Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,956,826 G→C 100% H275D (CAT→GAT)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,956,8260GC99.2% 202.5 / ‑6.9 67H275D (CAT→GAT) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (1/2);  major base C (33/28);  minor base A (3/0);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCACCGCGGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATGGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAATGCCCATGCCCA  >  KT2440/3956694‑3956954
                                                                                                                                    |                                                                                                                                
tCACCGCGGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATcgcg                                                                                                                               >  3:14556/1‑136 (MQ=255)
tCACCGCGGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGc                                                                                                                                >  7:110334/1‑135 (MQ=255)
 cACCGCGGCAAACTTCTCGCCTCAGCGCCAATACAGCGCATCATCAACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCATGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATcgcg                                                                                                                               <  1:260448/135‑1 (MQ=255)
     gcgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  4:14556/136‑1 (MQ=255)
     gcgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  4:195944/136‑1 (MQ=255)
      cgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  6:80776/135‑1 (MQ=255)
      cgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  5:125273/135‑1 (MQ=255)
      cgGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  6:2694/135‑1 (MQ=255)
      cgGCAAACTTCTCGCCTCAGCGACAATACCGCGCATCATCCTCCACCCTGCCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCTCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  5:273654/135‑1 (MQ=255)
      cgGCAAACTTCTCGCCGCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATCCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  5:69546/135‑1 (MQ=255)
         cAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGAt                                                                                                                       >  8:345856/1‑135 (MQ=255)
          aaaCTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATa                                                                                                                      <  6:352448/135‑1 (MQ=255)
                 tcGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGc                                                                                                                <  5:150329/134‑1 (MQ=255)
                     cTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCAGGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACa                                                                                                           <  1:125672/135‑1 (MQ=255)
                      tCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAg                                                                                                          >  5:87816/1‑135 (MQ=255)
                         gcgcCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAgcg                                                                                                        <  8:110334/134‑1 (MQ=255)
                                  cagcgcATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTAGCTCCTTCAGCTAGTCGATCCACCTGCGCACCTTGGAGTCCACGTAATACCGGGAGGGATACAACGCAAACAGCGCAATCTCCCg                                                                                              >  5:313355/3‑135 (MQ=255)
                                       catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGc                                                                                          >  8:90721/1‑134 (MQ=255)
                                       catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGc                                                                                          >  8:203675/1‑134 (MQ=255)
                                       catcatCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATAGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGc                                                                                          >  8:130307/1‑134 (MQ=255)
                                        atcatcCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          >  5:278125/1‑101 (MQ=255)
                                        atcatcCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  6:278125/101‑1 (MQ=255)
                                        atcatcCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCg                                                                                        >  1:317061/1‑135 (MQ=255)
                                        atcatcCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCg                                                                                        >  5:69279/1‑135 (MQ=255)
                                                         cATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAAGCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCg                                                                      >  4:37574/1‑136 (MQ=255)
                                                         cATGCCCCTCGAAAAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATACAGGTGCGCACCTTGGCGTCCAGGTAATAGCGTGCCGGCTACATAGCATACCGCGCACGCTCCCGACGCCCATACGGTTCCAATAAcc                                                                       >  5:231550/1‑135 (MQ=255)
                                                           tGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCgg                                                                     >  5:156503/1‑135 (MQ=255)
                                                              cccTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGcac                                                                  >  3:24377/1‑135 (MQ=255)
                                                                cTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACaa                                                                >  5:310326/1‑135 (MQ=255)
                                                                cTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACaa                                                                >  2:200559/1‑135 (MQ=255)
                                                                 tCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACaac                                                               >  2:18329/1‑134 (MQ=255)
                                                                 tCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATAGCATACATAGCACTCTCCCGCAGCCGATACGGTGCAAATAACCGGCACAAGc                                                              >  4:333818/1‑136 (MQ=255)
                                                                     aCAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTcc                                                          >  3:350226/1‑136 (MQ=255)
                                                                            cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCg                                                                                        <  3:236749/99‑1 (MQ=255)
                                                                            cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCg                                                                                        >  4:236749/1‑99 (MQ=255)
                                                                            cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCg                                                   >  3:47983/1‑136 (MQ=255)
                                                                            cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCACCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGccc                                                    >  5:247740/1‑135 (MQ=255)
                                                                            cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGAGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGcc                                                     >  8:291181/1‑134 (MQ=255)
                                                                            cGGCAGTTGCTCCTTGAGGTAGTCGATCCAGCTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCAAACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACAGGCACAAGCGTCCCTg                                                       >  7:252996/1‑132 (MQ=255)
                                                                            cGGCAGTTGCTCCTTGAGGCAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          >  6:204608/1‑65 (MQ=255)
                                                                            cGGCAGTTGCTCCTTGAGGAAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACg                                                                                                                          <  5:204608/65‑1 (MQ=255)
                                                                              gCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTTGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCGGCCCGa                                                  >  1:285036/1‑135 (MQ=255)
                                                                               cAGTTGCTCCTTGAGGTAGTCGAGCCAGGTGCGCACCTTGGCGGCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGAt                                                 <  6:98018/135‑1 (MQ=255)
                                                                                    gcgccTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGAGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCt                                            <  8:328998/132‑1 (MQ=255)
                                                                                      tCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCCCTCTCCCGCAGCCGATACGGTGCCAATACCCGGCACAAGCGTCCCTGCCCGATCGCCTCg                                          >  6:126961/1‑135 (MQ=255)
                                                                                       ccTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGCTACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAATCGTCCCTGCCCGATCGCCTCGc                                         >  6:248828/1‑135 (MQ=255)
                                                                                         ttGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGc                                         <  5:10290/133‑1 (MQ=255)
                                                                                             ggTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGc                                                                                             <  5:46814/77‑1 (MQ=255)
                                                                                             ggTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGc                                                                                             >  6:46814/1‑77 (MQ=255)
                                                                                                aGTCGATCCCGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTa                               <  3:8836/136‑1 (MQ=255)
                                                                                                     aTCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAAc                           >  5:197822/1‑135 (MQ=255)
                                                                                                     aTCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAAc                           >  6:154891/1‑135 (MQ=255)
                                                                                                     aTCCAGGTGCGCACCTTGGCGTCCACGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCACCCGATACGGTGCCAATAACCGGCACAAGCCTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAAc                           >  1:241975/1‑135 (MQ=255)
                                                                                                      tCCAGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACgg                         <  4:339473/136‑1 (MQ=255)
                                                                                                       ccAGGGGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACgg                         <  5:35688/135‑1 (MQ=255)
                                                                                                         aGGTGCGCACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACgg                         <  1:309426/133‑1 (MQ=255)
                                                                                                              cgcACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAg                                                               <  3:304996/90‑1 (MQ=255)
                                                                                                              cgcACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAg                                                               >  4:304996/1‑90 (MQ=255)
                                                                                                              cgcACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGc                 <  4:47983/136‑1 (MQ=255)
                                                                                                               gcACCTTGGCGTCCTGGTAATGGCGTGACGGATACATCGCATACTGCGCACTCTCCCGCAGCCGTTACGGTGCCAATTACCGGCACAAGCGTCCCTGCGCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGc                 <  6:313355/135‑1 (MQ=255)
                                                                                                               gcACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGc                 <  6:87816/135‑1 (MQ=255)
                                                                                                               gcACCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGc                 <  6:310326/135‑1 (MQ=255)
                                                                                                                 aCCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCa               >  2:30925/1‑135 (MQ=255)
                                                                                                                 aCCTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCa               >  5:172367/1‑135 (MQ=255)
                                                                                                                   cTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAat             >  6:367526/1‑135 (MQ=255)
                                                                                                                   cTTGGCGTCCAGGTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAat             <  7:146794/135‑1 (MQ=255)
                                                                                                                              ggTAATGGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGgc                        >  2:335484/1‑113 (MQ=255)
                                                                                                                              ggTAATGGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGgc                        <  1:335484/113‑1 (MQ=255)
                                                                                                                              ggTAATCGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTTCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGTCCAatgcccatgcccc  <  7:291181/135‑2 (MQ=255)
                                                                                                                                    |                                                                                                                                
TCACCGCGGCAAACTTCTCGCCTCAGCGCCAATACCGCGCATCATCCACCACCCTGGCATGCCCCTCGAACAGCGCCGGCAGTTGCTCCTTGAGGTAGTCGATCCAGGTGCGCACCTTGGCGTCCAGGTAATGGCGTGACGGATACATCGCATACAGCGCACTCTCCCGCAGCCGATACGGTGCCAATAACCGGCACAAGCGTCCCTGCCCGATCGCCTCGCTGGCGGTGTAGAACGGCAGCAGGCCAATGCCCATGCCCA  >  KT2440/3956694‑3956954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: