Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | KT2440 | 4,135,607 | A→C | 49.6% | Q32P (CAG→CCG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | KT2440 | 4,135,607 | 0 | A | C | 49.6% | 6.0 / 34.1 | 63 | Q32P (CAG→CCG) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
Reads supporting (aligned to +/- strand): ref base A (14/17); new base C (24/8); total (38/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.11e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 |
AAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTG > KT2440/4135473‑4135740 | aaGCCCGCACCCTCCCCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 2:211306/135‑1 (MQ=255) cccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCCGCCACCGCCCAG‑GCCTGGgcg > 4:119615/1‑136 (MQ=255) cccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTGCGTTTCCAGATGTGCAGCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAG‑GCCTGGgcg < 4:336304/136‑1 (MQ=255) ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAC‑GCCTGGgcg > 1:291030/1‑135 (MQ=255) cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCTACAGCCTCAACCCGGCCACCGCCCCG‑GCCAGGGCGCCACGGc > 4:328386/1‑136 (MQ=255) gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCCG‑GCCGGGGCGCCCCTGcc > 3:150777/1‑136 (MQ=255) gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCAGGGCGCCACTGc > 8:88563/1‑135 (MQ=255) ccGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCC‑GCCTCGGCGCCACTGCCCgcc > 4:110492/1‑134 (MQ=255) gCCAAGGGGATTGTTGCATTACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCcgccgc > 4:334103/1‑136 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑CCCGGGGCGCAACTGCCCGACGc > 3:158473/1‑136 (MQ=255) aaGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGCCGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCG‑GCCAGGGCGCACCTGCCCGACGcc > 4:237448/1‑134 (MQ=255) ttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACAGCCCGCCGCCGCTGAAGCCGa > 7:116923/1‑135 (MQ=255) tgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCTGGGCGCAACAGCCCGCCGCCGATGAAGCCGag > 6:337659/1‑134 (MQ=255) tgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCGGGGCGCCACAGACCGCCGCCGATGAAGCCGag > 5:70617/1‑134 (MQ=255) gctccccccTCGTGCTCTACCTGATGTGCATTGACGGCAACGGGGGCTAGCCCCATCGCGTCATGACTATCGACCGCCACAACCCGCCCACC‑CCCAGCGCCTGGCCGCAACTGCCCGACGCCGATGAAGCCGAtgt < 4:311732/130‑1 (MQ=255) aCCCTCGTGCGATGCCAGATGGGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACATTCGACACCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAg < 5:165505/135‑1 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCTGGGCGCCACGGCCCGACGCCGATGAAGCCGATGTGGAGc > 2:145233/1‑135 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCC‑GCCGAGCCGCACCAGACCCACCCCGACGAAGCCGCAGTGGAGc > 7:310688/1‑135 (MQ=255) cgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCGCCCCG‑GCCTGGGCGCAACTGCCCGACGCCGCAGAAGCAGATGTGGAGCTTGCCGtt > 8:346073/1‑135 (MQ=255) cgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCTGGGCGCCACGGCCCGCCGCCGAGGAAGCCGATGTGGCGCTGGCCCtt > 6:286721/1‑135 (MQ=255) cgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGACCATGCAGCCGATGTGGAGCTTGCCGt > 8:306309/1‑134 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCC‑GCCTGGGCGCAACAGCCCCACCCCGATGAACCCGATGTGGAGCTGGCCGTTCACgc > 2:52217/1‑135 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGgcc > 4:89329/1‑136 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCAC‑CCCAGGGCGCAACTGCCCGACGCCGATGCAGCCCCTGTGGAGCTGGCCCTTCAGgcc > 4:122856/1‑136 (MQ=255) ccAGAGGTGCCTCGACGGCCACGGGCGCGATGCCCAGCGCGGCAAGACCTTCGACAGCCTCAACCCGCCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAgg < 5:115361/134‑1 (MQ=255) cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCC‑GCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGgccc > 4:206445/1‑135 (MQ=255) cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCC‑GCCGGGGCGCCACTGCCCGCCGCCGCTGGAGCCGATGTGGAGCTGGGCGTTCAGgcc > 8:3638/1‑135 (MQ=255) aCGGCCAATGGCGCGAGGCCCAGAGCGGCAAGACCTTCGCCAGCCTCACCCCCGCCCCCGCCCCG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCtt < 3:254904/136‑1 (MQ=255) cgcccAAGGGCGCGATCCCCAGCGCGCCAACCCCTTCCCCACCCACACCCCGCCCCCCCCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCtt < 7:6003/132‑1 (MQ=255) cGGCCACGGGCGCGAGGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCCG‑GCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCtt < 7:301830/135‑1 (MQ=255) ctgggCGCGAGGCCCCGAGCGCCAACACCTCCCACCCCCCCACCCCGCCCCCCGCCCCG‑GCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa < 5:101406/132‑1 (MQ=255) accggCGCGAGGCCCGGCGCGGCAAGACCTTCGACAGCCGCACCCCGGCCCCCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa < 5:300508/132‑1 (MQ=255) gcgcGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAG‑GCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg < 4:114565/136‑1 (MQ=255) gcgcGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCTGGGCGCCACTGCCCGCCGCCGATGACGCCGATGTGGCGCTGGCCGTTCAGGCCGCCCCGCGTGCCTTCGCCAGCAAg > 4:31547/1‑136 (MQ=255) cgATGCCCAGAGCGGCAAGACCTGCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCg < 4:66373/136‑1 (MQ=255) gaggCCCAGAGCGCCACGCCCTCCCCCAGCCCCACCCCGCCCCCCCCCCCG‑GCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCg < 1:5067/132‑1 (MQ=255) aTGCCCAGAGCGGCAAGACCTTCGACAGCTTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 4:109785/136‑1 (MQ=255) tGCCCAGCGCGGAACGACCTTCCACAGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 6:337687/135‑1 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACTCGGCCACCGCCCCG‑GCCGGGGCGCACCTGCCCGGCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCGTGCCGTCGCCCTCAAGGCGTg > 8:324976/1‑135 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCTGGGCGCCACAGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGTTGAGGCCGCCCCGCGTGCCGTCGACACCCAGGCGTgg > 3:225414/1‑136 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCC‑GCCAGGGCGCAACTGCCCGACGCCGATGAAGCCGCTGTGGCGCTGGGCGTTCAGGCCGCCCACCGTGCCGTCGCCAGCAAGGCGTgg > 4:260155/1‑136 (MQ=255) tGCCCAGAGCGCCAAGACCTGCGCCCGCCCCACCCCGCCCACCGCCCCG‑GCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 2:193907/135‑1 (MQ=255) ccAGAGCGGCAAGACCTTCGACCGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGgcgc < 4:59362/136‑1 (MQ=255) cAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGgcgc < 8:357784/135‑1 (MQ=255) gccgCAAGACCTGCGACAGCCACCACCCGCCCACCCCCCAG‑CCGGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcagca < 1:18663/132‑1 (MQ=255) ggCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCTGGGCGCCACTGCCCGCCGCCGCGGCAGCCGCTGTGGAGCTGGCCGTTCAGGCCCCCCCGCGTGCCTTCGACACCAAGGCGTGGCGCAGCATc > 1:105563/1‑135 (MQ=255) acaCCTGCGACAGCCACAACCCGCCCACCCCCCCG‑GCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCGTCACCg < 8:12183/133‑1 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCCCCGCCCCG‑GCCTGGGCGCAACTGCCCGACGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCCGCCTCACCg > 7:280206/1‑134 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCGGGGCGCAACAGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGGCGGTGAGGCCGCCCAGCGTGCCGTCGACAGCAAGGCGGGGCGCAGCATCACCGc > 4:140974/1‑135 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCGCCCAC‑GCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCCCTGTGGAGCTGGCCGTTCAGGCCGCCCCGCCTGCCTTCGCCACCAACGCGTGGCGCAGCCACAccc > 6:180490/1‑133 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACAGCCCCG‑GCCTCGGCGCAACAGCCCGACGCCCATGAAGCCCATGTAGAGCATGACGATAATGCCCCCCAGCGTGCCATCGACAGCAAGGCGTGTCGCAGCATAAACGc > 8:161813/1‑135 (MQ=255) tgcGACAGCCTCAACCCGGCCACCGCCCAG‑GCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc < 6:64282/133‑1 (MQ=255) tCGACAGCCTCAACCCGGCCACCGCCCCG‑GCCTGGGCGCAACTGCCCGACGCCGCTGAAGCCGATGTGGAGCTGGCCGGTCAGGCCGCCCAGCGTGGCGTCGCCCGCAAGGCGTGGCGCCGCATCACCGCTAccgc > 4:302605/1‑136 (MQ=255) aCAGCCTCAACCCGGCCACCGCCCAG‑GCCGGGGCGCCACTGCCCGCCGCCGAAGAAGCCCATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGGCAGCAAGGCGTGGCGCCGCCTCACCGCTAcccccc > 2:319962/1‑135 (MQ=255) cAACCCGGCCACCGCCCCG‑GCCTGGGCGCCACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCGGGCCGTCGCCACCAACGCCTGGCGCAGCAACACCGCAACCCCCCCCGGccc > 6:11169/1‑133 (MQ=255) ccGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctg < 1:116873/135‑1 (MQ=255) cgccccccGCCCCG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 4:159922/130‑1 (MQ=255) gcccACCGCCCCG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 8:54465/133‑1 (MQ=255) aCCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGcgt < 8:95567/135‑1 (MQ=255) aCCGCCCAG‑GCCTGGGCGCAACGGCCCGACGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCGTGCCGTCGCCAGCAAGGCGTGGCGCAGCATCACCGCGACCGCCCGCGGCAAGCGGCTGcgg > 1:268598/1‑134 (MQ=255) aCCCCCCAG‑CCCTGGGCGCAACTGCCCCACGCCCGTGGAGCCGATGTGGCGCTGGGCGTTCAGGCCGCCCAGCGTTCCGTGGACAGCCAGGGGTGGCGCCCCATCACCCCCACCCCCCCCCGCAAgcggcgggggc > 4:5259/1‑130 (MQ=255) cccAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCACCGTGCCGTCGCCAGCAAGGCGTGGCGCAGCATCACCCCTACCGCCCCCCGCCAgcggcggcggcggg > 8:74900/1‑133 (MQ=255) ccAG‑GCCTGGGCGCAACTGCCCGACGCCGAAGAAGCCGATGTGGAGCTGGCCGGTCAGGCCGCCCAGCGTGCCGTCGACAGCAACGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCACGCGGCTGCGTCGTCt > 8:356237/1‑135 (MQ=255) cAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTg < 8:63710/135‑1 (MQ=255) | AAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAG‑GCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTCTG > KT2440/4135473‑4135740 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |