Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,245,145 A→C 62.5% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1450AC62.5% 5.9 / 15.3 59L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (6/14);  new base C (25/11);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 5.83e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

GGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGC  >  KT2440/4245012‑4245272
                                                                                                                                     |                                                                                                                               
ggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCCACCCGGCGGCCCgg                                                                                                                               >  4:88145/1‑136 (MQ=255)
    ccGTCGCGTATCCGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCCACTGCTCGTCGGCGCAGCCTTCGCTGGCCCACCCGGCGGCCCGGgcg                                                                                                                            >  8:234357/1‑135 (MQ=255)
    ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGGTCGGCGGCACCGGCAGGCAACTGCTCGTCGGCGCCGCCTTCGCGGCCCAACCCGGCGGCCCggggg                                                                                                                            >  5:145084/1‑133 (MQ=255)
                    gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCCGCCCGGGCGCGAACCGCCCCCcacc                                                                                                            >  2:196564/1‑134 (MQ=255)
                        ggTCTGCGCATAGGCGAAGGTCGGGCCATTTGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCCCGCACGGCGCCCcccaccac                                                                                                        >  8:82362/1‑131 (MQ=255)
                        ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACtc                                                                                                        >  8:263012/1‑135 (MQ=255)
                        ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAAcgcg                                                                                                       >  4:89894/1‑136 (MQ=255)
                        ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACCACtgc                                                                                                       >  3:272385/1‑134 (MQ=255)
                            tGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGGGCGCTCCGCGGCCCCCCACacgtcg                                                                                                    >  7:176086/1‑135 (MQ=255)
                              cgcATCGGCGATGGTCGGGCCATTGGCCAGGCGCCGGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAg                                                                                                  <  8:176086/135‑1 (MQ=255)
                               gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACGTCGCTGACCAACCCGGCGGCCACGGCGCGATCGGCGCCCCCCCACTCGTCGAg                                                                                                  >  2:244592/1‑134 (MQ=255)
                                   cggCGACGGGCGGGCCATGGGCCCGGCGCCCGGCCAGTGCGGGGGCAACGGCAGGAAACTGCTCGTCGGCGCAGACTTCGCTCACCACCCCGGGGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                             <  6:153150/134‑1 (MQ=255)
                                   cggCGACGGGCGGGCCATGGGCCCGGCGCCCGGCCAGTGCGGCGGCACCGGCCGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                             <  2:80131/134‑1 (MQ=255)
                                      cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACGTCGCTGACCAACCCGGCGGCCCCGGCGCGCTCCGCGCCCCCCCACACCTGGCGGAAaagca                                                                                          >  6:304105/1‑135 (MQ=255)
                                       gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACCACTCGTCGAGGCAccgccg                                                                                         >  2:130705/1‑133 (MQ=255)
                                       gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCCGTCGGGGCCCCACCACACCGCGAGGCAcaccacg                                                                                        >  3:327042/1‑134 (MQ=255)
                                        atggTGGGGCCTTTGGCCAGGCGCCCGGCCTGGCGGGGGGCAAGGGCAGTAACCTCGTCGGCGGCTCAGACTGGGCTGACAACCCCGGCGCCCCGGGCGGTATCGCCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                        <  6:307437/133‑1 (MQ=255)
                                        atggTCGGGCCATTGGCCAGGCGCCCGGCAAGTTGGGCGGCAACGGCAGGCAACTGCTGGTCGGGGCTGACTTCGCTCACCAACCCGGCGGCCAGGGCGCGATGGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                        <  8:267626/133‑1 (MQ=255)
                                           gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACCACTCGTCGAGGCACAGCAAgcgc                                                                                     >  6:339609/1‑135 (MQ=255)
                                           gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACGCGTCGCGGCACAGCACgcgcg                                                                                    >  3:165537/1‑135 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGCACAGCAGGCGCGTGGCCt                                                                              >  6:97286/1‑135 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGGACAGCAGGCGCTTGGCCt                                                                              >  6:196809/1‑135 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGCGCGGCGCCCCCCAACACGGCGCGGCACACCAGGCGCGTgggccg                                                                             >  4:337145/1‑132 (MQ=255)
                                                  cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCCCTAGGCGCCCCCCCACACCTCGCGGAACAGCCGGCGCTTGGccg                                                                              >  5:176027/1‑134 (MQ=255)
                                                      gcccgggCGCCCGGCCAGTGCGGCGCAAAGGGCAGGAATCTGCGGGGGGGCGACCACTGCGCTGCCCACCCCGCCGGCCTGGGCGCTATCGCCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                         <  4:349966/131‑1 (MQ=255)
                                                       gCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGGCGAGGCACAGCAGGCGCTTGGCCTGCTCg                                                                         >  8:213734/1‑135 (MQ=255)
                                                        ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACCACTCGTCGAGGCACAGCAGGCGCTTGGGCTGCTCGc                                                                        >  8:103932/1‑135 (MQ=255)
                                                           ggCGCCGGGCCAGGGCGGCGGCAAGGGCAGGCAACGGGGCGGCGGCGCCGACTGCGCTGCCCAACCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                    <  4:227270/136‑1 (MQ=255)
                                                           ggCGCCCGGCCTGTTCGGCGGCACCGCCAGCCAACTGCGCGGCGGCGCAGACTGCGCTCCCCACCCCGGCGCCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGc                                                                     <  2:70380/135‑1 (MQ=255)
                                                           ggCGCCCGGCCTGTTCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTGCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                    <  3:84815/136‑1 (MQ=255)
                                                            gcgcCCGGCCTGTGCGGCGCCACCGCCCGCCAAGGGCGCGGCGGCCCAGACTGCGCTCCCCACCCGGGCGGCCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                    <  2:340185/135‑1 (MQ=255)
                                                             cgcCCGGACAGGGCGGCGGCCACGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCg                                                                   <  8:256997/135‑1 (MQ=255)
                                                               cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACCGCCGGCGCTTGGCCTGCTCGCTGCCGAt                                                                 >  4:111092/1‑135 (MQ=255)
                                                                    ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGcc                                                            >  2:33987/1‑135 (MQ=255)
                                                                      tgtgcggcggCAACGGCAGGCAACGGCGCGGCGGCGCAGACTGCGCTGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                          <  8:365325/131‑1 (MQ=255)
                                                                           ggcggcAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCGCGGCGCCCCCCCACTCGTCGCGGCACAGCAGGCGCGTGGCCTGCGCGCTGCCGGTCAGCCGGGGccc                                                     >  1:66326/1‑133 (MQ=255)
                                                                                      caggcaACTGCGCGGCGGCGCAGACTTCGCGGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                         <  4:165537/136‑1 (MQ=255)
                                                                                       tgccacctgctgGTCGGCCCTGAGTTCGCTGCCCAACCCGGGGGCCAGGGGGGGTGGGCCGCCCCACAACTCGTCGAGGAACAGCTGGGGGTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                         <  8:55358/129‑1 (MQ=255)
                                                                                          caACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGGCGCGGAACAGCCCGCGCTTGGCCTGCTCGCTGCCGCTCAGCCGGGGCAGGTGCCCGCTGGCGCCg                                     >  4:74247/1‑136 (MQ=255)
                                                                                             cTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACGCGGCGCGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGCTCCGCCGTGGCAGGTGCCAGCTGGCGCCGGc                                   >  8:255579/1‑135 (MQ=255)
                                                                                             cTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGGGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                  <  4:175497/136‑1 (MQ=255)
                                                                                              tGCGCGGCGGCGCAGACTGCGCGGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                  <  8:20587/135‑1 (MQ=255)
                                                                                                 tcgtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCTGGCGATCCGCCGTGGCAGGTGCCCGCTGGCGCCGGCATCg                               >  6:364047/1‑135 (MQ=255)
                                                                                                 tcggcgGCGCAGACTGCGCTGACCACCCCGGCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCAt                                 <  5:196809/133‑1 (MQ=255)
                                                                                                  cgtcgGCGCAGACTGCGCTGACCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                             <  3:89894/136‑1 (MQ=255)
                                                                                                   ttcgGCGCATACATGGCTCCCAACCCCGGCGGCCCGGGCGCGAGCGGCGCACCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                            <  3:161565/135‑1 (MQ=255)
                                                                                                    gcgGCGCTCACTTCGCGGCCCAACCGGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                            <  1:256855/134‑1 (MQ=255)
                                                                                                    gcgGCGCAGACTGCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                            <  7:313704/134‑1 (MQ=255)
                                                                                                    gcgGCGCAGACCTCGCTCCCACCCCCGGCGCCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                            <  1:289713/134‑1 (MQ=255)
                                                                                                     cgGCGCTGAGTTCGCGGCCCAACCCGGCGGCCCGGGCGCGAGGGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                          <  4:327042/136‑1 (MQ=255)
                                                                                                           aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGGACAGCAGGCGCGTGGCCTGCTCGCTGCCGCTCAGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCGGGGCGGTAGcc                    >  3:98900/1‑136 (MQ=255)
                                                                                                           aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTAGGCGGCCCCCCACTCGGCGAGGAACACCCCGCGCGTTGCCAGCTCGCTGCCGCTCAGCCGGGGCAGGTGGCACCTGGCGCCCGCCTCGGGGCGGTatc                     >  6:291401/1‑133 (MQ=255)
                                                                                                             aCTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCa                  <  4:98900/136‑1 (MQ=255)
                                                                                                                  gCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGCGGCACCGCAGGCCGGTTGCCGGGTCCCAGGCGCTCAGCCGGGGGAGGTGGCAGCGGGCGCCGGCCACGGGGCGGTAGccccagcg              >  6:371144/1‑130 (MQ=255)
                                                                                                                      accaccCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt         <  4:47132/136‑1 (MQ=255)
                                                                                                                      aCCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt         <  4:187436/136‑1 (MQ=255)
                                                                                                                       ccACCCGGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt         <  6:176027/135‑1 (MQ=255)
                                                                                                                       ccACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt         <  8:186297/135‑1 (MQ=255)
                                                                                                                              ggcggcCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCCGGGGGGTGTAGCCGGc  >  7:34825/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                               
GGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGC  >  KT2440/4245012‑4245272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑