Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,151,360 A→C 15.2% V55G (GTG→GGG)  cmpR_5 ← HTH‑type transcriptional activator CmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,151,3600AC15.2% 192.3 / 3.6 96V55G (GTG→GGG) cmpR_5HTH‑type transcriptional activator CmpR
Reads supporting (aligned to +/- strand):  ref base A (33/45);  new base C (13/5);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 3.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGTACTGGCGCCGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGTTGCCGTGTTCGGCAACCGTGG  >  KT2440/4151225‑4151495
                                                                                                                                       |                                                                                                                                        
gTGGTACTGGCGCCGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCa                                                                                                                                          >  4:244624/1‑136 (MQ=255)
  ggTACTGGCGACGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCAcg                                                                                                                                        <  3:125345/136‑1 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCCGCCGCACGCCGcggccg                                                                                                                              >  2:269131/1‑135 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCCCGCCGcggccg                                                                                                                              >  6:237734/1‑135 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccgg                                                                                                                             <  3:63530/136‑1 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccg                                                                                                                              >  8:270983/1‑135 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccg                                                                                                                              <  8:200149/135‑1 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccg                                                                                                                              >  1:238943/1‑135 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccg                                                                                                                              >  1:59850/1‑135 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccg                                                                                                                              >  6:158522/1‑135 (MQ=255)
            ccGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCCCCCAGCGCGTGCGCCGGCCGGAAGGTTTCGCGCCGGCGCGCGGCAGGCGCCAGCAACTGCTCCCCGGCCGCTCTCAGCCCCCCCCCGcggccc                                                                                                                              >  5:255937/1‑134 (MQ=255)
              gATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccggc                                                                                                                            <  2:353592/135‑1 (MQ=255)
              gATGCGCACGGGGCCGCGCGCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCa                                                                                                                           <  4:237963/136‑1 (MQ=255)
                 gcgcACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCATCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGACAGCAACTGCTACCCGGCCGCTGTAAGCCGCACGCCGCGGCCGGCCCgg                                                                                                                        >  3:221075/1‑135 (MQ=255)
                 gcgcACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGCCGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCCCg                                                                                                                         >  7:215102/1‑135 (MQ=255)
                 gcgcACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCCCCGGCCGCGGTCAGCCGCACGCCGCGGCCGGCCCGt                                                                                                                        >  4:136845/1‑136 (MQ=255)
                 gcgcACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCCCGCCGCGGCCGGCACg                                                                                                                         >  8:75847/1‑135 (MQ=255)
                 gcgcACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccggc                                                                                                                            <  4:21114/132‑1 (MQ=255)
                 gcgcACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGcggccggc                                                                                                                            >  3:21114/1‑132 (MQ=255)
                 gcgcACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACg                                                                                                                         >  5:127077/1‑135 (MQ=255)
                    cACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTg                                                                                                                      <  2:278841/135‑1 (MQ=255)
                      cGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGAGCAGCGCGTGCGCCTGACGGACGCTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGt                                                                                                                    <  2:130468/135‑1 (MQ=255)
                          gCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAgg                                                                                                               <  3:39667/136‑1 (MQ=255)
                          gCCGCGCTCCAGCCAGCGCATGGCCTCGCGCAGCGCTTTCGCCTGACGGAAGGTGTCGCGCAGGCGCTCGGCATGCGAGAGCAACTGCGCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAg                                                                                                                <  6:211807/135‑1 (MQ=255)
                            cgcgCTCCAGCCCGCGCATGGCCTCGCGCCGCGCTTGCGCCGGACGGAAGGTTTCGCGCAGGCGCTCGGCAAGCGCCCGCAACTGCTCACCGGCCGCGGTCCGCCGCCCGCCGCGGCCCGCCCGGTGGGAAAggg                                                                                                              >  2:137983/1‑135 (MQ=255)
                            cgcgCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCCGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCGGTCACGCGCACCCCGCGGCCCGCCACGTGGGAAAggg                                                                                                              >  1:153703/1‑135 (MQ=255)
                            cgcgCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCCCGCCCCGGCCGGCACGTTGGTAAAgggg                                                                                                             >  4:271536/1‑136 (MQ=255)
                                 tCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCCGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCg                                                                                                         >  8:132710/1‑135 (MQ=255)
                                   ccgCCCGCGCTTGGCCGCGCGCTGGGCTTGCGCGTGAGGTTTGGTTGCGCGGTGGCGCTCGGCTTGCGCACTAAACTGCTCACGGGCCGCTGTCAGCCGCCGGCCGCGGCGGGCACGTTGGTAAAGGGGCTCGcc                                                                                                       <  2:352159/133‑1 (MQ=255)
                                    aGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGc                                                                                                        <  1:16165/133‑1 (MQ=255)
                                      cccGCTCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCCGGCGCTAGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCCCACGCCGCGGCCGGCACGTTGGTAAAGGGGCGCGCCaaa                                                                                                    >  8:22327/1‑135 (MQ=255)
                                      cccGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCCGCCGCCCGCCGCGGCCGGCACGTGGGTAAAGGGGCGCGcccca                                                                                                    >  5:27132/1‑132 (MQ=255)
                                      cccGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCCGCCGCACGCCGCGGCCGGCACGTTGGGAAAGGGGCTCGCCAAAc                                                                                                   >  4:184923/1‑136 (MQ=255)
                                      cccGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCGCGCCacac                                                                                                   >  4:227099/1‑136 (MQ=255)
                                      cccGCGCATGGCCTCGCGCAGCGCTTGCGCCGGCCGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCACCTGCTCACCGGCCGCTGTCAGCCGCCCGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCcaa                                                                                                    >  1:9745/1‑135 (MQ=255)
                                             aTGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCCCGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCctct                                                                                             >  7:242139/1‑135 (MQ=255)
                                             aTGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAggg                                                                                                              >  8:344984/1‑118 (MQ=255)
                                             aTGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAggg                                                                                                              <  7:344984/118‑1 (MQ=255)
                                              tGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCtt                                                                                            <  2:63093/135‑1 (MQ=255)
                                              tGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCtt                                                                                            <  2:320762/135‑1 (MQ=255)
                                              tGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCtt                                                                                            <  8:292814/135‑1 (MQ=255)
                                                        cAGCTCTTGCGCCTGACGGACGGTTGCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTAACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTg                                                                                 <  3:361323/136‑1 (MQ=255)
                                                        cAGCGCTTGCGCCTGACGGAAGGGTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTg                                                                                 <  4:78862/136‑1 (MQ=255)
                                                         aGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTg                                                                                 <  8:202718/135‑1 (MQ=255)
                                                              ttGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCCGCCGCACGCCGCGGCCGGCCCGTTGGGAAAGGGGCTCGCCACACGCCTCTTGCAAGAGCTTGAGCTgg                                                                           >  3:272972/1‑136 (MQ=255)
                                                              ttGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTgg                                                                           >  4:358553/1‑136 (MQ=255)
                                                              ttGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTgg                                                                           >  3:256279/1‑136 (MQ=255)
                                                              ttGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCGGTCCGCCGCCCGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCGCTTGCAAGAGCTTGAGCTg                                                                            >  1:209696/1‑135 (MQ=255)
                                                                       acggacggTTTCGCGCCGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAACGGGCCGCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGaca                                                                    <  4:48819/134‑1 (MQ=255)
                                                                        cGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAccg                                                                 >  4:20740/1‑136 (MQ=255)
                                                                        cGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAcc                                                                  >  2:301682/1‑135 (MQ=255)
                                                                        cGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAcc                                                                  >  6:110636/1‑135 (MQ=255)
                                                                        cGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCCGCCGGCACGTTGGGAAATGGGCTCGCCCAACGCCTCTTTCAAGAGCTTGAGCTGGCCggtcccc                                                                  >  5:263464/1‑130 (MQ=255)
                                                                         ggAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCACACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAccgc                                                                >  3:278643/1‑136 (MQ=255)
                                                                          gAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAccgcc                                                               >  3:133249/1‑136 (MQ=255)
                                                                           aaGGTCTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAACGGGGCGCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAccgccg                                                              <  3:302730/136‑1 (MQ=255)
                                                                            cggTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCCCGCCGCGGCGGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAccgccg                                                              <  7:346034/134‑1 (MQ=255)
                                                                            aGGTTTCGCGCAGGCGCGCGGCATCCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAACGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAccgccg                                                              <  7:312542/135‑1 (MQ=255)
                                                                             ggTTGCGCGCAGGCGAGCGGCCTGCGCCAGCAACTGCTCACCGGCCGCTGTCCGCCGCACGCCGCGGCCGGCCCGTTGGGAAAGGGGCTCGCCAACCGCCTCTTGCAAGAGCTTGAGCTGGCCGGACAccgccg                                                              <  8:174244/134‑1 (MQ=255)
                                                                              gTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGc                                                            <  8:271458/135‑1 (MQ=255)
                                                                                ttCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCCCGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACCCCGCCGGc                                                            >  8:295808/1‑133 (MQ=255)
                                                                                cgcgcgCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCGCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTg                                                          <  1:254323/133‑1 (MQ=255)
                                                                                 tCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  3:20740/135‑1 (MQ=255)
                                                                                 tCGCGCAGGCGCTCGGCAGGCGCCAGCAACTGCTCACCGGCCGCGGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  5:237734/135‑1 (MQ=255)
                                                                                  cgcgCCGGCGCTCGGCATGCGCCAGCAACTGCGCACCGGCCGCTGGCCGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  2:313992/134‑1 (MQ=255)
                                                                                  cgcgCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  2:238943/134‑1 (MQ=255)
                                                                                  cgcgCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  5:110636/134‑1 (MQ=255)
                                                                                  cgcgCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  8:242139/134‑1 (MQ=255)
                                                                                  cgcgCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  7:244767/134‑1 (MQ=255)
                                                                                  cgcgCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCGGGCAGCCGCACGCCGCGGCCGGCACGTTGGTAACGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  2:255485/134‑1 (MQ=255)
                                                                                  cgcgCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGATGTCAGCCGCACGCCGCGGCGGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  5:349017/134‑1 (MQ=255)
                                                                                  cccgcCGGCGCTCGCCTGGCGCCAGCACCGGCGCCCCGGCCGCGGACCGCCGCCCGCCGCGGCCGGCACGTTGGTAACGGGGCGCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTga                                                         <  5:9323/132‑1 (MQ=255)
                                                                                     gcAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCCCGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACa                                                     >  2:106603/1‑135 (MQ=255)
                                                                                     gcAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGaca                                                                    >  3:260125/1‑120 (MQ=255)
                                                                                     gcAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGaca                                                                    <  4:260125/120‑1 (MQ=255)
                                                                                       aGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAgg                                                   <  6:128374/135‑1 (MQ=255)
                                                                                       aGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAgg                                                   <  2:211729/135‑1 (MQ=255)
                                                                                       aGGCGCGCGGCATGCGCAAGCAACTGCTCACCGGCCCCTGCCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCGCCCCAAACCCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGt                                                  <  4:289720/136‑1 (MQ=255)
                                                                                            cTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACCCCGCCGGCTGAGACAGGTGCAg                                              >  7:140391/1‑135 (MQ=255)
                                                                                              gggCTTGCGCCAGCAACGGCGCCCCGGCCGCGGTCCGCCGCACGCGGCGGCCGGCACGTGGGTAAAGGGGCTCGCAAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGc                                           <  3:271536/135‑1 (MQ=255)
                                                                                               ggCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCCCGCCGCGGCCGGCCCGTTGGGTAAGGGGCTCGCCCAACGCCGCTTGCCAGAGCTTGAGCTGGCCGGACACCGCCGGCGGAGCCAGGTGCAGGGCt                                          >  3:47691/1‑136 (MQ=255)
                                                                                                    gcgcCAGCAACTGCTCACCGGCCGCTGTCTGCCGCCGGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCAAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGc                                     <  3:124360/136‑1 (MQ=255)
                                                                                                      gcCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGACTATTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGc                                     <  8:227236/134‑1 (MQ=255)
                                                                                                        cAGCAACTGCTCACCGGCCGCTGTCCGCCGCCCGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCGTGAGCTGGCCGGCCACCGCCGGCTGAGCCAGGTGCAGGGCTTGGGcc                                    >  1:105173/1‑133 (MQ=255)
                                                                                                        cAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACCCCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGc                                  >  8:188275/1‑135 (MQ=255)
                                                                                                          gCAACTGCTCACGGGCCGCTGTGAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCAt                                <  8:62556/135‑1 (MQ=255)
                                                                                                          gCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCAt                                <  8:149644/135‑1 (MQ=255)
                                                                                                            aaCTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATgg                              >  8:273178/1‑135 (MQ=255)
                                                                                                                 cTCCCCGGCCGCGGTCAGCCGCACGCCGCGGCCGGCACGGTGGGAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATg                        <  3:227099/136‑1 (MQ=255)
                                                                                                                    aCCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACCCCGCCGGCTGAGCCAGGTGCAGGGCTTGGGCCGCATGGCTGATGtt                      >  8:178308/1‑135 (MQ=255)
                                                                                                                    aCCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGtt                      <  6:124948/135‑1 (MQ=255)
                                                                                                                    aCCGGACGCTGTCAGCCGCACGCCGCGGCCGGCACGATGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCAGAGACAGGTCCAGGGCTTGGGCCGCATGGCTgttgtt                      >  6:44165/1‑135 (MQ=255)
                                                                                                                     ccGGCCGCGGGCAGCCGCCCGCCGCGGCCGTCACGGTGGTAACGGGGATCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGTTg                     <  6:255937/135‑1 (MQ=255)
                                                                                                                     ccGGCCGCGGGCAGCCGCACGCCGGGGCCGGCCGGGTGGTAAAGGGGCGCGCCAAACGCCTCTTGCACGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGTTg                     <  8:319785/135‑1 (MQ=255)
                                                                                                                         ccGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGTTGCCgt                 >  2:83550/1‑135 (MQ=255)
                                                                                                                              gTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGTTGCCGTGTTCg            >  8:119875/1‑135 (MQ=255)
                                                                                                                               tCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGTTGCCGTGTTCgg           <  2:198323/135‑1 (MQ=255)
                                                                                                                                       aCGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGTTGCCGTGTTCGGCAACCGTgg  <  3:81192/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GTGGTACTGGCGCCGATGCGCACGGTGCCGCGCTCCAGCCCGCGCATGGCCTCGCGCAGCGCTTGCGCCTGACGGAAGGTTTCGCGCAGGCGCTCGGCATGCGCCAGCAACTGCTCACCGGCCGCTGTCAGCCGCACGCCGCGGCCGGCACGTTGGTAAAGGGGCTCGCCAAACGCCTCTTGCAAGAGCTTGAGCTGGCCGGACACCGCCGGCTGAGACAGGTGCAGGGCTTGGGCCGCATGGCTGATGTTGCCGTGTTCGGCAACCGTGG  >  KT2440/4151225‑4151495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: