Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,029,445 A→C 17.9% Q284P (CAG→CCG)  mcpU_3 → Methyl‑accepting chemotaxis protein McpU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,029,4450AC17.9% 176.7 / 5.2 101Q284P (CAG→CCG) mcpU_3Methyl‑accepting chemotaxis protein McpU
Reads supporting (aligned to +/- strand):  ref base A (34/43);  new base C (14/8);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAG  >  KT2440/4029311‑4029578
                                                                                                                                      |                                                                                                                                     
cATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAg                                                                                                                                      <  4:354324/136‑1 (MQ=255)
cATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAg                                                                                                                                      <  3:64101/136‑1 (MQ=255)
   cAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCCCGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCgcgc                                                                                                                                   >  3:58163/1‑136 (MQ=255)
     aCCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCgcgcg                                                                                                                                  >  7:179857/1‑134 (MQ=255)
     aCCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAgcgc                                                                                                                                   >  5:85536/1‑134 (MQ=255)
     aCCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  4:24270/136‑1 (MQ=255)
     aCCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  4:266574/136‑1 (MQ=255)
     aCCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCAGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAgcgcg                                                                                                                                  >  7:91343/1‑134 (MQ=255)
     aCCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGACATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCAgcgca                                                                                                                                  >  5:182825/1‑134 (MQ=255)
     aCCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCGCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  3:31314/136‑1 (MQ=255)
      ccTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  1:58596/135‑1 (MQ=255)
      ccTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  8:110443/135‑1 (MQ=255)
      ccTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  8:300325/135‑1 (MQ=255)
      ccTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  1:3775/135‑1 (MQ=255)
      ccTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  1:272831/135‑1 (MQ=255)
      ccTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGGCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  6:69457/135‑1 (MQ=255)
        tCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCCCCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCg                                                                                                                               <  5:1808/135‑1 (MQ=255)
           aTAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGAcc                                                                                                                            <  5:355200/135‑1 (MQ=255)
             aGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCATGCCCAGCGCGTCGCCCAg                                                                                                                          >  5:71917/1‑135 (MQ=255)
             aGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGGCCAgg                                                                                                                         >  3:221873/1‑136 (MQ=255)
                cTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTa                                                                                                                       <  6:154209/135‑1 (MQ=255)
                cTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGGCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTa                                                                                                                       <  2:56116/135‑1 (MQ=255)
                  gCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCCAGCTGGCCCAGCGCTTCGACCAGGTAgc                                                                                                                     >  6:144747/1‑134 (MQ=255)
                  gCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCTTCGGCCAGGTAGa                                                                                                                     >  8:360958/1‑135 (MQ=255)
                   cAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGGCTGTGGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGAc                                                                                                                    <  7:111661/135‑1 (MQ=255)
                    aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCAGCCAGCTGGCCCCGCGCGTCGCCCCGGTAGAcc                                                                                                                   >  1:108757/1‑135 (MQ=255)
                    aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCGTGGACCCGGTAGAcc                                                                                                                   >  1:253191/1‑135 (MQ=255)
                    aGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGAcc                                                                                                                   >  7:289127/1‑135 (MQ=255)
                       cACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGa                                                                                                                >  5:75375/1‑135 (MQ=255)
                       cACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCTTCGACCAGGTAGACCCGa                                                                                                                >  8:187557/1‑135 (MQ=255)
                       cACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCAATCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGAc                                                                                                               >  4:65108/1‑136 (MQ=255)
                        aCTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGAc                                                                                                               <  5:85070/135‑1 (MQ=255)
                        aCTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGGCTGTGGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGAc                                                                                                               <  5:89463/135‑1 (MQ=255)
                            aGCCAGGAGGCCAGCCGCAGCCTCTACGACGGCCGCACCACGGTCGGCATTCTCACCCCGGGCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCaa                                                                                                            <  3:76809/134‑1 (MQ=255)
                             gCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCCGCCAGCTGGCCCCGCGCGTCGGCCAGGTAGGCCCGACCaaaa                                                                                                          >  1:347248/1‑134 (MQ=255)
                             gCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCCACCGTGCCCCGCGCGTCGGCCCCGGAGGCCCGCCCCAAg                                                                                                          >  1:351626/1‑135 (MQ=255)
                             gCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGCCCAAAg                                                                                                          >  7:89973/1‑135 (MQ=255)
                             gCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCAGCGCGTCGGCCCGGTAGACCCGCCCAAAg                                                                                                          >  1:211551/1‑135 (MQ=255)
                             gCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 >  4:222583/1‑112 (MQ=255)
                             gCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  3:222583/112‑1 (MQ=255)
                             gCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCaacg                                                                                                          >  8:149773/1‑133 (MQ=255)
                             gCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAg                                                                                                          >  7:116070/1‑135 (MQ=255)
                                 ggaggCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGcc                                                                                                     >  4:294750/1‑136 (MQ=255)
                                     gCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGCTGCCAGCCAGCTGGCCCCGCGCGTCGCCCAGGTAGACCCGCCCAAAGGCGCCgcgc                                                                                                 >  4:265939/1‑136 (MQ=255)
                                     gCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCgcg                                                                                                  >  1:4245/1‑133 (MQ=255)
                                     gCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCTTCGACCAGGTAGACCCGCCCAAAGGCGCCgcg                                                                                                  >  6:354929/1‑133 (MQ=255)
                                       cAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAACCGGGCCCCGCGCTTCGCCCAGGTTTCCCCCCCCAAAcgcgccgcgcg                                                                                                >  8:285414/1‑134 (MQ=255)
                                           cGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGCCCCGACCAAAGGCGCCGAGCTGGTg                                                                                            >  7:26032/1‑135 (MQ=255)
                                           cGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTAGGCTGGCTACAGCGCCGATGCCAGCAAGCAGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCCAGCAGGTg                                                                                            >  6:253279/1‑135 (MQ=255)
                                               gCCTCTACGACGGCCGCACCAGGGGCGGCATTCTCCCCCCGGTCGGGCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGAAGCCCCGCCCAAAGGCGCCGAGCTGGTGCGCa                                                                                        <  6:118752/135‑1 (MQ=255)
                                                       gAAGGCCGCACCACGGTCGGCATTCTCCCCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTTGCCCAGCGCTTCGCCCAGGTAGCCCCGACCAAAGGCGCCGCGCTGGTGCGCAAGCTgggc                                                                                >  7:215926/1‑133 (MQ=255)
                                                         aGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGAt                                                                             <  3:222310/136‑1 (MQ=255)
                                                          ggCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGAt                                                                             <  6:75375/135‑1 (MQ=255)
                                                             cGCACCACGGTCGGCATTCTCCCCCCGGTCGGTATGTTGGCTGGCTACAGCGCCGCTTCCAGCAAGCTGGCCCACCGCGTCGCCCAGGTAGGCCCGCCCAAAGGCGCCGCCCGGGTGCGCCAGCTGGCCGCAGGCa                                                                         >  3:126586/1‑136 (MQ=255)
                                                              gCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 >  7:271619/1‑79 (MQ=255)
                                                              gCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCtt                                                                                                                                 <  8:271619/79‑1 (MQ=255)
                                                              gCACCACGGGCGGCAGTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGGTTCGACCAGGTAGACCCGACCAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCa                                                                         <  7:180181/135‑1 (MQ=255)
                                                               caccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCCAGCTGGGCCCGCGCCTCGCCCCGGTAGACCCCCCCCAAGGCGCCGCGCTGGTGCGCCAGCTGGCCGATGGCaa                                                                        >  6:307775/1‑135 (MQ=255)
                                                                 ccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCTTCGCCCAGGTAGCCCCGCCCCAAGGCGCCGCGCTGGTGCGCAAGCTGGCCGATGGCCAGCt                                                                     >  3:293620/1‑136 (MQ=255)
                                                                 ccacGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGCGCTGGTGCGCAAGCTGGCCGATGGCAAGc                                                                      >  7:37051/1‑135 (MQ=255)
                                                                    cGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGAAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGa                                                                   <  7:245352/135‑1 (MQ=255)
                                                                      ggcGGCTTTCTCACCCCGGTCGGTGTGTTGGCTGGCTACAGCGCCGAGGCCAGCAAGCTGGCCCTGCGCTTGGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGacc                                                                 <  7:108992/133‑1 (MQ=255)
                                                                          gCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCCGCAAGCTGGCCCAGCGCTTCCACCAGGTAGCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAACCGGACCCCCCt                                                            >  4:146486/1‑136 (MQ=255)
                                                                                          cggtcggtGGGCTGGCTACAGCGCCTATGCCAGCAAGCGGGCCCCGCGCTGCGCCCCGGAAGCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAgcgc                                            <  3:139024/136‑1 (MQ=255)
                                                                                           ggtcTGTTGGCTGGCTACCGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAgcgc                                            >  8:91117/1‑135 (MQ=255)
                                                                                           ggtcTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGGAGCACCGCCCAAAGGCCCCCCGCTGGTGCCCACGCTGGCCGCTGGCCAGCGGCCCACCCGCCACGACCGGCAgcgg                                            >  1:279504/1‑134 (MQ=255)
                                                                                           ggtcTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAgcgc                                            >  7:152084/1‑135 (MQ=255)
                                                                                                          tACAGCGCCGATGCCAGCAAGCTGGCCCCGCGCGTCGCCCAGGTAGACCCGCCCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCGCCACGCCCGGCCGCGCGTGAAGGTGCGggcg                             >  7:221473/1‑135 (MQ=255)
                                                                                                          tACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCCGGTAGCCCCGCCCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGCCCACCCTCCCCGCCCGGCAGCGCGTGAAGGTGCTggcg                             >  7:279140/1‑135 (MQ=255)
                                                                                                            cAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGCCCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGCCCACCCGCCACGCCCGGCAGCGCTTGAAGGTGCTggcggc                           >  6:110516/1‑135 (MQ=255)
                                                                                                              gcgcCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGCCCCGACCAAAGGCGCCGCGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGCCCCCCCTCCCCGACCGGCCGCGCGTGAAGGTGCTGGCGGCAt                         >  1:98101/1‑135 (MQ=255)
                                                                                                                 ccGATGCCAGAAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAACGGCGCCGAGCGGGTGCGCAAGGTGGCCGATGGCAAGCTGACAACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTcc                      <  8:301608/135‑1 (MQ=255)
                                                                                                                  cGATGCCAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGCCCCGTCCAAAGGCGCCGCGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGCCCCCCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCg                     >  2:241948/1‑135 (MQ=255)
                                                                                                                     tgcccccAAGCGGGCCCCGCGCTGCGACCCGGTCCCCCCCCCCAACGGCGCCGAGCTGGGGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGcc                  <  6:268710/129‑1 (MQ=255)
                                                                                                                     tGCCAGCACGCTGGCCCAGCGCTTCGACCAGGTAGCCCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGcc                  <  8:289127/135‑1 (MQ=255)
                                                                                                                       ccAGCAAGCTGGCCCAGCGCTTCGCCCAGGTAGACCCGACCAAAGGCGCCGAGCGGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCGCCACGCCCGGCCGCGCTTGCAGGTGCTGGCGGCATTCCGGCCaa                >  6:294484/1‑135 (MQ=255)
                                                                                                                       ccAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCaa                >  2:276373/1‑135 (MQ=255)
                                                                                                                          gaaaGCGGGCCCAGCGCTTCGACCAGGTAGCCCCGACAAAAGGCGCCGAGCGGGTGCGCATGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATcc             <  2:56322/133‑1 (MQ=255)
                                                                                                                              gcgggCCCCGCGCTTCGACCAGGTAGACCCGACAAAAGCCGCGGAGCTGGTGCGAAAGCTGGCCTAGGGCAAGCTCACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  3:294750/133‑1 (MQ=255)
                                                                                                                              gCTGGCCCAGCTGTTCGACCAGGTACACCCTACAAATGGCTCCGAGATGGTGGGCAAGCTGTCCGTTGGCAAGCTGACAACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCACTCCAGGAc        <  4:114634/136‑1 (MQ=255)
                                                                                                                              gCTGGCCCAGCGCTTCGACCAGGTCGACCCGACAAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  4:326246/136‑1 (MQ=255)
                                                                                                                              gCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCt                                                                     <  3:335424/75‑1 (MQ=255)
                                                                                                                              gCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCt                                                                     >  4:335424/1‑75 (MQ=255)
                                                                                                                              gCTGCCCCCGCGCTTCGACCAGGTCGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  4:217550/136‑1 (MQ=255)
                                                                                                                              gCTGCCCCCGCGCTGCGACCTGTTCGACCCGACCATCGGCGCCGTGGTGGTGGGCACGCTGGCCGATGCCAAGCTGACCCCCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  4:286294/136‑1 (MQ=255)
                                                                                                                               gggcccccGCGCTGCCACCAGGACCACCCCACCAACGGCGCCGAGCTGGTGCGCAAGCGGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  7:285414/131‑1 (MQ=255)
                                                                                                                               cgggCCCTGCGCTTCGACGCGGTCGACCCCCCCAAAGGCGCCGAGCTGGTGCGCAAGCGGGCCGATGGCATGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  1:49486/133‑1 (MQ=255)
                                                                                                                               cgggCCCCGCGCTTCGCCCCGGTCCACCCGACCAAGGGCGCCGAGCGGGGGCGCAAGCTGGCCGAGGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  5:110516/133‑1 (MQ=255)
                                                                                                                               cgggCCCAGCGCTTCGACCAGTTAGACCCGACCAACGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  2:2619/133‑1 (MQ=255)
                                                                                                                               cgggCCCAGCGCTTCGACCAGGTCGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  8:61969/133‑1 (MQ=255)
                                                                                                                               cgggCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  2:4245/133‑1 (MQ=255)
                                                                                                                               cTGGCCCAGGGCTTCGACCCGGTAGACCGGACCAACGGCGCCGAGCTTGTGCGCAAGCTGGCCGATGGCAAGCTGACCCCCCTCAACGACCGGCAGCGCTTGGAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  7:279062/135‑1 (MQ=255)
                                                                                                                               cTGGCCCAGCGCTTCGCCCCGGAACCCCCGACCAACGGCGCCGAGCTGGTGCGCACGCGGGCCGATGGCAAGCTGACCACCCGCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  5:144747/135‑1 (MQ=255)
                                                                                                                               cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAACGGCGCCGAGCGGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  2:38850/135‑1 (MQ=255)
                                                                                                                               cTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  8:152084/135‑1 (MQ=255)
                                                                                                                               cTGGCCCAGCGCTGCGACCAGGTAGACCCGACCAAAGGCGCAGAGCTGGTGGGCAAGCTGGCGTAGGGAAAGCTGACCACCCTCCACGACCGGCAGCGCTTGACGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  6:150411/135‑1 (MQ=255)
                                                                                                                               atGGCCCAGCGCTTCGACCTGGTAGACCCGAAAAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGAc        <  7:47630/134‑1 (MQ=255)
                                                                                                                                 ggCCCCGCGGTTCGACCAGGTAGACCCGCCCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGc      <  2:211551/135‑1 (MQ=255)
                                                                                                                                  gCCCCGCGCTTCGACCAGGTAGCCCCGACCAAAGGCGCCGAGCGGGTGCGCAAGCGGGCCGATGGCAAGCTGACCCCCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGc      <  3:298442/134‑1 (MQ=255)
                                                                                                                                     cAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAg  >  5:357445/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CATCAACCTCAATAGCCTGCAGGCACTGAGCCAGGAGGCCAGCCGCAGCCTCTACGAAGGCCGCACCACGGTCGGCATTCTCACCCCGGTCGGTCTGTTGGCTGGCTACAGCGCCGATGCCAGCAAGCTGGCCCAGCGCTTCGACCAGGTAGACCCGACCAAAGGCGCCGAGCTGGTGCGCAAGCTGGCCGATGGCAAGCTGACCACCCTCCACGACCGGCAGCGCTTGAAGGTGCTGGCGGCATTCCGGCCAATCCCGGACGCCCAG  >  KT2440/4029311‑4029578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: