Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 362,975 G→C 6.3% intergenic (+27/‑49) CJ019_00336 → / → CJ019_00337 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440362,9750GC 6.3% 201.1 / 5.5 64intergenic (+27/‑49)CJ019_00336/CJ019_00337hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/24);  new base C (2/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

TCACCGAGACCTTGCTCAAACGCGGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCTGTATGT  >  KT2440/362848‑363109
                                                                                                                               |                                                                                                                                      
tCACCGAGACCTTGCTCAACCGCTGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCaa                                                                                                                                 <  5:258476/135‑1 (MQ=255)
         ccTTGCTCAAACGCGGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGc                                                                                                                        >  8:253071/1‑135 (MQ=255)
               taaaaCGCGGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGATAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCcac                                                                                                                  <  6:265423/133‑1 (MQ=255)
               tCAAACGCGGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGCTAGCCCCTGCCAGCAACGCCGGGGCACCca                                                                                                                   >  7:249707/1‑134 (MQ=255)
               tCAAACGCGGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCcac                                                                                                                  <  1:220990/135‑1 (MQ=255)
               tCAAACGCGGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCca                                                                                                                   >  2:105090/1‑134 (MQ=255)
                 aaaCGCGGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTCCCAGCAACGCCGGGGCACCcaca                                                                                                                 >  6:368225/1‑134 (MQ=255)
                      cgGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAAt                                                                                                           <  2:177114/135‑1 (MQ=255)
                           aTTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTAt                                                                                                      >  5:88966/1‑135 (MQ=255)
                                    gcgTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGgagttgag                                                                                             >  1:28109/1‑135 (MQ=255)
                                                      tGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTCCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCg                                                                           <  5:368225/135‑1 (MQ=255)
                                                         gCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGccc                                                                        >  8:120295/1‑135 (MQ=255)
                                                                    gTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCa                                                             <  2:15489/135‑1 (MQ=255)
                                                                      aaaCGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCCGCAACGCCGGGGCACCCACAAAAACTTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCact                                                           >  8:105604/1‑133 (MQ=255)
                                                                      aaaCGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAAt                                                           >  6:13678/1‑135 (MQ=255)
                                                                      aaaCGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAAt                                                           >  5:364920/1‑135 (MQ=255)
                                                                      aaaCGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAAt                                                           >  7:32354/1‑135 (MQ=255)
                                                                      aaaCGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATc                                                          >  4:55816/1‑136 (MQ=255)
                                                                      aaaCGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATc                                                          >  3:334589/1‑136 (MQ=255)
                                                                      aaaCGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGACGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGTCCAAAATCGCCCCGCAATTGCCAAt                                                           >  5:200353/1‑135 (MQ=255)
                                                                          gTCCTCAAGGAGGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGaa                                                       <  1:309778/135‑1 (MQ=255)
                                                                                aaGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCg                                                   >  2:230997/1‑133 (MQ=255)
                                                                                aaGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCg                                                   >  6:333848/1‑133 (MQ=255)
                                                                                aaGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGa                                                  >  4:194498/1‑134 (MQ=255)
                                                                                 aGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAg                                               >  4:108805/1‑136 (MQ=255)
                                                                                   gctGTCTGGGCAGAGTAAGCCGCTCTCCTCGCCTGATTCCCCATGCCAGAAACGCCTGTGAACCCACAAAAATTTTTTTGGAGTTGATTTTCCATGGCCAAAATCGCCCGGCAATTGCCAATCGAAGTCGACAg                                               <  4:210825/132‑1 (MQ=255)
                                                                                   gATGGCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGAAAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCg                                             <  3:290407/136‑1 (MQ=255)
                                                                                     tGTCTGGGGCGAGTACGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCgag                                           <  3:159440/136‑1 (MQ=255)
                                                                                     tGTCTGGGGCGAGTAAGCCTCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCga                                            <  7:253071/135‑1 (MQ=255)
                                                                                     tGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCga                                            <  6:364920/135‑1 (MQ=255)
                                                                                       tCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCgag                                           <  5:310857/134‑1 (MQ=255)
                                                                                        cTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCgaga                                          <  8:282282/134‑1 (MQ=255)
                                                                                          ggggCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAAtttt                                                                                                        <  6:358040/70‑1 (MQ=255)
                                                                                          ggggCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAAtttt                                                                                                        >  5:358040/1‑70 (MQ=255)
                                                                                          ggggCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCgaga                                          <  4:241405/132‑1 (MQ=255)
                                                                                          ggggCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCgaga                                          >  3:241405/1‑132 (MQ=255)
                                                                                          ggggCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCg                                       >  2:290370/1‑135 (MQ=255)
                                                                                          ggggCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCg                                       >  6:119139/1‑135 (MQ=255)
                                                                                          ggggCGAGTAAGCATCTCTCCAAGCCTGATAGCCCCTGACAGCAACGCCGGGGCACCCACAAAAAATTTTATTGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCTACAGCGAGAcct                                       >  6:255363/1‑134 (MQ=255)
                                                                                           gggCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCgg                                      >  1:128607/1‑135 (MQ=255)
                                                                                            ggCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGgt                                     <  7:187639/135‑1 (MQ=255)
                                                                                               gAGTATGCCGCTATCAAAGCGTGAAAGCCCCTGCCAGAACCGCCGGGGCACCCACAAAAAATTTTATGGAGTTGATTTTCCATGGCCAAAATCGCCCCGCAATAGCCAATCGAAGTCGACAGCTAGACCGGTGTc                                  <  2:291288/135‑1 (MQ=255)
                                                                                               gAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTc                                  >  8:133234/1‑135 (MQ=255)
                                                                                                   aaGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAATAATTTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGAc                             >  3:157197/1‑136 (MQ=255)
                                                                                                   aaGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCga                                            <  5:159378/121‑1 (MQ=255)
                                                                                                   aaGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCga                                            >  6:159378/1‑121 (MQ=255)
                                                                                                   aaGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGa                              >  5:25294/1‑135 (MQ=255)
                                                                                                   aaGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGa                              >  7:292830/1‑135 (MQ=255)
                                                                                                    aGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGa                              >  6:127157/1‑134 (MQ=255)
                                                                                                            tcCAAGCCTGATAGCCCCTCCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGa                       <  1:130001/133‑1 (MQ=255)
                                                                                                            tcCAAGCCTGATAGCCCCTCCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGa                       >  2:130001/1‑133 (MQ=255)
                                                                                                             ccAAGCCTGATCGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAACTTTTATTGAGTTGAGTTTCCATTGCCAAAATCGCCCCGCAATTGCCAATCGCAGTCGACAGCGCGCCCGGTGTCTGGACCAGcgccgc                    >  8:174354/1‑135 (MQ=255)
                                                                                                             ccAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGcc                   >  4:112217/1‑136 (MQ=255)
                                                                                                                 gCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCANTCNAAGTCGa                                                  >  1:244635/1‑101 (MQ=255)
                                                                                                                 gCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGa                                                  <  2:244635/101‑1 (MQ=255)
                                                                                                                  ccTGATAGCCCCGGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGc               <  1:105090/135‑1 (MQ=255)
                                                                                                                   cTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCg             <  3:28931/136‑1 (MQ=255)
                                                                                                                    tgcaaGCCCCATCCAGCAACTCCGGGGCACCCACAAAAAATGTTATGCAGTTGAGTTTCCATGGCCAAAATCGCCACGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCg             <  6:48343/131‑1 (MQ=255)
                                                                                                                    tGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCg             <  8:96500/135‑1 (MQ=255)
                                                                                                                    tGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCg             <  8:104426/135‑1 (MQ=255)
                                                                                                                         gCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCt        >  2:16002/1‑135 (MQ=255)
                                                                                                                         gCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCt        >  5:12730/1‑135 (MQ=255)
                                                                                                                         gCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCTg       >  3:95984/1‑136 (MQ=255)
                                                                                                                              tGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCTGTAtg   <  1:16712/135‑1 (MQ=255)
                                                                                                                               gCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCTGTAtgt  >  2:353/1‑135 (MQ=255)
                                                                                                                               gCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCTGTAtgt  >  5:100683/1‑135 (MQ=255)
                                                                                                                               gCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCTGTAtgt  >  6:201681/1‑135 (MQ=255)
                                                                                                                               |                                                                                                                                      
TCACCGAGACCTTGCTCAAACGCGGCCATTCCGAGCGCGTGGTGCGCAAGATCATGGGCGAGAACTGGGTAAACGTCCTCAAGGATGTCTGGGGCGAGTAAGCCGCTCTCCAAGCCTGATAGCCCCTGCCAGCAACGCCGGGGCACCCACAAAAAATTTTATGGAGTTGAGTTTCCATGGCCAAAATCGCCCCGCAATTGCCAATCGAAGTCGACAGCGAGACCGGTGTCTGGACCAGCGACGCCTTGCCGATGCTGTATGT  >  KT2440/362848‑363109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑