Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,245,134	A→C	29.0%	L182V (TTG→GTG)	CJ019_03982 ←	Crotonyl‑CoA hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,245,134	0	A	C	29.0%	59.6 / 9.2	64	L182V (TTG→GTG)	CJ019_03982	Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (33/11);  new base C (2/18);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.25e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245001‑4245265                                                                                                                                      |                                                                                                                                    aGGGTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCAAc                                                                                                                                    >  2:124977/1‑135 (MQ=255)  gggTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAAcc                                                                                                                                   <  7:101803/135‑1 (MQ=255)      gcgcCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCCCCCcggc                                                                                                                               >  6:191255/1‑135 (MQ=255)       cgcCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCcggcg                                                                                                                              >  8:313704/1‑135 (MQ=255)          cGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGGTCGGCGGCAACGGCCGGCCACTGCTCGTCGGCGCCGCCGTCGCTGACCAACCCGGCGGccc                                                                                                                          >  3:47132/1‑135 (MQ=255)            ggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCCACCCGGCGGCCCgg                                                                                                                        >  4:88145/1‑136 (MQ=255)                ccGTCGCGTATCCGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCCACTGCTCGTCGGCGCAGCCTTCGCTGGCCCACCCGGCGGCCCGGgcg                                                                                                                     >  8:234357/1‑135 (MQ=255)                ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGGTCGGCGGCACCGGCAGGCAACTGCTCGTCGGCGCCGCCTTCGCGGCCCAACCCGGCGGCCCggggg                                                                                                                     >  5:145084/1‑133 (MQ=255)                                gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCCGCCCGGGCGCGAACCGCCCCCcacc                                                                                                     >  2:196564/1‑134 (MQ=255)                                    ggTCTGCGCATAGGCGAAGGTCGGGCCATTTGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCCCGCACGGCGCCCcccaccac                                                                                                 >  8:82362/1‑131 (MQ=255)                                    ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACtc                                                                                                 >  8:263012/1‑135 (MQ=255)                                    ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAAcgcg                                                                                                >  4:89894/1‑136 (MQ=255)                                    ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACCACtgc                                                                                                >  3:272385/1‑134 (MQ=255)                                        tGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGGGCGCTCCGCGGCCCCCCACacgtcg                                                                                             >  7:176086/1‑135 (MQ=255)                                          cgcATCGGCGATGGTCGGGCCATTGGCCAGGCGCCGGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAg                                                                                           <  8:176086/135‑1 (MQ=255)                                           gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACGTCGCTGACCAACCCGGCGGCCACGGCGCGATCGGCGCCCCCCCACTCGTCGAg                                                                                           >  2:244592/1‑134 (MQ=255)                                               cggCGACGGGCGGGCCATGGGCCCGGCGCCCGGCCAGTGCGGGGGCAACGGCAGGAAACTGCTCGTCGGCGCAGACTTCGCTCACCACCCCGGGGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  6:153150/134‑1 (MQ=255)                                               cggCGACGGGCGGGCCATGGGCCCGGCGCCCGGCCAGTGCGGCGGCACCGGCCGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  2:80131/134‑1 (MQ=255)                                                  cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACGTCGCTGACCAACCCGGCGGCCCCGGCGCGCTCCGCGCCCCCCCACACCTGGCGGAAaagca                                                                                   >  6:304105/1‑135 (MQ=255)                                                   gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACCACTCGTCGAGGCAccgccg                                                                                  >  2:130705/1‑133 (MQ=255)                                                   gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCCGTCGGGGCCCCACCACACCGCGAGGCAcaccacg                                                                                 >  3:327042/1‑134 (MQ=255)                                                    atggTGGGGCCTTTGGCCAGGCGCCCGGCCTGGCGGGGGGCAAGGGCAGTAACCTCGTCGGCGGCTCAGACTGGGCTGACAACCCCGGCGCCCCGGGCGGTATCGCCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                 <  6:307437/133‑1 (MQ=255)                                                    atggTCGGGCCATTGGCCAGGCGCCCGGCAAGTTGGGCGGCAACGGCAGGCAACTGCTGGTCGGGGCTGACTTCGCTCACCAACCCGGCGGCCAGGGCGCGATGGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                 <  8:267626/133‑1 (MQ=255)                                                       gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACCACTCGTCGAGGCACAGCAAgcgc                                                                              >  6:339609/1‑135 (MQ=255)                                                       gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACGCGTCGCGGCACAGCACgcgcg                                                                             >  3:165537/1‑135 (MQ=255)                                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGCACAGCAGGCGCGTGGCCt                                                                       >  6:97286/1‑135 (MQ=255)                                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGGACAGCAGGCGCTTGGCCt                                                                       >  6:196809/1‑135 (MQ=255)                                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGCGCGGCGCCCCCCAACACGGCGCGGCACACCAGGCGCGTgggccg                                                                      >  4:337145/1‑132 (MQ=255)                                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCCCTAGGCGCCCCCCCACACCTCGCGGAACAGCCGGCGCTTGGccg                                                                       >  5:176027/1‑134 (MQ=255)                                                                  gcccgggCGCCCGGCCAGTGCGGCGCAAAGGGCAGGAATCTGCGGGGGGGCGACCACTGCGCTGCCCACCCCGCCGGCCTGGGCGCTATCGCCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                  <  4:349966/131‑1 (MQ=255)                                                                   gCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGGCGAGGCACAGCAGGCGCTTGGCCTGCTCg                                                                  >  8:213734/1‑135 (MQ=255)                                                                    ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAAcc                                                                                                                                   >  5:293616/1‑69 (MQ=255)                                                                    ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAAcc                                                                                                                                   <  6:293616/69‑1 (MQ=255)                                                                    ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACCACTCGTCGAGGCACAGCAGGCGCTTGGGCTGCTCGc                                                                 >  8:103932/1‑135 (MQ=255)                                                                       ggCGCCGGGCCAGGGCGGCGGCAAGGGCAGGCAACGGGGCGGCGGCGCCGACTGCGCTGCCCAACCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  4:227270/136‑1 (MQ=255)                                                                       ggCGCCCGGCCTGTTCGGCGGCACCGCCAGCCAACTGCGCGGCGGCGCAGACTGCGCTCCCCACCCCGGCGCCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGc                                                              <  2:70380/135‑1 (MQ=255)                                                                       ggCGCCCGGCCTGTTCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTGCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  3:84815/136‑1 (MQ=255)                                                                        gcgcCCGGCCTGTGCGGCGCCACCGCCCGCCAAGGGCGCGGCGGCCCAGACTGCGCTCCCCACCCGGGCGGCCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  2:340185/135‑1 (MQ=255)                                                                         cgcCCGGACAGGGCGGCGGCCACGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCg                                                            <  8:256997/135‑1 (MQ=255)                                                                           cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACCGCCGGCGCTTGGCCTGCTCGCTGCCGAt                                                          >  4:111092/1‑135 (MQ=255)                                                                                ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGcc                                                     >  2:33987/1‑135 (MQ=255)                                                                                  tgtgcggcggCAACGGCAGGCAACGGCGCGGCGGCGCAGACTGCGCTGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  8:365325/131‑1 (MQ=255)                                                                                       ggcggcAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCGCGGCGCCCCCCCACTCGTCGCGGCACAGCAGGCGCGTGGCCTGCGCGCTGCCGGTCAGCCGGGGccc                                              >  1:66326/1‑133 (MQ=255)                                                                                                  caggcaACTGCGCGGCGGCGCAGACTTCGCGGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                  <  4:165537/136‑1 (MQ=255)                                                                                                   tgccacctgctgGTCGGCCCTGAGTTCGCTGCCCAACCCGGGGGCCAGGGGGGGTGGGCCGCCCCACAACTCGTCGAGGAACAGCTGGGGGTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                  <  8:55358/129‑1 (MQ=255)                                                                                                      caACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGGCGCGGAACAGCCCGCGCTTGGCCTGCTCGCTGCCGCTCAGCCGGGGCAGGTGCCCGCTGGCGCCg                              >  4:74247/1‑136 (MQ=255)                                                                                                         cTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACGCGGCGCGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGCTCCGCCGTGGCAGGTGCCAGCTGGCGCCGGc                            >  8:255579/1‑135 (MQ=255)                                                                                                         cTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGGGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  4:175497/136‑1 (MQ=255)                                                                                                          tGCGCGGCGGCGCAGACTGCGCGGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  8:20587/135‑1 (MQ=255)                                                                                                             tcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCTGGCGATCCGCCGTGGCAGGTGCCCGCTGGCGCCGGCATCg                        >  6:364047/1‑135 (MQ=255)                                                                                                             tcggcgGCGCAGACTGCGCTGACCACCCCGGCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCAt                          <  5:196809/133‑1 (MQ=255)                                                                                                              cgtcgGCGCAGACTGCGCTGACCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  3:89894/136‑1 (MQ=255)                                                                                                               ttcgGCGCATACATGGCTCCCAACCCCGGCGGCCCGGGCGCGAGCGGCGCACCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  3:161565/135‑1 (MQ=255)                                                                                                                gcgGCGCTCACTTCGCGGCCCAACCGGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  1:256855/134‑1 (MQ=255)                                                                                                                gcgGCGCAGACTGCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  7:313704/134‑1 (MQ=255)                                                                                                                gcgGCGCAGACCTCGCTCCCACCCCCGGCGCCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                     <  1:289713/134‑1 (MQ=255)                                                                                                                 cgGCGCTGAGTTCGCGGCCCAACCCGGCGGCCCGGGCGCGAGGGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                   <  4:327042/136‑1 (MQ=255)                                                                                                                       aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGGACAGCAGGCGCGTGGCCTGCTCGCTGCCGCTCAGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCGGGGCGGTAGcc             >  3:98900/1‑136 (MQ=255)                                                                                                                       aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTAGGCGGCCCCCCACTCGGCGAGGAACACCCCGCGCGTTGCCAGCTCGCTGCCGCTCAGCCGGGGCAGGTGGCACCTGGCGCCCGCCTCGGGGCGGTatc              >  6:291401/1‑133 (MQ=255)                                                                                                                         aCTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCa           <  4:98900/136‑1 (MQ=255)                                                                                                                              gCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGCGGCACCGCAGGCCGGTTGCCGGGTCCCAGGCGCTCAGCCGGGGGAGGTGGCAGCGGGCGCCGGCCACGGGGCGGTAGccccagcg       >  6:371144/1‑130 (MQ=255)                                                                                                                                  accaccCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  4:47132/136‑1 (MQ=255)                                                                                                                                  aCCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  4:187436/136‑1 (MQ=255)                                                                                                                                   ccACCCGGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  6:176027/135‑1 (MQ=255)                                                                                                                                   ccACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  8:186297/135‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    AGGGTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245001‑4245265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑