| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,989,982 | A→C | 39.1% | intergenic (+15/+30) | CJ019_03768 → / ← CJ019_03769 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,989,982 | 0 | A | C | 39.1% | 14.3 / 2.7 | 29 | intergenic (+15/+30) | CJ019_03768/CJ019_03769 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/12); new base C (2/10); total (7/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGATTACATCCATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGA > KT2440/3989848‑3990109 | cgcgATTACATCCATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCa < 1:1761/135‑1 (MQ=255)cgcgATTACATCCATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCAAAATGACCCTCGCCGGCCACAg > 3:65496/1‑136 (MQ=255) tACATCCATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTGCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGgcgc < 8:59376/134‑1 (MQ=255) ccATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCCGGCGCCCCcccg > 4:352388/1‑136 (MQ=255) aTGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCgcgc < 1:51852/135‑1 (MQ=255) aCGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGCCATCCTGCCCAAATGACCCTCGAGGGCCGCCCGCGCccccccccccc > 2:338896/1‑134 (MQ=255) gcgTAGTGTCCCCCGGGATGTTCACCGGCTGCCTGTTGGCGGACGGGCTGGGCCCTCCCTTGCGGTTCGACATCGTGCCCAAATCACCCTCGACGGCCCCCGGCGCCCCCTCGCCCGAGGCCGCAAACCGGCAt < 8:106279/134‑1 (MQ=255) cccccGTGAAGTGCTCCGGCGGCCTGAGGGTGGCCGGGCTGGGGCCGGCCTGGGGTTTCCACACCCGCCCCAAATCACCCCCGCCGGCCCCCGGCCCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCa < 2:286493/132‑1 (MQ=255) ccACCGTGATGTGCTCCGGCTGCCTGATGGGGGAGGGGCTGGGGCCTGCAATGCGTTTCGACATCCTGCCCAAATGCCCCTCGCCGGCCTCCGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCa < 8:266489/135‑1 (MQ=255) cACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCGCAGGCGCCCCCCCCCCCCAGGCCGCCAACCCGCATTTTTACTTCa > 2:6532/1‑134 (MQ=255) gTGAAGTTCTCCGGGTGCCGGATGGGGCACGGGCGGGGACCTGCCTTGCGTTTCTCAATCCTGCCCAAAGGACCCTCGACGGCCTCCGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACAcc < 6:87724/135‑1 (MQ=255) gTGAAGTTCTACGGCTGCCTTATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGCCCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACAcc < 7:314668/135‑1 (MQ=255) gAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCGCAGGCCCCCCCCCCCCCGCCGCCCCAAACCCGCATTTTTTCTGGAACACcccc > 4:54635/1‑136 (MQ=255) cGGCTGCCGGATGGGGGACGGGCTGGGGCCTGCCAGGCGTTTCGACATCCTGCCCAAATGCCCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCtt < 4:114350/135‑1 (MQ=255) gaggggCGGGGGCCTGCCATGCGTTGCGACATCCTCCCAAAATGACCCTCGACGCCCTCCGGCGCCCCCGCGCCCGCGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACcgc < 3:186632/133‑1 (MQ=255) aggggCTGGGCCTTGCCATGCGTGTCGACATCCTGCCCAAATGACCCTCCACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACcgc < 6:124650/133‑1 (MQ=255) cTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAg < 7:288257/135‑1 (MQ=255) gggccggCCATGCGTTTCCACATCCTCCCCAAATCACCCTCGCCGGCCCCCGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGcc < 6:2578/129‑1 (MQ=255) ccAGGCGTTGCCACATCCTCCCCAAATCACCCTCCCCGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACa < 7:93190/135‑1 (MQ=255) cATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGc < 3:54635/136‑1 (MQ=255) aTGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGTGGCCGCAAACCGTCATTTTTCCTGCACCACCGCCCTGCCCTTCCCCGCCCTCGGCCCGCGCCCGGCCCAGCACCGc > 3:34071/1‑135 (MQ=255) aTGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCCAAGGCGCCCCCCCCCCCCCGGGCCCCAACCCGCCTTTTTTCTGCCACCACCCCCAGCCCGTCCCACCCCACGGCCCCCGCCCCGCCCCGAAcccc > 4:169892/1‑132 (MQ=255) aTGCGTTTCGACACCTTCCCAAAATGCCCCTCGAGGGCCGCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGc < 7:44163/135‑1 (MQ=255) ccaCAGCCGGCCCAAAGCACCCGCTCCGCCCTCCGCGGCCCCCGCCCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCgg < 6:50440/132‑1 (MQ=255) ccaCAACCTGCCCAAATCCCCCTCGCGGGCCTCCGGCCCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCgg < 2:244643/132‑1 (MQ=255) gaccaccTGCCAAAATGCCCCTCGACGGCTGCCGGCGCCCCCGCGCCCTAGGCCGAAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCTCCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGa < 4:65496/131‑1 (MQ=255) cgggCCTCAGGCGCACCCGCGCCAGTGGCCGAAAACCGTCATTTTTCCTGAAACACCGCCCTTAGCTTCACCGCCCTCGGAACGGGCCAGTCCCAGAAAAGCAAACGGGGTAAGGGTGCGTAGACCACTaataa < 2:88705/132‑4 (MQ=255) ccGGCCCCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGa < 4:222221/135‑1 (MQ=255) cGGCCTCCGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGa < 1:128262/135‑1 (MQ=255) cGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGa < 8:114591/135‑1 (MQ=255) | CGCGATTACATCCATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGA > KT2440/3989848‑3990109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |