Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,187,960 C→T 5.2% intergenic (‑239/+249) CJ019_03937 ← / ← CJ019_03938 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,187,9600CT 5.2% 239.2 / 3.2 77intergenic (‑239/+249)CJ019_03937/CJ019_03938hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/44);  new base T (2/2);  total (31/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

CGTTCAAGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAGCACCCTCA  >  KT2440/4187827‑4188094
                                                                                                                                      |                                                                                                                                      
cGTTCAAGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGcgt                                                                                                                                      >  4:254770/1‑136 (MQ=255)
cGTTCAAGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGcg                                                                                                                                       >  8:119258/1‑135 (MQ=255)
   tCAAGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGcgtcg                                                                                                                                    >  1:178193/1‑135 (MQ=255)
    cAAGGTCAGCCATCGCCTGCTACCACTTTCCAAGAAAC‑TCCTCCCAGTCATCAAAATCGGCGCGTGCAGCTACCGGGGTTGAGCCCAAGCTTGCGCACAAAAAACGCCCACGCCACGGCTCCGGTATAGcgtcgt                                                                                                                                   <  3:28743/135‑1 (MQ=25)
     aaGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCACGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCgtcgtc                                                                                                                                  >  1:78130/1‑135 (MQ=255)
     aaGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCCCGGCTCCGGTATAGCgtcgtc                                                                                                                                  >  5:56190/1‑135 (MQ=255)
     aaGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCgtcgtc                                                                                                                                  >  1:161661/1‑135 (MQ=255)
     aaGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCgtcgtc                                                                                                                                  >  7:181451/1‑135 (MQ=255)
     aaGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCgtcgtc                                                                                                                                  >  6:129926/1‑135 (MQ=255)
       gggtaGCCATCTCGTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTccc                                                                                                                                <  8:123369/131‑1 (MQ=255)
         tgcgCCTTCTCGTGCTTCCACTTTGCAAGAAAC‑TTCACCCTGTCATGCAAATAGGCGCCTTAAGCTACCGGGGTTGTGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGt                                                                                                                              <  1:70264/132‑1 (MQ=25)
         tgccccATCCCCGGCTGCCAC‑TTGCAAAAAACTTTGCCCCCGCCATGAAAATCGGCGCATGCCGATACCGGGGTTGCGCCAACGCTTGCCCACAAAAACCGCCCACGCCACGGCTCCGGTATAGCGTCGTCCCGt                                                                                                                              <  2:178193/131‑1 (MQ=9)
         tGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGt                                                                                                                              <  2:18849/135‑1 (MQ=255)
              cATCTCGTGCTTCCAGTTTGCAAGAAAC‑TTCTCCCCGTCATGCAAATAGGCCCCTTCAGGTACCCGGGTGGACCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGAt                                                                                                                         <  1:116455/135‑1 (MQ=25)
               aTCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCTCACACAAAACGCTCACGCCACGGCTCCGGTATAGTGTCGTCCCGTACGAt                                                                                                                         >  1:305791/1‑134 (MQ=255)
               aTCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGTGTCGTCCCGTACGAt                                                                                                                         >  1:310574/1‑134 (MQ=255)
                    cTGCTTCCCCTTTGCAATAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGata                                                                                                                    <  3:152255/134‑1 (MQ=255)
                    cTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCTTACGATGGATATc                                                                                                                  >  4:290671/1‑136 (MQ=255)
                    cTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGatat                                                                                                                   >  6:51498/1‑135 (MQ=255)
                    cTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATc                                                                                                                  >  3:87104/1‑136 (MQ=255)
                     tGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGata                                                                                                                    <  5:129926/133‑1 (MQ=255)
                     tGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGata                                                                                                                    <  8:265298/133‑1 (MQ=255)
                          ccACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATc                                                                                                            >  3:276709/1‑136 (MQ=255)
                           cACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATc                                                                                                            <  4:87104/135‑1 (MQ=255)
                            aCTTTGCAAGAACC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCgg                                                                                                          <  4:350953/136‑1 (MQ=255)
                             cTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCgg                                                                                                          <  7:195571/135‑1 (MQ=255)
                             cTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCgg                                                                                                          >  7:75824/1‑135 (MQ=255)
                             cTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCgg                                                                                                          <  8:52652/135‑1 (MQ=255)
                             cTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCgg                                                                                                          <  5:73670/135‑1 (MQ=255)
                                 gCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATgg                                                                                                      >  5:110010/1‑135 (MQ=255)
                                  cAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCAACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCGCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATggg                                                                                                     <  1:220672/135‑1 (MQ=255)
                                   aaGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATggg                                                                                                     <  2:231496/134‑1 (MQ=255)
                                        aagtactcCGAGTATTGAAAATTGGCTCCTTGAGATACCGGGGTTTAGCCCAAGCTTGCGAACAAAACTCGCTAACGGCACGGCTCCGGTATAGCTTCGTCCCGTACGATGGATATCCCAATCGGATGTTGTTca                                                                                                <  6:56344/130‑1 (MQ=12)
                                         c‑TTCTCCCAGTCATCCAAATAGGCGCCTTCTGCTACGCGGTTTGAGCCAAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTcagc                                                                                              <  5:132914/135‑1 (MQ=21)
                                         c‑TTCCCCCAGTCATGCAAATCGGCGCCTTCCGCTCCCGGGGTGGAGCCCAAGCTGCCCCAAACAAACCGCGCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTcagc                                                                                              <  5:272796/135‑1 (MQ=21)
                                         c‑TTCACCCAGCCATCAAAATCGGCGCCTTCCGCTACCCGGGTGGCCCCAATGCTTGCCCACACAAACCGCCCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTcagc                                                                                              <  1:354766/135‑1 (MQ=18)
                                              tcCCAGTCATGCAAATAGGCGCCTTCATCTACCCCGGTTGAGCCCAAGCTTCCGCACACAAAACGATCACGCCACGGCTCCGGTAGAGCGTCGTCCCGTCCGATGGATATCCCAATCGGATGTGGTTCAGCAGtaa                                                                                         >  4:271658/1‑134 (MQ=21)
                                                ccAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGCGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGcgtc                                                                                                                                     <  6:246729/90‑1 (MQ=255)
                                                ccAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAACCGCTCACGCCACGGCTCCGGTATAGcgtc                                                                                                                                     >  5:246729/1‑90 (MQ=255)
                                                      aTGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATAc                                                                                 <  3:254770/136‑1 (MQ=255)
                                                      aTGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCGCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATAc                                                                                 <  3:114339/136‑1 (MQ=255)
                                                         cAAATCGGCGCCTTCCGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATAcgc                                                                               <  3:134196/135‑1 (MQ=255)
                                                          aaaTAGGCGCCTTAAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATAcgc                                                                               <  8:53207/134‑1 (MQ=255)
                                                                gcgcCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCtcat                                                                       <  3:163111/136‑1 (MQ=255)
                                                                   ccTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCtcatca                                                                     >  2:267367/1‑135 (MQ=255)
                                                                      tCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACgg                                                                 >  3:93149/1‑136 (MQ=255)
                                                                        aGCTACCCGGGTTGAGTCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACg                                                                  <  5:358919/133‑1 (MQ=255)
                                                                         gCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGt                                                                                                                              <  6:267979/72‑1 (MQ=255)
                                                                         gCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGt                                                                                                                              >  5:267979/1‑72 (MQ=255)
                                                                          cTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACggtggt                                                             <  3:290671/136‑1 (MQ=255)
                                                                             cccGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGa                                                           >  6:180905/1‑135 (MQ=255)
                                                                             cccGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGAt                                                          >  3:68525/1‑136 (MQ=255)
                                                                                gggTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGcgtc                                                                                                                                     >  3:269088/1‑58 (MQ=255)
                                                                                gggTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGcgtc                                                                                                                                     <  4:269088/58‑1 (MQ=255)
                                                                                gggTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATcc                                                        >  5:1330/1‑135 (MQ=255)
                                                                                gggTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGCTATAGCGTCGTCCCGTACGATGGATATcc                                                                                                                 >  7:34122/1‑78 (MQ=255)
                                                                                gggTTGAGCCCAAGCTTGCGCACAAAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATcc                                                                                                                 <  8:34122/78‑1 (MQ=255)
                                                                                 ggTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCACAATCGGATGGGGTTAAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCg                                                       >  2:313795/1‑135 (MQ=255)
                                                                                     gAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGAcc                                                    >  8:267639/1‑134 (MQ=255)
                                                                                      cgCCCAAGCTGGCCCACACAACACACCCCCGCCACGGCTCCGGTATAGCGGCGTCCCGTACGATGGATATCCCACTCGGATGGGGGTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTg                                                  <  2:327986/134‑1 (MQ=18)
                                                                                       gCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGCTCCGACCTgc                                                 >  5:216780/1‑134 (MQ=255)
                                                                                        cccAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCa                                                                                         <  6:316801/94‑1 (MQ=255)
                                                                                        cccAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCa                                                                                         >  5:316801/1‑94 (MQ=255)
                                                                                           aaGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACcca                                             <  5:180905/135‑1 (MQ=255)
                                                                                                    cacacaACACGCTCACGCCACGGCTCCGGTATAGCGTCGGCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCt                                    <  7:595/135‑1 (MQ=255)
                                                                                                     acacaAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCtt                                   >  5:89079/1‑135 (MQ=255)
                                                                                                         aaaaCGCTCACGCCTCGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTc                               <  8:181451/135‑1 (MQ=255)
                                                                                                          aaaCGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCg                                                       >  1:243173/1‑110 (MQ=255)
                                                                                                          aaaCGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCg                                                       <  2:243173/110‑1 (MQ=255)
                                                                                                              gCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCctgct                          <  7:266789/135‑1 (MQ=255)
                                                                                                                   cGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCa                     <  8:75824/135‑1 (MQ=255)
                                                                                                                    gCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATCCGCGATCTCATCAACGGTGGTGATCCGACCTTACCCAAGCCAAGCTTGTTCCTGCTGGTCaa                    >  2:98538/1‑135 (MQ=255)
                                                                                                                    gCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCaa                    >  2:185277/1‑135 (MQ=255)
                                                                                                                      cACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCctgct                          >  4:282485/1‑127 (MQ=255)
                                                                                                                      cACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCctgct                          >  4:277393/1‑127 (MQ=255)
                                                                                                                      cACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCctgct                          <  3:282485/127‑1 (MQ=255)
                                                                                                                      cACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCctgct                          <  3:277393/127‑1 (MQ=255)
                                                                                                                            tCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATcc            <  7:119258/135‑1 (MQ=255)
                                                                                                                               ggTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAg          <  6:71873/134‑1 (MQ=255)
                                                                                                                               ggTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAg          <  2:121605/134‑1 (MQ=255)
                                                                                                                                  ataGTGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAGCAcc      <  2:310574/135‑1 (MQ=255)
                                                                                                                                  ataGTGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAGCAcc      <  2:305791/135‑1 (MQ=255)
                                                                                                                                   taGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAGCACCCt    <  4:309550/136‑1 (MQ=255)
                                                                                                                                    aGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAGCACCCt    <  6:89079/135‑1 (MQ=255)
                                                                                                                                     gCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAGCACCCTc   <  4:185618/135‑1 (MQ=255)
                                                                                                                                      cgtcgtCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAGCACCCTCa  <  7:267639/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CGTTCAAGGTGAGCCATCTCCTGCTTCCACTTTGCAAGAAAC‑TTCTCCCAGTCATGCAAATAGGCGCCTTCAGCTACCCGGGTTGAGCCCAAGCTTGCGCACACAAAACGCTCACGCCACGGCTCCGGTATAGCGTCGTCCCGTACGATGGATATCCCAATCGGATGGGGTTCAGCAGTCACCTGATACGCGATCTCATCAACGGTGGTGATCCGACCTGACCCAAGCCAAGCTTGTTCCTGCTGGTCAATCTCATCCAGCACCCTCA  >  KT2440/4187827‑4188094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑