Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,112,358 T→G 21.7% V468G (GTC→GGC)  iorB_2 → Isoquinoline 1‑oxidoreductase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,112,3580TG21.7% 95.7 / 3.2 71V468G (GTC→GGC) iorB_2Isoquinoline 1‑oxidoreductase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (21/31);  new base G (14/4);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGGCCGCCACTTCCTGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCTTGCTGGAAAACAAGGGGAGAAGATCGACCCGACCGCGCTT  >  KT2440/4112224‑4112491
                                                                                                                                      |                                                                                                                                     
cttTGGCCGCCACTTCCTGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGCAGCTTATCTGGAGTCGCGAAGCGGAGTTCTTGCGCGATGTACTGCGCCCCGt                                                                                                                                       >  7:69231/1‑135 (MQ=255)
  ttGGCCGCCACTTCCTGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGTTGAAGCTTATCTGGAGTCGCTAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCg                                                                                                                                     <  1:217564/135‑1 (MQ=255)
  ttGGCCGCCACTTCCTGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTAGCCGGCCGGTGAAGCTTATCTGGAGTCGCGGAGAGGGGGTCTTTGGCGATGGACTGCGCCCCCTCg                                                                                                                                     >  1:48199/1‑135 (MQ=255)
   tGGCCGCCACTTCCTGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCg                                                                                                                                     <  4:73760/134‑1 (MQ=255)
    ggCCGCCACTTCCTGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCg                                                                                                                                     <  5:112800/133‑1 (MQ=255)
       cgccACTTCCTGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAgtg                                                                                                                                <  2:293154/135‑1 (MQ=255)
       cgccACTTCCCGTACGACGCTGCCCACCCCTACCCGCAGGCCATTGCCCTGGCCAACGCGGGTGGCCGGCCGGTGAAGCTTATCTGGCGTCGCGAAGAGGAGTTCGTGCGCGATGTACTGCGCCCCGTCGCAgtg                                                                                                                                <  5:261105/135‑1 (MQ=255)
           aCTTCCTGTACGCCTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGCGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGaa                                                                                                                           <  3:234404/136‑1 (MQ=255)
                 tGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTcc                                                                                                                      <  5:254996/135‑1 (MQ=255)
                      gACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCg                                                                                                                <  4:127299/136‑1 (MQ=255)
                          ctGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAATAGGGGTTCTTTGGCGATGTACTGCGCCCCGGCGCCGTGGTGAAGTTCCGCGCCGGGc                                                                                                             >  2:101334/1‑135 (MQ=255)
                             ccAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGGATCGGTGTTCTTTCTCGGTGTAATTCGCCCCCTCGGAGTGGTGTAGTTACGGGCCCGGCggg                                                                                                          >  5:239455/1‑135 (MQ=255)
                              cAACCCCTACCCGCCGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTAGCTGGAGTCGCGCAGCGGGGTTCTTGGGCCAAGTACTGCGCCCCCGCGCCGTGGGGCAGTTTCGCGCCgggggggcc                                                                                                        >  3:31348/1‑130 (MQ=255)
                              cAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGGAGAGGAGTTATTGCGCGATGTTCAGCGCCCCGGCGCACGGGGGTAGTTTCGCGCCcggggggg                                                                                                         >  2:363282/1‑130 (MQ=255)
                              cAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGATGAGTTGTTGCGGGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGa                                                                                                         >  8:213476/1‑135 (MQ=255)
                              cAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGCTGTTCTGCGCCCCGGCGCAGGGGTGAAGTTGCGCGCCGGGCTggg                                                                                                         >  7:158823/1‑134 (MQ=255)
                               aaCCCCTACCCGCAGGCCTTTGCCCTGGCCAAAGCGGTTGGCGGGCCGGTGAAGCTTATCTGTAGGCGCGACGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGAc                                                                                                        <  5:33366/135‑1 (MQ=255)
                               aaCCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTCCTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGAc                                                                                                        <  8:127871/135‑1 (MQ=255)
                               aaCCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGAc                                                                                                        <  7:239810/135‑1 (MQ=255)
                                aCCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGCTGTACTGCGCCCCGACGCAGTGGTGAAGTTCCGCGCCGGGCTGGCCGc                                                                                                      >  4:109061/1‑136 (MQ=255)
                                 ccccTCCCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCGGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGc                                                                                                      <  6:75101/135‑1 (MQ=255)
                                 ccccTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGc                                                                                                      <  1:159865/135‑1 (MQ=255)
                                        ccGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGTAGAGGAGTTGTTGGGGGCTGTTCTGCGCCCCCGCGCCGTGGTGGAGTTGCGCGCCGGGGGGGGCGCAGCCggg                                                                                               >  2:372470/1‑134 (MQ=255)
                                        ccGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGGAGAGGAGGTGTTGGGCGCTGTACTGCGCCCCCTGGCAGGGGGGTAGTTACCCGCCGGGCTGGGCGCCGCCGGt                                                                                               >  5:120900/1‑135 (MQ=255)
                                        ccGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGCAGAGGAGTTGTTTCGCGGTGTTCTGCGCCCCCGCGCGGGGGGGGAGTTGCGCGCCGGGCGGGGCGCAGGCggg                                                                                               >  1:301871/1‑134 (MQ=255)
                                        ccGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGCAGAGGAGTTCTTGCGCGATGTACTTCGCCCCGTCGCAGTGGTGTTGTTCCGCGGCGGGCGGGACGCACACGGt                                                                                               >  8:319995/1‑135 (MQ=255)
                                        ccGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGCGGACTTCTTGCGCGATGTACTGCGCCCCGGCGGCCGGGGGAAGTTCCGCGCCGGGCGGGCCGCAGCCggg                                                                                               >  8:292873/1‑134 (MQ=255)
                                             ggCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGcc                                                                                          <  1:372470/135‑1 (MQ=255)
                                               ccATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGTTGAGGCGTTCTTGGGCGAAGTCCTGCGCCCCGGCCCACTGGGGCATTTAAGCGCCGGGGTGGCCGCACACGGGATTcccg                                                                                        >  2:332922/1‑133 (MQ=255)
                                               ccATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGGAGAGGGGTTGTTTGGGGCTGTTAGTCGCCCCCGGGCAGGGGGGGAGTTGCGCGCCGGGGGGGGCGGCGCCGGGATGCCact                                                                                       >  4:199252/1‑134 (MQ=255)
                                               ccATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTGCTTGCGCGTTGTACTGCGCCCCGGCGCAGTGGTGTAGTTCCGCGCCCGGCGGGCCGCAGACGGTTTGCCAg                                                                                        >  2:100502/1‑135 (MQ=255)
                                               ccATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGGGAAGCTTATCTGGCGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGGCGCAGACGGTATGCCAg                                                                                        >  2:295162/1‑135 (MQ=255)
                                                       cTGGCCAAAGCGGGTGGCCGGCCGGTGAAGCGTCTCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGGCGCTGTGGTGAAGTTCCGCGCCGGGCGGGACGCAGACGGTATGCCAGTGGCGCTCg                                                                               <  3:167160/136‑1 (MQ=255)
                                                          gCCAAAGCGGGTGGCCGGCCGGTGAAGCTTAGCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAg                                                                             <  6:142428/135‑1 (MQ=255)
                                                            cAAAGCGGTTGGCCGGCCGGTTAAGCTTATCGGGAGTCGCGATGAGGAGTTCTTGCGCCATGTACTGCGCCCCGTCGCTGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGc                                                                           <  6:239455/135‑1 (MQ=255)
                                                                 cGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTGCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAg                                                                     <  3:270170/136‑1 (MQ=255)
                                                                  ggTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGCAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGGCGCAGTGGTGAAGTTCCGCGCCGGGGGGGGCGCAGCCGGTATGCCCGTGGCGCTCGCGGCGGTCAgc                                                                    >  4:99815/1‑136 (MQ=255)
                                                                  ggTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGCAGAGGAGGTGTTGGGCGCTGTTCTGCGCCCCGTGGCAGTGGTGAAGTTCCGCGGCGGGCTGGACGGCGACGGTATGCCACTGGCGCGCGAGGCGGtgcg                                                                     >  1:1077/1‑132 (MQ=255)
                                                                  ggTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGGGCGATGTACTGCGCCCCGTCGCAGTGGGGAAGTTACGCGCCGGGCTGGACGCAGACGGTATGCCACTGGCGCTCGAGGCGGTCAgc                                                                    >  4:165061/1‑136 (MQ=255)
                                                                  ggTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAg                                                                     <  5:138797/135‑1 (MQ=255)
                                                                  ggTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACAGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAgc                                                                    >  3:181100/1‑136 (MQ=255)
                                                                       gccggccgGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGCCGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGccc                                                                >  2:54401/1‑134 (MQ=255)
                                                                       gccggccgGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGGCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCa                                                                >  1:2179/1‑135 (MQ=255)
                                                                       gccggccgGTGAAGCTTATCTGGAGTCGCGAAGAGGAGGTATTGCGCGATGTACTGCGCCCCGTCGCAGTGGGGAAGTTACGCGCCGGGCTGGACGCAGCCGGTATGCCAGTTGCGCGCGCGGCGGGCCGCGCCa                                                                >  5:251602/1‑135 (MQ=255)
                                                                       gccggccgGTGAAGCTTATCTGGAGGCGCGACGAGGAGCTCTTGCGCGATGGACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGcc                                                                 <  8:292959/134‑1 (MQ=255)
                                                                          ggccggTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCAc                                                               >  7:234702/1‑133 (MQ=255)
                                                                           gccggGGAAGCTTAACGGGCGGCGCGACGAGTCGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGa                                                            <  2:338323/135‑1 (MQ=255)
                                                                           cagggTGAAGCTTATCTGGAGGCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAg                                                           <  3:275593/133‑1 (MQ=255)
                                                                            ccggTGAAGGTTCTCTGGCGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTAGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAgg                                                          <  3:205293/136‑1 (MQ=255)
                                                                            ccggTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAgg                                                          <  3:199252/136‑1 (MQ=255)
                                                                             cggTGAAGCTTTTCTGGAGTCGCTACGAGGAGTTCTTGGGCGATGTCCTGCGCCCGGTCGCTGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAgg                                                          <  6:232661/135‑1 (MQ=255)
                                                                             cggTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTCCTGCGCCCCGGCGCAGTGGTGAAGGTCCGCGCCGGGCTGGACGCAGACGGTATTCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAgg                                                          <  2:272098/135‑1 (MQ=255)
                                                                             cggAGAACCTTATCCGGAGTCGCCACGAGTGGTTCTTGCGCGTTTTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCTGGGGGGACCCAGAATGGATGCCAGTGGCGCTCGAGGCGGTCTGAGCCAACGact                                                          <  8:101900/135‑3 (MQ=255)
                                                                              ggTGAAGCTTATCTGGAGGCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAggg                                                         <  8:69231/135‑1 (MQ=255)
                                                                                           tGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCGGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGc                                             <  2:301629/134‑1 (MQ=255)
                                                                                            ggAGTCGCGAAGAGGAGTTCTTTCGCGATGTACTGCGCCCCCGCGGAGGGGTGAAGTTCCGCGCCGGGCGGGACGCAGCCGGTATTCCCGTGGCGCTCGAGGCGGTCAGCGCCCCCCAGGGCCCAACCCAGGccag                                          >  4:275593/1‑134 (MQ=255)
                                                                                             gAGTCGCGCAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTGCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCCAGGGCCCCACCGAGGcccg                                          >  7:248521/1‑134 (MQ=255)
                                                                                             gAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTACGCGCCGGGCTGGCCGCAGACGGTATGCCCGTGGCGCTCGAGGCGGTCAGCGCCACCCAGGGCCCAACCGAGGCCCt                                          >  5:160852/1‑135 (MQ=255)
                                                                                              aGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTTAAGTGCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGccc                                           <  7:259331/133‑1 (MQ=255)
                                                                                                tCGCGAAGAGGAGTGCTTGCGCGATGTACTGCGCCCCGTCGCTGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCTTGc                                       <  4:167413/135‑1 (MQ=255)
                                                                                                 cgcgTAGAGGAGTTCTTGCGCGATGTACTGCGCCCCCTCGCAGTGGTGTAGTTGCGCGCCGGGCTGGGCGCAGACGGTATGCCAGTTGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGCGGCCCGTGCt                                      >  2:61601/1‑135 (MQ=255)
                                                                                                  gcgGAGAGGTGTTGTTGCGCGATGTACTGCGCCCCGGCGCAGTGGTGGAGTTGCGCGCCGGGCTGGACGCAGACCGTATGCCCGTGGCGCTCTAGGCGGTCAGCGCCCCCGCGGGCCCCCCCCAGGCCCGTGCggg                                    >  4:250165/1‑136 (MQ=255)
                                                                                                  gcgAATAGGAGTTATTGCGCGATGTACTGCGCCCCGTCGCAGTGGTTAAGTTCCCCGCCGGGCTGGAAACAGACCGTATTCCACTGGCGCTATAGGCGGTCAGCGCCACCGCCGGGCCCACCCAGGCCctggctgg                                    >  4:324552/1‑136 (MQ=255)
                                                                                                   cgAAGAGGAGTTCTTTCGCGATGTACAGCGCCCCGTCGCAGTGGTGAAGTTACGCGCCGGGCTGGTCGCACACGGTTTGCCAGTGGCGCACGAGGCGGTCCGCGCCACCGAGGGCCCCCCCGCGGCCCATGCTg                                     >  5:130659/1‑134 (MQ=255)
                                                                                                     aaGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCTTGCTGGaa                                  <  1:363282/135‑1 (MQ=255)
                                                                                                       gagtagTTCTTGCGCGATGTACTGCGCCCCGGCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCTTGCTGGaaaa                                <  5:304697/135‑1 (MQ=255)
                                                                                                           agTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGGGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCTTGCTGGAAAACAAgg                           <  3:104681/136‑1 (MQ=255)
                                                                                                                     cgATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCTTGCTGGAAAACAAGGGga                        >  6:348281/1‑129 (MQ=255)
                                                                                                                          tACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCCCCGAGGGCCCAACCGAGGCCCTTGCTGGAAAACAAGGGGAGAAGATcgacc             >  1:159942/1‑135 (MQ=255)
                                                                                                                          tACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCGTGCTGGAAAACAAGGGGAGAAGATcgacc             >  1:159940/1‑135 (MQ=255)
                                                                                                                                  cccGGCGCTGTGGTGAAGTTCCGCGCCGGGCTGTACGCAGAGGGAATGCCAGTGGCGCTCGGGGAGGTAAGCGCCACCGTGGGCCAAACCGAGGCCCTTGCTGAAAAACAAGGGGAGAAGATCGACCCGACCgcgc    <  4:330567/136‑1 (MQ=255)
                                                                                                                                     gTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCTTGCTGGAAAACAAGGGGAGAAGATCGCCCCGACCGCGCtt  >  1:101814/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CTTTGGCCGCCACTTCCTGTACGACTCTGCCAACCCCTACCCGCAGGCCATTGCCCTGGCCAAAGCGGTTGGCCGGCCGGTGAAGCTTATCTGGAGTCGCGAAGAGGAGTTCTTGCGCGATGTACTGCGCCCCGTCGCAGTGGTGAAGTTCCGCGCCGGGCTGGACGCAGACGGTATGCCAGTGGCGCTCGAGGCGGTCAGCGCCACCGAGGGCCCAACCGAGGCCCTTGCTGGAAAACAAGGGGAGAAGATCGACCCGACCGCGCTT  >  KT2440/4112224‑4112491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑