGGGCCCACCGGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCt                                                                                                                      <  8:276089/135‑1 (MQ=255)                   tGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGCAGGCGCTTCGCCTGACCCGcc                                                                                                              >  6:213537/1‑135 (MQ=255)                         ccggcGGCTACCCAACGCGCACCCCGCCCGCGCGCCTGCCCCCCCTGCGGGCTTGGCGGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcgg                                                                                                       <  3:146790/132‑1 (MQ=255)                           gTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTgggcggg                                                                                                      >  2:314289/1‑135 (MQ=255)                             cGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTTCGCCTGACCAGCCTGGGCGGgcgc                                                                                                   >  3:219937/1‑136 (MQ=255)                               gCTCCCCCACGCTGACACGGGCCGCGCGCCGGGCCCCCCTGCCGGCTTTGCGGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGgcgcgc                                                                                                 <  3:122850/136‑1 (MQ=255)                                cTACCCCAAGCTGCCACCGGCCGCGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCAGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGgcgcgc                                                                                                 >  6:75455/1‑135 (MQ=255)                                   ccccACGCTGACACCGGCCGCGCGCCTGGCCCACCTGCCGGCTTGGCGGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGcac                                                                                             <  3:284808/136‑1 (MQ=255)                                   ccccAAGCTCACACCGGCCGCGCGCCTGGCCCCCCTGCCGGCTTGGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGcac                                                                                             <  3:319066/136‑1 (MQ=255)                                     ccAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTAACCAGCCTGGGCGGGCGCGCGcacc                                                                                            >  1:215403/1‑135 (MQ=255)                                       aaGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCACTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCAGACCCGCCTGGGCGGGCGCGCGcacccc                                                                                          >  1:353221/1‑133 (MQ=255)                                           tGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCCCctgct                                                                                     >  4:72441/1‑136 (MQ=255)                                           tGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACctgc                                                                                      >  5:171160/1‑135 (MQ=255)                                                ccGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAAc                                                                                >  3:158359/1‑136 (MQ=255)                                                 cGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACt                                                                               >  4:156782/1‑136 (MQ=255)                                                      gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGGGCATTTGGCGCAACAGCCGCTGCAGGCCCTGCGCCGGACCAGCCTGGGCGGGCCCGCGCCCCCCCTGGGGGGACAGGcc                                                                           >  2:183822/1‑135 (MQ=255)                                                      gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGCGCATTGGGCGCAACAGCCGCTGACGGCGCTGCGCCTGCCCAGCCTGGGCGGGCGCGCGCACCCCCTGCTGGCACTGGcc                                                                           >  8:145944/1‑135 (MQ=255)                                                      gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGCAGGCGCTGCGCCTGACCCGCCTGGGCGGGCGCGCGCCCCACCTGCTGGAACTGGcc                                                                           >  6:234536/1‑135 (MQ=255)                                                      gAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGcc                                                                           >  7:249629/1‑135 (MQ=255)                                                             tGGCCCCCCTGCCGGCTTTGCGGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTc                                                                    <  7:241829/135‑1 (MQ=255)                                                                       gCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAgg                                                                                                                                <  7:113288/65‑1 (MQ=255)                                                                       gCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAgg                                                                                                                                >  8:113288/1‑65 (MQ=255)                                                                          ggatttGCGGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTc                                                      <  3:144480/133‑1 (MQ=255)                                                                                  cggcggGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCCCCTGCTGGAACTGGCCGCCGGTCGCCGCGTCATCCGCGATgccg                                               >  2:78386/1‑135 (MQ=255)                                                                                  cggcggGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGACCTGGCCGACGGTCGCCGCGTCATCCTCGATgccg                                               >  5:280928/1‑135 (MQ=255)                                                                                        ggggctgcGCCGAGGTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCGGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAgcg                                         <  8:256779/132‑1 (MQ=255)                                                                                        ggggcggcgcCGCGTGGCCCGCGCATGGGGCGCACCAGCCGCTGACGGGGCGGCGCCTGCCCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAgcg                                         <  1:138572/129‑1 (MQ=255)                                                                                        ggTGCTGCGCCGAGTGGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCGGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAgcg                                         <  7:229953/135‑1 (MQ=255)                                                                                             tgcGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCCCCCCCGGCTGGCACTGGCCGACGGTCGCCGCGGCCTCCGCGCTGCCGCCCgcggcgcg                                    >  6:229741/1‑133 (MQ=255)                                                                                              gcgcCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCTTgggcgggc                                                                                                     <  8:309311/69‑1 (MQ=255)                                                                                              gcgcCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCTTgggcgggc                                                                                                     >  7:309311/1‑69 (MQ=255)                                                                                                      gtGCCCGAGCATTGGGCGCAACAGCCGCTGACGGCGCTGCGCCGGACCCGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCAGCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTc                          <  4:91555/135‑1 (MQ=255)                                                                                                       tGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATGCTCGATGCCGCCAGCGGCGCGCCTCTGGCTc                          >  6:289122/1‑135 (MQ=255)                                                                                                       tGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTc                          >  2:307877/1‑135 (MQ=255)                                                                                                           cGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGGCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGc                      <  8:309672/135‑1 (MQ=255)                                                                                                             cgCAGTGGGCGCAACCGCGGCGGACGGCGCTGCGCCTGACCCGCGTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCg                    <  8:174634/134‑1 (MQ=255)                                                                                                             aGCATGGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCACCCGGGGCGGGCGCGCGCACCACCGGCTGGACCTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCg                    <  1:242943/135‑1 (MQ=255)                                                                                                              ggaTTGGGCGCAACAGCCGCTGAAGGCGCGGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGa                   <  6:69873/133‑1 (MQ=255)                                                                                                                 ttGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCTCCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGCTGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGc                >  6:333627/1‑135 (MQ=255)                                                                                                                   gggCGCAACAGCCGCTGACGGCGCGGCGCCTGACCCGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAg              <  3:70882/135‑1 (MQ=255)                                                                                                                    ggCGCAACAGCCGCTGACGGCGCTGCGCCGGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAg              <  8:164247/134‑1 (MQ=255)                                                                                                                    ggCGCAACAGCCGCTGACGGCGCTGCGCCGGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGGCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAg              <  7:93116/134‑1 (MQ=255)                                                                                                                      cgcAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGCCGCAGCCt           >  7:250204/1‑135 (MQ=255)                                                                                                                       gcAACAGCCGCGGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTgg         <  4:105022/136‑1 (MQ=255)                                                                                                                        caacccccGCTGACGGCGCGGCGCCTGACCCGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTgg         <  1:78386/129‑1 (MQ=255)                                                                                                                        cAACAGCCGCTGACGGCGCTGCGCCTGACCAGCGTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTgg         <  1:295019/135‑1 (MQ=255)                                                                                                                        cAACAGCCGCTGAAGGCGCTGCGCCGGACCAGCCGGGCCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTgg         <  8:41204/135‑1 (MQ=255)                                                                                                                        cAACAGCCGCGGACGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTgg         <  1:290155/135‑1 (MQ=255)                                                                                                                             gCCGCGGACGCCGCGGCGCCGCCCCCGCCGGGGCGGCCGCGCCCACCACCTGCTGGAACTGGCCGACGGGCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCt    <  1:175338/135‑1 (MQ=255)                                                                                                                               cgcgGAAGGCGCGGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTgg  <  7:175258/135‑1 (MQ=255)                                                                                                                               cGCTGACGGCGCTGCGCCGGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTgg  <  7:183302/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 TCGTCTCCGGGGTTGTCATGCTGTACGTCGGCTACCCCAAGCTGACACCGGCCGAGCGCCTGGCCCACCTGCCGGCTTTGCCGGCGGGGTGCTGCGCCGAGTTGCCCGAGCATTGGGCGCAACAGCCGCTGAAGGCGCTGCGCCTGACCAGCCTGGGCGGGCGCGCGCACCACCTGCTGGAACTGGCCGACGGTCGCCGCGTCATCCTCGATGCCGCCAGCGGCGAGCCTCTGGCTCGGGCCGACGCAGCCTGGGCCCTGG  >  KT2440/3763733‑3763993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑