Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,291,840 T→G 30.4% intergenic (‑142/‑20) sdhL ← / → CJ019_04025 Shikimate dehydrogenase‑like protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,291,8400TG30.4% 67.1 / 7.0 69intergenic (‑142/‑20)sdhL/CJ019_04025Shikimate dehydrogenase‑like protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (16/30);  new base G (21/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 7.10e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACTGAAACGTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCGGG  >  KT2440/4291716‑4291975
                                                                                                                             |                                                                                                                                       
tGACTGAAACGTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCg                                                                                                                                <  7:211740/134‑1 (MQ=255)
 gACTGAAACGTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAg                                                                                                                              <  7:151650/135‑1 (MQ=255)
 gACTGAAACGTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTGCGGCGAg                                                                                                                              >  5:61807/1‑135 (MQ=255)
   cTGAAACGTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTACTGCGAGgt                                                                                                                            >  8:313757/1‑135 (MQ=255)
     gAAACGTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTTCGGGGGGgttg                                                                                                                          >  5:366632/1‑135 (MQ=255)
     gAAACGTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTGCTGCGAGggtgt                                                                                                                         >  4:132111/1‑136 (MQ=255)
          gTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTAt                                                                                                                    <  3:292147/136‑1 (MQ=255)
          gTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTAt                                                                                                                    <  4:342076/136‑1 (MQ=255)
                       aaCAGAGTCAGCCACTGGTCGTCCCTTACACCACCATTACCGTTCCCCTGTCCTCAGCTGACAGCGGCCGGAGTCATAGTAACAGGCCCCCCGACGTGGGGTGTTCCGTCGTGGTTGTTTTATGGACCgggacat                                                                                                        >  7:197491/1‑129 (MQ=255)
                       aaCAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCAt                                                                                                        >  3:307582/1‑135 (MQ=255)
                       aaCAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCAt                                                                                                        <  7:220588/135‑1 (MQ=255)
                        aCAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTTCTGCGAGGTTGTTTTATGAACCCGCTCAtt                                                                                                       >  7:36177/1‑135 (MQ=255)
                            agTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCgg                                                                                                  >  4:164314/1‑136 (MQ=255)
                               cAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGACCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTACTGCGTGGTTGTTTTATGAACCGTCTCATTCCCCGaaa                                                                                               >  4:159153/1‑135 (MQ=255)
                                 tccAATGGTAGTCCCTTACAAAACATTAACCTTTCCCATGTGATCAGCTGACAGAGTCCGGAGTAATAGTAACAGTGCCCGGGACGTGGGGTTTTCCTTCTAGGTTGTTTTATGAACCGTCTCATTCCCTGAATc                                                                                              <  2:132005/134‑1 (MQ=255)
                                 gCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTGTTTCTGCGCGGGTGTTTTTTTCAACGTCTGGTTCCCgcacag                                                                                              >  2:47206/1‑130 (MQ=255)
                                 gCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTGCTGCGGGGTTGTTTTTTTTACCGTCGGATTACCgggaacg                                                                                             >  3:20056/1‑131 (MQ=255)
                                   cACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTGCTGGGCGGTTGTTTTATGAAACGTATCATTACCGGAATCGtg                                                                                           >  4:336618/1‑135 (MQ=255)
                                    aCTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTc                                                                                           <  5:131908/135‑1 (MQ=255)
                                    aCTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTACATCGAGGGTGTTTTATTAAACCTCTCAATACCGGAAtactcc                                                                                          >  3:357272/1‑131 (MQ=255)
                                    aCTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGGGTGCTGCGGGGGTGTTTTTTGAACCCGCGCCTTCCCGGCATCggc                                                                                           >  1:216767/1‑133 (MQ=255)
                                    aCTGGTCGTACCTTACACAACCTTTACCTTTCCCCTGTGCTCAGCTGACAGAGGCAGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTAGCTGCGAGGTTGTTTTATGATCCGTCTCATTCCCGGAATCGTc                                                                                           <  2:58571/135‑1 (MQ=255)
                                           gtcCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTCCAGGGAGGGTGTTTTATGAAACCGCTCAGTACCGGCATCGGCCGGCTgg                                                                                    >  7:311370/1‑135 (MQ=255)
                                                ttACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGt                                                                               <  1:160568/135‑1 (MQ=255)
                                                    acaACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTACTGCGAGGGTGGTTTAATAACCCGCTCAATTCCCGCATCGTCCTGCGGGTCGGTGccc                                                                           >  7:21688/1‑135 (MQ=255)
                                                     caACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTg                                                                         <  4:179814/136‑1 (MQ=255)
                                                      aaCCTTTACCGTTCCCCTGTGCTCCGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGGGGGGTTTTCCTGCGAGGTGGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGGCGGTGCCCTg                                                                         <  5:203348/135‑1 (MQ=255)
                                                         cTTTACCGTTCCCCTGTGATCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTTATGCGAGGTTGTTTTATGAAACGTCAGATTACCCGCATCGTACATCTGGTGGGTGCCCAGGcc                                                                      >  4:110575/1‑135 (MQ=255)
                                                           ttACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTGGTGCGAGGTTGTTTTTTGTTCCGTCGAATTACCGGAATCGTCCTGCTGGTGGGTGGCCTGGCCa                                                                     >  2:194097/1‑134 (MQ=255)
                                                           ttACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGGTTGCTGGGAGGTTGGGTTATTAACCGTCGCATTCCCCGAATCGTCCGGCGGGTGGGTGCCCGGGccga                                                                    >  7:352823/1‑133 (MQ=255)
                                                             aCCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATc                                                                   <  7:267036/134‑1 (MQ=255)
                                                                 ttCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCgg                                                              <  8:72166/135‑1 (MQ=255)
                                                                   ccccTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTGAGGCGAGGTTTTTTTTTGTTCAGTCTTAATGGACGAATCGTCAGGGAGGGCGGTGGCCTGGGCATCAGCGGGc                                                            >  2:124063/1‑135 (MQ=255)
                                                                   ccccTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTCCAGCGAGGTTGTTTTATGAAACCGCGCATTCCCCGAATACTCCTGCTGGTAGGTGCCCGGGCCCTCAGCCGGcc                                                           >  4:162171/1‑136 (MQ=255)
                                                                     ccTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGGTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGccg                                                          >  7:22424/1‑134 (MQ=255)
                                                                     ccTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTGGTGCGAGGTTGTTTTTTTAAACGTCGCATTCCCCGACAAGTCCGGCTGGTCGGTGGCCGGGCCCGCAGCggggcg                                                          >  1:367363/1‑132 (MQ=255)
                                                                      cTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGCATCGTCCTTCGGGTCGGTGGCCTGGGCCTAAGCGGGCCTg                                                         >  6:181782/1‑135 (MQ=255)
                                                                      cTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTGCTGCGAGGGTTTTTTTTTAAAAGTGTCATTACCGGCATCCTCCATCGGGTGGGTGGCCAGGCCATCAGCGGGCCGgc                                                        >  4:321864/1‑136 (MQ=255)
                                                                      cTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTCCAGGGAGGTTGTTTTTTTAACCGGGTGAATACCGGCATAGTCCTGCTGGTCGGTGGCCGCGCCCTCAACGGGCCt                                                          >  8:305964/1‑134 (MQ=255)
                                                                         tgCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTgca                                                       <  4:307582/134‑1 (MQ=255)
                                                                                 tGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTTCTGCGAGGTTGTTTTTTTAAACGTCTCATTCCCGGCATCGTACTGCTGGTCGGTGCCCTGGCCCTCACCCGGCCTGCagccgccccc                                              >  1:223821/1‑131 (MQ=255)
                                                                                   aCAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGGTTGCTTGGGGGGTTGTTTATGAAACGTCTCAATACCGGCAACGGGATGAGGGGCGGTGCCCAGGCCCAGACCGGGCCGGCCGCACCGCAcgg                                            >  6:32287/1‑135 (MQ=255)
                                                                                      gagGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTACTGCGAGGTTTTTTTATTAACCGTCTGATTCCCGGTATCGTCCTGGTGGTCGGTGCCCTTGCCATCCGCCGGCCTGCAGCAGCGCAaggcggg                                        >  4:357361/1‑135 (MQ=255)
                                                                                        ggCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTTTTTTATTAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTTCCCGGGCCATCAGCGGGCCGGCAGCCGCGCACGGCGGCt                                       >  8:12131/1‑135 (MQ=255)
                                                                                        ggCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGACTCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCt                                       <  1:321183/135‑1 (MQ=255)
                                                                                             gAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTGCGTCGAGGGTGTTTTATGAACCGTCTCATTGCCGGCATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATggc                                  >  2:290585/1‑135 (MQ=255)
                                                                                             gAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGGTGTTTTTTTAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCCTCAGCGGGCCGGCAGCAGCGCCCCGCGGCTATggc                                  >  2:221376/1‑135 (MQ=255)
                                                                                             gAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTGCTGGGAGGGTGTTTTTTTATCCGTCTCCTTGCCGGCATCGTGCTGCGGGTCGGTGGCCGGGGCCTCAGCGGGCCGGCAGCAGCGCCCGGCGGCGTTggc                                  >  2:290187/1‑135 (MQ=255)
                                                                                             gAGTCATAGTAACAGGGCCCCGGACGTGGGGGTTTGCGGCGAGGTTGTTTTTTGAACCGTCTCATTCCCGGCATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCGGCCGCAGCGCCCGGCGGCGATggc                                  >  7:67407/1‑135 (MQ=255)
                                                                                              gacagtAGGAACAGGGCCCCGGACGGGGGGGTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATggcg                                 <  5:244144/130‑1 (MQ=255)
                                                                                              aGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATggcg                                 <  7:53599/135‑1 (MQ=255)
                                                                                                      tcaCAGGGCCCCGGACGGGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAACAGCGCACGGCGGCTATGGCGGCCATgg                          <  2:160673/132‑1 (MQ=255)
                                                                                                      tAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATgg                          <  1:221376/134‑1 (MQ=255)
                                                                                                      tAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATgg                          <  2:170110/134‑1 (MQ=255)
                                                                                                      tAACAGGGCCCCGGACGTGGGGGTTTGGTGCGCGGGTGTTTTTTTTACCGGCTGATTGCCGGAATCGTCCGGCGGGTGGGGGGCCGGGGCCGCCGCGGGGCGGCCGCAGCCCCCGGCGGCGATGGCGGCCagcgg                         >  2:321183/1‑131 (MQ=255)
                                                                                                           gtgCCGCGGAGGTGGGGTTTTCCTGCGAGGTTGTTTTATTAACCGTCTCATTCCCGGTATCGTCGTGGTGGTCGGTGCCCTGGCCATGAGGGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCAtggt                   <  4:228990/134‑1 (MQ=255)
                                                                                                           gggCCCCGGACGTGTGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCAtgg                    <  1:348883/135‑1 (MQ=255)
                                                                                                           gggCCCCGGACGTGGGGTTTTGCTGCGAGGGTGTTTTATGAACCGTCTCAATGCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCCGGAGCGGGCCTGCCGCAGCGCCCCGCGGCGAGGGCGGCCAGGGGCAtgg                    >  6:333071/1‑135 (MQ=255)
                                                                                                           gggCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCAtgg                    <  5:181782/135‑1 (MQ=255)
                                                                                                           gggCCCCGGACGTGGGGGTTTCCTGCGAGGTTGATTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCAtggt                   <  4:109928/136‑1 (MQ=255)
                                                                                                                 cGGACGTGGGGTTTTCATGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGtggttg              <  6:295173/135‑1 (MQ=255)
                                                                                                                         ggggTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGGCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGc      <  3:162171/136‑1 (MQ=255)
                                                                                                                           ggTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGc      <  1:124063/134‑1 (MQ=255)
                                                                                                                           ggTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCg    <  3:159153/136‑1 (MQ=255)
                                                                                                                           ggTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCg    <  3:172705/136‑1 (MQ=255)
                                                                                                                            gTTTTCCTGCGTGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCTGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACTGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCgg   <  4:107602/136‑1 (MQ=255)
                                                                                                                            gTTTTCCTGCGAGGTTCTTTTATGAACCGTCTCATTCCCTGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCgg   <  3:357361/136‑1 (MQ=255)
                                                                                                                            gTTTTACTGCGAGGTTGTTTTATTAAACCTATAAATACCCGTATCGTGCAGCGGGTCCGTGCACAGGCCATCACCGTGGCTGCCGCAGCCCACCGCGGCGCGGGCGGGGCAGGGCCTGGTGGTGGGTTTGGGGCg    >  6:143802/1‑135 (MQ=255)
                                                                                                                            gTTGTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCATCAGCGCACGGCGGCTATGGCGGCGATGGGCATGGTGGTTGGTATGGGCCg    <  5:30106/135‑1 (MQ=255)
                                                                                                                             ttttCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCgg   <  1:290187/135‑1 (MQ=255)
                                                                                                                             ttttCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCgg   <  2:223821/135‑1 (MQ=255)
                                                                                                                             ttttCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGGCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCgg   <  8:311370/135‑1 (MQ=255)
                                                                                                                             ttttACAGCGAGGTTGTTTTATTAACCGTCTCATTACCGGAATCGTCCAGCTGGTCGGTGCCCTGGCCAGCAGCCGGCCTGCAGCAGCGCACCGCCGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCggg  >  4:134443/1‑136 (MQ=255)
                                                                                                                             |                                                                                                                                       
TGACTGAAACGTTTATTCAGCGTAACAGAGTCAGCCACTGGTCGTCCCTTACACAACCTTTACCGTTCCCCTGTGCTCAGCTGACAGAGGCCGGAGTCATAGTAACAGGGCCCCGGACGTGGGGTTTTCCTGCGAGGTTGTTTTATGAACCGTCTCATTCCCGGAATCGTCCTGCTGGTCGGTGCCCTGGCCATCAGCGGGCCTGCAGCAGCGCACGGCGGCTATGGCGGCCATGGGCATGGTGGTTGGTATGGGCCGGG  >  KT2440/4291716‑4291975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: