Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,213,235 G→A 8.9% intergenic (‑279/‑94) CJ019_03953 ← / → CJ019_03954 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,213,2350GA 8.9% 235.8 / 11.5 79intergenic (‑279/‑94)CJ019_03953/CJ019_03954hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (43/29);  new base A (5/2);  total (48/31)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

CATCCAGGGCAAAACCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTCGCTTTGAGTGCC  >  KT2440/4213100‑4213367
                                                                                                                                       |                                                                                                                                    
catccaGGGCAAAACCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATg                                                                                                                                      >  4:328823/1‑136 (MQ=255)
catccaGGGCAAAACCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATg                                                                                                                                      >  4:320638/1‑136 (MQ=255)
     aGGGCAAAACCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCaa                                                                                                                                  >  5:20690/1‑135 (MQ=255)
        gCAAAACCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAAtt                                                                                                                               >  6:368065/1‑135 (MQ=255)
        gCAAAACCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAAtt                                                                                                                               >  6:258278/1‑135 (MQ=255)
          aaaaCCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTgg                                                                                                                             <  1:231127/135‑1 (MQ=255)
          aaaaCCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTg                                                                                                                              >  7:217723/1‑134 (MQ=255)
          aaaaCCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTg                                                                                                                              >  8:187968/1‑134 (MQ=255)
          aaaaCCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTg                                                                                                                              >  2:231127/1‑134 (MQ=255)
          aaaaCCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATAGCAAATTg                                                                                                                              >  1:88048/1‑134 (MQ=255)
               cTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCa                                                                                                                        >  1:10472/1‑135 (MQ=255)
               cTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCa                                                                                                                        >  8:125665/1‑135 (MQ=255)
               cTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCAc                                                                                                                       >  3:157543/1‑136 (MQ=255)
                  ggAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACt                                                                                                                      <  3:255084/134‑1 (MQ=255)
                      tCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAg                                                                                                                 <  3:60749/135‑1 (MQ=255)
                      tCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAg                                                                                                                 <  8:301577/135‑1 (MQ=255)
                       cTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGt                                                                                                                <  1:156491/135‑1 (MQ=255)
                          acaaacaaGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCttt                                                                                                            <  3:320638/136‑1 (MQ=255)
                           caaacaaGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTc                                                                                                               <  7:198903/132‑1 (MQ=255)
                           caaacaaGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCtt                                                                                                             >  2:8930/1‑134 (MQ=255)
                           caaacaaGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCtt                                                                                                             >  7:249928/1‑134 (MQ=255)
                           caaacaaGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATATGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCtgt                                                                                                            >  5:296043/1‑133 (MQ=255)
                                     gCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAAt                                                                                                  <  6:224643/135‑1 (MQ=255)
                                     gCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAAt                                                                                                  <  8:268089/135‑1 (MQ=255)
                                     gCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACATACAAt                                                                                                  >  2:277033/1‑135 (MQ=255)
                                             tAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGCCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGct                                                                                          >  1:238122/1‑135 (MQ=255)
                                                      tGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCt                                                                                  >  8:188327/1‑134 (MQ=255)
                                                        gTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCttt                                                                                >  6:204871/1‑134 (MQ=255)
                                                             gCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATc                                                                          <  6:20690/135‑1 (MQ=255)
                                                             gCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATc                                                                          <  5:310024/135‑1 (MQ=255)
                                                                   ggTAGGTCAGTATACCGCTGGTCAGGATTCCTCCATATTCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGGAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAgg                                                                    <  2:238122/135‑1 (MQ=255)
                                                                    gTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAgg                                                                    <  7:309426/134‑1 (MQ=255)
                                                                     tAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCCCCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTg                                                                  >  8:360525/1‑135 (MQ=255)
                                                                        cTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCAAACCATGGAAAATTGGAATCACTGAACGTATTTCACATACAATGCGCGGCGCTCACGCTTTAAAATCGGCAGGTGCCg                                                               <  5:204871/135‑1 (MQ=255)
                                                                          aaGAATACCGCTTTTAAGGATTCCTCAATAATGTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGAAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGt                                                             <  2:155720/134‑1 (MQ=255)
                                                                           aGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGt                                                             <  3:334608/134‑1 (MQ=255)
                                                                           aGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTa                                                            >  1:235229/1‑135 (MQ=255)
                                                                           aGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTa                                                            >  5:7045/1‑135 (MQ=255)
                                                                            gAATACCGGTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTACCCCACGCATGGAAAATTGTAATCACTGCAAGTCTTTGACCTACAATGCGCGTCTCTCACGCTTTAAAATCGGCAGGTGCCGGt                                                             <  8:249797/133‑1 (MQ=255)
                                                                            gAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGt                                                             <  2:10472/133‑1 (MQ=255)
                                                                            gAATACCGCTTGTAAGGATTCCTCACTAATCTGCGGTATCAGCCCACTCACACAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTAc                                                           <  6:142626/135‑1 (MQ=255)
                                                                                aCCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTgg                                                       >  1:241546/1‑135 (MQ=255)
                                                                                aCCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTgg                                                       >  6:12966/1‑135 (MQ=255)
                                                                                aCCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGa                                                      >  4:53771/1‑136 (MQ=255)
                                                                                     ttGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATaa                                                   >  1:58844/1‑134 (MQ=255)
                                                                                         ctgtaTTCCTCAATAATCTGGGGTATCAGCCCACTCACCCAAGCATAGCAATTTGGATTCACTGCAAGTGTTTGACCTACAATCCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGgaga                                              <  2:370712/131‑1 (MQ=255)
                                                                                          aGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGAtt                                            >  4:185362/1‑136 (MQ=255)
                                                                                          aGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGAtt                                            >  4:216581/1‑136 (MQ=255)
                                                                                          aGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGAt                                             >  6:132393/1‑135 (MQ=255)
                                                                                          aGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGAt                                             >  6:364253/1‑135 (MQ=255)
                                                                                           ggATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGAtt                                            <  8:48823/135‑1 (MQ=255)
                                                                                           ggATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCGCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGAtt                                            <  8:249928/135‑1 (MQ=255)
                                                                                              ttCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTg                                         <  5:364253/135‑1 (MQ=255)
                                                                                               tcctccATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGgt                                       >  3:42081/1‑136 (MQ=255)
                                                                                                 ctcaATAATCTGCGGTATCAGCCCACTCACACAAGCATGGCAAATTGGAATAACTGCAAGTCTTTTACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCATGTACGTGGATAAGGAGATTttgttg                                      >  1:239838/1‑135 (MQ=255)
                                                                                                    cATAATCTGCGGTATCATCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTa                                   >  7:174151/1‑135 (MQ=255)
                                                                                                    cATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTa                                   >  2:7959/1‑135 (MQ=255)
                                                                                                              ggTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGccc                         >  8:363573/1‑135 (MQ=255)
                                                                                                              ggTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCccg                        >  3:341687/1‑136 (MQ=255)
                                                                                                              ggTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCccg                        >  4:121109/1‑136 (MQ=255)
                                                                                                              ggTATCAGCCCACTCACCCAAGCATAGCAAATTGGAATCACTGCAAGTCTTTGACCTACAAt                                                                                                  >  8:106081/1‑62 (MQ=255)
                                                                                                              ggTATCAGCCCACTCACCCAAGCATAGCAAATTGGAATCACTGCAAGTCTTTGACCTACAAt                                                                                                  <  7:106081/62‑1 (MQ=255)
                                                                                                              ggTATCAGCCCACTCACCCAAGCATAGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATTCGCGGCTCTCAAGCTTTAAAATCGTCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCccg                        >  4:124716/1‑136 (MQ=255)
                                                                                                              ggTATCAGCCCACTCACCCAAGCATAGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGcac                         >  1:114364/1‑133 (MQ=255)
                                                                                                              ggTATCAGCCCACTCACCCAAGCATAGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGAGGATAAGGAGATTTTGGTGTTATTGAATGCccg                        >  3:136071/1‑136 (MQ=255)
                                                                                                               gTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCccg                        <  5:258278/135‑1 (MQ=255)
                                                                                                                tATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCAGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCccgc                       >  8:358104/1‑135 (MQ=255)
                                                                                                                 aTCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGAGAAGGAGATTTTGGTGTTATTGAATGCccgcc                      >  7:10716/1‑135 (MQ=255)
                                                                                                                      cccACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCttt                 <  8:182846/135‑1 (MQ=255)
                                                                                                                        cACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCttttt               >  6:67976/1‑135 (MQ=255)
                                                                                                                             aCCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATc                                                                          <  4:275713/71‑1 (MQ=255)
                                                                                                                             aCCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATc                                                                          >  3:275713/1‑71 (MQ=255)
                                                                                                                             aCCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGgaga                                              >  8:33818/1‑99 (MQ=255)
                                                                                                                             aCCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGgaga                                              <  7:33818/99‑1 (MQ=255)
                                                                                                                             aCCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTCGCttt         >  4:85583/1‑136 (MQ=255)
                                                                                                                             aCCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTAGCtt          >  1:367103/1‑135 (MQ=255)
                                                                                                                               ccAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTCGCtt          >  5:363930/1‑133 (MQ=255)
                                                                                                                                    cATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTCGCTTTGAGTGc   <  5:368065/135‑1 (MQ=255)
                                                                                                                                    cATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTCGCTTTGAGTGc   <  7:187968/135‑1 (MQ=255)
                                                                                                                                    cATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTCGCTTTGAGTGc   <  2:145436/135‑1 (MQ=255)
                                                                                                                                     aTGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTCGCTTTGAGTGcc  >  6:217782/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CATCCAGGGCAAAACCTTGGAGTCTGACAAACAAGGTGCCCGTTCTAGACGTATTGGTGAGGCACTGGGTAGCTCAGAATACCGCTTGTCAGGATTCCTCCATAATCTGCGGTATCAGCCCACTCACCCAAGCATGGCAAATTGGAATCACTGCAAGTCTTTGACCTACAATGCGCGGCTCTCACGCTTTAAAATCGGCAGGTGCCGGTACGTGGATAAGGAGATTTTGGTGTTATTGAATGCCCGCCGCTTTTTCGCTTTGAGTGCC  >  KT2440/4213100‑4213367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: